• Journal of Microbiology and Biotechnology
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• 제21권6호
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• pp.617-626
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• 2011

Transglutaminase from Streptomyces mobaraensis is an enzyme of unknown function that cross-links proteins to high molecular weight aggregates. Previously, we characterized two intrinsic transglutaminase substrates with inactivating activities against subtilisin and dispase. This report now describes a novel substrate that inhibits papain, bromelain, and trypsin. Papain was the most sensitive protease; thus, the protein was designated Streptomyces papain inhibitor (SPI). To avoid transglutaminase-mediated glutamine deamidation during culture, SPI was produced by Streptomyces mobaraensis at various growth temperatures. The best results were achieved by culturing for 30-50 h at $42^{\circ}C$, which yielded high SPI concentrations and negligibly small amounts of mature transglutaminase. Transglutaminasespecific biotinylation displayed largely unmodified glutamine and lysine residues. In contrast, purified SPI from the $28^{\circ}C$ culture lost the potential to be cross-linked, but exhibited higher inhibitory activity as indicated by a significantly lower $K_i$ (60 nM vs. 140 nM). Despite similarities in molecular mass (12 kDa) and high thermostability, SPI exhibits clear differences in comparison with all members of the wellknown family of Streptomyces subtilisin inhibitors. The neutral protein (pI of 7.3) shares sequence homology with a putative protein from Streptomyces lavendulae, whose conformation is most likely stabilized by two disulfide bridges. However, cysteine residues are not localized in the typical regions of subtilisin inhibitors. SPI and the formerly characterized dispase-inactivating substrate are unique proteins of distinct Streptomycetes such as Streptomyces mobaraensis. Along with the subtilisin inhibitory protein, they could play a crucial role in the defense of vulnerable protein layers that are solidified by transglutaminase.

• 여성건강간호학회지
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• 제26권1호
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• pp.93-103
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• 2020

Purpose: This study was conducted to identify unmet healthcare needs among man and woman one-person households and to explore related factors by gender. Methods: Data were drawn from the 2017 Korea National Health and Nutrition Examination Survey. The final sample consisted of 820 one-person households. The statistical analysis, conducted in SPSS version 20.1, included complex sampling analysis; descriptive statistics, the chi-square test, and logistic regression. Results: The demographic and socioeconomic characteristics of women in one-person households were significantly different from those of men in one-person households. Women in single-person households were mainly in their 70s or older and married, and they tended to have a low education level, low income, and no formal occupation. Unmet healthcare needs were experienced by 17.3% of women in one-person households and 13.5% of men in one-person households, which was not a statistically significant difference (χ²=2.17, p=.139). Factors related to unmet healthcare needs were subjective health status and unmet dental care needs in single-person-household men. By contrast, having experienced impairment within the past year, stress, and unmet dental care needs were factors related to unmet healthcare needs in single-person-household women. Conclusion: As one-person households become increasingly common, more attention needs to be paid to them and our understanding of them needs to be improved. Women in one-person households, in particular, are especially vulnerable, as they experience more unmet healthcare needs.

• 성인간호학회지
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• 제15권3호
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• pp.452-462
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• 2003

Purpose: The purpose of this study is to identify the effects of telephonic counseling on burnout, depression, life satisfaction, and perceived physical health among family caregivers of older adults with dementia. Method: Subjects were randomly assigned into telephonic counseling group (n=21) and the comparison group (n=32). A weekly telephone counseling was conducted by research assistants for 12 weeks. T-test were used to answer the research questions. Result: 1) There were no significant differences between the two groups on the level of burnout, depression, life satisfaction, and perceived physical health after telephone counseling. 2) Spouse caregivers under the telephone counseling tended to report higher perceived physical health than comparison group at the post-test (t=-1.88, p=.08). Spouse caregivers under the telephone counseling tended to report higher emotional exhaustion and lower feeling of self achievement. 3) Daughter-in-law caregivers under telephone counseling showed increased feeling of self achievement, improved physical health condition, and decreased depression. Conclusion: This study showed potential effects of the problem-solving telephone counseling to improve perceived physical health and to reduce the level of burnout and depression. The findings suggest the necessity of screening most vulnerable subgroups of caregivers to increase the effectiveness of nursing intervention such as telephone counseling.

• 융합보안논문지
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• 제16권7호
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• pp.139-145
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• 2016

최근 국내 여러 기반 시설에 USB와 네트워크에 대한 공격이 증가하고 있다. 이러한 공격은 대부분 내부자에 의한 것으로 의도와는 달리, 인위적인 조작에 의해 발생하기도 한다. 이에 따른 인위적 공격의 대응 방안으로 물리보안을 꼽아볼 수 있으며, 대표적인 기술로는 CCTV나 출입통제시스템, 센서 기술이 있다. 그러나 물리보안은 시장성에 따라 몇몇 제품군으로 대표되고 있어, 다양한 물리보안의 기술개발과 확대 적용에 오히려 걸림돌이 되고 있다. 이러한 상황에서 네트워크의 인위적 취약요인으로 인한, 공격이 지속적으로 발생함에 따라, 적절한 물리적 대응기술이 필요한 실정이다. 따라서 본 논문은 물리보안의 인지도와 수요 동향을 알아보고, 네트워크의 물리적 취약요인과 이에 따른 대응기술들을 알아봄으로써, 향후, 국내 물리 보안기술 개발 및 로드맵 구축에 기여할 것으로 기대한다.

• 한국컴퓨터정보학회논문지
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• 제16권12호
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• pp.265-271
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• 2011

고품질의 맞춤형 서비스는 사회가 고령화, 초핵가족화, 재난 취약 계층의 증가로 인하여 급격히 늘어나게 되었다. 급성 질환, 심뇌혈관 질환, 자살 등 예방 가능한 사망률이 선진국에 비해 높으므로 빠르고 전문적인 구조 및 구급 서비스가 요구된다. 본 논문에서는 응급 환자가 발생하여 병원에 도착하기 전에 환자의 정보를 이용하여 응급처치가 가능하도록 한다. 그리고 응급 의료 기관에서는 환자 진료 준비를 사전에 갖출 수 있도록 하여 응급 환자 진료의 효율성을 높였다. 사회적 인지도가 높은 119번호를 이용하여 다양한 복합 응급 신고를 접수하고 있고, 유관기관과 통합적 대응 체계를 구축하는 효율적 응급의료서비스 선진화 전략을 제시하고자 한다.

• Child Health Nursing Research
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• 제24권2호
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• pp.148-156
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• 2018

Purpose: The aim of this study was to evaluate the influence of the mothers' native country on influenza vaccinations in adolescents in multicultural families. Methods: Data were gathered from the 13th (2017) Youth Risk Behavior Web-Based Survey. Logistic regression analyses were conducted using a complex sample data analysis method. The participants in this study had a father who was born in Korea and a mother born outside of Korea. The sample included 481 adolescents. Results: The analysis of non-adjusted confounding variables showed that influenza vaccination was higher in multicultural adolescents whose mother's native country had an annual minimum temperature less than $21^{\circ}C$ (odds ratio [OR]: 1.81, 95% confidence interval [CI]: 1.20~2.74). Furthermore, when the analysis was adjusted for confounding variables, an annual minimum temperature less than $21^{\circ}C$ in the mother's native country had a statistically significant association with influenza vaccination (OR: 2.12, 95% CI: 1.36~3.29). Conclusion: Multicultural adolescents belong to a socioeconomically vulnerable class, and their health promotion behaviors are influenced by their mothers' culture. Thus, healthcare providers and school nurses should provide adolescents with appropriate information related to influenza vaccination depending on their mothers' culture and their family's cultural background.

• 한국직업건강간호학회지
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• 제25권4호
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• pp.362-372
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• 2016

Purpose: This study intended to grasp real context of Cardiovascular disease (CVD)-related factors of Korean blue-collar workers, especially CVD-related knowledge, perception, beliefs, benefits and barriers of behaviors according to the health belief model. Methods: We interviewed twenty two workers working in two small-sized companies and performed two series of focus group interviews. Data were analyzed by deductive content analysis approach based on Elo & $Kyng{\ddot{a}}s$. Results: Excepting participants who have CVD risk factors, most participants had lower level of CVD risk perception. The level of CVD knowledge was low but there was difference by gender. CVD-related beliefs were 'fatal disease', 'caused by lifestyle' and 'difficult to prevent by themselves'. The risk reduction behaviors were motivated by current or family history of hypertension. But there were barriers to interfere practice of preventive behaviors such as poor quality of food provided by cafeteria in the workplace, frequent overtime, victim mentality as one of vulnerable social group, housework and financial burden, lack of facilities for rest and physical activity in the workplace. Conclusion: To develop intervention for reducing CVD risks in Korean blue-collar workers, we need to focus on improving CVD knowledge and perception and modifying work-related environments such as low quality of food and lack of facilities for rest and physical activity in the workplace.

• Child Health Nursing Research
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• 제23권2호
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• pp.158-167
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• 2017

Purpose: The purpose of this study was to investigate the ecological factors influencing school adjustment of adolescents from low-income families. Methods: Secondary data analysis was performed using data of 1,321 low-income adolescents in 123 regions found on the Survey on Service Satisfaction with Community Child Care Center. Results: The results of multi-level analysis identified the factors influencing school adjustment of low-income adolescents as follows: individual-level factors were gender, grade in school, and emotional problem; an interpersonal-level factor was family structure; organizational-level factors were length of time attending center and satisfaction with the service of the center; community-level factors were region and perception of community. Conclusion: The results suggest that low-income adolescents' adjustment to school is influenced not only by individual factors but also by diverse environmental factors. Community factors suggest that more education support systems and leisure facilities for adolescents need to be built in small and medium cities. Strategies to enhance positive perception of community are also needed for this population. Further, it is necessary to develop multi-level interventions to improve the school adjustment of adolescents from vulnerable social groups.

• Safety and Health at Work
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• 제11권1호
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• pp.19-25
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• 2020

Background: Childbirth represents a significant transition for women, with physical and psychological sequelae. Reentry to the workplace during the postpartum period is understudied, with implications for maternal well-being and job-related outcomes. This study's aim was to examine selected pregnancy, childbirth, and return-to-work correlates of overall self-rated health within the first month of work reentry after maternity leave. Methods: Between December 2016 and January 2017, we surveyed women employed at a large, public Midwestern university who had given birth in the past five years (N = 249) to examine self-rated overall health in the first month of work reentry. Using ordinal logistic regression, we examined whether physical or psychological health problems during pregnancy, childbirth complications, length of maternity leave, and depression and anxiety at work reentry were related to overall health. Results: Women who experienced depression (odds ratio [OR] = 0.096 [95% confidence interval {CI} = 0.019 to 0.483, p = 0.004]) and anxiety (OR = 0.164, [95% CI = 0.042 to 0.635, p = 0.009]) nearly every day reported worse health at work reentry than those with no symptoms. Controlling for demographics and mental health, women who experienced medical problems during pregnancy (OR = 0.540 [95% CI = 0.311 to .935, p = 0.028]) were more likely to report poor health, while taking a longer maternity leave (OR = 14.552 [95% CI = 4.934 to 42.918, p < 0.001]) was associated with reporting better health at work reentry. Conclusion: Women who experience medical complications during pregnancy, return to the workplace too soon after birth, and experience mental health symptoms are vulnerable physically as they return to work.

• 대한가정학회지
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• 제34권4호
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• pp.119-131
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• 1996

The relationship of maternal separation anxiety, availability of support system and child gender to initial preschool adjustment was examined. Data were collected on 72 middle-class Korean children(35 boys, 37 girls) between the ages of 31 months and 60 months who were entering preschool. Subjects were from intact families, all with non-working mothers. Mothers completed the Maternal Separation Anxiety Scale(MSAS) and indicated the number of extended family members in the home as an index of social support. Classroom teachers completed the Preschool Behavior Questionnaire (PBQ) for each child after the first 2 weeks and again after 6 weeks of school. Each child was observed by the research team during the first 2 weeks of school and again after the 6th-week using the Child Observation System (COS). Applying the COS observed behavior was classified as being in the dependent, neutral, or active mode. All instruments were culturally relevant as validated by Korean experts. Maternal Separation Anxiety was a significant predictor for girls only, accounting for 11% of initial COS variance (p=＜.05). In contrast, social support was a significant predictor for boys only, accounting for 28% of initial PBQ variance when entered after MSAS(p=.005). A significant difference between initial and 6th-week adjustment was obtained in the expected direction on the COS with behavior improving with time (t=-4.06, p=.001). Although a significant difference between initial and 6th-week PBQ adjustment was also obtained (t=-3.63, p=.001), it was not in the direction expected, problematic behavior were found to increase with time. Cultural and developmental factors help explain the findings. Given the patrilineal social system in Korea, boys are highly preferred over girls. Thus the at-home support system, comprised primarily of grandparents, may have a significant influence on school adjustment particularly of first-born sons. In contrast, since girls are viewed as fragile and vulnerable, mothers may express greater separation anxiety about girls. Finally, as would be expected, older children may be able to develop coping skills more readily than younger ones, as child adjustment on the COS significanlty improved over the 6-week period.