The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Biological control of grapevine crown gall

포도나무 줄기 혹병의 생물학적 방제

  • Chung, Kwang-Jin (Agricultural Technology Research Institute of Dongbuhannong Chemicals) ;
  • Shim, Jae-Seop (Department of Agricultural Biology, Chungbuk National University) ;
  • Chung, Bong-Koo (Department of Agricultural Biology, Chungbuk National University)
  • 정광진 (동부한농화학 농업기술연구소) ;
  • 심재섭 (충북대학교 농생물학과) ;
  • 정봉구 (충북대학교 농생물학과)
  • Published : 1998.09.30
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Abstract

Agrobacterium vitis causes a crown gall disease in grapevine and that is one of the major hindrances for the wide cultivation and production of grapevine. We studied the possibility of biological control using selected biological control agent. One isolate from the infected soil, named as strain 27, was able to inhibit the biovar 1; A. tumefaciens C58 and Ach5, biovar 2; A. rhizogenes 13264, and biovar 3; A. vitis, in vitro and in vivo test. The putative biological control agent, A. radiobacter strain 27 was carrying the plasmid and the size of isolated plasmid was very similar to that of pAgK84 of A. radiobacter K84.

최근에 재배면적이 급격히 증가하고 있는 포도에 난방제 병해인 줄기혹병의 길항세균을 선발하여 생물학적 방제의 가능성을 조사한 결과 포도 줄기혹병 이병포장으로부터 채취한 토양에서 길항균 strain 27를 선발하여 in vitro 및 in vivo bioassay결과 biovar 1 병원균인 A. tumefaciens C58과 Ach5, biovar 2 병원균인 A. rhizogenes 13264 및 biovar 3 병원균인 A. vitis 등을 모두 억제시키는 효과를 보였으며, Strain 27의 세균학적 특성은 pAgK84와 비슷한 크기(46 Kb)의 plasmid를 갖고 있음을 확인하였다.

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