• 미생물학회지
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• 제55권3호
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• pp.313-315
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• 2019

사일리지 제조에 사용하기 위한 섬유소 분해균을 탐색하는 중 절대혐기성 세균인 WCF-2 균주를 선발하였다. WCF-2 균주는 16S rRNA 유전자 염기서열 유사도가 가장 높은(98.2%) 표준균주인 Cellulosilyticum lentocellum DSM $5427^T$ 보다 높은 섬유소 분해 활성을 나타내었다. WCF-2 균주의 전체 유전체 염기서열을 분석하고 이를 C. lentocellum DSM $5427^T$와 비교하였을 때 두 균주의 OrthoANI 값은 97.9%로 나타나 WCF-2를 C. lentocellum으로 동정하였다. WCF-2 균주의 유전체 크기는 4,779,774 bp이고 G + C 함량은 34.4%였으며 4,154개의 단백질 암호화 유전자 및 142개의 RNA 암호화 유전자를 보유하고 있었다. 또한 WCF-2 균주는 7개의 cellulase를 보유하고 있었으며 이 중 5개는 C. lentocellum DSM $5427^T$의 cellulase와 낮은 유사도를 나타내었다.

• 터널과지하공간
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• 제17권3호
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• pp.234-243
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• 2007

본 연구에서는 시추공 조사 결과를 바탕으로 수리지질학적 모델 구축과정에 요구되는 해석과정의 체계화 및 고도화의 일환으로 균열 암반에서의 조사 시추공을 통해 얻어진 균열 정보로부터 지하수 유동경로를 제공하는 구조(Water Conducting Feature, WCF)를 선별하고, 선별된 WCF 정보를 이용하여 HydroDFN 모델을 구축하였다. HydroDFN 모델 내부를 세분한 정육면체 블록에서의 등가투수계수를 계산하고, 계산된 등가블록투수계수는 시추공 투수시험 결과와 비교함으로써 구축된 HydroDFN 모델의 검증에 이용하였다. 이러한 해석과정을 통해 투수량 계수 등으로 대표되는 WCF 파라미터의 설정 및 수리지질학적 모델 구축시의 효과적인 방안을 제안하였다.

• Advances in nano research
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• 제14권6호
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• pp.557-573
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• 2023

The rapid growth of zinc-oxide (ZnO) nanostructures (NSs) on woven carbon fiber (WCF) is reported in this study employing a microwave-aided chemical bath deposition process. The effects of different process parameters such as molar concentration, microwave duration and microwave power on morphologies and growth rate of the ZnO on WCF were studied. Furthermore, an attempt has been taken to study influence of different type of growth solutions on ZnO morphologies and growth rates. The surface functionalization of WCF fabrics is achieved by successful growth of crystalline ZnO on fiber surface in a very short duration through one-step microwave synthesis. The morphological, structural and compositional studies of ZnO-modified WCF are evaluated using field-emission scanning electron microscopy, X-ray diffraction and energy dispersive X-ray spectroscopy respectively. Good amount of zinc and oxygen has been seen in the surface of WCF. The presence of the wurtzite phase of ZnO having crystallite size 30-40 nm calculated using the Debye Scherrer method enhances the surface characteristics of WCF fabrics. The UV-VIS spectroscopy is used to investigate optical properties of ZnO-modified WCF samples by absorbance, transmittance and reflectance spectra. The variation of different parameters such as dielectric constants, optical conductivity, refractive index and extinction coefficient are examined that revealed the enhancement of optical characteristics of carbon fiber for wide applications in optoelectronic devices, carbon fiber composites and photonics.

• 한국식품저장유통학회지
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• 제11권1호
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• pp.34-37
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• 2004

유청칼슘을 0.5%, 1.0% 및 1.5%씩 첨가하여 만든 김치를 1$0^{\circ}C$에서 숙성시키면서 pH, 적정산도, 환원당 함량, 총균수 및 유산균수를 측정한 결과는 다음과 같았다. 김치의 담금 직후부터 유청칼슘을 첨가한 시험구가 대조구보다 높은 pH를 유지하였다. 숙성 5일까지는 시험구간에 적정산도의 차이가 거의 나타나지 않았으나 그 이후에는 유청칼슘을 첨가한 시험구의 적정산도가 대조구보다 모두 높았다. 특히 유청칼슘의 첨가량이 많을수록 적정산도가 높았다. 유청칼슘을 첨가한 시험구는 김치 담금시부터 총균수 및 유산균가 많았으며, 유청칼슘의 첨가량이 많을수록 총균수 및 유산균수가 더 많았다.

• 한국시뮬레이션학회논문지
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• 제16권4호
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• pp.33-41
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• 2007

서비스 지향 아키텍처는 하나의 실체가 다른 하나의 실체를 대신해 단위 작업을 수행하도록 하는 방식으로 두 개의 컴퓨팅 실체가 상호작용 하는 방법을 정의한다. 이 단위 작업은 "서비스"라고 지칭되며, 이 서비스 상호작용들은 독립적이고 느슨한 결합을 가져야 한다. SOA의 이러한 특징인 느슨한 결합과, 높은 상호운영성의 효과는 서로 다른 이용자들이 서로 다른 방식으로 서비스와 의사소통을 하기 위해 얼마나 유연한 메시지 통신이 가능한가에 따라 결정된다. 본 논문에서는 .NET Framework를 이용한 WCF 기반의 메시징 프레임워크와 통합된 데이터 표현 방식을 이용하여 서비스 지향 아키텍처를 기반으로 한 웹 서비스 애플리케이션과 일반적인 웹 서비스에 대해 애플리케이션의 응답 성능 및 시스템의 안정성과 성능 지수를 비교 분석하였으며 그 결과를 통하여 WCF를 이용한 SOA환경의 웹 서비스가 가지는 장점을 확인하였다.

• 한국축산식품학회지
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• 제39권6호
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• pp.953-965
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• 2019

In this study, the Opuntia humifusa f. jeollaensis (Wanggasi-Chunnyuncho) fruit (WCF) was used as a source of viscous dietary fiber and color pigmentation in sausage production to improve quality characteristics, including cooking loss and emulsion stability. Control and treatment sausages were formulated with 0%, 1%, 5%, and 10% WCF powder, respectively, and the following quality measures were investigated: general composition, fiber content, cooking loss, emulsion stability, chromaticity, pH, texture, and sensory properties. The moisture, dietary fiber, and ash contents showed increasing trends (all p<0.05) with increasing concentrations of fruit powder. Conversely, crude protein and crude fat contents exhibited decreasing trends with increasing fruit powder concentrations (p<0.05). Moreover, both the moisture and dietary fiber contents affected cooking loss, water retention capacity, and the sausage texture, causing the cooking loss to decrease, while the water and oil retention capacity increased (p<0.05) with increasing concentrations of WCF powder. Furthermore, hardness exhibited a significant decreasing trend as the concentration of WCF powder increased (p<0.05). This finding suggested that both dietary fiber and viscous materials, along with the protein and moisture content, affected the product hardness. In addition, sensory evaluation of the WCF powder groups showed better results than did the control group, and the strongest results obtained for the group containing 5% fruit powder (p<0.05). Therefore, this study suggests that WCF powder improves the quality of emulsified sausages and can potentially be applied as a naturally-derived additive.

• 대한약침학회지
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• 제17권1호
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• pp.27-34
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• 2014

Objectives: This experiment was conducted to examine the toxicity of Water soluble Carthmi-Flos herbal acupuncture (WCF) by administering a single intramuscular dose of WCF in 6-week-old, male and female Sprague-Dawley rats and to find the lethality dose for WCF. Methods: The experiment was conducted at Biotoxtech according to Good Laboratory Practices under a request by the Korean Pharmacopuncture Institute. This experiment was performed based on the testing standards of "Toxicity Test Standards for Drugs" by the Ministry of Food and Drug Safety. Subjects were divided into 4 groups: 1 control group in which normal saline was administered and 3 test groups in which 0.1, 0.5 or 1.0 mL of WCF was administered; a single intramuscular dose was injected into 5 males and 5 females in each group. General symptoms and body weights were observed/measured for 14 days after injection. At the end of the observation period, hematological and clinical chemistry tests were performed, followed by necropsy and histopathological examinations of the injected sections. Results: No mortalities were observed in any group. Also, symptoms, body weight, hematology, clinical chemistry and necropsy were not affected. However, histopathological examination of the injected part in one female in the 1.0-mL group showed infiltration of mononuclear cells and a multi-nucleated giant cell around eosinophilic material. Conclusion: Administration of single intramuscular doses of WCF in 3 groups of rats showed that the approximate lethal dose of WCF for all rats was in excess of 1.0 mL, as no mortalities were observed for injections up to and including 1.0 mL.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2019년도 춘계학술대회
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• pp.147-148
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• 2019

본 논문에서는 건설을 업무로 하는 회사의 클라우드 기반 현장 중심의 ERP 개발에 대하여 제시한다. ERP 개발은 웹기반의 사용자 서비스 포탈 및 관리 포탈 개발, WCF(윈도 커뮤티케이션 파운데이션)기반의 미들웨어 개발, 그리고 WPF(윈도 프리젠테이션 파운데이션) 기반의 클라이언트 소프트웨어 개발을 목표로 수행하였다. ERP의 주요 개발 내용은 소규모 기업의 건설 회사용 프로세스 제정, 사용자 중심의 인터페이스 설계, 프로세스 집약화와 오토메이션이다.

• 대한약침학회지
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• 제17권3호
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• pp.31-39
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• 2014

Objectives: This study was performed to analyze the toxicity and to find the lethal dose of the test substance Water-soluble Carthami-flos pharmacopuncture (WCF) when used as a single intravenous-dose in 6-week-old, male and female Sprague-Dawley rats. Methods: The experiment was conducted at Biotoxtech according to Good Laboratory Practices. 20 female and 20 male Spague-Dawley rats were divided into 4 groups of 5 female and 5 male animals per group. The rats in the three experimental groups received single intravenous injections with 0.125-mL, 0.25-mL and 0.5-mL/animal doses of WCF, Groups 2, 3, and 4, respectively, and the control group, Group 1, received a single intravenous injection with a 0.5-mL dose of normal saline. Clinical signs were observed and body weight measurements were carried out for 14 days following the injections. At the end of the observation period, hematology, clinical chemistry, histopathological tests and necropsy were performed on the injected parts. Results: No deaths occurred in any of the groups. Also, no significant changes in body weight, hematological parameters or clinical chemistry test results between the control group and the experimental groups were observed. Visual inspection after necropsy showed no abnormalities. Histopathological tests on the injected parts showed no significant differences, except for Group 1 females; however, the result was spontaneous generation and had no toxicological meaning because it was not dose-dependent. Therefore, this study showed that WCF had no effect on the injected parts in terms of clinical signs, body weight, hematology, clinical chemistry, and necropsy. Conclusion: As a result of single intravenous-dose tests of the test substance WCF in 4 groups of rats, the lethal dose for both males and females exceeded 0.5 mL/animal. Therefore, WCF is a relatively safe pharmacopuncture that can be used for treatment, but further studies should be performed.

• 대한약침학회지
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• 제18권1호
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• pp.36-43
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• 2015

Objectives: Water-soluble carthami flos (WCF) is a new mixture of Carthami flos (CF) pharmacopuncture. We conducted a 4-week toxicity test of repeated intramuscular injections of WCF in Sprague-Dawley rats. Methods: Forty male and 40 female rats were divided into 4 groups of 10 male and 10 female SD rats: The control group received 0.5 mL/animal/day of normal saline whereas the three experimental groups received WCF at doses of 0.125, 0.25, and 0.5 mL/animal/day, respectively. For 4 weeks, the solutions were injected into the femoral muscle of the rats alternating from side to side. Clinical signs, body weights, and food consumption were observed; opthalmological examinations and urinalyses were performed. On day 29, blood samples were taken for hematological and clinical chemistry analyses. Then, necropsy was conducted in all animals to observe weights and external and histopathological changes in the bodily organs. All data were tested using a statistical analysis system (SAS). Results: No deaths were observed. Temporary irregular respiration was observed in male rats of the experimental group for the first 10 days. Body weights, food consumptions, opthalmological examinations, urinalyses, clinical chemistry analyses, organ weights and necropsy produced no findings with toxicological meaning. In the hematological analysis, delay of prothrombin time (PT) was observed in male rats of the 0.25- and the 0.5-mL/animal/day groups. In the histopathological test, a dose-dependent inflammatory cell infiltration into the fascia and panniculitis in perimuscular tissues was observed in all animals of the experimental groups. However, those symptoms were limited to local injection points. No toxicological meanings, except localized changes, were noted. Conclusion: WCF solution has no significant toxicological meaning, but does produce localized symptoms. No observed adverse effect level (NOAEL) of WCF in male and female rats is expected for doses over 0.5 mL/animal/day.