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Purification and Pysiological Characterization of Isoperoxidase from Oat Root Treated with Alachlor (Alachlor 처리후(處理後) 귀리 근단(根端)에 존재(存在)하는 동위과산화효소(同位過酸化酵素) 정제(精製) 및 효소(酵素)의 생리적(生理的) 특성(特性))

  • Kwon, S.W.;Han, K.S.;Kim, J.C.
    • Korean Journal of Weed Science
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    • v.14 no.1
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    • pp.56-61
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    • 1994
  • The cationic isoperoxidases were isolated from oat root tips which had been grown in treatment with $1{\times}10^{-4}$ M alachlor and purified about 30-fold by treatment with ethylalchol and ion exchange chromatography on DEAF-celluose and CM-sephadex medium. The oat root was found to contain three isoperoxidase. The major activity peak (B) represented 65% of the total isoperoxidase activity. After purification, the major peak of isoperoxidase was purified about 37-fold from the oat root. Analysis of the major peroxidase peak(column fraction 58-78) by SDS revealed a single band which corresponed to a molecular mass of 42.5 kD. In vitro, isoperoxidase activies were inhibited by IAA. Isoperoxidase(50 unit) significantly inhibited 70.2% of cell division in oat root and 54.2% of cell elongation in oat coleoptile as compared with control.

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Development of liquid target for beam-target neutron source & two-channel prototype ITER vacuum ultraviolet spectrometer

  • Ahn, B.N.;Lee, Y.M.;Dang, J.J.;Hwang, Y.S.;Seon, C.R.;Lee, H.G.;Biel, W.;Barnsley, R.;Kim, D.E.;Kim, J.G.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.421-422
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    • 2011
  • The first part is about development of a liquid target for a neutron source, which is designed to overcome many of the limitations of traditional beam-target neutron generators by utilizing a liquid target neutron source. One of the most critical aspects of the beam-target neutron generator is the target integrity under the beam exposure. A liquid target can be a good solution to overcome damage to the target such as target erosion and depletion of hydrogen isotopes in the active layer, especially for the one operating at high neutron fluxes with no need for water cooling. There is no inherent target lifetime for the liquid target neutron generator when used with continuous refreshment of the target surface exposed to the energetic beam. In this work, liquid target containing hydrogen has been developed and tested in vacuum environment. Potentially, liquid targets could allow a point neutron source whose spatial extension is on the order of 1 to $10{\mu}m$. And the second is about the vacuum ultraviolet (VUV) spectrometer which is designed as a five-channel spectral system for ITER main plasma measurement. To develop and verify the design, a two-channel prototype system was fabricated with No. 3 (14.4 nm~31.8 nm) and No. 4 (29.0 nm~60.0 nm) among the five channels. For test of the prototype system, a hollow cathode lamp is used as a light source. The system is composed of a collimating mirror to collect the light from source to slit, and two holographic diffraction gratings with toroidal geometry to diffract and also to collimate the light from the common slit to detectors. The two gratings are positioned at different optical distances and heights as designed. To study the appropriate detector for ITER VUV system, two different electronic detectors of the back-illuminated charge coupled device and the micro-channel plate electron multiplier were installed and the performance has been investigated and compared in the same experimental conditions. The overall system performance was verified by measuring the spectrums.

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Investigation of the Characteristic Velocity of Geomorphologic Instantaneous Unit Hydrograph (지형형태학적 순간단위도의 특성속도에 대한 고찰)

  • Kim, Sang-Dan;Yu, Cheol-Sang;Yun, Yong-Nam
    • Journal of Korea Water Resources Association
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    • v.33 no.3
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    • pp.315-330
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    • 2000
  • The GIUH (Geomorphologic Instantaneous Unit Hydrograph) is to be applied to the ungauged or insufficiently gauged basins. For tIris purpose, an accurate estimation of the charactenstlc velocity is one very important part, but any proper method for this has not been developed yet. In case that we have enough rainfall and runoff clata, the estimation of the characteristic velocity may be an easy job, but it is out of the purpose of the GIUH. Remindmg that the purpose of GIUH the characterisbc veloclty should be estimated based on the geomorpholog1c analysis and also be snnple for easy apphcation. In tIris research analysis cmd application of the GruH was given to several sub-basins in Wi-stream river basin, Gono, Donggok and Hyoryung. After deriving the characteristic velocity througn a optimizatlOn process with real data, it is compared w1th several velOCIties der1ved from geOlnoI1Jhoclimatic instantaneous unit hydrograph theory and several other concentration time formulae. The estimated charactenstic velocities using Kerby, Kim, KInematic Wave, and Brasby- Williams formulae found to g1ve the appropriate results. Hmvever, as the Kerby, and the Kinematic Wave require user's decision of the IvIanning's n value, the K1m and the Braby-Williams seem to be more applicable and recommended as characteristic velocity formula.

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Design and Characterization of a Microwave Plasma Source Using a Rectangular Resonant Cavity (마이크로웨이브 공진 공동을 이용한 플라즈마 원의 설계 및 특성)

  • Kim, H.T.;Park, Y.S.;Sung, C.K.;Yi, J.R.;Hwang, Y.S.
    • Journal of the Korean Vacuum Society
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    • v.17 no.5
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    • pp.408-418
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    • 2008
  • The rectangular resonant cavity was designed and characterized as a microwave plasma source for focused ion beam. The optimum cavity was calculated analytically and analyzed in detail by using HFSS(High Frequency Structure Simulator). Since the resonant cavity can be affected by the permittivity of quartz chamber and plasma, the cavity is designed to be changeable in one direction. By observing the microwave input power at which the breakdown begins, the optimum cavity length for breakdown is measured and compared with the calculated one, showing in good agreement with the optimum length reduced by 10cm according to the permittivity change in the presence of quartz chamber. The shape of breakdown power curve as a function of pressure appears to be similar to Paschen-curve. After breakdown, plasma densities increase with microwave power and the reduced effective permittivity in the cavity with plasma results in larger optimum length. However, it is not possible to optimize the cavity condition for high density plasmas with increased input power, because too high input power causes expansion of density cutoff region where microwave cannot penetrate. For more accurate microwave cavity design to generate high density plasma, plasma column inside and outside the density cutoff region needs to be treated as a conductor or dielectric.

Investigation on the Variation of Discharge Coefficients Following Bifurcation Angle Changes (방수로 분류부 분류각 변화에 따른 유량계수 변화 검토)

  • Rhee, Dong-Sop;Kim, Chang-Wan
    • Proceedings of the Korea Water Resources Association Conference
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    • 2007.05a
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    • pp.926-929
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    • 2007
  • 방수로는 홍수 피해 경감을 사용되는 구조적 대책으로 최근 국내에서 널리 계획되고 있다. 이러한 방수로는 보통 횡월류위어 등으로 대표되는 유입부 구조물을 통하여 하천 본류와 연결되지만, 평상시에도 본류의 유량을 일부 분담하는 형태로 설계되는 젖은 하도(wet channel) 형태의 방수로는 별도의 유입부 구조물을 설치하지 않고 바로 본류에 연결되는 것이 일반적이다. 유입부 구조물을 통해 연결되는 방수로의 특성은 유입부 구조물의 수리학적인 특성에 의해서 많이 좌우된다. 이에 반해 젖은 하도 형태로 본류에서 바로 분기되는 방수로는 그 특성이 방수로의 폭(W), 방수로 분류각$(\theta)$등에 의해서 많이 좌우되며, 만약 설계된 방수로의 횡단면 형상이 사다리꼴 또는 직사각형 단면을 따른다면 이러한 흐름은 "제한 영마루고 횡월류위어 흐름(restricted zero-height side weir flow)"으로 분류할 수 있다. 이러한 조건에서의 흐름은 일반적인 횡월류위어 흐름과 많이 다른 것으로 알려져 있다. 본 연구에서는 이러한 방수로 폭 및 분류각을 변화시켜 방수로 분류 조건을 다양하게 구현하여 실험을 수행하였으며, 특히 분류각 변화에 따른 유량계수의 변화를 분석, 검토하였다. 또한 측정된 방류량을 기준으로 산정된 유량계수를 기존 연구자들이 제시한 영마루고(zero-height) 횡월류위어 유량계수 산정식에 의해 산정된 결과와 비교하여 분석하였다. 분석결과 분류각이 90도인 경우 본류 프루드수의 증가에 따라 유량계수가 점차 감소하였으나, 분류각이 90도 보다 작은 경우에는 본류 프루드수가 증가함에 따라 유량계수가 점차 증가하는 것으로 나타났다. 이러한 한계를 만족시킬 입체 영상) 입체영상은 영화에서 시작되었으나, 20c후반기에 들어서면서 애니메이션 분야와 모바일, 광고 패널, 텔레비전등의 매체를 이용한 입체 영상의 개발로 인하여 특정 분야에 한정 시킬 수 없으므로 영상으로 칭한다. 입체 영상은 21c에 들어서면서 영상매체의 한 분야로 급부상하고 있다. 1900년 무렵부터 연구된 입체영화(3-Dimensional motion Picture)는 In여 년이 지난 지금 대중화를 눈앞에 두고 있다. 국내에서는 놀이 동산이나 박물관등에서 흔히 볼 수 있다. 하지만 앞으로는 HDW등의 대중화로 화질의 발전을 이룬 텔레비전 분야 등에서 실용화 될 전망이다. 국제적인 흐름과 함께 국내에서도 입체 영화에 대한 연구가 활성화 되어 영상산업의 한 주류로서 대두되고 있다. 이러한 상황에서 입체영상에 대한 이해와 콘텐츠(Contents)의 개발은 기술적인 진보에 발맞추어 준비되어야 한다. 본 논문은 이러한 기술적인 계보에 발맞춘 영상 콘텐츠 개발에 박차를 가하고자 앞으로의 발전분야에 대한 기술적인 면과 기법적인 면을 제시하여 기술만 앞서고 내용은 수입하는 수입국이기 보다는 미리 준비하여 비전문가나 타국의 기술에 선점 당하지 않는 분야로 성장할 수 있는 진보적인 영상 인들의 관심과 지속적인 연구를 독려하고자 한다.시민의 휴식 및 여가선용 공간으로 활용하기 위한 사업의 기초자료로 활용되며 이미 설계검토가 시작되었다. 본 연구결과는 유수지 및 저수지의 환경개선 사업의 선두적인 성공사례로 국내 타 지역의 유사한 사업에 있어 벤치마킹을 할 수 있는 훌륭한 사례가 될 것이다.요 생산이 증가하자 군신의 변별(辨別)과 사치를 이유로 강력하게 규제하여 백

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생의학 의용을 위한 마이크로 웨이브 대기압 플라즈마 장비

  • Gang, Seong-Gil;Lee, Jae-Gu
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.259-260
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    • 2011
  • 대기압 플라즈마는 멸균과 살균, 지혈, 피부재생, 치아 미백 등 여러 의학 분야를 대상으로 그 효과를 나타내고 있으며, 플라즈마 장비를 만들어 내기 위해 부피가 큰 진공 장비가 필요하지 않다는 점에서 대기압 플라즈마는 그 활용과 효과에 있어 큰 기대를 받고 있다. 대기압에서 플라즈마는 다양한 주파수를 이용하여 만들어져 왔으며, 본 연구실에서 연구하고 있는 수백 MHz-수 GHz 대역의 파워를 사용하는 플라즈마의 경우 대기압 플라즈마를 의학 분야에 사용할 때 만족해야 할 조건들에 만족하는 특성을 보여준다. 기존의 고주파를 사용하는 장비의 경우 추가적인 Matching 장비로 인해 플라즈마를 만들기 위해 큰 장비와 높은 파워가 필요한 단점이 있었다. 하지만 이 마이크로웨이브 장비는 전송선 이론을 기반으로 장비 자체가 구조적인 Matching이 이루어 지도록 설계되었다(그림 1). 즉, 추가적인 Matching 장비의 필요 없이 외부에서 파워를 주는 것만으로 플라즈마를 발생 시킬 수 있으며, 50% 이상의 파워 효율을 보여준다. 또한 그 크기도 손에 쥐고 사용할 수 있을 볼펜 정도의 크기이며, 3W의 정도의 저 전력으로 플라즈마를 발생 시켰다. 높은 에너지를 가지는 전자들은 공급되는 기체뿐만 아니라, 주변 공기와의 반응하여 여러 응용분야에 적합한 활성 종을 다량 만들어내게 된다. 본 연구실의 강점인 플라즈마 시뮬레이션으로 얻은 결과에서 주파수가 올라 갈수록 높은 에너지를 가지는 전자들이 많아지는 것을 보여준다. 그리고 발생시킨 플라즈마의 광학 특성에서도 생의학 분야에 적합한 많은 활성 종들이 발생 되는 것을 확인하였다. 일반적으로 의학 분야에 사용되는 플라즈마의 경우 플라즈마에서 발생하는 열에 의한 피해를 최소화 하는 것이 중요하다. 마이크로웨이브 플라즈마의 경우, 그 플라즈마의 온도가 50$50^{\circ}$C 미만으로 의학 분야에 사용하기 적합하다. 또한 구동 주파수가 올라갈수록 플라즈마를 유지하는데 필요한 전압이 상대적으로 낮아지게 되는데, 이는 전기적 쇼크 등 플라즈마 의용에서 발생하는 안전성 문제에 있어서도 마이크로 웨이브 장비가 좋은 점이다. 본 플라즈마 장비를 구동하기 위한 손바닥 크기 정도의 소형의 전용 파워 장치를 개발함으로써 저전력 소형 플라즈마 장치를 개발하는 것을 목표로 하고 있다. 마이크로 웨이브 장비는 여러 가지 분야에서 그 효과를 검증 받았다. 혈액 응고 실험에서 30초 정도의 짧은 처리만으로도 자연 응고에 비해 탁월한 지혈 효과를 보여줬다 (그림 2). 충치를 발생시키는 대표적인 구강균인 S.mutans의 살균 실험에서 Ti02와의 복합적인 처리를 통해 30초 미만의 처리로 처리하지 않은 것에 비해 10-6 만큼의 줄어드는 살균 효과를 보여줬다. 뿐만 아니라 치아의 미백에 있어서도 탁월한 효과를 나타냈다. 현재 본연구실에서는 마이크로 웨이브 장비의 기본적인 구조를 응용하여, 좀더 넓은 영역을 처리할 수 있는 대면적 마이크로 웨이브 장비를 위한 연구를 수행 중이다.

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A Study of the Photoluminescence of ZnO Thin Films Deposited by Radical Beam Assisted Molecular Beam Epitaxy (라디칼 빔 보조 분자선 증착법 (Radical Beam Assisted Molecular Beam Epitaxy) 법에 의해 성장된 ZnO 박막의 발광 특성에 관한 연구)

  • Suh, Hyo-Won;Byun, Dong-jin;Choi, Won-Kook
    • Korean Journal of Materials Research
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    • v.13 no.6
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    • pp.347-351
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    • 2003
  • II-Ⅵ ZnO compound semiconductor thin films were grown on $\alpha$-Al$_2$O$_3$(0001) single crystal substrate by radical beam assisted molecular beam epitaxy and the optical properties were investigated. Zn(6N) was evaporated using Knudsen cell and O radical was assisted at the partial pressure of 1$\times$10$^{4}$ Torr and radical beam source of 250-450 W RF power. In $\theta$-2$\theta$ x-ray diffraction analysis, ZnO thin film with 500 nm thickness showed only ZnO(0002)and ZnO(0004) peaks is believed to be well grown along c-axis orientation. Photoluminescence (PL) measurement using He-Cd ($\lambda$=325 nm) laser is obtained in the temperature range of 9 K-300 K. At 9 K and 300 K, only near band edge (NBE) is observed and the FWHM's of PL peak of the ZnO deposited at 450 RF power are 45 meV and 145 meV respectively. From no observation of any weak deep level peak even at room temperature PL, the ZnO grains are regarded to contain very low defect density and impurity to cause the deep-level defects. The peak position of free exciton showed slightly red-shift as temperature was increased, and from this result the binding energy of free exciton can be experimentally determined as much as $58\pm$0.5 meV, which is very closed to that of ZnO bulk. By van der Pauw 4-point probe measurement, the grown ZnO is proved to be n-type with the electron concentration($n_{e}$ ) $1.69$\times$10^{18}$$cm^3$, mobility($\mu$) $-12.3\textrm{cm}^2$/Vㆍs, and resistivity($\rho$) 0.30 $\Omega$$\cdot$cm.

Immunohistochemical Study on the TNFα-Secreting Macrophages and Endothelial Cells in the Porcine Corpus Luteum (돼지 황체에서 TNFα-분비 대식세포와 내피세포에 관한 면역조직화학적 연구)

  • Park, C.S.;Han, S.R.;Kim, S.I.;Cho, K.J.;Kim, W.S.
    • Journal of Animal Science and Technology
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    • v.47 no.5
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    • pp.711-720
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    • 2005
  • In the corpus luteum, TNF$\alpha$ is known to induce functional and structural luteolysis. In addition, it acts as luteotropic agent during the initial and early stage of luteal development. In spite of its importance in corpus luteal development, there is still different opinions for the source cells of TNF$\alpha$ in the corpus luteum. One is the macrophages only, and the other is macrophages are the main source and endothelial cells are the minor source. In this experiment, using the porcine corpora lutea of pregnancy and ovulatory stages, hematoxylin-eosin stain, macrophage and TNF$\alpha$ immunohistochemistry were carried to reveal the sources of TNF$\alpha$. As a result, MAC 387-positive macrophages were present in all the stages of corpora lutea. In the mature corpora lutea of nonpregnant stages, the sites of MAC 387-positive macrophages and those of TNF$\alpha$- positive macrophages were coincided, and the sites of endothelial cells and those of TNF$\alpha$-positive endothelial cells were nearly coincided. But, in the mature CL of pregnant stage, mid- and advanced luteolytic stages of both nonpregnant and pregnant stages, the sites of MAC 387-positive macrophages and those of TNF$\alpha$-positive macrophages were coincided, but not in the endothelial cells. Accordingly, it can be concluded that macrophages are the main source of TNF$\alpha$ in the corpus luteum and endothelial cells are the minor source in the mature and mid-lytic stages, but, in the advanced luteolytic stage, macrophages are the only source of TNF$\alpha$.

A Study on Estimation of Individual Growth Curve Parameters and their Relationships with Meat Quality Traits of Crossbred between Korean Native Boars and Landrace Sows (재래돼지와 랜드레이스 교잡종의 개체별 성장곡선 추정 및 육질형질과의 상관관계 추정에 관한 연구)

  • Cho, Y.M.;Choi, B.H.;Kim, T.H.;Lee,, J.W.;Lee, J.E.;Oh, S.J.;Cheong, I.C.
    • Journal of Animal Science and Technology
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    • v.46 no.4
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    • pp.503-508
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    • 2004
  • This study was conducted to estimate the growth curve parameters of crossbreds between Korean native boars and Landrace sows and their relationships with meat qualities. The data used were weight-age data and carcass data from 131 males and 122 females raised at N.L.R.I in Korea. Growth curve parameters were estimated from nonlinear regression using Gompertz model individually. Average mature weight(A), average maturing rate(k), and average inflection point(u) showing maximum growth rate estimated were 179.54${\pm}$6.06kg, 0.3154${\pm}$0.0059, and 5.50${\pm}$0.11 months in females, and 179.84${\pm}$6.33kg, 0.3049${\pm}$0.0061, and 5.24${\pm}$0.13 months in males, respectively. For the growth curve parameters and derived statistics, the phenotypic correlations of maturing rate with gain rate at inflection, mature weight, and inflection point were - .30, - .77, and - .93 in male, and - .31, - .78 and - .94 in female, respectively. Matrure weight was positively correlated to the inflection point as + .89 in both male and female, indicating that late maturing pigs with lower k had longer maturing period with increasing gain rate and reached point of inflection later than early maturing pigs with higher k, and grew to larger mature weight. Backfat thickness and erode fat contents were correlated with mature weight positively in male and negatively in female, and correlated with gain rate at inflection point positively in both male and female, of which coefficients were as high as .42 and .50 in male, respectively.

Inhibition of Benzo[a]pyrene-Induced Mouse Forestomach Neoplasia by Astaxanthin-Containing Egg Yolks (Benzo[a]pyrene으로 유발한 Mouse Forestomach Tumor 생성에 대한 Astaxanthin 함유 난황의 효과)

  • Lee, Sang-H.;Park, Cherl-W.;Park, Won-S.;Lee, Young-C.;Choi, Eui-S.;Ha, Yeong-L.
    • Applied Biological Chemistry
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    • v.40 no.6
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    • pp.490-494
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    • 1997
  • Anticarcinogenic activity of astaxanthin-containing egg yolks (designate AEY) was investigated for benzo[a]pyrene (BP)-induced mouse forestomach tumorigenesis initiating regimen. Female ICR mouse (6-7 weeks of age) were housed in polycarbonated cages (5 mice/cage; 20 mice/treatment) in a humidity-and-temperature-controlled facility and permitted free access to water and food. One week later, four and 2 days prior to p.o. treatment with BP (2 mg/0.2 ml corn oil), mice were given 0.2 ml PBS containing 50 mg AEY, 100 mg AEY, 150 mg AEY, or 150 mg CEY. Control mice were only given 0.2 ml PBS. Three days later this sequence was repeated for a total of 4 times. Beginning with the first intubation and continuing thereafter, body weight and food intake were recorded once weekly. All surviving mice were sacrificed 24 weeks after the first dose of BP. Mice treated with AEY developed only about one third as many neoplasms/animal as mice in control or CEY-treated group (p<0.05). Reduction effect of tumor development by AEY was dependent upon doses applied. Tumor incidence was also reduced by AEY treatments, but significantly reduced only by 150 mg AEY treatment when compared to that by control or CEY. Food intake and body weight were not affected by AEY treatment. These results indicate that AEY inhibits tumorigenesis of mouse forestomach induced by BP.

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