Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지
DOI QR코드

DOI QR Code

Vibrio scophthalmi infection in Japanese eel Anguilla japonica during seawater adaption

해수 순치 중의 뱀장어, Anguilla japonica에서 나타나는 Vibrio scophthalmi 감염증

  • Lee, Nam-Sil (Korea New Strategy Research Center, National Fisheries Research & Development Institute (NFRDI)) ;
  • Kim, Dae-Jung (Korea New Strategy Research Center, National Fisheries Research & Development Institute (NFRDI)) ;
  • Lee, Be-Ik (Korea New Strategy Research Center, National Fisheries Research & Development Institute (NFRDI)) ;
  • Kim, Sin Kwon (Korea New Strategy Research Center, National Fisheries Research & Development Institute (NFRDI)) ;
  • Kim, Myung Suk (Pathology Dicision, NFRDI) ;
  • Kim, Yi Cheong (Aquaculture Industry Division, East Sea Fisheries Research Institute, NFRDI)
  • 이남실 (국립수산과학원 전략연구단) ;
  • 김대중 (국립수산과학원 전략연구단) ;
  • 이배익 (국립수산과학원 전략연구단) ;
  • 김신권 (국립수산과학원 전략연구단) ;
  • 김명석 (국립수산과학원 병리연구과) ;
  • 김이청 (국립수산과학원 동해연구소 해역산업과)
  • Received : 2012.09.10
  • Accepted : 2012.11.12
  • Published : 2012.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Vibriosis is one of the most prevalent fish disease belonging to the genus Vibrio. In present study, Vibrio sp. isolated from Japanese eel was confirmed as Vibrio scophthalmi using analysis of the genomic sequence of 16S rRNA. The major signs were hemorrhage of body surface and inner surface of abdomen, severe enteritis and retention of ascitis. Histopathological examination revealed blood cell degenerations in various organs (gills, liver, spleen, kidney, heart, intestine), exfoliate of intestinal epithelium, and congestion and hemorrhage in intestinal lamina propria. This is the first case report on V. scophthalmi infection in Japanese eel Anguilla japonica.

비브리오 감염증 (Vibriosis) 은 해산어에서 흔히 발생하는 세균성 감염증으로 어종에 따라 다양한 종이 분리, 발견되어지고 있다. 본 보고에서 병어로부터 TCBS한천배지에서 노란색 colony를 생성하는 비브리오속 세균이 분리되었고, 16S rRNA염기서열분석을 통하여 Vibrio scophthalmi로 확인하였다. 병어의 주요 증상으로 체표 출혈, 복강 내벽 출혈, 심한 장염, 복수 저류가 나타났다. 병리조직학적 관찰을 통하여 비장과 신장의 괴사, 장내 상피조직의 괴사, 탈락, 장의 상피하층인 점막고유층에서의 충, 출혈이 관찰되었다. 본 보고는 극동산 양식뱀장어에서의 V. scophthalmi 감염증례에 대한 첫 보고이다.

Keywords

References

  1. Actis, L. A., Tolmasky, M. E. and Crosa, J. H.: Vibriosis. In: Fish Diseases and Disorders, Volume 3: Viral, Bacterial and Fungal Infections, 2nd editon (eds, Woo, P.T.K. and Bruno, D.W.), CABI, Wallingford, UK., pp.570-605, 2011.
  2. Aguirre-Guzmán, G., Ruiz, H. M. and Asxencio, F.: A review of extracellular virulence product of Vibrio species important in disease of cultivated shrimp. Aquaculture Research. 35: 1395-1404, 2004. https://doi.org/10.1111/j.1365-2109.2004.01165.x
  3. Austin, B and Austin, D. A.: Bacterial Fish Pathogens: Diseases of Farmed and Wild Fish, 4th edn. Springer-Praxis Publishing, New York-Chichester. 2007.
  4. Blanch, A.R., Alciana, M., Simón, M. and Jofre, J.: Determination of bacteria associated with reared turbot (Scophthalmus maximus) larvae, J. Appl, Microbiol., 82: 729-734, 1997. https://doi.org/10.1046/j.1365-2672.1997.00190.x
  5. Buller, N.B : Bacteria from Fish and other Aqatic Animals: A Practical Identification Manual. CABI Publishing, Wallingford, UK, 2004.
  6. Campbell, A.C. and Buswell, J. A.: The intestinal microflora of farmed Dover sloe (Solea solea) at different stages of fish development. J. Appl. Bacteriol., 55: 215-223, 1983. https://doi.org/10.1111/j.1365-2672.1983.tb01318.x
  7. Carsen, F.: Histotechnology; A Self-Instructional Text, pp194-196, ASCP, III, 1990.
  8. Cerda-Cuellar, M. and Blanch, A. R.: Detection and identification of Vibrio scophthalmi in the intestinal microbiota of fish and evaluation of host specificity. J. Appl. Microbiol., 93: 261-268, 2002. https://doi.org/10.1046/j.1365-2672.2002.01697.x
  9. Cerda-Cuellar, M., Ramon A., Rossello-Mora, Lalucat J., Jofre, J., and Blanch, A.: Vibrio scophthalmi sp. nov., a New species from Turbot (Scophthalmus maximus).Int. J. Syst. bacteriol., 47: 58-61, 1997. https://doi.org/10.1099/00207713-47-1-58
  10. Cerda-Cuellar, M. and Blanch, A. R.: Determination of Vibrio scophthalmi and its phenotypic diversity in turbot larvae. Environ. Microbiol. 6: 209-217, 2004. https://doi.org/10.1046/j.1462-2920.2004.00555.x
  11. Clarridge, J. E.: Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clinic. Microbiol. Reviews, 17: 840-862, 2004. https://doi.org/10.1128/CMR.17.4.840-862.2004
  12. Fouz, B., Larsen, J. L., Nielsen, B. Barja, J. L. and Toranzo, A. E.: Characterization of Vibrio damsela strains isolated from turbot Scophthalus maximus in Spain. Dis. Aquat. Org. 12: 155-166, 1992. https://doi.org/10.3354/dao012155
  13. Frans, I., Michiels, C. W., Bossier, P., Wikkems, K.,A., Lievens, B. and Rediers, H.: Vibrio anguillarum as a fish pathogen: virulence factors, diagnosis and prevention. J. Fish Dis. 34: 643-661, 2011. https://doi.org/10.1111/j.1365-2761.2011.01279.x
  14. Frans, R., Sylvia, P.-V., Sorja, K. Annemiek, B., Maselon, W., Ton, N. and Loga, H.: Development of a polymerase chain reaction for the detection of anguillid herpesvirus DNA in eels based on the herpesvirus DNA polymerase gene. J. Virol. methods, 124: 87-94, 2005. https://doi.org/10.1016/j.jviromet.2004.11.007
  15. Grisez L., Chair, M. Sorgeloos, P. and Ollevier, F.: Mode of infection and spread of Vibrio anguillarum in tutbot Scophthalmus maximus larvae after oral challenge through live feed. Dis. Aquat. Org. 26: 181-187, 1996. https://doi.org/10.3354/dao026181
  16. Hosono, N., Suzuki, S. and Kusuda, R.: Genogrouping of birnavirus isolated from marine fish: a comparison of VP2/NS junction regions on genome segment A. J. Fish Dis. 19: 295-302, 1996. https://doi.org/10.1111/j.1365-2761.1996.tb00707.x
  17. McCarthy, D. H.: Vibrio disease in eels. J. Fish Biol. 8: 317-320, 1976. https://doi.org/10.1111/j.1095-8649.1976.tb03955.x
  18. Muroga, K., Higashi, M., and Keitoku, H.: The isolation of intestinal microflora of farmed red seabream (Pagrus mahor) and black seabream (Acanthopagrus schlegeli) at larval and juvenile stages. Aquaculture 65: 79-88, 1987. https://doi.org/10.1016/0044-8486(87)90272-9
  19. Myhr, E., Larsen, J. L., Lillehaug, A., Gudding, R., Heum, M. and Hastein, T.: Characterization of vibrio anguillarum and closely related species isolated from farmed fish in Norway. Appl. Environ. Microbiol. 57: 2750-2757, 1991.
  20. Nicolas, J. L., Robic, E., and Ansquer, D.: Bacterial flora associated with trophic chain consisting of microalgae, rotifers and turbot larvae: influence of bacteria on larval survival . Aquaculture 83: 237-248, 1989. https://doi.org/10.1016/0044-8486(89)90036-7
  21. Pillard, C., Lerous, F. and Borrego, J. J.: Bacterial disease in marine bivalves: review of recent studies: trends and evolution. Aqua. Living Resources, 17: 477-498, 2004. https://doi.org/10.1051/alr:2004054
  22. Ramzi, S. C., Vinay, K. and Tucker, C.: Robbins pathologic basis of disease. sixth edition. Chap.19. p.855, 1999.
  23. Richards, W. L (ed.) : A diagnostic manual of eel disease occuring under culture condition in Japan. Sea Grant Publication UNC-SG-78-06 UNC Sea Grant Raleigh, 1978.
  24. Snow, M., Bain, N., Black, J., Taupin, V., Cunnigham, C. O., King, J. A., Skall, H. F. and Raynard, R. S.: Genetic population structure of marine viral haemorrhagic septicaemia virus (VHSV).Dis. Aquat. Org. 61: 11-21, 2004. https://doi.org/10.3354/dao061011
  25. Steven, J. B., Michal A. V-L., Ineke, R., Annette, S. B., Olga, L. M. and Marc. Y. E.: Development and validation of a two-step real-time RT-PCR for the detection of eel virus European X in European eel, Anguilla anguilla, J. Virol. Methods, 171: 352-359, 2011. https://doi.org/10.1016/j.jviromet.2010.11.019
  26. Sun, Y., Yue, Z., Liu, H., Zhao, Y., Liang, C., Li, Y., Shi, X., Wu, B., Deng, M., Zhu, L. and Wang, Z.: Development and evaluation of a sensitive and quantitative assay for hirame rhabdovirus based on quantitative RT-PCR. J. Virol. Methods, 169: 391-396, 2010. https://doi.org/10.1016/j.jviromet.2010.08.011
  27. Tanasomwang, V., and Muroga, K. Intestinal microflora of larval and juvenile stages in Japanese flounder (Paralichthys olivaceus).Fish Pathol. 23: 77-83, 1988. https://doi.org/10.3147/jsfp.23.77
  28. Tetsuya, M., Yusuke, S., Akira, N., Hideharu, O., Kouji, S., Kouji, W,. Nozomi, T., Emi, M., Mami, O., Ichiro, K., Masayuki, S., Shigeru, M. and Sjin-ichi, O.: Novel DNA virus isolated from samples showing endothelial cell necrosis in the Japanese eel, Anguilla japonica, Virology 312: 179-187, 2011
  29. Toranzo, A. E., Magarinos D. and Romalde, K. L.: A review of the main bacterial fish diseases in mariculture systems, Aquaculture 246: 37-6, 2005. https://doi.org/10.1016/j.aquaculture.2005.01.002
  30. Westerdahl, A., Olsson, J. C., Conway, P. L. and Kjelleberg, S.: Characterization of turbot (Scophthalmus maximus) associated bacteria with inhibitory effects against the fish pthogen Vibrio anguillarum. Aca. microbio.l Immunol. Hung. 41:403-409, 1994.
  31. Wakabayashi, H. and Egusa, S.: Characteristics of a Pseudomona sp. from an epizootic of pond cultured eel (Anguilla japonica). Bull. Japan. Soc. Scient. Fish, 38: 577-587, 1972. https://doi.org/10.2331/suisan.38.577
  32. Wiik, R., Stackebrandt, E., Valle, O., Daae, F. L., Rodseth, O. M. and Andersen, K.: Classification of fish-pathogenic vibrio based on cpmparative 16S rRNA analysis. Int. J. Systematic Bacteriol., 45: 421-428, 1995. https://doi.org/10.1099/00207713-45-3-421

Cited by

  1. Complete genome analysis of a virulent Vibrio scophthalmi strain VSc190401 isolated from diseased marine fish half-smooth tongue sole, Cynoglossus semilaevis vol.20, pp.1, 2012, https://doi.org/10.1186/s12866-020-02028-7