• Journal of Applied Biological Chemistry
• /
• v.57 no.2
• /
• pp.165-170
• /
• 2014

The stems of Viburnum erosum were extracted with 80% MeOH. The concentrated extract was partitioned with EtOAc, n-BuOH, and $H_2O$. From the EtOAc and n-BuOH fractions, four compounds were isolated through the repeated $SiO_2$, octadecyl silica gel, and Sephadex LH-20 column chromatographies. Based on NMR, MS, and IR spectroscopic data, the chemical structures were determined as betulinic aldehyde (1), koaburside (2), (6R,7E,9R)-9-hydroxymegastigma-4,7-dien-3-one-9-O-${\beta}$-D-glucopyranoside (3), and byzantionoside B (4). All the compounds were isolated for the first time from the stems of Viburnum erosum.

• Korean Journal of Plant Taxonomy
• /
• v.50 no.1
• /
• pp.8-16
• /
• 2020

Complete chloroplast genome sequences provide detailed information about any structural changes of the genome, instances of phylogenetic reconstruction, and molecular markers for fine-scale analyses. Recent developments of next-generation sequencing (NGS) tools have led to the rapid accumulation of genomic data, especially data pertaining to chloroplasts. Short reads deposited in public databases such as the Sequence Read Archive of the NCBI are open resources, and the corresponding chloroplast genomes are yet to be completed. The V. dilatatum complex in Korea consists of four morphologically similar species: V. dilatatum, V. erosum, V. japonicum, and V. wrightii. Previous molecular phylogenetic analyses based on several DNA regions did not resolve the relationship at the species level. In order to examine the level of variation of the chloroplast genome in the V. dilatatum complex, raw reads of V. dilatatum deposited in the NCBI database were used to reconstruct the whole chloroplast genome, with these results compared to the genomes of V. erosum, V. japonicum, and three other species in Viburnum. These comparative genomics results found no significant structural changes in Viburnum. The degree of interspecific variation among the species in the V. dilatatum complex is very low, suggesting that the species of the complex may have been differentiated recently. The species of the V. dilatatum complex share large unique deletions, providing evidence of close relationships among the species. A phylogenetic analysis of the entire genome of the Viburnum showed that V. dilatatum is a sister to one of two accessions of V. erosum, making V. erosum paraphyletic. Given that the overall degree of variation among the species in the V. dilatatum complex is low, the chloroplast genome may not provide a phylogenetic signal pertaining to relationships among the species.

• Korean Journal of Plant Taxonomy
• /
• v.48 no.3
• /
• pp.206-217
• /
• 2018

The nucleotide sequences of the chloroplast rbcL, matK, and psbA-trnH and nuclear internal transcribed spacer (ITS) regions were determined from all species of Viburnum in Korea with multiple accessions to reconstruct the phylogeny and to evaluate the utility of the DNA sequences as DNA barcodes. The results of phylogenetic analyses of the cpDNA and ITS data are consistent with the findings of previous studies of Viburnum. Four morphologically closely related species, V. dilatatum, V. erosum, V. japonicum, and V. wrightii, were included in a strongly supported sister clade of V. koreanum and V. opulus. Viburnum odoratissimum is suggested to be sister to the V. dilatatum/V. koreanum clade in the cpDNA data, while V. odoratissimum is a sister to V. furcatum in the ITS data. Viburnum burejaeticum and V. carlesii are strongly supported as monophyletic. Our analyses of DNA barcode regions from multiple accessions of the species of Viburnum in Korea confirm that six out of ten species in Korea can be discriminated at the species level. The V. dilatatum complex can be separated from the remaining species according to molecular data, but the resolution power to differentiate a species within the complex is weak. This study suggests that regional DNA barcodes are useful for molecular species identification in the case of Viburnum when flowering or fruiting materials are not available.

• Journal of agriculture & life science
• /
• v.51 no.1
• /
• pp.57-67
• /
• 2017

We conducted this study to investigate vegetation structure and soil conditions of six Viburnum erosum Thunb.(V. erosum) populations which showed high dominance. A total of 68 woody plants were observed to distribute: 13 in tree layer; 38 in subtree layer; and 48 in shrub layer. Based on the Mean Importance Value(MIV), the dominant tree species in the V. erosum populations were as follows in order of: Acer pseudosieboldianum(Pax) Kom., Callicarpa japonica Thunb., Indigofera kirilowii Maxim. ex Palib., Lindera obtusiloba Blume, Prunus sargentii Rehder, Quercus variabilis Blume, Rhododendron mucronulatum Turcz. and Vaccinium oldhamii Miq. In paticular, the species observed in all of the study sites were Quercus serrata Murray and Styrax japonicus Siebold & Zucc. It was found that the presence of V. erosum was positively correlated with that of Albizia julibrissin Durazz. and Symplocos chinensis f. pilosa (Nakai) Ohwi, while negatively correlated with that of Platycarya strobilacea Siebold & Zucc. According to the result, means of species diversity(H'), maxim species diversity(H' max), evenness(J') and dominance(1-J') were measured at 0.887, 1.102, 0.803, and 0.196, respectively. The soil conditions properties of pH value, organic matter content, total nitrogen content, and the amount of available phosphorous indices in the study sites were 4.3, 10.6%, 0.34% and $0.68mg{\cdot}kg^{-1}$, respectively. Meanwhile, the total amount of exchangeable cations of $Ca^{2+}$, $K^+$, and $Mg^{2+}$ were $16.84cmol_c{\cdot}kg^{-1}$, $6.41cmol_c{\cdot}kg^{-1}$, and $4.26cmol_c{\cdot}kg^{-1}$, respectively. Overall, Viburnum erosum Thunb. populations tend to grow in the strongly acid soil, which has great abundance of soil organic matters, a lot of exchangeable cations, and has higher amount available phosphorous than that of nitrogen.

• Journal of the East Asian Society of Dietary Life
• /
• v.13 no.5
• /
• pp.433-438
• /
• 2003

To select new useful domestic plants with antimicrobial activities, thirty five samples of berry plant were distributed from Plant Diversity Research Center in Korea Research Institute of Bioscience and Biotechnology. Their extracts with methanol were tested against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Listeria monocytogenes by paper disc method. The methanol extracts from Viburnum dilatatum, Viburnum erosum, Aralia continentalis, Camellia japonica, Acer trumcatam, Arasaema takesimense and Rhysocarpus intermedius were effective against S. aureus and the results were as follows: Viburnum erosum 6.5 mm, Viburnum dilatatum and Aralia continentalis 7.0 mm, Rhysocarpus intermedius 8.0 mm, Acer trumcatum 9.0 mm, Camellia japonica and Arisaema takesimense 9.5 mm. And only the methanol extract from Camellia japonica was effective against L. monocytogenes, 7.0 mm. Antimicrobial activities of E. coli and S. typhymurium were not detected. The minimum inhibitory concentration(MIC) of berry of Camellia japonica was examined as 1,250 $\mu\textrm{g}$/mL against S. aureus and as 1,250∼2,500 $\mu\textrm{g}$/mL against L. monocytogenes.

• Korean Journal of Environment and Ecology
• /
• v.24 no.1
• /
• pp.69-77
• /
• 2010

The Jeongdori windbreak forests of Wando island, located between the sea and the land, are very important as ecological succession areas that protect cultivated lands and fishing villages. Among the Jeongdori windbreak forests, the rocky seashore areas are occupied by Sageretia theezans mantle community, and their inner sections by sub-tree layers such as Ligustrum japonicum and Ligustrum obtusifolium. More specifically, the inner sections of the rocky seashore areas were found to be the habitat of Ligustrum japonicum-Quercus variabilis community, Ligustrum jarponicum-Viburnum erosum community, Ligustrum japonicum-Cinnamomum japonicum community, Cinnamomum japonicum-Carpinus tschonoskii community, and Pinus densiflora-Viburnum erosum community. The average height of tree layers of the Jeongdori windbreak forests is 10.3m, and the average diameter at breath height are 30.3cm. Though the windbreak forests was artificially made, it is now virtually natural forest and deserves protection for ecological reasons. Designated as a scenic spot 3, it is always in the danger of being damaged by frequent tourist visits. So the Jeongdori windbreak forest needs to be continuous maintenance and management by the National Park Service. Considering of the precious cultural treasures of the famous pebble stones covering Gugyedeung and the naturality of the Jeongdori windbreak forests, the Jeongdori windbreak forests as a scenic spot have to designated as the national monument in future and will preserve by the Cultural Properties Administration.

• Toxicological Research
• /
• v.21 no.4
• /
• pp.279-284
• /
• 2005

We have screened the cytoprotective effect against $H_2O_2$ and $\gamma-ray$ radiation induced oxidative stress from 32 Korean plants. Betula ermani var.saitoana (caulis, leaves), Rosa wichuraiana (caulis), Sorbus commixta (caulis), Weigela florida (leaves), Cirsium rhinoceros (whole plant), and Viburnum erosum (caulis) were found to scavenge 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). As a result, extracts of six plants reduced cell death of Chinese hamster lung fibroblast (V79-4) cells induced by $H_2O_2$ treatment. In addition, these extracts protected cell death of V79-4 cells damaged by $\gamma-ray$ radiation. In addition, these extracts scavenged ROS generated by radiation. Taken together, the results suggest that Betula ermani var. saitoana, Rosa wichuraiana, Sorbus commixta, Weigela florida, Cirsium rhinoceros, and Vibumum erosum protect V79-4 cells against oxidative damage by radiation through scavenging ROS.

• Journal of environmental and Sanitary engineering
• /
• v.5 no.1
• /
• pp.63-88
• /
• 1990

The vegetation of Mt. Joghesan of a provincial park in Cheolanamdo was surveyed over twenty times from July, 1980 to December, 1981. The plants of Mt. Jonghesan consisted of 10 forma, 107 varieties, 597 species, 424 genera and 122 families, and among them were 204 species of esculent plants, 199 species of medicina plants and 30 species of the others oil plants and fiber plants. Evergreen herbs consisted of 12 species, including Coniogramme intermedia, Asplenium sarelii, and Asplenium incisum etc. And Evergreen broad-leaved trees consisted of 22 species, including Thea sinensis, Sasa borealis, and Quercus acuta etc. And evergreen needle-leaved trees consisted of 15 species, including Torreya nacitora, Pinus densiflora, Sciadopitys japonica, and Chamaecyparis obtusa etc. The community of broad-leaved consisted of Quercus spp, Carpinus laxiflora, Sasa borealis, including leading dominant species of Lespedeza maximowiczii, Viburnum erosum, Fraxius rhynchophylla, Viburnum dilatum, Rhus trichocarpa, Zelkova serrata, Miscanthus sinensis, Eragrostis ferrugina, Carex augustinowiczii persicaria filiforme var. neofiliforme, Vicia amoena, Smilax riparia var. ussuriensis, and Aster yomena etc. The vegetation of Seunamsa areas in Mt. Joghesan was favorable but the vegetations of the other areas in the mountain were negligible. The vegetation of Koolmokchi areas which had been much destroyed by forest fires was mostly covered with Quercus spp which are resistant to forest fires. Lindera sericea, Alangium platanifolium var. macrophyllum, Ilex macropoda, Corylopsis coreana, Albizzia julibrssin of old trees, Acer mono, the community of Thea sinensis, Stewartca koreana, Cornus alba, Dryopteris bisstiana, Asplenium incisum, Camptosorus, Lepisorus thunbergianus, gastrodia elata, Cymbidium goeringii, and the community of Persicaria filiforme var. neofiliforme etc. in Mt. Jonhesan are autochthonous flora, and their preservation is required. As the Pinus densiflora forest in Mt. Joghesan which was hewn down by human power has not been restored, Jeopchi areas and Koolmokchi areas in Mt. Joghesan have no Pinnus densiflora trees 700m above the sea level.

• Journal of Environmental Science International
• /
• v.16 no.9
• /
• pp.1027-1037
• /
• 2007

The purpose of this study was to investigate the flora of Mt. Bongrae in Busan from February 2004 to May 2005. The flora in the surveyed area were identified 291 taxa that belonged to 82 families, 201 genera, 251 species, 35 varieties, and 5 forms. Among them, evergreen broad trees were identified 21 taxa, naturalized plants 30 taxa, especial plants decided by Ministry of Environment 24 taxa. By the vegetation of each slope, in northeast slope, it was dominated such as Pinus thunbergii community, Chamaecyparis obtusa community, Amorhpa fruticosa community, Trachelospermum asiaticum var. intermedium-Hedera rhombea community, north-northeast slope, it was distributed both Pinus thunbergii community and board-leaved tree community. In the southwest slope of coast site, it was presented plantation plants, according to altitude distributed such as natural vegetation, evergreen broad trees, warm-temperate plants. In southeast slope, it was to represent secondary vegetation such as Alnus firma community as a forest fire. Underside was mainly to search herbs such as naturalized plants and cruciferous family as a vegetable garden. In northwest slope, it was searched trees such as Pinus thunbergii community, Lindera obtusiloba, Styrax japonca, Prunus sargentii, Aralia elata, Zanthoxylum piperitum, Akebia quinata and herb layer such as Osmunda japonica, Allium thunbergii, Liriope platyphylla, Corydalis ochotensis, Scilla scilloides, Viola orientalis. Around the top, it was distributed such as Rhododendron mucronulatum-Rhododendron schlippenbachii community, Carpinus coreana, Viburnum erosum, Sorbus alnifolia.

• Journal of Environmental Impact Assessment
• /
• v.20 no.2
• /
• pp.151-162
• /
• 2011

The purpose of this study is suggest to restoration model of Pinus thunbergii in Saha-gu, Busan Metropolitan City. The result of this study is summarized as follows; As the results of this study, vegetation restoration model is presented by separating community planting and edge planting. The community planting species of tree layer were Pinus thunbergii and Quercus acutissima and Quercus dentata and Quercus serrata and Quercus alienna and Quercus variabilis. The community planting species of subtree layer were Platycarya strobilacea and Prunus sargentii and Styrax japonica and Eurya japonica and Morus bombycis. The community planting species of shrub layer are Ulmus pavifolia and Ulmus davidiana and Lindera obtusiloba and Elaeagnus macrophylla and Mallotus japonicus and Ligustrum obtusifolium and Sorbus alnifolia and Rhus trichocarpa and Zanthoxylum schinifolium and Rosa wichuraiana and Rhus chinensis and Viburnum erosum and Rhododendron mucronulatum and Rhododendron yedoense and Indigofera pseudotinctoria. And the planting species of edge vegetation are Japanese Angelica and Symplocos chinensis and Pittosporum tobira and Lespedeza maximowiczii and Lespedeza bicolor and Rubus coreanus and Rubus idaeus and Vitis thunbergii and Ampelopsis brevipedunculata and Rosa multiflora. Considering the population of individuals up to layers in each $400m^2$ area, it was composed of 24 in tree layer, 35 in subtree layer, 410 in shrub layer and 34% herb layer in the Pinus thunbergii community. And the average of breast-high area and canopy area was $10,852cm^2$ in tree layer, in subtree layer $1,546cm^2$, in shrub layer $1,158,660cm^2$. The shortest distance between trees was calculated as 2.0m in tree layer, 1.9m in subtree layer.