• Title/Summary/Keyword: Visualize-5

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Microvascular Contrast Image in Portal Veins of Rat using Micro-CT (마이크로 CT를 이용한 BALB/C(흰쥐) 간문맥의 미세혈관 조영 영상)

  • Lee, Sang-Ho;Lim, Cheong-Hwan;Jung, Hong-Rayng;Han, Beom-Hee;Mo, Eun-Hee;Chai, Kyu-Yun
    • The Journal of the Korea Contents Association
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    • v.10 no.9
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    • pp.259-266
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    • 2010
  • The study focuses on the value of Micro CT, a high resolution X-ray imaging device, by using it on rats to observe the overall portal vein image of the liver and the microvasculature of each lobes, visualize the 4 segmental lobes and acquire 3D image of the microvasculature through the reconstruction of sectional image data. Less of the damage to liver of the 5 mice, the device was able to separate the liver into 4 segmental lobes and displayed the 4 portal vein microvasculature in 2D. By using the 3D MIP technique, observation of the whole portal vein system microvasculature in 3D image was made possible along with each of the portal vein segment's branches until the 6th branch. Measured the size of 6branch, the average was measured at 1branch : $0.51mm{\pm}0.08$, 2 branch : $0.32mm{\pm}0.12$, 3 branch : $0.23mm{\pm}0.11$, 4 branch : $0.19mm{\pm}0.08$, 5 branch : $0.13mm{\pm}0.06$, 6 branch : $70.5{\mu}m{\pm}14.1$. The 3D image and the images of the microvasculatures in the result of study proved that the Micro-CT can be considered many useful device in obtaining high resolution images.

Emergency Room access by Population Density and distance of Daegu Metropolitan city (대구광역시의 인구밀집과 거리에 따른 응급실 접근성)

  • Kim, Myung-Gwan;Han, Seung-Woo;Kim, Ki-Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.7
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    • pp.218-223
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    • 2020
  • This study examined the accessibility of emergency rooms according to the population density and distance in Daegu Metropolitan City to help improve the quality and emergency medical accessibility problems in Daegu Metropolitan City. To observe the geographical requirements, the number of population in eup, myeon, dong, available emergency, and available emergency compared to the population were mapped through the S-GIS of Statistics Korea to visualize the data based on the 3km radius. To determine the difference in accessibility to emergency rooms for each district in Daegu Metropolitan City, the data were analyzed by ANOVA and Scheffe. According to the average number of emergencies available in Daegu Metropolitan City within a 3-kilometer radius were 5.7 in Jung-gu, 5.0 in Nam-gu, 1.6 in Buk-gu, 0.4 in Dong-gu, 2.4 in Seo-gu, 0.9 in Suseong-gu, 3.5 in Dalseo-gu, and 0.1 in Dalseong-gun; there was a statistically significant difference (p<.001). The available emergency within a 3km radius and available emergency per 1,000 people were concentrated in the center. Therefore, it may be necessary to institute an urban emergency medical network by establishing a point that serves as an intermediate hub to provide emergency medical care to citizens far from the center.

PET Imaging of Click-engineered PSMA-targeting Immune Cells in Normal Mice

  • Hye Won Kim;Won Chang Lee;In Ho Song;Hyun Soo Park;Sang Eun Kim
    • Journal of Radiopharmaceuticals and Molecular Probes
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    • v.8 no.2
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    • pp.53-61
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    • 2022
  • This study aimed to increase the targeting ability against PSMA in cell therapy using metabolic glycoengineering and biorthogonal chemistry and to visualize cell trafficking using PET imaging. Cellular membranes of THP-1 cells were decorated with azide(-N3) using Ac4ManNAz by metabolic glycoengineering. Engineered THP-1 cells were conjugated with DBCO-bearing fluorophore (ADIBO-Cy5.5) for 1 h at different concentrations and analyzed by confocal fluorescence microscopy and flow cytometry. For PSAM ligand conjugation to THP-1 cells, Ac4ManNAz treated THP-1 cells were incubated with DBCO-PSMA ligand (ADIBO-GUL) at a final concentration with 100 µM for 1 h. To evaluate the effect on cell recognition, PSMA ligand conjugated THP-1 cells(as effectors) were co-cultured with PSMA positive 22RV1 (as target cells) at 3 : 1 a effector-to-target cell (E/T) ratio. The interaction between THP-1 and 22RV1 was monitored by confocal fluorescence microscopy. For preparing the radiolabeled THP-1, the cells were treated at the activity of ~ 740 kBq of [89Zr]Zr(oxinate)4/5 × 106 cells. Radiolabeled cells were analyzed for determination of cell-associated radioactivity by gamma counting and viability using MTS assay. In the cytotoxicity assay, THP-1 cells did not have any cytotoxicity even when the Ac4ManNAz concentration was 100 µM. In confocal microscopy and flow cytometry, THP-1 cells were efficiently labeled ADIBO-Cy5.5 in a dose-dependent manner, and the dose of 100 µM was the optimal concentration for the following experiments. The clusters of PSMA ligand-conjugated THP-1 cells and 22RV1 cells were identified, indicating cell-cell recognition over the cell surface between two types of cells. Cell radiolabeling efficiency was 54.5 ± 17.8%. THP-1 labeled with 0.09 ± 0.03 Bq/cell showed no significant cytotoxicity compared to unlabeled THP-1 up to 7 days. We successfully demonstrated that Ac4ManNAz treated cells were efficiently conjugated with ADIBO-GUL for preparing the PSMA-targeting cells, and [89Zr]Zr(oxinate)4 could be used to label cells without toxicity. It suggested that PSMA-ligand conjugated cell therapy could be improved cell targeting and be monitored by PET imaging.

Development of 3D Mapping System for Web Visualization of Geo-spatial Information Collected from Disaster Field Investigation (재난현장조사 공간정보 웹 가시화를 위한 3차원 맵핑시스템 개발)

  • Kim, Seongsam;Nho, Hyunju;Shin, Dongyoon;Lee, Junwoo;Kim, Hyunju
    • Korean Journal of Remote Sensing
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    • v.36 no.5_4
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    • pp.1195-1207
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    • 2020
  • With the development of GeoWeb technology, 2D/3D spatial information services through the web are also has been used increasingly in the application of disaster management. This paper is suggested to construct a web-based 3D geo-spatial information mapping platform to visualize various spatial information collected at the disaster site in a web environment. This paper is presented a web-based geo-spatial information mapping service plan for the various types of 2D/3D spatial data and large-volume LiDAR point cloud data collected at the disaster accident site using HTML5/WebGL, web development standard technology and open source. Firstly, the collected disaster site survey 2D data is constructed as a spatial DB using GeoServer's WMS service and PostGIS provided an open source and rendered in a web environment. Secondly, in order to efficiently render large-capacity 3D point cloud data in a web environment, a Potree algorithm is applied to simplifies point cloud data into 2D tiles using a multi-resolution octree structure. Lastly, OpenLayers3 based 3D web mapping pilot system is developed for web visualization of 2D/3D spatial information by implementing basic and application functions for controlling and measuring 3D maps with Graphic User Interface (GUI). For the further research, it is expected that various 2D survey data and various spatial image information of a disaster site can be used for scientific investigation and analysis of disaster accidents by overlaying and visualizing them on a built web-based 3D geo-spatial information system.

Application of 3D Picture Control to the Simulator to Visualize the Field Demonstration Data (실증 Data 시각화를 위한 풍력시스템 Simulator 3D 시각화 작업 구현)

  • Choi, Hyojin;Kim, Young Chan;Chun, Chinwha
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.11a
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    • pp.187-187
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    • 2010
  • 풍력을 이용한 풍력발전기의 전력변환 시스템을 연구개발하기 위해서는 바람의 특성을 실내에서 구현할 수 있는 모의시험장치인 시뮬레이터가 요구된다. 모의시험장치는 바람을 받아 회전하는 블레이드 대신 풍속 값을 입력하여 전동기가 발전기에 토오크(torque)를 공급하게 된다. POSTECH 풍력에너지연구소는 2003년 750kW급 KBP-750D 풍력발전기용 모의시험장치에 이어, 2007년 2MW Gearless형 풍력발전기(KBP-2000M)의 400:1 축소모델인 5.83kW 모의시험 장치를 제작 완료하였다. (주)플라스포사는 모의시험장치를 이용하여 고효율의 전력변환장치 설계와 인버터 시스템 제어 알고리즘을 개발하였고, (주)유니슨은 2MW 풍력발전기용 통합연동제어 시스템을 제작 완료하여 2009년부터 대관령 실증단지에 초도품을 설치하고 실증운영 중에 있다. 본 논문은 대관령 실증단지에서 실시한 2MW 풍력시스템 실증과정에서 획득한 풍향과 풍속 데이터를 시각적으로 쉽게 보고 이해할 수 있도록 3D모델 처리방안을 적용하여 모의시험 장치에 구현한 내용이다. 풍력발전기 개발은 현재 대형화 추세에 있으며 연구개발 단계에서 풍력발전기의 발전 및 제어부분의 기능적 안정성 확보와 효율성 증대를 위한 적절한 시험은 필수적이다. 그러나 풍력발전기의 특성상 타워를 건설하고 회전자 블레이드를 사용하여 시험한다는 것은 설치 공간과 비용의 제약이 따른다. KBP-2000M (2MW) 모의시험장치는 풍력발전기와 같은 대형장비를 연구 개발하는데 필수적이며, 실증을 통해 획득한 Data를 적용함으로서 재품의 문제점을 찾아내고 성능향상을 도모하는데 필요하다. 실증 Data를 시각적으로 3D로 표현함으로서 현장의 풍속/풍향 변화를 사실감 있게 느끼게 되었다. 앞으로도 풍력발전기를 연구개발하거나 성능향상을 위해서는 적절한 제어와 안전을 위한 연동장치를 갖춘 스케일 다운된 모의시험장치 구축이 요구될 것이다. 풍력발전기의 실증과정에서 획득한 데이터를 모의시험장치에 적용(Feedback)하여 지속적인 성능향상을 높여나갈 것이다.

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Integration of Wireless Body Area Networks (WBANs) and WAN, WiMAX and LTE

  • Hu, Long;Dung, Ong Mau;Liu, Qiang;Han, Tao;Sun, Yantao
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.7 no.5
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    • pp.980-997
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    • 2013
  • Nowadays, wireless communication has a great advantage in technology. We use wireless devices almost in all expected life such as: entertainment, working and recently in the healthcare area, where Wireless Body Area Networks (WBANs) become a hot topic for researchers and system designers. Recent work on WBANs focus on related issues to communication protocol, especially ZigBee network is fine tuned to meet particular requirements in healthcare area. For example, some papers present real-time patient monitoring via ZigBee communication given the short distance between body sensors and remote devices, while the other work solve the limited coverage problem of Zigbee by designing mechanisms to relay Zigbee data to other types of wire or wireless infrastructure. However, very few of them investigate the scenarios of ZigBee coexisting or integrated with other networks. In this paper, we present the real-time data transmission from ZigBee end devices to Wide Area Network (WAN), Worldwide interoperation for microwave access network (WiMAX) and Long Term Evolution network (LTE). We provide in detail the ZigBee gateway components. Our simulation is conducted by OPNET, we visualize many topology network scenarios in ZigBee hybrid system. The results in simulation show that ZigBee end devices can successfully transmit data in real-time to other network end devices.

A Study on Hampyeong Butterfly Festival Cultural Products Design Contents using CAD - Focus on Adobe illustrator CS2 - (CAD를 활용한 함평나비축제 문화상품 디자인 콘텐츠 연구 - Adobe Illustrator CS2를 중심으로 -)

  • Lee, Sang-Phil;Kim, Seon-Hong
    • The Research Journal of the Costume Culture
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    • v.15 no.5
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    • pp.760-769
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    • 2007
  • Cultural commodity is a tourism souvenir for tourists with a high added value in terms of economy. When it excellently represents the region and is based upon its tradition, the positive images of the place can be originated. In this way, the formation of benevolent images has economically considerable effects that can promote the competitive power of the area. The purpose of this study is to develop the pattern design of cultural commodity related to Hampyeong Butterfly Festival. The software to be used is Adobe illustrator CS2, which is a Vector Graphic software, and by utilizing the program, the pattern for the cultural goods will be produced. The forms of the pattern are All over Pattern, which is one of the arrangement methods used the most in a necktie field, and Stripe Pattern. After designing with those two patterns, the design is applied on neckties. Like this, by designing through the Vector Graphic software which we can easily get an access to, the concept of design is visualized; therefore, we can prevent the commodity development that can be conducted out of the concept. By making it possible to visualize the examination by various forms or color mixture, the time is shortened, and throughout reproduction of the object, adjustment of the size, colorway, and reusing or remanufacturing the image, we can check the result of design before hand to reduce the time and expenses required.

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Characterization and Electron Microscopic Visualization of the Plasmid and the Gene Expression in the Escherichia coli (E. coli의 유전자 발현 및 플라스미드 DNA 구조의 특성에 관한 연구)

  • Kim, Sung-Jun;Park, Yeal;Lee, Sook-Young;Kim, Hong-Seob;Kim, Woo-Kap
    • Applied Microscopy
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    • v.20 no.2
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    • pp.57-70
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    • 1990
  • Characterization and electron microscopic visualization of the plasmid and the gene expression of Escherichia coli were carried out. Transcriptional units of active structural genes were observed after lysis of Escherichia coli cells. The ribosomes attached to the E. coli genome on mRNA molecule as polyribosomes. From this gradient of polyribosome length, we estimated location of mRNA synthesis initiation site. In this experiment, a granule is ofen present which may correspond to a RNA polymerase at the promoter site. pOX1, pOX7, pOX7A, $pOX7{\Delta}1$, pSTP36, pSTP21, pBR322, and pJH12 were visualized by way of electron microscope, and their estimated sizes were determined to be $5.70{\pm}0.08{\mu}m,\;2.15{\pm}0.10{\mu}m,\;2.14{\pm}0.12{\mu}m,\;7.39{\pm}0.08{\mu}m,\;4.03{\pm}0.04{\mu}m,\;1.50{\pm}0.03{\mu}m\;and\;1.25{\pm}0.09{\mu}m$ respectively. One micrometer of measured length corresponded to about 3.0 Kb. Mica-press adsorption method that allows selectivs visualization of the plasmid DNA released in situ from the bacterial cell is rapid and useful for visualization of plasmids. The released plasmid DNA was adsorbed preferently on mica in a divalent cation-free solution. Miller chromatin-spreading method was useful to observe the plasmid and transcripts. BAC method and cytochrome C monolayer were useful to observe the plasmid DNA. Our ability to visualize ultrastructural aspects of the expression of E. coli has given us a unique tool with which to study the regulation the level of an individual gene.

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The clinical study of Digital Infrared Thermographic Imaging on Hwa-byung(火病) patients (전신체열촬영(全身體熱撮影)에 의한 화병환자(火病患者)의 임상적(臨床的) 연구(硏究))

  • Kim Tae-Heon;Lyu Yeoung-Su
    • Journal of Oriental Neuropsychiatry
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    • v.10 no.1
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    • pp.133-146
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    • 1999
  • First I chose a patient group and a comparative group composed of 23 members each. Then the body temperature differences in these groups was taken with D.I.T.I. The results are as follows. 1. Average body temperature of the Hwa-Byung patient group is $36.6^{\circ}C$ and that of the comparative group is $36.4^{\circ}C$. So there is no meaningful body temperature difference. 2. The hwa-Byung patient group has higher temperature than the comparative group by ${\triangle}T>1.0^{\circ}C$ at the following acupunture points in these body parts upper and lower, left and right, anterior and posterior, trunk and limb. Especially, the body temperature difference was clear in the CV-17 (jun-Joong) and the GV-4(Myung-moon). 3. When the body temperature was compared in the upper and lower part of the back, it had meaningful differences at the upper and lower acupunture points of the back in the Hwa-Byung patient group but not in the comparative group. 4. When the body temperature was compared in the left and right part of the body, its average difference was ${\triangle}T>1.0^{\circ}C$ in the two groups and the result were meaningless. 5. When body temperature was compared in the anterior and posterior part of the body, its average difference was ${\triangle}T>1.0^{\circ}C$ in the two groups and the result were meaningless. 6. When body temperature was compared in the trunk and limb part of the body, its difference in the patient group was meaningful compared with the non-patient group. From the above results, I think that D.I.T.I. could be used to objectively visualize heat sensation of the Hwa-Byung patients and make an objective concept of Han Yul in oriental medicine.

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Skin Thickness of the Anterior, Anteromedial, and Anterolateral Thigh: A Cadaveric Study for Split-Skin Graft Donor Sites

  • Chan, Jeffrey C.Y.;Ward, John;Quondamatteo, Fabio;Dockery, Peter;Kelly, John L.
    • Archives of Plastic Surgery
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    • v.41 no.6
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    • pp.673-678
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    • 2014
  • Background The depth of graft harvest and the residual dermis available for reepithelization primarily influence the healing of split-skin graft donor sites. When the thigh region is chosen, the authors hypothesize based on thickness measurements that the anterolateral region is the optimal donor site. Methods Full-thickness skin specimens were sampled from the anteromedial, anterior, and anterolateral regions of human cadavers. Skin specimens were cut perpendicularly with a custom-made precision apparatus to avoid the overestimation of thickness measurements. The combined epidermal and dermal thicknesses (overall skin thickness) were measured using a digital calliper. The specimens were histologically stained to visualize their basement membrane, and microscopy images were captured. Since the epidermal thickness varies across the specimen, a stereological method was used to eliminate observer bias. Results Epidermal thickness represented 2.5% to 9.9% of the overall skin thickness. There was a significant difference in epidermal thickness from one region to another (P<0.05). The anterolateral thigh region had the most consistent and highest mean epidermal thickness ($60{\pm}3.2{\mu}m$). We observed that overall skin thickness increased laterally from the anteromedial region to the anterior and anterolateral regions of the thigh. The overall skin thickness measured $1,032{\pm}435{\mu}m$ in the anteromedial region compared to $1,220{\pm}257{\mu}m$ in the anterolateral region. Conclusions Based on skin thickness measurements, the anterolateral thigh had the thickest epidermal and dermal layers. We suggest that the anterolateral thigh region is the optimal donor site for split-skin graft harvests from the thigh.