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Low frequency plasma disinfectant effect in seawater and three major fish bacterial disease pathogens

저온 대기압 플라즈마를 이용한 해수 및 어류 병원성 세균 3종에 대한 살균소독효과

  • Kim, Soo-Jin (Aquatic Disease Control Division, National Institute of Fishery Science (NIFS)) ;
  • Park, Shin-hoo (Aquatic Disease Control Division, National Institute of Fishery Science (NIFS)) ;
  • Jee, Bo-young (Aquatic Disease Control Division, National Institute of Fishery Science (NIFS)) ;
  • Kim, Yong-jae (Korea Aquatic Biosecurity Technology (KABT)) ;
  • Gwon, Mun-Gyoeng (Aquatic Disease Control Division, National Institute of Fishery Science (NIFS))
  • 김수진 (국립수산과학원 수산방역과) ;
  • 박신후 (국립수산과학원 수산방역과) ;
  • 지보영 (국립수산과학원 수산방역과) ;
  • 김영재 ((주)한국방역기술) ;
  • 권문경 (국립수산과학원 수산방역과)
  • Received : 2020.06.05
  • Accepted : 2020.06.16
  • Published : 2020.06.30

Abstract

Fish bacterial diseases have spread and caused serious problem for cultured marine fish in Korea. The important bacterial disease affecting mariculture such as olive flounder (Paralichthys olivaceus) are caused by Edwardsiella tarda, Vibrio scophthalmi and Streptococcus parauberis. For the bacterial disease protection in aquaculture industry, the water treatment is needed in aquaculture system. During the last decades atmospheric pressure non-thermal plasma in contact with liquids have received a lot of attention of environmental and medical application. In this study, we determined the disinfectant effect in seawater and three major fish bacterial disease pathogens by using low frequency plasma treatment. Three fish bacteria (E. tarda, V. schophthalmi, S. parauberis) were not detected within 16 min, 150 min and 270 min of 20 L, 500 L and 1 ton seawater post low frequency plasma treatment, respectively. Three major fish bacterial disease pathogens were not detected within 2 min after the low frequency plasma treatment, suggesting that the low frequency plasma possess disinfectant effectiveness.

Keywords

References

  1. Ahlfeld, B., Li, Y., Boulaaba, A., Binder, A., Schotte, U., Zimmermann, J.L., Morifll, G., Klein, G. 2015. Inactivation of a foodborne norovirus outbreak strain with nonthermal atmospheric pressure plasma. The American Society for Microbiology. 6: 1-5.
  2. Ryu, Y.H., Uhm, H.S., Park G.S., Choi, E.H. 2013. Sterilization of Neurospora crassa by noncontacted low temperature atmospheric pressure surface discharged plasma with dielectric barrier structure. J. Korean Vacuum Soc. 22: 55-65. https://doi.org/10.5757/JKVS.2013.22.2.55
  3. Curras, M., Magarinos, B., Toranzo, A. E., Romalde, J.L. 2002. Dormancy as survival strategy of the fish pathogen Streptococcus parauberis in the marine environment. Disease of Aquatic Organisms. 52: 129-136. https://doi.org/10.3354/dao052129
  4. Domenech, A., Fernandez-Garayzabal, J.F., Pascual, C., Garca, J.A., Cutuli, M.T., Moreno, M.A., Collins, M.D., Dominquez, L. 1996. Streptococcosis in cultured turbot, Scophthalmus maximus(L.), associated with Streptococcus parauberis. J. Fish Dis., 19: 33-38. https://doi.org/10.1111/j.1365-2761.1996.tb00117.x
  5. Eguia, J.M., Chambers, H.F. 2003. Community-acquired methicillin-resistant staphylococcus aureus: epidemiology and potential virulence factors. Curr Infec Dis Rep, 5(6): 459-466. https://doi.org/10.1007/s11908-003-0087-6
  6. Heinlin, J., Morfill, G., Landthaler, M., Stolz, W., Isbary, G., Zimmermann, J.L. 2010. Plasma medicine: possible applications in dermatology. J. Dtsch Dermatol Ges. 8: 968-976.
  7. Huh, M., Lim, J.S., Kim, B.G. 1998. Denitrification of ammonium nitrogen by ozone for a closed recirculating culture system. The Korean Environmental Sciences Society. 7(4): 493-500.
  8. Jo, M.R., Kim, M.C., Song, C.B. 2006. Development of a rapid diagnostic kit for vibrios associated with the farmed olive flounder Paralichthys olivaceus in Jeju Island. Journal of Fish Pathology F3.
  9. Kang, B.J. 2003. A study on the characteristics of bacteria isolated from cultured flounders Paralichthys olivaceus showing disease symptoms in Jeju Area of Korea (Ph.D. thesis). JeJu National University.
  10. Kang, C.Y., Kang, B.J., Moon, Y.G., Kim, K.Y., Heo, M.S. 2007. Characterization of Streptococcus parauberis isolated from cultured olive flounder, Paralichthys olivaceus in the Jeju island. Journal of Fish Pathology. 20: 109-117.
  11. Kim, K.Y., Paik, N.W., Kim, Y.H., Yoo, K.H. 2018. Bactericidal efficacy of non-thermal DBD plasma on Staphylococcus aureus and Escherichia coli. Journal of Korean Society of Occupational and Environmental Hygiene. 28(1): 61-79. https://doi.org/10.15269/JKSOEH.2018.28.1.61
  12. Kim, M.S., Seo, J.S., Park, M.A., Cho, J.Y., Hwang, J.Y., Gwon, M.G., Jung, S.H. 2010. Antimicrobial resistance of Edwardsiella tarda, Vibrio spp., and Streptococcus spp. Isolated from olive flounder, Paralichthys olivaceus. Journal of Fish Pathology. 23(1): 37-45.
  13. Kim, M.S., Cho, J.Y., Seo, J.S., Jung, S.H., Choi, H.S., Park, M.A. 2012. Distribution of MIC value of antibiotics against Edwardsiella tarda isolated from olive flounder (Paralichthys olivaceus). Journal of Fish Pathology, 25(3): 181-188. https://doi.org/10.7847/jfp.2012.25.3.181
  14. Kim, S.H., Woo, S.H., Lee, S.J., Park, S.I. 2013. The infection characteristics of Vibrio scophthalmi isolated from olive flounder, Paralichthys olivaceus. J. Fish. Pathol., 26(3): 207-217. https://doi.org/10.7847/jfp.2013.26.3.207
  15. Kubota, S. S., Kaige, N., Miyazaki, T., Miyashito, T. 1981. Histopathological studies on edwardsiellosis of tilapias. Natural infection. Bull. Fac. Fish. Mie Univ. 9: 155-165.
  16. Lee, Y.S., Kim, Y.B., Kim, K.S., Han, H.G. 2012. Disinfection properties and variation in the ozone concentration in seawater generated using a low-temperature dielectric barrier discharge plasma reactor. Journal of the Environmental Sciences. 21(10): 1181-1186. https://doi.org/10.5322/JES.2012.21.10.1181
  17. Lunov, O., Zablotskii, V., Churpita, O., Jager, A., Polivka, L., Sykova, E., Dejneka, A., Kubinova, S. 2016. The interplay between biological and physical scenarios of bacterial death induced by non-thermal plasma. Biomaterials. 82: 71-83. https://doi.org/10.1016/j.biomaterials.2015.12.027
  18. Nosenko, T., Shimizu, T., Morfill, G.E. 2009. Designing plasmas for chronic wound disinfection. New Journal of Physics. 11: 1-20.
  19. Park, S.Y. 2019. Antibacterial effects of atmospheric plasma against main foodborne bacteria on the surface of dried filefish(Stephanolepis cirrhifer) fillets. J. Food Hyg. Saf. 34: 178-182. https://doi.org/10.13103/JFHS.2019.34.2.178
  20. Qiao, G., Lee, D.C., Woo, S.H., Li, H., Xu, D.H., Park, S.I. 2012. Microbiological characteristics of Vibrio Scophthalmi isolates from diseased olive flounder Paralichthys olivaceus. The Japanese Society of Fisheries Science. 78: 853-863. https://doi.org/10.1007/s12562-012-0502-8
  21. Song, J.W. 2005. A study on the production of ozonized water for environment improvement by gaseous discharge, Master's D. Dissertation, Yeongnam University, South Korea.