• 한국어병학회지
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• 제25권3호
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• pp.173-180
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• 2012

비브리오 감염증 (Vibriosis) 은 해산어에서 흔히 발생하는 세균성 감염증으로 어종에 따라 다양한 종이 분리, 발견되어지고 있다. 본 보고에서 병어로부터 TCBS한천배지에서 노란색 colony를 생성하는 비브리오속 세균이 분리되었고, 16S rRNA염기서열분석을 통하여 Vibrio scophthalmi로 확인하였다. 병어의 주요 증상으로 체표 출혈, 복강 내벽 출혈, 심한 장염, 복수 저류가 나타났다. 병리조직학적 관찰을 통하여 비장과 신장의 괴사, 장내 상피조직의 괴사, 탈락, 장의 상피하층인 점막고유층에서의 충, 출혈이 관찰되었다. 본 보고는 극동산 양식뱀장어에서의 V. scophthalmi 감염증례에 대한 첫 보고이다.

• Fisheries and Aquatic Sciences
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• 제15권3호
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• pp.233-239
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• 2012

Hematological analysis can provide crucial information for monitoring the health of fish. However, there is no current information available regarding hematological changes in olive flounder following infection by Vibrio scophthalmi. In this study, hematological and biochemical alterations were determined in olive flounder infected by the high virulence strain (HVS) and low virulence strain (LVS) of V. scophthalmi. Survival in serum, skin mucus, and macrophages of olive flounder was also compared between the HVS and LVS. The results demonstrated that the hematocrit value in infected fish declined from 23.4% at 0 h to 18.0% at 168 h post infection. The total protein concentration in fish infected with the HVS was significantly higher than in fish infected with the LVS and a non-infected control. Lysozyme activity was significantly different between infected and control fish. The HVS survived in serum and cell numbers increased substantially, while cell numbers of the LVS in serum decreased. These changes in hematological characteristics in fish infected by V. scophthalmi can be used as an effective and sensitive index to monitor the physiological and pathological conditions of fish. The survival and reproduction of V. scophthalmi in host serum, skin mucus, and macrophages play a major role in systemic infection and can serve as a virulence indicator for different strains.

• 한국어병학회지
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• 제26권3호
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• pp.207-217
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• 2013

최근 울산광역시 소재의 넙치 양식장에서 체색 흑화, 간 위축, 장관 백탁 등의 증상을 보이며 넙치의 대량 폐사가 빈번히 발생하여, 병원체를 분리하고 감염 특성에 관하여 연구하였다. 2012년 5월 병어로부터 분리한 원인균은 생화학 시험과 16S rRNA, dnaJ gene을 이용한 염기서열 분석을 통해 V. scophthalmi로 동정하였다. 병원성 시험 결과, 본 시험 균주가 $10^6$ CFU/fish에서 75%의 누적 폐사율을 보여 강한 병원성이 확인되었다. V. scophthalmi 감염어는 조직병리학적 병변으로서 간 위축, 장 상피 탈락, 장내 세포 물질 유출 및 장관백탁증 등이 확인되었다.

• 한국어병학회지
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• 제37권1호
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• pp.61-69
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• 2024

2022년 7월부터 11월까지 제주 지역의 다양한 넙치양식장을 대상으로 세균성 질병 검사를 수행하였다. 검사를 진행한 3,146마리 중 세균이 검출되지 않은 1,918마리를 제외한 1,228마리에서 검출된 세균을 동정하였다. 그 결과, 773마리(24.6%)에서 Vibrio spp.가 검출되었으며, 그 중 34.8%가 V. harveyi로 확인되었다. S. parauberis와 Vibrio spp.가 동시에 검출된 넙치는 41마리(1.3%)로 나타났고, E. piscicida와 Vibrio spp.가 같이 검출된 넙치는 23마리(0.7%)로 확인되었다. 특히, 한 개체에서 S. parauberis와 Vibrio spp. (V. scophthalmi) 그리고 E. piscicida가 동시에 검출된 경우를 확인할 수 있었다.

• Fisheries and Aquatic Sciences
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• 제22권7호
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• pp.14.1-14.8
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• 2019

Olive flounder (Paralichthys olivaceus) is the major species developed for aquaculture in South Korea. Over the long history of olive flounder aquaculture, complex and diverse diseases have been a major problem, negatively impacting industrial production. Vibriosis is a prolific disease which continuously damages olive flounder aquaculture. A bacterial disease survey was performed from January to June 2017 on 20 olive flounder farms on Jeju Island. A total of 1710 fish were sampled, and bacteria from the external and internal organs of 560 fish were collected. Bacterial strains were identified using 16 s rRNA sequencing. Twenty-seven species and 184 strains of Vibrio were isolated during this survey, and phylogenetic analysis was performed. Bacterial isolates were investigated for the distribution of pathogenic and non-pathogenic species, as well as bacterial presence in tested organs was characterized. V. gigantis and V. scophthalmi were the dominant non-pathogenic and pathogenic strains isolated during this survey, respectively. This study provides data on specific Vibrio spp. isolated from cultured olive flounder in an effort to provide direction for future research and inform aquaculture management practices.

• 한국수산과학회지
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• 제53권3호
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• pp.432-442
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• 2020

Diagnostic monitoring in Korean rockfish cages was performed to survey the prevalence of pathogens in cultured Korean rockfish Sebastes schlegeli from May 2013 to July 2016. A total of 1,945 fish samples collected from the western (Cheonsu Bay and Heuksando), southern (Tongyeong and Namhae), and eastern coasts (Pohang) of Korea were tested for parasites, viruses, and bacteria. In this study, 1,264 and 334 fishes were infected with Microcotyle sebastis and Clavinema mariae, respectively. The prevalence rates of C. clavinema in fishes from Cheonsu Bay, Heuksando, and Tongyeong were 35.3%, 3.9% and 1.9%, respectively. No C. clavinema infection was detected in cultured rockfish from Namhae and Pohang. Furthermore, bacteria including Photobacterium damselae (8.9%), Photobacterium piscicola (2.3%), Photobacterium spp. (8.9%), Aeromonas salmonicida (1.8%), Aeromonas spp. (0.9%), Vibrio scophthalmi (1.5%), Vibrio spp. (3.3%), Streptococcus iniae (1.2%), and others (8.0%) were detected in 373 of 1,364 fishes. No virus was detected in any fish investigated in this study.

• 한국어병학회지
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• 제33권1호
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• pp.91-95
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• 2020

Fish bacterial diseases have spread and caused serious problem for cultured marine fish in Korea. The important bacterial disease affecting mariculture such as olive flounder (Paralichthys olivaceus) are caused by Edwardsiella tarda, Vibrio scophthalmi and Streptococcus parauberis. For the bacterial disease protection in aquaculture industry, the water treatment is needed in aquaculture system. During the last decades atmospheric pressure non-thermal plasma in contact with liquids have received a lot of attention of environmental and medical application. In this study, we determined the disinfectant effect in seawater and three major fish bacterial disease pathogens by using low frequency plasma treatment. Three fish bacteria (E. tarda, V. schophthalmi, S. parauberis) were not detected within 16 min, 150 min and 270 min of 20 L, 500 L and 1 ton seawater post low frequency plasma treatment, respectively. Three major fish bacterial disease pathogens were not detected within 2 min after the low frequency plasma treatment, suggesting that the low frequency plasma possess disinfectant effectiveness.