• Environmental Mutagens and Carcinogens
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• v.17 no.2
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• pp.71-77
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• 1997

The single cell gel electrophoressis(SCGE) assay, also known as the comet assay, is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakage in mammalian cells. The SCGE or comet assay is a promising test for the detection of DNA damage and repair in individnal cells. It has widespread potential applications in DNA damage and repair studies, genotoxicity testing and biomonitoring. In this microgel electrophoresis technique, cells are embedded in agarose gel on microscope slides, iysed and electrophoresed under alkaline conditions. Cells with increased DNA damage display increased migration of DNA from the nucleus towards the anode. The length of DNA migration indicates the amount of DNA breakage in the cell. The comet assay is also capable of identifying apoptotic cells which contain highly fragmented DNA. Here we review the development of the SCGE assay, existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA and the potential applications of the technique.

• Journal of Aerospace System Engineering
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• v.6 no.3
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• pp.13-18
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• 2012

Every 3000 hour an aircraft is stricken by a lightning. Also the lightning damage to the aircraft during flight are continually occurred due to extreme weather phenomena such as global warming. Under the airworthiness standards, the aircraft must be designed to protect lightning. To show compliance for lightning, the test should be conducted by the actual lightning current and voltage waveform for the actual aircraft or parts. After test, structure damage is detected via visual inspection or NDI. Structure substantiation for damage is to show retaining limit or near limit load capability. This is conducted by test or analysis based on test. Thus, the aircraft should retain structural strength to land safely, even though the damage of aircraft fuselage from Lightning strike are occurred.

• Journal of Korean Society of Forest Science
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• v.89 no.2
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• pp.145-160
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• 2000

This study is focused to the national park of Korean typical Sea Hally$\check{o}$ Haesang, and its visual resources and practiced inspect course by the way of suppositions and tests, to show the visual resource management objectively, and that of qualitative basic data. Accordingly by measuring the physical amount spatial structure with the visual amount originated from the Mesh Analyzing Method and the Visual Preference from the Scenic Beauty Estimation(S.B.E.) method and analyzed the valuation of the visual resource by Iverson method. Spatial image structure measured by Semantic Differential(S.D.) Scale was shown through the factor analysis algorithm for the analyzing psychological amount and examined the flowing out of decisive factor and the objective importance related to the mutual factors by appling the measurement of the visual quality. As a national Park, the visual factors that have natural landscape harmonized with forest, sky, surface of the water, curious stones and rocks, and temples should be escalated their values affirmatively so as to be the scenery of pointed direction and enjoyable, and it is of more needed for visual resource and its' controlling technique to make artificial structures more intentional planning and systemical setting. When we are viewing the improvement for the national park along with the visual resource management, reasonable level of development is needed, because when men interference surpass plantations and leasts will be damaged and the quality of natural landscape can be lowered, so it is needed to set up a management end, tangibly or clearly; and it is permitted limit coming and going ablably by accounting the suitable number for availing. But the controling end should be set in every level, positive management, very actively within the permissive varcability. It is the main business for the national park to prevent the damage from human for their gay life or to prevent the damage of a land carpet, and to restorate for the visual resource management.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2862-2867
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• 2008

High-temperature components of gas turbine operated for certain period of time can be reused by being repaired or rejuvenated. In case of the gas turbine combustion liners, the biggest and the most important one in the high-temperature components, come in a repair shop after operated for 8,000 or 12,000 hours according to the model and go through the repair and rejuvenation in order to be reused. A stated combustion liner is the first channel which has the combustion gas reached a nozzle from a fuel nozzle. Materials and coating properties of old and new model combustion liners were investigated. To repair these components after the visual inspection, the coatings of combustion liners were removed and then FPI(Fluorescent Penetrant Inspection), a kind of the NDI(Non-Destructive Inspection), was conducted. Damage patterns and the number of the damaged components were classified and analyzed based on data provided from the visual inspection over a long period of time. Focusing on the difference between old model and new model combustion liners, we analyzed the damage distribution and changes and consequently concluded that new model combustion liner would increase repair rate.

• Journal of the Korean Geotechnical Society
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• v.20 no.1
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• pp.49-59
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• 2004

A GIS-based tunnelling risk management system(GIS-TURIMS) was developed as a product of this study, The developed system uses ArcView 8.1 as a basic platform and the built-in interface(VBA) has been used to perform first-order simplified analyses for the prediction of tunnelling-induced ground movements and building damage assessment. The main emphasis of this study was to develop a working framework that can be used in the perspective of tunnelling risk management. The developed system is capable of carrying out cornputationally intensive analyses for ground movement prediction as well as buildings/utilities damage assessment taking full advantage of the GIS technologies. This paper describes the concept and details of the GIS-TURIMS development and implementation.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2001.05a
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• pp.577-582
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• 2001

Identification of damage of structures has recently received considerable attention in the light of maintenance and safety assessment under service loads. In buildings, the current techniques of safety assessment largely depend on partial experiments such as visual inspection, destructive and nondestructive tests which lead to overconsumption of time and cost as well as higher labor intensity. Therefore, a new trial for safety assessment is urgently needed today. In this respect, the vibration characteristics of buildings have been applied steadily to obtain a damage index of the whole building, but it cannot be established as a practical method until now. This study is aimed at investigating the application of damage identification methods using vibration characteristics of building. Numerical tests are performed on a apartment building. From the test results, it is observed that severity and location of damage can be estimated with a relatively small error by using natural frequency and mode shape data.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2017.11a
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• pp.65-66
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• 2017

Early-frost damages easily take place in smaller and thinner walls and slabs. In case of slabs, it is difficult to visually determine the depth of early-frost damage. As such, the current study aims to determine the depth of early-frost damage caused to concrete structures due to bad curing in the winter. As a result, the study found that the depth of early-frost damages increased from the top as the atmospheric temperature on the concrete surface decreased. The changes in the color allowed the observer to easily identify the depth of early-frost damage with the naked eye. In particular, the color difference between potentially damaged parts and undamaged parts were the greatest around thirty minutes of drying after wetting.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.1206-1208
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• 2003

The extraction of earthquake damage from remote sensed imagery requires high spatial resolution and temporal effectiveness of acquisition of imagery. The analog photographs and visual interpretation were taken traditionally. Now it is possible to acquire damage information from many commercial high resolution RS satellites. The key techniques are processing velocity and precision. The authors developed the automatic / semiautomatic image process techniques including feature enhancement, and classification, designed the emergency Earthquake Damage and Losses Evaluate System based on Remote Sensing (RSEDLES). The paper introduced the functions of RSEDLES as well as its application to the earthquakes occurred recently.

• Journal of Biosystems Engineering
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• v.40 no.4
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• pp.409-416
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• 2015

Purpose: This study evaluates a new banana bulk packaging method under the real transport conditions of Sri Lanka. Methods: A field evaluation of optimized 8-mm thick Styrofoam sheets used as the cushioning material was applied. A trial transport was conducted from Thambuttegama to Colombo using a medium-sized open truck, with banana leaves as the control material. Data were recorded at the farmer, transporter, retailer, and consumer stages of the supply chain. Mechanical damage, physiological loss in weight, fruit firmness, total soluble solids, ripeness index, visual quality ratings, and the physical damage index of the bananas were measured at each stage. A cost-benefit analysis was also conducted for both packaging methods. Results: The 8-mm styrofoam sheets significantly reduced (p < 0.05) the mechanical damage from 26.3% to 12.9% compared to the conventional method for long-distance transport, and the physiological loss in weight showed a decrease of 2.88%. The loss of firmness of the fruits followed a simmilar pattern for both methods until reaching the retailer, but at the consumer was significantly higher (p < 0.05) for the control. However, the physical damage index at the retail stage for the control showed symptoms of physical injury, whereas the bananas transported using the cushioning materials exhibited only minor symptoms. Further, the visual quality of the fruits after transport from the farmer to the consumer was preserved, which is one of the main factors affecting consumer preference and retail price. The proposed method increases the profit margin by 51.2% for Embul bananas owing to the reduced postharvest losses. Conclusion: The 8-mm thick Styrofoam sheets reduced the physical damage to the bananas, with the quality parameters maintained at the prefered level. Moreover, profits may be increased.

• Structural Engineering and Mechanics
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• v.25 no.1
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• pp.39-52
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• 2007

This paper describes an experimental study on structural health monitoring of a 1:3-scaled one-story concrete frame subjected to seismic damage and retrofit. The structure is tested on a shaking table by exerting successively enhanced earthquake excitations until severe damage, and then retrofitted using fiber-reinforced polymers (FRP). The modal properties of the tested structure at trifling, moderate, severe damage and strengthening stages are measured by subjecting it to a small-amplitude white-noise excitation after each earthquake attack. Making use of the measured global modal frequencies and a validated finite element model of the tested structure, a neural network method is developed to quantitatively identify the stiffness reduction due to damage and the stiffness enhancement due to strengthening. The identification results are compared with 'true' damage severities that are defined and determined based on visual inspection and local impact testing. It is shown that by the use of FRP retrofit, the stiffness of the severely damaged structure can be recovered to the level as in the trifling damage stage.