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http://dx.doi.org/10.5395/JKACD.2009.34.6.491

COMPARISON OF VIABILITY OF ORAL EPITHELIAL CELLS STORED BY DIFFERENT FREEZING METHODS  

Baek, Do-Young (Department of Conservative Dentistry, College of Dentistry, Yonsei, University)
Lee, Seung-Jong (Department of Conservative Dentistry, College of Dentistry, Yonsei, University)
Jung, Han-Sung (Department of Conservative Dentistry, College of Dentistry, Yonsei, University)
Kim, Eui-Seong (Department of Conservative Dentistry, College of Dentistry, Yonsei, University)
Publication Information
Restorative Dentistry and Endodontics / v.34, no.6, 2009 , pp. 491-499 More about this Journal
Abstract
This study examined the influence of the storage methods on the viability of oral epithelial cells using conventional cell freezing storage, slow freezing preservation, rapid freezing preservation, and slow freezing preservation with a pressure of 2 Mpa or 3 Mpa. The cell viability was evaluated by cell counting, WST-1 and the clonogenic capacity after 6 days of freezing storage. After 6 days, the frozen cells were thawed rapidly, and the cell counting. WST-1, and clonogenic capacity values were measured and compared. 1. The results from cell counting demonstrated that conventional cryopreservation, slow freezing under a 2 Mpa pressure and slow freezing under a 3 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p < 0.05). 2. The results from the optical density by WST-1 demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05). 3. The clonogenic capacity demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p < 0.05).
Keywords
WST-1; Cell counting; Clonogenic capacity; Slow freezing under pressure; Slow freezing; Rapid freezing;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Farrant J. Water transport and cell survival in cryobiological procedures. Philos Trans R Soc Lond B Biol Sci 278 (959):191-205, 1977   DOI
2 Alotto D, Ariotti S, Graziano S, Verrua R, Stella M, Magliacani G, Castagnoli C. The role of quality control in a skin bank: tissue viability determination. Cell and Tissue banking 3(1):3-10, 2002   DOI
3 Czochrowska EM, Stenvik A, et al.'Outcome of tooth transplantation: survival and success rates 17-41 years posttreatment.' Am J Orthod Dentofacial Orthop 121 (2):110-9;quiz 193, 2002   DOI   ScienceOn
4 Abe F, Kato C, Horikoshi K. Pressure regulated metabolism in microorganisms. Trends Microbiol 7 (11):447-453, 1999   DOI   ScienceOn
5 Pelham HR. Heat shock proteins: coming in from cold. Nature 332:776-7, 1988   DOI   PUBMED   ScienceOn
6 Du Y, Lin L, Schmidt M, Bogh IB, Kragh PM, Sorensen CB, Li J, Purup S, Pribenszky C, Molnar M, Kuwayama M, Zhang X, Yang H, Bolund L, Vajta G. High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival. Cloning and stem cells 10(3):325-30, 2008   DOI   PUBMED   ScienceOn
7 Diller KR, Cravalho EG. A cryomicroscope for the study of freezing and thawing processes in biological cells. Cryobiology 7 (4):191-9, 1971   DOI
8 Berridge MV, Tan AS, McCoy KD, Wang R. The biochemical and cellular basis of cell proliferation assays that use tetrazolium salts. Biochemica 4:15-20, 1996
9 Rapatz G, Sullivan JJ, et al. 'Preservation of erythrocytes in blood containing various cryoprotective agents, frozen at various rates and brought to a given final temperature.' Cryobiology 5(1):18-25, 1968   DOI   ScienceOn
10 Mazur P. 'Equilibrium, quasi-equilibrium, and nonequilibrium freezing of mammalian embryos.' Cell Biophys 17(1):53-92, 1990   DOI   PUBMED   ScienceOn
11 Jonsson T, Sigurdsson TJ. 'Autotransplantation of premolars to premolar sites. A long-term follow-up study of 40 consecutive patients.' Am J Orthod Dentofacial Orthop 125(6):668-75, 2004   DOI   ScienceOn
12 Pribenszky C, Molnar M, Cseh S, Solti L. Improving post-thaw survival of cryopreserved mouse blastocysts by hydrostatic pressure challenge. Anim Reprod Sci 87(2):143-50, 2005   DOI   ScienceOn
13 Mazur P, Leibo SP, Chu EH. A two-factor hypothesis of freezing injury. Evidence from Chinese hamster tissue-culture cells. Exp Cell Res 71(2):345-55, 1972   DOI   ScienceOn
14 Thorpe PE, Knight SC, et al. 'Optimal conditions for the preservation of mouse lymph node cells in liquid nitrogen using cooling rate techniques.' Cryobiology 13(2):126-33, 1976   DOI   ScienceOn
15 Pribenszky C, Molnar M, Ulrich P, et al. Pressure assisted cryopreser vation. Reprod Dom Anim 40:228-344, 2005   DOI   ScienceOn
16 Leibo SP, McGrath JJ, et al. 'Microscopic observation of intracellular ice formation in unfertilized mouse ova as a function of cooling rate.' Cryobiology 15(3):257-71, 1978   DOI   ScienceOn
17 이영은, 김의성, 김진, 한승훈, 이승종. 압력 저속 냉동 방법의 쥐 치아 치주인대세포 보존 효율 평가. 대한치과보존학회지 34:356-363, 2009   DOI
18 Mazur P. Freezing of living cells: mechanisms and implications. Am J Physiol 247:125-42, 1984.
19 Fujikawa S. 'Freeze-fracture and etching studies on membrane damage on human erythrocytes caused by formation of intracellular ice.' Cryobiology 17 (4):351-62, 1980   DOI   ScienceOn
20 Kawata T. Tooth transplantation by teeth bankapproach to human-Hiroshima. Department of Orthodontics, Hiroshima University School of Dentistry, 2005
21 김재욱, 김의성, 김진, 이승종. 급속냉동된 쥐 치아의 in vivo MTT 검색법을 이용한 치주인대세포 활성도 평가. 대한치과보존학회지 31: 192-202, 2006   과학기술학회마을   DOI
22 안현정, 김의성, 김진, 김덕원, 김기열, 이찬영, 이승종. 자기장 저속 냉동보관법을 이용한 쥐 치아 치주인대세포의 활성도 검사. 대한치과보존학회지 33:332-240, 2008   DOI
23 Kuo YH, Pribenszky Cs, Huang SY. Higher litter size is achieved by the insemination of high hydrostatic pressure-treated frozen-thawed boar semen. Theriogenology 70:1395-96, 2008   DOI   ScienceOn
24 Aldridge BE, Bruner LJ. Pressure effects on mechanisms of charge transport across bilayer membranes. Biochem Biophys Acta 817 (2):343-354, 1985   DOI   ScienceOn
25 Huang SY, Pribenszky C, Kuo YH, et al. The effect of hydrostatic pressure treatment on the protein profile of boar spermatozoa before and after freezing. Theriogenology 70:1391, 2008   DOI   ScienceOn
26 Parsell DA, Lindquist S. The function of heat shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Ann Rev Genet 27:437-96, 1993   DOI   ScienceOn