• Title/Summary/Keyword: Vitis

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Changes of Volatile Flavor Compounds of Seibel Grape Must during Alcohol Fermentation and Aging (Seibel 포도즙 알코올 발효 및 저장 중 휘발성 향기성분의 변화)

  • 고경희;장우영
    • Microbiology and Biotechnology Letters
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    • v.27 no.6
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    • pp.491-499
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    • 1999
  • A great variety of the volatile metabolic by-products was formed in yeast cell during alcohol fermentation. The seibel grape (Vitis labrasca) which was grown in the Southern Korea used for wines. The objective of this research was to identify the volatile flavor compounds during alcohol fermentation and aging at 12$^{\circ}C$. saccharomyces cerevisiae and Schizosaccharomyces pombe were inoculated and fermented in seibel grape must. The volatile flavor compounds of logarithmic, stationary and death phases were extracted, concentrated and identified by gas chromatography/mass spectrometer (GC/MS). The volatile flavor compounds were determined by a Hewlett-Packard 5890 II Plus GC which was equipped with Supelcowax 10 fused silica capillary column (60m$\times$0.32mm$\times$0.25${\mu}{\textrm}{m}$ film thickness) wall coated with polyethyleneglycerol. The scan detection method allowed the comparison of the spectrum from the chromatogram of volatile flavor compounds to those in data Wileynbs base library. Among the volatile compounds collected by ether-hexane extraction method, the evolution of 20 main compounds, such as 9 esters (ethyl butyrate, isoamyl acetate, ethyl caproate, n-hexyl acetate, ethl caprylate, ethyl caprate, diethy succinate, ethyl hexadecanoate, 2-pheneethyl acetate), 4 alcohols (3-methyl-1-butanol, 1-hexanol, 1-heptanol, benzoethanol), 4 ketones and acids (2-octanone, caproic acid, caprylic acid, capric acid), 2 furan and phenol (2,6-bis(1,1-dimethyl ethyl)phenol, 2,3-dihydrobenzofuran) were observed during alcohol fermentation and aging. The production of the esters during alcohol fermentation with S. cerevisiae was higher than those of Sch. pombe. The sensory scores of the aged wine samples in aroma, taste and overall acceptability were not significantly different(p<0.05).

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Effects of Soil Moisture Control and Dormancy Breaking Agents on Bud Burst and Fruiting for Double Cropping System in a Year in 'Kyoho' Grapes (포도 '거봉' 2기작재배를 위한 하계휴면타파에서 토양수분 조절과 휴면타파제 처리가 발아에 미치는 영향)

  • 오성도;김용현;최동근
    • Journal of Bio-Environment Control
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    • v.11 no.2
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    • pp.61-66
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    • 2002
  • Double cropping system in a year in Kyoho grapes (Vitis labruscana L.) has currently been attempted in the plastic greenhouse. One of the problems in double cropping system is the promotion of bud break in summer season and shoot fertility. Effects of the control of soil moisture tension near the root zone and treatments of bud dormancy breaking agents on bud breaking in summer were examined to promote the bud break for the second fruiting. The lignification of shoots was induced in July or August by the control of soil moisture tension in root zone environment. The first shoot growth was almost the same as that in common plastic greenhouses. The highest bud break value appeared in the plot of cyanamide chemicals mixed with merit blue as over 75% bud break rate. The bud break rate in the discontinuing plot in irrigation showed significantly higher in bud break than that in the continuing plot in irrigation. Despite of the final high bud break rate, the time of bud break was irregular.

Screening of ${\beta}$-Catenin/TCF Transcription Factor Inhibitors in Medicinal Herb Extracts

  • Choe, Ye-Dang;Na, Byung-Jo;Park, Se-Yeon
    • The Journal of Korean Medicine
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    • v.32 no.3
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    • pp.35-43
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    • 2011
  • Objectives: This study was performed to screen target-specific inhibitors of ${\beta}$-catenin/TCF signaling whose functional activation plays an important role in early events in carcinogenesis. Methods: To investigate the activation or suppression of ${\beta}$-catenin/TCF transcription, we established a transiently transfected cell line with a constitutively active ${\beta}$-catenin mutant gene whose product is not degraded. This cell line was also co-transfected with luciferase reporter gene constructs containing either an optimized (TOPflash) or mutant (FOPflash) TCF-binding element. We investigated cytotoxic effects using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt (MTS) assay. To find effective inhibitors of ${\beta}$-catenin/TCF signaling from medicinal herbs, the crude extracts of 99 types of medicinal herbs were screened using a luciferase assay system in HEK-293 and SH-SY5y cells. Results: At a concentration of $50{\mu}g$/ml, extracts of Angelica koreanae radix, Cannabis sativa semen, Ephedrae intermedia Schrenk radix, and Vitis rotundifolia fruit showed the following inhibitory effects on ${\beta}$-catenin/TCF signaling: $40{\pm}5.6%$, $23{\pm}6.1%$, $8{\pm}5.1%$, and $22{\pm}9.8%$ in ${\beta}$-catenin-activated HEK-293 cells and $9{\pm}4.7%$, $39{\pm}8.1%$, $39{\pm}6.4%$, and $42{\pm}10.1%$ in ${\beta}$-catenin-activated SH-SY5y cells, respectively. Crude extracts of E. radix were isolated by silica gel column chromatography, and two non-polar fractions of these extracts showed inhibitory effects on ${\beta}$-catenin/TCF signaling. Conclusions: In this study, we established a transiently transfected cell line as a screening system and found that various medicinal herb extracts had inhibitory effects on ${\beta}$signaling.

Effect of Plant Growth Regulators on Multiple Shoot Formation and Elongation from Shoot Tip Cultures of Grape Species (포도의 경정배양에 의한 다아체형성 및 신장에 미치는 생장조절제의 영향)

  • 서정해;정재동;권오창
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.1
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    • pp.25-32
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    • 2001
  • Shoot tips of grape were cultured in uitro and tried to identify optimal culture conditions for regeneration, multiple shoot formation from meristemoid tissue and those subsequent elongation of multi-shoots. Healthy growing shoots were taken in early May, rinsed with running tap water, soaked in a neutral detergent and washed with soft brushing, and washed out with tap water, then sterilized with 10g Ca(ClO)$_2$/140 mL distilled water (Wilson's solution) for 5 min. Survival percentage of the cultures which were sterilized as above procedures was highly increased, compared with the other sterilized method. Propagation of multi-shoots from meristemoid showed a good response in 3/4 strength MS medium enriched with 0.1 mg/L NAA and 3.0 mg/L BA. Shoot elongation from multi-shooting clump well occurred in 3/4 strength MS medium supplemented with 80 mg/L adenine sulfate, 0.1 mg/L NAA and 1.0~2.0 mg/L BA.

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Selection of Antagonistic Bacteria for Biocontrol of Botrytis cinerea Causing Gray Mold on Vitis spp (포도 잿빛곰팡이병의 생물적 방제를 위한 길항세균 선발)

  • Seo, Sang-Tae;Park, Jong-Han;Han, Kyoung-Suk;Cheong, Seung-Ryong
    • Research in Plant Disease
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    • v.12 no.3
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    • pp.267-271
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    • 2006
  • Botrytis cinerea Pers. was found to be highly virulent to the grapevine plant, especially in greenhouse condition. Pseudomonas species play key roles for the biocontrol of many plant diseases especially in soil. Of the 83 isolates of Pseudomonas spp., a bacterial strain P84, isolated from tomato rhizosphere, was shown to suppress a wide range of phytopathogenic fungi in vitro. The isolate was identified as Pseudomonas putida on the basis of its bacteriological and genetic characteristics. The P. putida P84 strain carry the phlD gene for 2,4-diacetylphloroglucinol biosynthesis and may produce the antibiotics as an antagonistic mechanism involved in biocontrol. The antagonistic activity of the bacterium has a promising implication for its use as a biocontrol agent to control grapevine gray mold.

Application of Near Infrared Reflectance Spectroscopy as a Rapid Leaf Analysis Method to Evaluate Nutritional Diagnosis in Apple (Malus Domestica Borkh, Fuji) and grape(Vitis Labrusca, Campbell Early) (영양진단을 위한 신속한 엽분석 방법으로서 근적외분광분석기의 이용)

  • Seo, Young-Jin;Park, Man;Kim, Chang-Bae;Kim, Jong-Su;Yoon, Jae-Tak;Cho, Rae-Kwang
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.4
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    • pp.242-246
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    • 2000
  • The Near Infrared Reflectance Spectroscopy(NIR) was used to evaluate nutritional diagnosis for rapid leaf analysis method, 177 'Fuji' apple and 130 'Campbell Early' grape leaves were measured by Near Infrared reflectance spectra in the NIR region(1,100~2.500nm). Total nitrogen content was measured by kjelldhal distillation, after salycilic acid-sulfuric acid digestion. An empirical equation to predict total nitrogen content from its spectral signature was developed by adapting the Near Infrared Reflectance Spectroscopy analysis(NIRa) technique and the results were apple-0.965(R). 0.086(SEC), grape-0.926(R), 0.152(SEC). Standard Error of Prediction(SEP) of NIRa for predicting the total nitrogen of apple and grape leaves was 0.360 and 0.210, respectively. It was concluded that Near infrared reflectance spectroscopy analysis is promising method for rapid analysis of apple and grape leaves.

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Effect of Natural Plant Components on the Nitrite-scavenging (천연식물성분이 아질산염 소거에 미치는 영향)

  • 이수정;정미자;신정혜;성낙주
    • Journal of Food Hygiene and Safety
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    • v.15 no.2
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    • pp.88-94
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    • 2000
  • The purpose of this presents is to investigate, using natural food extracts(Green tea; Camellia sinesis, Du'chung; Eucommia ulmoides Oliver, Eu sung cho; Houttuynia cordate Thunb, Sam back cho; Saurus Chinensis, Baek hwa sa seal oho; Oldenladia diffusa Roxb., Laver; Porphyra tenera, Sea mustard; Undaria pinnatifda and Sea staghorn; Condium fragile) and juices(Sweet pepper; Capsicum annuum var. angulosum, Kale; Brassia oleracea var. acephala, Cucumber; Cucumis sativus, Onion; Allium cepa, Tomato; Lycopericon esculentum, Maesil; Prunus mume, Plum; Prunus salicina and Grape; Vitis spp.), the effect of natural plant components on the nitrite-scavenging under the different levels of pH. From the above mentioned extracts and juices, the content of vitamin C was detected, containing 65.1∼77.1 mg/100g, at the highest level in the Green tea, and followed by vegetables and fruits in order. The nitrite scavenging effect of teas, medicinal plants, and seaweed extracts, in the reaction system under the condition of pH 1.2, were 57.0∼100%, 50.0∼100%, and 18.0∼99.0%, respectively. Especially, the nitrite was scavenged to the level of 100%1 when 10 ml of kale and 5 ml of maesil Juice

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Ecological study of the flora at Tumen river area, border of North Korea and China (중국과 북한 접경지역 두만강 일대의 Flora에 대한 생태학적 연구)

  • Ahn, Young-Hee
    • Journal of Environmental Science International
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    • v.12 no.2
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    • pp.125-132
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    • 2003
  • This study was executed by China along with the Tumen river, which formed border of North Korea and China. As a result 91 family, 282 genera, 363 species, 57 variety 4 species, total 424 taxa of vascular plants were identified. The examination area was include the region that had various vegetation such as upper swampy land, seashore dune, a river, a high mountain grassy plain, deciduous tree zone, evergreen coniferous forest zone. The followings are representative plants, which found in an examination area but not in South Korea. Picea koraiensis, Larix gmelini var. principis-ruprechtii, Betula playphylla, Ulmus pumila, Populus koreana, Ledum palustre var. diversipilosum, Rhododendron parvifolium, Salix brachypoda, Phacelurus latifolius, Carex gotoi, Carex capricornis, Calla palustris, Aquilegia flabellata var. pumila, Lilium davuricum, Astragalus membranaceus, Trifolium lupinaster, Ledebourieila seselodeds, Polemonium racemosum, Scutellaria baicalensis, Linnaea borealis, Thladiantha dubia, Inula helenium, Trollius hondoensis, Hypocaeris ciliata, Aconitum kirinense, Aconitum arcuatum, Potentilla viscosa var. macrophylla. And there are 21 rare and endangered species, which Korean Forest Sevice designated, such as Aconitum koreanum, Acorus calamus var. angustatus, Polygonatum stenophyllum, Drosera rotundifolia, Iris setosa, Jeffersonia dubia, Lilium davuricum, Lilium cernum, Paeonia obovata, Penthorum chinense, Phacelurus latifolius, Rosa marretii, Sparganium stoloniferum, Thymus quinquecostatus, Trillium tschonoskii, Utricularia japonica, Tripterospermum japonicum, Vaccinium uliginosum, Vaccinium vitis-idaea, etc. Especially, plants like, Acanthopanax senticosus, Jeffersonia dubia and Lillum cernum which have high values of ornamental, and medical use are in an extinction crisis. High swampy land was formed to upper stream of Tumen river. It showed representative vegitation, like Carex sp., Scirpus radirans, Eriophorum latifolium. Wetlands plant and a dune plant were mixed in down stream area of Tumen river. On a dune, seashore plants, such as Carex kobomugi, Salsola collina, Raphanus sativus var. raphanistroides, Rosa rugosa, Scutellaria strigillosa, Linaria japonica, Phacelurus latifolius were appeared frequently.

Effect of Curcuminoids and Natural Plants Extract Mixture on the Cardiovascular System in Rats (흰쥐에서 Curcuminoid 및 이를 함유한 천연식물 혼합물이 심혈관계에 미치는 영향)

  • 안수현;이종호;박하림;권승택;고유석;손영덕;장양수;정광회
    • Journal of Nutrition and Health
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    • v.36 no.2
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    • pp.101-108
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    • 2003
  • Antiplatelet aggregation, anticoagulant and lipid-lowering drugs are clinically widely used for secondary preventive purpose in the cardiovascular patients, but there is no primary preventive agents to prevent these diseases. With the aim of developing effective primary agents for cardiovascular diseases, we tried to formulate an optimized mixture of natural plants extract containing Theae sinensis, Camelliae sinensis, Vitis vinifera, Gingko folium and curcuminoids from Curcuma longa and to evaluate its anti-thrombotic and anti-hypercholesterolemic effects in vivo. The inhibitory effect of curcuminoids on vascular smooth muscle cell proliferation and migration were also investigated in vitro. in the animal experiments treated with hyperlipidemic diet, oral treatment of curcuminoids and natural plants extracts mixture (100 mg/kg) into male Sprague Dawley rats for 7 week simultaneously inhibited platelet aggregation as well as improved lipid profile in the blood. Compared to control group, both of curcuminoids-treated and mixture-treated groups revealed significantly decrease of total cholesterol (24.4%, 28.6%), free cholesterol (25.1%, 24.0%), cholesterol ester (14.6%, 29.0%), LDL-cholesterol (27.0%, 32.0%) and triglyceride (15.0%, 31.0%), respectively. However, both groups showed increase of HDL-cholesterol (46.6% and 51.5%) . In particular, atherogenic index of curcuminoids and mixture treatment group was significantly decreased to 47.0% and 56.0%, respectively. Furthermore, oral treatment of curcuminoids and mixture significantly inhibited collagen-induced platelet aggregation (21.1% and 29.1%, respectively), compared to control group. The anti-thrombotic values of mixture was almost similar to that of aspirin treatment (100 mg/kg) group. These results suggest that the oral treatment of curcuminoids-based natural plant extract mixture improved cardiovascular conditions in hyperlipidemic rats.

Dual effects of a mixture of grape pomace (Campbell Early) and Omija fruit ethanol extracts on lipid metabolism and the antioxidant defense system in diet-induced obese mice

  • Han, Hye Jin;Jung, Un Ju;Kim, Hye-Jin;Moon, Byoung Seok;Cho, Su-Jung;Park, Yong Bok;Lee, Dong Gun;Choi, Myung-Sook
    • Nutrition Research and Practice
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    • v.9 no.3
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    • pp.227-234
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    • 2015
  • BACKGROUND/OBJECTIVES: We investigated the effects of a combination of grape pomace (Vitis labrusca, Campbell Early) and Omija fruit (Schizandra chinensis, Baillon) ethanol extracts on lipid metabolism and antioxidant defense system in diet-induced obese mice. MATERIALS/METHODS: Forty male C57BL/6J mice were divided into four groups and fed high-fat diet (control group, CON) or high-fat diet added 0.5% grape pomace extract (GPE), 0.05% Omija fruit extract (OFE) or 0.5% GPE plus 0.05% OFE (GPE+OFE) for 12 weeks. RESULTS: In contrast to the GPE- or OFE-supplemented groups, the GPE+OFE group showed significantly lower body weight and white adipose tissue weights than the CON group. Moreover, GPE+OFE supplementation significantly decreased plasma total cholesterol and increased the plasma HDL-cholesterol/total-cholesterol ratio (HTR) compared to the control diet. The hepatic triglyceride level was significantly lower in the GPE+OFE and GPE groups by increasing ${\beta}$-oxidation and decreasing lipogenic enzyme compared to the CON group. Furthermore, GPE+OFE supplementation significantly increased antioxidant enzyme activities with a simultaneous decrease in liver $H_2O_2$ content compared to the control diet. CONCLUSIONS: Together our results suggest that supplementation with the GPE+OFE mixture may be more effective in improving adiposity, lipid metabolism and oxidative stress in high-fat diet-fed mice than those with GPE and OFE alone.