Browse > Article
http://dx.doi.org/10.5395/rde.2014.39.1.39

Cytotoxicity of newly developed pozzolan cement and other root-end filling materials on human periodontal ligament cell  

Song, Minju (Department of Conservative Dentistry Gangnam Severance Dental Hospital)
Yoon, Tae-Sun (Department of Conservative Dentistry, Yonsei University College of Dentistry)
Kim, Sue-Youn (Department of Conservative Dentistry, Yonsei University College of Dentistry)
Kim, Euiseong (Microscope Center, Department of Conservative Dentistry and Oral Science Research Center, Yonsei University College of Dentistry)
Publication Information
Restorative Dentistry and Endodontics / v.39, no.1, 2014 , pp. 39-44 More about this Journal
Abstract
Objectives: The purpose of this study was to evaluate in vitro cytotoxicity of the pozzolan cement and other root-end filling materials using human periodontal ligament cell. Materials and Methods: Endocem (Maruchi), white ProRoot MTA (Dentsply), white Angelus MTA (Angelus), and Super EBA (Bosworth Co.) were tested after set completely in an incubator at $37^{\circ}C$ for 7 days, Endocem was tested in two ways: 1) immediately after mixing (fresh specimens) and 2) after setting completely like other experimental materials. The methods for assessment included light microscopic examination, cell counting and WST-1 assay on human periodontal ligament cell. Results: In the results of microscopic examination and cell counting, Super EBA showed significantly lower viable cell than any other groups (p < 0.05). As the results of WST-1 assay, compared with untreated control group, there was no significant cell viability of the Endocem group. However, the fresh mixed Endocem group had significantly less cell viability. The cells exposed to ProRoot MTA and Angelus MTA showed the highest viability, whereas the cells exposed to Super EBA displayed the lowest viability (p < 0.05). Conclusions: The cytotoxicity of the pozzolan cement (Endocem) was comparable with ProRoot MTA and Angelus MTA. Considering the difficult manipulation and long setting time of ProRoot MTA and Angelus MTA, Endocem can be used as the alternative of retrofilling material.
Keywords
Cytotoxicity; Endocem; Pozzolan cement; Root-end filling material; WST-1 assay;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ishiyama M, Shiga M, Sasamoto K, Mizoguchi M, He PG. A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem Pharm Bull 1993;41:1118-1122.   DOI   ScienceOn
2 De Deus G, Ximenes R, Gurgel-Filho ED, Plotkowski MC, Coutinho-Filho T. Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells. Int Endod J 2005;38:604-609.   DOI   ScienceOn
3 Saidon J, He J, Zhu Q, Safavi K, Spangberg LS. Cell and tissue reactions to mineral trioxide aggregate and Portland cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;95:483-489.   DOI   ScienceOn
4 Shin SJ. In vitro studies addressing celluar mechanisms underlying the bone and dentin inductive property of mineral trioxide aggregate (MTA). Master thesis in oral Biology. University of Pennsylvania; 2004. p75.
5 Lin CP, Chen YJ, Lee YL, Wang JS, Chang MC, Lan WH, Chang HH, Chao WM, Tai TF, Lee MY, Lin BR, Jeng JH. Effects of root-end filling materials and eugenol on mitochondrial dehydrogenase activity and cytotoxicity to human periodontal ligament fibroblasts. J Biomed Mater Res B Appl Biomater 2004;71:429-440.
6 Bonson S, Jeansonne BG, Lallier TE. Root-end filling materials alter fibroblast differentiation. J Dent Res 2004;83:408-413.   DOI
7 Moghaddame-Jafari S, Mantellini MG, Botero TM, McDonald NJ, Nor JE. Effect of ProRoot MTA on pulp cell apoptosis and proliferation in vitro. J Endod 2005;31: 387-391.   DOI   ScienceOn
8 Song M, Kim SG, Shin SJ, Kim HC, Kim E. The influence of bone tissue deficiency on the outcome of endodontic microsurgery: a prospective study. J Endod 2013;39:1341-1345.   DOI
9 Karimjee CK, Koka S, Rallis DM, Gound TG. Cellular toxicity of mineral trioxide aggregate mixed with an alternative delivery vehicle. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;102:e115-120.   DOI
10 Huang FM, Chang YC. Cytotoxicity of resin-based restorative materials on human pulp cell cultures. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002; 94:361-365.   DOI
11 Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/ additives in permanent 3T3 and three human primary fibroblast cultures. J Biomed Mater Res 1998;41:474-480.   DOI
12 Perez AL, Spears R, Gutmann JL, Opperman LA. Osteoblasts and MG-63 osteosarcoma cells behave differently when in contact with ProRoot MTA and White MTA. Int Endod J 2003;36:564-570.   DOI
13 Abdullah D, Ford TR, Papaioannou S, Nicholson J, McDonald F. An evaluation of accelerated Portland cement as a restorative material. Biomaterials 2002;23: 4001-4010.   DOI   ScienceOn
14 Balto HA. Attachment and morphological behavior of human periodontal ligament fibroblasts to mineral trioxide aggregate: a scanning electron microscope study. J Endod 2004;30:25-29.   DOI   ScienceOn
15 Torabinejad M, Hong CU, McDonald F, Pitt Ford TR. Physical and chemical properties of a new root-end filling material. J Endod 1995;21:349-353.   DOI   ScienceOn
16 Zhu Q, Haglund R, Safavi KE, Spangberg LS. Adhesion of human osteoblasts on root-end filling materials. J Endod 2000;26:404-406.   DOI   ScienceOn
17 Asgary S, Eghbal MJ, Parirokh M, Ghoddusi J, Kheirieh S, Brink F. Comparison of mineral trioxide aggregate's composition with Portland cements and a new endodontic cement. J Endod 2009;35:243-250.   DOI   ScienceOn
18 Dorn SO, Gartner AH. Retrograde filling materials: A retrospective success-failure study of amalgam, EBA, and IRM. J Endod 1990;16:391-393.   DOI   ScienceOn
19 Torabinejad M, Hong CU, Lee SJ, Monsef M, Pitt Ford TR. Investigation of mineral trioxide aggregate for rootend filling in dogs. J Endod 1995;21:603-608.   DOI   ScienceOn
20 Rubinstein RA, Kim S. Long-term follow-up of cases considered healed one year after apical microsurgery. J Endod 2002;28:378-383.   DOI   ScienceOn
21 Taschieri S, Del Fabbro M, Testori T, Weinstein R. Endoscopic periradicular surgery: a prospective clinical study. Br J Oral Maxillofac Surg 2007;45:242-244.   DOI
22 Enkel B, Dupas C, Armengol V, Akpe Adou J, Bosco J, Daculsi G, Jean A, Laboux O, LeGeros RZ, Weiss P. Bioactive materials in endodontics. Expert Rev Med Devices 2008;5:475-494.   DOI
23 Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review-Part I: chemical, physical, and antibacterial properties. J Endod 2010;36: 16-27.   DOI   ScienceOn
24 Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review-Part III: Clinical applications, drawbacks, and mechanism of action. J Endod 2010;36:400-413.   DOI   ScienceOn
25 Torabinejad M, Parirokh M. Mineral trioxide aggregate: a comprehensive literature review-part II: leakage and biocompatibility investigations. J Endod 2010;36:190-202.   DOI   ScienceOn
26 Samara A, Sarri Y, Stravopodis D, Tzanetakis GN, Kontakiotis EG, Anastasiadou E. A comparative study of the effects of three root-end filling materials on proliferation and adherence of human periodontal ligament fibroblasts. J Endod 2011;37:865-870.   DOI
27 Torabinejad M, Hong CU, Pitt Ford TR, Kaiyawasam SP. Tissue reaction to implanted super-EBA and mineral trioxide aggregate in the mandible of guinea pigs: a preliminary report. J Endod 1995;21:569-571.   DOI   ScienceOn
28 Al-Saeed OR, Al-Hiyasat AS, Darmani H. The effects of six root-end filling materials and their leachable components on cell viability. J Endod 2008;34:1410-1414.   DOI
29 Bondra DL, Hartwell GR, MacPherson MG, Portell FR. Leakage in vitro with IRM, high copper amalgam, and EBA cement as retrofilling materials. J Endod 1989; 15:157-160.   DOI
30 Bodrumlu E. Biocompatibility of retrograde root filling materials: a review. Aust Endod J 2008;34:30-35.   DOI   ScienceOn
31 Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y, Ueno K. A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. Biol Pharm Bull 1996;19:1518-1520.   DOI   ScienceOn