• Title/Summary/Keyword: Vt. E

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The Degree of Lipid Oxidation of Rat Liver Fed Peroxidized Lipid and Its Effects on Anti-Oxidative System (과산화지질의 투여가 흰쥐 간의 산화와 항산화계에 미치는 영향)

  • 권명자;전영수;송영옥
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.6
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    • pp.899-907
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    • 1994
  • Accumulation of peroxidized lipid, fed or injected in the body of rats was investigated and the effect of peroxidized lipid on the antioxidative system was studied also. Three groups each having six of Sprague-dawley rats were raised for 8 weeks. the peroxide value(POV) of diet fed to the control and the peroxidized group was 5.47 and 22.14meq/kg , respectively. Injected group was given the control diet and peroxidized linoleic acid(POV 31.81meq/kg) was injected into the peritoneal area three times a week. The POV, MDA, and protein carbonyl values of the peroxidized and the injected group (experimental groups) were significantly higher (p<0.05) than those of the control group. Cu, Zn-SOD and M-SOD activity of the experimentla groups increased 1.6 times that of control group at 4 th week. and decreased by 60% of their activityafter 8 weeks of feeding (p<0.05) . Catalase activity, glutathione and Vt, E contents of the experimental groups were significantly lower (p<0.05) than those of the control group during 8 weeks. The accumulation of peroxidcized lipid in liver were ovserved both in the fed or the injected group. The increased of enzyme activity of the experimental group during 4 weeks suggests ianadaptation of antioxidative system to get rid of the peroxidized lipid. Decrease of enzyme activity and glutathione observed as the peroxidized lipid lipid accumulation proceeded further, however, seems to indicate the oxdiative damage of enzyme and protein . Determination of the protein carbonyl content may be used as a method for measuring the oxidative damaging effect of peroxidized lipid.

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Transfer of Genes for Antimicrobial Resistance and Toxin of Hemolytic Escherichia coli Isolated from Feces of Pig Suffering Diarrhea to Human Isolates (설사 증상의 돼지 분변에서 분리된 용혈성 대장균의 항생제 내성과 독소의 인체로부터 분리된 균주로의 전이)

  • Lee Kyenam;Jung Byeong Yeal;Lee Yeonhee
    • Korean Journal of Microbiology
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    • v.40 no.4
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    • pp.286-294
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    • 2004
  • Between 1997 and 1998 in Korea, 56 isolates of Escherichia coli were obtained from pig suffering diarrhea. Among those, 38 isolates that showed the hemolytic activity, antimicrobial resistance, and toxin production were studied. Among 38 isolates, thirty-six isolates $(94.7\%)$ were resistant to tetracycline, 27 isolates $(71.0\%)$ were resistant to ampicillin, 26 isolates $(68.4\%)$ were resistant to chloramphenicol, and 21 isolates $(55.2\%)$ were resistant to trimethoprim, while none was resistant to aztreonam, amikacin, and norfloxacin. Among these iso­lates, 21 isolates $(55.3\%)$ were multiple drug resistant to at least four different class antimicrobial agents. Extended spectrum $\beta-lactamase$ producing isolates were not detected in the double disk synergy test. In these hemolytic Escherichia coli, heat-stable enterotoxin $(89.5\%)$ was the most prevalent toxin, followed by vero­toxins $(47.4\%),$ and then heat-labile enterotoxin $(31.6\%).$ Except 8 isolates $(21.0\%)$ which produced ST only, 12 isolates $(31.6\%)$ produced ST and LT, 13 isolates $(34.2\%)$ produced ST, VT, and VTe, and 5 isolates $(13.2\%)$ produced VT and VTe. However, none produced all 4 types of toxin, simultaneously. The predominant serotype could not be determined by the agglutination method. Sixteen isolates $(42.1\%)$ were strongly adhered to T-24 bladder cell and 17 isolates $(44.7\%)$ were to Caco-2 intestinal cell. Especially, 11 strains $(28.9\%)$ were evaluated as strongly adhesive to both T-24 cells and Caco-2 cells. Genes for toxin and the antimicrobial resistance were transferred to clinical isolates of Escherichia coli from human urine by the filter mating method. Results suggest the possibility that antimicrobial resistance and toxin can be transferred from animals to humans by direct con­tact of resistant bacteria as well as gene transfer, although there was no correlation between toxin production, adherent activity, and antimicrobial resistance among hemolytic E. coli isolated from pig suffering diarrhea.

Formant Transition Shapes of Korean Front Vowels (한국어 전설 모음의 포먼트 전이 형태)

  • Oh, Eunjin
    • Phonetics and Speech Sciences
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    • v.5 no.4
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    • pp.195-200
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    • 2013
  • This study investigates formant transition shapes of Korean front vowels produced by native speakers of Seoul Korean. Sixteen speakers (eight male and eight female speakers) produced [pVt] syllables where the vowels were [i, e, ɛ]. F1, F2, and F3 transition shapes were estimated by presenting formant values at 11 points by dividing the vowel duration into 10 different time intervals. The results indicated that the male and female speakers overall demonstrated similar formant transition shapes and measurement points arriving at the maximum and minimum formant values for the three front vowels. As for the vowels [e] and [ɛ], both male and female speakers showed similar formant values across the 11 measurement points and similar measurement points arriving at the maximum and minimum values, indicating that the two Korean vowels have been merged not only in the steady-state formant values, but also in the dynamic transition shapes.

OBSERVATIONS ON A FURTHER IMPROVED ($\frac{G}{G}$) - EXPANSION METHOD AND THE EXTENDED TANH-METHOD FOR FINDING EXACT SOLUTIONS OF NONLINEAR PDES

  • Zayed, E.M.E.
    • Journal of applied mathematics & informatics
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    • v.30 no.1_2
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    • pp.253-264
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    • 2012
  • In the present article, we construct the exact traveling wave solutions of nonlinear PDEs in the mathematical physics via the (1+1)-dimensional Boussinesq equation by using the following two methods: (i) A further improved ($\frac{G}{G}$) - expansion method, where $G=G({\xi})$ satisfies the auxiliary ordinary differential equation $[G^{\prime}({\xi})]^2=aG^2({\xi})+bG^4({\xi})+cG^6({\xi})$, where ${\xi}=x-Vt$ while $a$, $b$, $c$ and $V$ are constants. (ii) The well known extended tanh-function method. We show that some of the exact solutions obtained by these two methods are equivalent. Note that the first method (i) has not been used by anyone before which gives more exact solutions than the second method (ii).

A Pilotage Information Service of VTS for PortCDM (PortCDM을 위한 도선 스케줄 정보 기반 VTS에서의 Pilotage 정보제공 서비스)

  • Lee, Byung-Gil;Na, Jung-Chan
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2019.05a
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    • pp.40-41
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    • 2019
  • In the VTS, the predictions of vessel mobility and situation awareness of maritime environment are basic function. In recent years, pilotage information is an essential aware element of VTS personnel for vessel traffic management. So, we designed the structure of pilotage information service with VTS and tested in real environment. In the future, similar pilotage information can be used as a useful VTS service.

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A Study on the Effective VTS Communications Analysis by the Method of VCDF in Busan Port (VCDF 방식을 통한 효율적인 VTS 통신 데이터 분석에 관한 연구 - 부산항을 대상으로 -)

  • Kim, Bong-Hyun;Park, Young-Soo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.22 no.4
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    • pp.311-318
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    • 2016
  • The VTS concept was located as a principal methods of maritime safety administration in world's major harbors and expected to become the pivotal role for the future of the maritime and harbor society with e-Navigation epoch. If recent limelight concept of big-data has been included in aspect of information gathering and analysis with various studies, it's required advanced studies to improve the information analysis capability and application range of the data that can be mining by the VTS. In this study, contrast to other studies that aimed quantitative analysis as communication number, it can be mining the time information and each of the communication VTS for the target vessel, including qualitative analysis, such as the purpose or the type of communication. This comparison across multiple items of the collected information, and presenting the VTS data mining model (VCDF) that can be analyzed for the purpose of analyzing way, type and number of communication by ship's type, also number of violations through VTS communication. First, In Busan port case, it shows frequently information service and shows frequently communicating with particular types of vessels. Second, Passive VTS carried out notwithstanding many kinds of traffic violations due to communication congestion. This arranged information can be used as data for the analysis, as possible the level of traffic for VTSO situational awareness, which pointed to the 'workloads' in 'IALA Guideline' and could be used as a database for future research of e-Navigation.

Seasonal Changes of Water Relations Parameters of the Korean Mistletoe (Viscum album var. coloratum) Leaves (겨우살이 엽의 계절별 수분특성)

  • Lee, Kyeong-Cheol;Kim, Cheol-Woo;Yi, Jae-Seon;Han, Sang-Sup
    • Journal of Korean Society of Forest Science
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    • v.101 no.3
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    • pp.461-468
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    • 2012
  • This study was conducted to study the seasonal changes of relative water relations parameters by pressure-volume curves of Korean mistletoe (Viscum album var. coloratum) leaves for understanding ecological characteristics. In growing season, the osmotic potentials at full turgor (${\Psi}_o^{sat}$) and at incipient plasmolysis (${\Psi}_o^{tlp}$) decreased, while increased the maximum bulk elastic modulus of the cell wall ($E_{max}$) and relavive water content ($RWC^{tlp}$). Korean mistletoe in Quercus variabilis and Korean mistletoe in Quercus mongolica in November showed best maximum perssure potential (${\Psi}_{P,\;max}$). Pressure potential (${\Psi}_P$) and water potential (${\Psi}_L$) in Korean mistletoe in Quercus variabilis were rapidly decreased with decreasing of relative water content. The values of $RWC^{tlp}$ in November were all above 84% showing that the function of osmoregulation is somewhat better, and symplastic water content (Vo/DW) and maximum water content (Vt/DW) were variable seasonally. Thus, responses to water relations of Korean mistletoe in Quercus variabilis and Korean mistletoe in Quercus mongolica such as ${\Psi}_o^{sat},\;{\Psi}_o^{tlp},\;E_{max},\;{\Psi}_{P,\;max},\;RWC^{tlp}$ showed increaing drought tolerance with increasing of leaf aging.

Characterization and isolation of shiga toxin-producing Escherichia coli from Bovine feces and Carcass (소의 분변과 도체에서 shiga toxin-producing Escherichia coli의 분리와 특성)

  • Chae, Hee-Sun;Kim, Neung-Hee;Han, Hye-Jin;Son, Hong-Rak;Kim, Chang-Ki;Kim, Sun-Heung;Lee, Jung-Hark;Kim, Jong-Taek
    • Korean Journal of Veterinary Service
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    • v.32 no.3
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    • pp.241-249
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    • 2009
  • Shiga toxin (Stx)-producing Escherichia coli (STEC) strains can cause broad spectrum of human disease, including diarrhea, hemorrhagic colitis, and the life-threatening hemolytic uremic colitis (HUS). We examined 868 samples was taken from bovine feces and carcass from January to December 2008 in Seoul. Twenty two (9.5%) shiga toxin -producing Escherichia coli were isolated from the 230 of bovine feces, and two (0.31%) were isolated from the 638 of carcasses. Serotype of E. coli isolates were O157 (10, 41.6%), O26 (10, 41.6%), O111 (1, 4.2%) and UT (3, 12.6%). In PCR, the isolates displayed three different stx gene combination (stx1 [2, 8.4%]), stx2 [3, 12.6%] and stx1 and stx2 [19,87.5%]). The eaeA and hlyA gene were found in 11 (45.8%) of the 24 strains. Saa gene was present only one strains (4.2%). Toxin typing using reverse passive latex agglutination test showed the same result in VT 1. But it showed different result in VT 2. In antimicrobial susceptibility test, all isolates were sensitive to amikacin, amoxicillin/clavulanic acid, ciprofloxacin and colistin. Eighteen strains (75.0%) of 24 isolates showed the multi-resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty four isolates was digested with Xba I. the 24 isolates showed 7 (A~G) PFGE type.

Virulence Profile and Antimicrobial Resistance of Escherichia coli from Flies Captured from Agricultural Environment (농업환경에 서식하는 파리에서 분리된 E. coli의 병원성 유전자 및 항생제 내성 조사)

  • Yun, Bohyun;Jang, Youn Jung;Kim, Yeon Rok;Kim, Hwang-Yong;Kim, Won-Il;Han, Sanghyun;Kim, Se-Ri;Ryu, Jae-Gee;Kim, Hyun Ju
    • Journal of Food Hygiene and Safety
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    • v.32 no.2
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    • pp.147-153
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    • 2017
  • The purpose of this study was to isolate Escherichia coli from flies and to assess pathogenic genes and antibiotic resistance of the isolates. A total of 188 flies were captured in agricultural environment including fruits farms (n = 19), fermented soybean farms (n = 9), municipal waste (n = 46), livestock farms (n = 66), slaughterhouses (n = 38), and manure ground (n = 10). E. coli isolates of captured flies were tested for pathogenic gene and antibiotic resistance using PCR methods and VITEK2 systems. As a result, E. coli from 63% (119/188) of the captured flies has been detected, and the detection rate of E. coli was the highest (89%, 31/34) in flies captured at particular slaughterhouse. Of the 34 isolates, 94% (32/34) were pathogenic gene (ST gene) positive. Twenty-six percent (31/119) of the E. coli isolates were observed being resistant to one or more antibiotics. Markedly, one of E. coli isolates from Livestock farms was resistant to 7 antibiotics including ampicillin, ampicillin/sulbactam, cefazolin, cefotaxime, gentamicin, levofloxacin, and trimethoprim/sulfamethoxazole. In addition, it was ESBL positive. The results of the present study may suggest a risk of transmission of pathogenic and antimicrobial resistant bacteria from flies to livestock environment Therefore, it may need to prevent introducing flies into the agricultural production environment for safe food production.

Conceptual Design of the Decision-Making Support System for VTS Operator (관제사 의사결정 지원 시스템 개념 설계)

  • Kim, Sun-Young;Oh, Jae-Yong;Kim, He-Jin;Son, Nam-Sun;Lee, Moon-Jin
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2010.10a
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    • pp.144-145
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    • 2010
  • VTM is regarded as a core element in e-Navigation under developing by IMO. IMO's e-Navigation aims for VTM which could provide more navigational information for ships and provide more active VTS services for safe and efficient marine traffic environment. To be successful for VTM under e-Navigation, VTS operator's experience and ability for carrying out VTM tasks are essential. In this study, Decision-making support system for VTS operators are designed conceptually which support VTS operators' VTM tasks by providing informations on traffic risk, and predicted behaviour of ships. This paper describes a designed system with design concept, main functions, main elements and necessary technologies to be developed.

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