• Journal of Life Science
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• v.25 no.10
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• pp.1148-1155
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• 2015

Commercial complete feeds contain enough nutrients to support animal growth and it is easy to be spoiled under proper temperature and humid conditions. The aim of this study was to investigate microbiological and chemical changes on complete feed for milking cow under open-air exposure with moisture 33% at 30℃ during 15 days. pH decreased 6.29 to 4.66 and water activity decreased gradually 0.99 to 0.95. Bacteria increased 6.2×10⁶~1.6×10⁷ to 2.1×10⁹ CFU/g at 5 days and showed 10⁸ CFU/g until 15 days. Fungi increased 10³ CFU/g to 8.0×10⁴ CFU/g. During the processing of spoilage, bacteria such as Acinetobacter oleivorans, Pediococcus acidilactici, Acinetobacter oleivorans, Weissella cibaria, and Methylobacterium komagatae were identified and fungi such as Fusarium sp. and Mucor sp. were also identified. Moisture content increased until 10 days (p<0.01). Crude protein was not changed so much whereas crude fat decreased 6.0% to 5.5% (p<0.01). Crude fiber and crude ash changed 2.0~ 3.0% and 4.5~ 4.8% levels with no significance, respectively. Gross energy was not almost changed at 4,400 kcal/g. During spoilage, lactate and propionate increased whereas acetate was not detected. Protease and lipase activities increased significantly during spoilage (p<0.01). Zearalenone content increased 59.2 μg/kg to 623.8 μg/kg, showing 10.5 times more production. During feed spoilage, pH decreased with microbial growth and various chemical changes were occurred.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.6
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• pp.429-433
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• 2003

trans-Resveratrol(3,5,4'-trihydroxystilbene) is phenolic compound present in grapes, wines, and peanuts, has been reported to have health benefits including anticarcinogenic effects, protection against cardiovascular diseases and reduced cancer risk. A simple method for the quantitative extraction of trans-resveratrol from peanut has been developed. Optimal conditions for extraction were investigated. Type of solvent, time, and temperature assayed influenced trans-resveratrol yield. Adequate extraction condition was decided to ethanol/water (80:20v/v) maintained at $25^{\circ}C$ for 45 min. After extraction, the protocol consists of sample preparation using a $\textrm{C}_{18}$ solid-phase extraction (SPE) cartridge after concentrate with rotary evaporator and quantified by reversed phase HPLC using a $\textrm{C}_{18}$ column at 308 nm. Analytical methods for measuring trans-resveratrol in peanut were adapted to isolate, identify, and quantify trans-resveratrol in 11 peanut varieties by HPLC (high-performance liquid chromatography) with UV detector, The 11 peanut varieties content ranged from 0.018 to 1.125 $\mu\textrm{g}/\textrm{g}$ with an average of 0.289 $\mu\textrm{g}/\textrm{g}$. The contents were higher in the seeds with than without testa, regardless of varieties. The trans-resveratrol content was Higher in 110, 130 days after sowing than that of other period.

• Korean Journal of Medicinal Crop Science
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• v.13 no.3
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• pp.81-86
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• 2005

The immune activities of the extracts from Ephedrae Herba and Rubi Frutus extraction with ultrasonification at $60^{\circ}C$ were compared with the extracts though water extraction at $100^{\circ}C$. The growth of human T cells was increased up to $13{\times}10^4\;viable\;cells/m{\ell}$ in adding $1.0\;g/{\ell}$ of the ultrasonification extracts of R. Fructus at $60^{\circ}C$, compared to adding the extracts at $100^{\circ}C$. The secretion of $TNF-{\alpha}$ for human T cells were also increased up to $13.9{\times}10^{-4}$ pg/cell by adding extracts at $60^{\circ}C$, compared to extracts at $100^{\circ}C$. The extracts of R. Fructus at $60^{\circ}C$ increased NK cell activities up to 50% and the secretion of $NO^{-1}$ from macrophage to $31\;{\mu}M$ for ultrasonification extracts at $60^{\circ}C$.

• Journal of Aquaculture
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• v.9 no.3
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• pp.223-232
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• 1996

Growth and survival rate in shortnecked clam, Ruditapes philippinarum were studied from different seedling production areas, Hadong, Ulsan and Kochang to Kwangyang Bay, Korea, and three stocking densities (1,000, 1,500 and 3,000 individuals/$m^2$) of clam from Ulsan seedling production area were examined. During the experimental period, water temperature and specific gravity were ranged from 4.5 to $26.0^{\circ}C$ and from 1.0216 to 1.0248, respectively. Shell length was grown from $2.30{\pm}0.05\;to\;3,29{\pm}0.02$ cm in Hadong, $1.32{\pm}0.02\;to\;3.15{\pm}2.02$ cm in Ulsan, $1.24{\pm}0.03\;to\;3.18{\pm}0.02$ cm in Kochang. Kochang's seed was displayed fast growth rate than others. In different rearing densities, the lower density group was showed a rapid growth rate. Shell length from the 1,000 individuals per $m^2$ group grew longer than that from the 2,000 individuals group. Total weight from the 1,000 individuals group was higher than that from the 1,5()0 individual and the 2,000 individual groups. Survival rate by seedling production areas, Hadong, Ulsan and Kochang showed $69.4\%,\;63.8\%\;and\;41.7\%$, respectively. Survival rate by rearing density, 1,000, 1,500 and 2,000 individual groups showed $70.8\%,\;67.8\%\;and\;59.0\%$, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.11
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• pp.1548-1555
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• 2011

Persimmons are shown to contain high levels of phenolics. The present study was designed to investigate if a sweet persimmon wine (SPW) would affect the development of alcoholic fatty liver in rats. Initially, male Sprague-Dawley rats were housed singly in stainless steel wire-bottomed cages in a room of controlled temperature and lighting. The rats had free access to a nutritionally adequate AIN-93G diet and deionized water. After the acclimatization period, rats were weight-matched and assigned to the following three groups: two groups were fed 6.7% ethanol or the caloric equivalent of maltose-dextrin in a Lieber-DeCarli diet and the other group was fed the isocaloric Lieber-DeCarli diet containing SPW at the same ethanol level. All three groups were fed their respective diets for 6 weeks. Serum transaminase, cholesterol, and triglyceride levels were measured. Liver lipids and histology were assessed at 6 weeks. The total phenolic content and the antioxidant and free radical scavenging activities of SPW were determined. SPW significantly increased antioxidant and free radical scavenging activities. As markers of liver injury, serum alanine and aspartate transminases were markedly lowered by SPW at 6 weeks. SPW significantly reduced the serum levels of serum cholesterol and triglyceride compared to ethanol treatment. SPW delayed the development of an alcoholic fatty liver by reversing fat accumulation in the liver, as evidenced in histological observations. Taken together, SPW seems to protect the liver from becoming fatty by alleviating fatty liver symptoms and lowering hepatic and serum lipid levels. Such a protective effect of SPW appears to be in part due to its phenolics.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.1
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• pp.68-75
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• 2013

The physiological properties of water extracts from Jerusalem artichoke (Helianthus tuberosus L.) leaves (JAL) with different extraction processes (stirrer extraction, SE; reflux extraction, RE; autoclave extraction, AE; low temperature high pressure extraction, LTPE) were investigated. The freeze-dried powder yields of SE, RE, AE, and LTPE were 22.33%, 29.88%, 31.65, and 15.74%, respectively. AE showed the highest value of extract yield. The $a^*$ and $b^*$ values were higher in AE compared to other extracts. Total polyphenolics and flavonoids contents in AE was significantly higher than in other extracts. The amount of proanthocyanidin related substances were highest in LTPE (29.36 mg/g), followed by RE (21.57 mg/g), SE (20.35 mg/g), and AE (13.02 mg/g). The electron donating abilities of SE, RE, AE, and LTPE at a concentration of $500{\mu}g/mL$ (w/v) were 76.16%, 39.55%, 25.50%, and 12.59%, respectively. Reducing power for the four different processes was 1.79, 1.60, 1.51, and 1.17, respectively. Additionally the same tendency was observed with electron donating ability and reducing power for ABTS radical and nitrite scavenging abilities. AE and LTPE showed relatively high antioxidant activities. Alpha-glucosidase, xanthine oxidase, and angiotensin I-converting enzyme inhibitory activities of LTPE at a concentration of $500{\mu}g/mL$ (w/v) were somewhat higher than other extracts. Additionally, there was significantly higher or little lower inhibitory activity compared to the control group. In conclusion, we provided experimental evidence that extracts of JAL have potential as functional materials, and component analysis of JAL could be used as new cosmeceuticals. Also, LTPE is the superior method for the enhancement of biological activity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.12
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• pp.2019-2027
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• 2013

In this study, we tried to develop a coating agent for the fresh-cut fruits used in cakes. First, the coating agent mixing ratios of sugar, pectin, sodium alginate, carrageenan, xanthan gum, vitamin C, and purified water were selected to be 55, 2, 2, 0.04, 0.1, 0.05, and 40.81% (w/w), respectively. In a freeze-thaw stability of the coating agent, the viscosity remained constant for 3 cycles of freezing and thawing repetition process, but showed a slightly decreasing trend in the 4th repetition process (P<0.05). On the other hand, the sugar content, pH, and chromaticity remained constant even in the 4th repetition process. Pineapple coated with the coating agent had smaller weight loss, hardness changes, and total bacteria distribution compared to the uncoated pineapple (P<0.05). In the chromaticity, both of the two pineapples experienced browning with increasing storage duration, as L value decreases and b value increases. However, when the color difference was compared, the progress of browning for the uncoated pineapple was faster than the coated pineapple. Also, the progress of browning at $4^{\circ}C$ was found to be slower than the progress of browning at $25^{\circ}C$. Therefore, the storage stability of the fresh-cut fruits could be improved by coating the fresh-cut fruits for cakes with the coating agent and storing at a low temperature, which would contribute to extending the shelf-life of cakes.

• Economic and Environmental Geology
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• v.19 no.2
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• pp.109-122
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• 1986

Mn-Pb-Zn-Ag deposits of the Janggun mine are hosted in the Cambro-Ordovician Janggun limestone mostly along the contacts of the Jurassic Chunyang granite. The deposits are represented by several ore pipes and steeply dipping lenticular bodies consisting of lower Pb-Zn-Ag sulfide ores and upper manganese carbonate and oxide ores. The former consists mainly of arsenic, antimony, silver, manganese, and tin-bearing sulfides, whereas the latter are characterized by hypogene rhodochrosite, and superficial manganese oxides including todorokite, nsutite, pyrolusite, cryptomelane, birnesite and janggunite. Origin of the upper manganese ore deposits has been a controversial subject among geologists for this mine: hydrothermal metasomatic vs. syngenetic sedimentary origin. Syngenetic advocators have proposed a new sedimentary rock, rhodochrostone, which is composed mainly of rhodochrosite in mineralogy. In the present study, carbon, oxygen and sulfur isotopic compositions were analayzed obtaining results as follows: Rhodochrosite minerals, (Mn, Ca, Mg, Fe) $CO_3$, from hydrothermal veins, massive sulfide ores and replacement ores in dolomitic limestone range in isotopic value from -4.2 to -6.3‰ in ${\delta}^{13}C$(PDB) and +7.6 to +12.9‰ in ${\delta}^{18}O$(SMOW) with a mean value of -5.3‰ in ${\delta}^{13}C$ and +10.7‰ in ${\delta}^{18}O$. The rhodochrosite bearing limestone and dolomitic limestone show average isotopic values of -1.5‰ in ${\delta}^{13}C$ and +17.5‰ in ${\delta}^{18}O$, which differ from those of the rhodochrosite mentioned above. This implies that the carbon and oxygen in ore fluids and host limestone were not derived from an identical source. ${\delta}^{34}S$ values of sulfide minerals exhibit a narrow range, +2.0 to +5.0‰ and isotopic temperature appeared to be about $288{\sim}343^{\circ}C$. Calculated initial isotopic values of rhodochrosite minerals, ${\delta}^{18}O_{H_2O}=+6.6$ to +10.6‰ and ${\delta}^{13}C_{CO_2}=-4.0$ to -5.1 ‰, strongly suggest that carbonate waters should be deep seated in origin. Isotopic data of manganese oxide ores derived from hypogene rhodochrosites suggest that the oxygen of the limestone host rock rather than those of meteoric waters contribute to form manganese oxide ores above the water table.

• Journal of Life Science
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• v.18 no.10
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• pp.1447-1454
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• 2008

Effect of heat processing on thermal stability of kudzu root isoflavone was investigated for future use such as various processed foods and functional foods. Kudzu root extracts were heated at 80, 100, 121, 140, 165, and $180^{\circ}C$ for up to 90 minutes before and after concentration, respectively. Changes in the amount of isoflavones were monitored using HPLC and thermal stability was investigated using Arrhenius equation. The amount of both daidzin and genistin decreased slightly during heating at 80, 100 and $121^{\circ}C$ but decreased significantly above $140^{\circ}C$. This indicated that daidzin and genistin are stable at temperatures near the boiling point of water. The degradation of both daidzin and genistin occurred in two steps and each step showed typical first order kinetic. The degradation rates were faster in the first step than the second step in both daidzin and genistin. Additionally, the degradation was accelerated when they heated after concentration compared to the sample heated before concentration. These results suggested that degradation of kudzu root isoflavone was highly dependent on both their concentration and heating temperature. This study provides the basic information on thermal stability of kudzu root isoflavones, which can be used for future processing of functional foods.

• Journal of the Korean Society for Marine Environment & Energy
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• v.11 no.2
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• pp.78-85
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• 2008

We estimated the pollutant loads for the last 3 years based on the daily discharge at the Nakdong River dam(barrage) and spatiotemporal characteristics of seawater quality in the Nakdong river estuary to investigate the correlation between the pollutant load inflow rate and seawater quality. The main results from this research are summarized as follows. (1) The total discharge at the Nakdong River dam dam the last 11 years has been $224,576.8{\times}10^6m^3/day$. The discharge figures show that the maximum discharge occurs in August with $52,634.2{\times}10^6 m^3/day$ (23.4% of the year's volume), followed by July and Sep. in that order with 23.1 and 17%, respectively. (2) The pollutant load influx from the Nakdong River dam was composed of 307,591.3COD-kg/day, 128.746.1 TN-kg/day, and 107,625.8 TP-kg/day. (3) The surface temperature in the Nakdong River estuary was about $2.137^{\circ}C$ higher than that of the lower layer. The salinity of the lower layer was 2.209%o higher than that of the ocean surface. The salinity of the ocean surface decreased by up to 19.593%o due to the inflow of the discharge at the Nakdong River dam. (4) DO, COD, TN, and SS concentration levels tended to be higher at the ocean surface than in lower layers, whereas the reverse was true for TP. (5) The water mass at the ocean's surface and in the lower layers during the drought and flood seasons tended to be separated by the difference in densities due to the freshwater inflow.