• Title/Summary/Keyword: Vitis

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Pathogenic and Molecular Characteristics of Agrobacterium vitis strains isolated from Grapevine in Korea

  • Kim, J.G.;Kim, S.H.;Choi, J.E.;Lee, Y.K.;Kang, H.W.
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.120.2-120
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    • 2003
  • Agrobacterium vitis is a causal agent of crown-gall disease on grapevine. In Korea, grapevine variety (GeoBong) have severely been infected by the bacteria since stems of the variety were buried in soil for overwintering. Infection ratio over 70-80% was observed on 7 years old GeoBong grapevine in Ansung and Cheonan. PCR specific primers for A. vitis strains were designed using nucleotide sequences of vir A gene in Ti-Plasmid, pheA gene in chromosomal DNA and a URP-PCR polymorphic band. Three hundred bacterial strains were isolated from the different 80 galls formed on GeoBong grapevine in Cheonan and Ansung of Korea and were screened to identify A. vitis using the three specific PCR primers for Agrobacterium vitis. Twenty-four bacterial strains that are detected by the primers were further confirmed by pathogenicity and biochemical methods. To investigate the genomic diversity of the bacterial strains, twenty primers of 20 mer referred to universal rice primers (URP) were applied for PCR fingerprinting, Of them, URP2R and URP2F primers could effectively be used to detect polymorphism within the bacterial strains.

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Phytochemical Study on the Vitis thunbergii var. sinuata

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    • Korean Journal of Plant Resources
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    • v.9 no.1
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    • pp.55-62
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    • 1996
  • The caffeic acid, 4-O-$\beta$-D-glucopyranosyl caffeic acid, 4-O-$\beta$-D-glucopyranosyl-$\rho$-coumaric acid and 7-O-$\beta$-D-glucuronide of ($\pm$)-eriodictyol have been isolated from the root of Vitis thunbergii var. sinuata. The structures of compounds were determined by chemical and spectroscopic methods.

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DNA Topoisomerase I Inhibitory Activity of Stilbenes and Oligostilbenes from Leaf and Stem of Vitis amurensis

  • Kang, Na-Na;Ha, Do Thi;Park, Chang-Sik;Myung, Pyung-Keun;Bae, Ki-Hwan
    • Natural Product Sciences
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    • v.16 no.4
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    • pp.223-227
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    • 2010
  • The DNA Topoisomerase I (DNA Topo I) inhibitory effect of ten isolated compounds (1.10) from the leaf and stem of Vitis amurensis were examined. Among them, amurensin G (5) and r-2-viniferin (7) showed high potent inhibitory activity against DNA Topo I. DNA Topo I, an important target for anticancer drugs, can cause DNA breaks and play a key role during cell proliferation, transcription and repair. Thus, the results suggest that the selected compounds (5 and 7) from Vitis amurensis have a possibility as DNA Topo I-targeting anticancer agents.

Predation Efficiency of the Spider Tetragnatha squamata (Araneae: Tetragnathidae) to Tea Leafhopper Empoasca vitis(Insecta: Homoptera) (Tetragnatha squamata(거미 목: 갈거미 과)의 차말매미충, Empoasca vitis (곤충 강: 매미 목) 에대한 포식효과)

  • Heng-Mei Yan;Man-Yuan Liu;Joo-Pil Kim
    • Animal Systematics, Evolution and Diversity
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    • v.14 no.2
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    • pp.159-164
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    • 1998
  • The spider, Tetragnatha squamata and its prey Empoasca vitis, the tea leafhopper, were investigated in laboratory on the prey-predator relationship. The predator spider showed a significant response to the increase of the prey. The predation effiency and the response of predator to its density were examined as well. The result of the experiments are give as questions.

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Identification and Pathogenicity of Neophysopella vitis Causing Rust Disease on Meliosma myriantha in Korea

  • Dong Hwan Na;Jae Sung Lee;Young-Joon Choi;Ji-Hyun Park;Hyeon-Dong Shin
    • Research in Plant Disease
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    • v.29 no.3
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    • pp.299-303
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    • 2023
  • Rust symptoms on Meliosma myriantha trees have been noticed during disease surveys in Korea since 2010, with a high disease incidence frequently surpassing 90%. The causal fungus of the rust disease was identified as Neophysopella vitis based on the morphological investigation and molecular sequence analysis of the internal transcribed spacer (ITS) and large subunit (LSU) rDNA regions. This is the first report of rust disease caused by N. vitis on M. myriantha in Korea. A pathogenicity assay proved that M. myriantha serves as the aecial host of N. vitis as spermogonia and aeciospores were produced, which can infect the two uredinial hosts, Boston ivy (Parthenocissus tricuspidata) and Virginia creeper (Parthenocissus quinquefolia).

Antioxidant activity of solvent extracts from Vitis coignetiea skins (용매별 머루 과피 추출물의 항산화 효과)

  • Park, Hyun-Sil
    • Culinary science and hospitality research
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    • v.17 no.1
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    • pp.208-217
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    • 2011
  • This study explores the antioxidant activity of solvent extracts from Vitis coignetiea skins, and the result is as follows. Total phenolic compound and flavonoids contents were the highest in ethyl acetate extract, $53.4{\pm}1.2\;mg$/100 g and $644.7{\pm}3.7\;mg$/100 g, respectively. Electron donating abilities of solvent extracts from Vitis coignetiea skin were proportionally increased with concentration and ethyl acetate extract ($80.93{\pm}0.58%$) showed the higher activity than BHT($63.21{\pm}0.72%$) at concentration of $50\;{\mu}l$/mL. Reducing power of solvent extracts from Vitis coignetiea skin was the highest in ethyl acetate extract(1.769) at concentration of $1,000\;{\mu}l$/mL. TBARS of ethyl acetate extract was higher than ascorbic acid. Nitrite scavenging ability of solvent extracts from Vitis coignetiea skin(pH 1.2, $1,000\;{\mu}l$/mL) was the highest in ethyl acetate extract($92.5{\pm}2.4%$).

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PCR based Rapid Isolation of Agrobacterium vitis Strains in Korea and Their Pathogenical and Biochemical Characteristics (PCR 특이검출에 의한 국내 포도나무 혹병(Agrobacterium vitis) 균주의 신속 분리 및 병원학적, 생화학적 특성 비교)

  • Kim, Jong-Kun;Lim, Sun-Hwa;Lee, Dae-Sung;Choi, Jae-Eul;Yun, Hae-Keun;Park, Sang-Hun;Kang, Sung-Su;Kang, Hee-Wan
    • Research in Plant Disease
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    • v.12 no.3
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    • pp.205-212
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    • 2006
  • Agrobacterium vitis is a causal agent of crown-gall disease on grapevine. Bacterial strains were isolated from crown galls of different grapevine varieties in grapevine farms of Kyungbuk(Kimcheon), Chungbuk(Okcheon), Chungnam(Daejon, Choenan) and Kyeonggi(Suwon, Ansung) areas in Korea from 2002 to 2005. Primer sets, Phe A and VirA, which were derived from pectate lysase gene and virA gene of Ti-plasmid in A. vitis were used to detect A. vitis strains from crown galls. PheA and VirA primers amplified DNA fragments of 0.25 kb and 0.5 kb from fifty-one bacterialstrains. They formed crown galls on grapevine variety, Kyoho, or carrot disks with variable pathgenecity It was confirmed that the biochemical characteristics of 10 bacterial strains that was strong pathogene city on grapevine were mostly in agreement with type culture strains of A. vitis, showing growth in the presence of 2% NaCl, non-production of acid from melezitose and negative response in production of 3-Ketolactose.

Changes of Physicochemical Properties and Antioxidant Activities of Red Wines during Fermentation and Post-fermentation (적포도주들의 발효와 후발효 중 물리화학적 성질과 항산화활성의 변화)

  • No, Jae-Duck;Lee, Dae-Hyoung;Hwang, Young-Soo;Lee, Sang-Han;Lee, Jong-Soo
    • Microbiology and Biotechnology Letters
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    • v.36 no.1
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    • pp.67-71
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    • 2008
  • The goal of this study was to vinify four varieties of grapes, namely Vitis labrusca L (Gerbong), Vitis labrusca B (Campbell Early), Vitis labrusca (Muscat Bailey A) and Vitis hybrid (Sheridan), and to investigate the changes in the physicochemical properties and antioxidant activity of the red wines during fermentation and post-fermentation. The ethanol content of the four red wines varied only slightly from 11.4%-12.8%, indicating that no significant change occurred during the fermentation and post-fermentation. The total anthocyanin and phenol contents as bioactive compounds were the highest level in the Vitis labrusca B red wine. The antioxidant activity was also the highest of 88.9% after 10 days fermentation in the Vitis labrusca B red wine and showed from only 36.6% to 61.7% in the other red wines, though the range decreased to 33.1%-64.1% during post-fermentation for 120 days at $4^{\circ}C$. Our results show that the vitis labrusca B red wine has the potential to become a functional red wine because of its high antioxidant activity.

Biological control of grapevine crown gall (포도나무 줄기 혹병의 생물학적 방제)

  • Chung, Kwang-Jin;Shim, Jae-Seop;Chung, Bong-Koo
    • The Korean Journal of Pesticide Science
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    • v.2 no.2
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    • pp.97-101
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    • 1998
  • Agrobacterium vitis causes a crown gall disease in grapevine and that is one of the major hindrances for the wide cultivation and production of grapevine. We studied the possibility of biological control using selected biological control agent. One isolate from the infected soil, named as strain 27, was able to inhibit the biovar 1; A. tumefaciens C58 and Ach5, biovar 2; A. rhizogenes 13264, and biovar 3; A. vitis, in vitro and in vivo test. The putative biological control agent, A. radiobacter strain 27 was carrying the plasmid and the size of isolated plasmid was very similar to that of pAgK84 of A. radiobacter K84.

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Emergence of a New Rust Disease of Virginia Creeper (Parthenocissus quinquefolia) through a Host Range Expansion of Neophysopella vitis

  • Na, Dong-Hwan;Lee, Jae Sung;Shin, Hyeon-Dong;Ono, Yoshitaka;Choi, Young-Joon
    • Mycobiology
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    • v.50 no.3
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    • pp.166-171
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    • 2022
  • Virginia creeper (or five-leaved ivy; Parthenocissus quinquefolia) is one of the most popular and widely grown climbers worldwide. In September 2021, Virginia creeper leaves with typical rust symptom were found in an arboretum in Korea, with severe damage. Globally, there is no record of a rust disease on Virginia creeper. Using morphological investigation and molecular phylogenetic inferences, the rust agent was identified as Neophysopella vitis, which is a rust pathogen of other Parthenocissus spp. including Boston ivy (P. tricuspidata). Given that the two ivy plants, Virginia creeper and Boston ivy, have common habitats, especially on buildings and walls, throughout Korea, and that N. vitis is a ubiquitous rust species affecting Boston ivy in Korea, it is speculated that the host range of N. vitis may recently have expanded from Boston ivy to Virginia creeper. The present study reports a globally new rust disease on Virginia creeper, which could be a major threat to the ornamental creeper.