• 한국패류학회지
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• 제31권4호
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• pp.257-262
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• 2015

The seven selected primers BION-13, BION-29, BION-61, BION-64, BION-68, BION-72 and BION-80 generated the total number of loci, average number of loci per lane and specific loci in Meretrix lusoria (ML), Saxidomus purpuratus (SP) and Cyclina sinensis (CS) species. Here, the complexity of the banding patterns varied dramatically between the primers from the three venerid clam species. The higher fragment sizes (> 1,000 bp) are much more observed in the SP species. The primer BION-68 generated 21 unique loci to each species, which were ascertaining each species, approximately 150 bp, 300 bp and 450 bp, in the ML species. Remarkably, the primer BION-80 detected 7 shared loci by the three clam species, major and/or minor fragments of sizes 500 bp, which were matching in all samples. As regards average bandsharing value (BS) results, individuals from CS clam species (0.754) exhibited higher bandsharing values than did individuals from SP clam species (0.607) (P < 0.05). In this study, the dendrogram obtained by the seven oligonucleotides primers indicates three genetic clusters: cluster 1 (LUSORIA01-LUSORIA07), cluster 2 (PURPURATUS08-PURPURATUS14), cluster 3 (SINENSIS15-SINENSIS21). Among the twenty one venerid clams, the shortest genetic distance that displayed significant molecular differences was between individuals 18 and 20 from the CS species (genetic distance = 0.071), while the longest genetic distance among the twenty-one individuals that displayed significant molecular differences was between individuals LUSORIA no. 02 and PURPURATUS no. 09 (genetic distance = 0.778). Relatively, individuals of SP venerid species were appropriately closely related to that of CS species, as shown in the hierarchical dendrogram of genetic distances. Eventually, PCR fragments exposed in the present study may be worthwhile as a DNA marker the three venerid clam species to discriminate.

• Ocean and Polar Research
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• 제32권3호
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• pp.247-254
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• 2010

Single cell PCR analysis and light and scanning electron microscopic techniques were utilized to identify free living bivalve larvae in the coastal waters of Tae-an, on the west coast of Korea. Through DNA sequencing, venerid clam larvae were isolated and identified as Ruditapes philippinarum (99% similarity) and Meretrix lusoria (99%). Under microscopic observation, the D-veliger stage of R. philippinarum exhibited symmetrical shoulder angles and an elliptical ventral form. In contrast, M. lusoria displayed asymmetrical shoulder angles and a round ventral form in the umbonal stage. Size of the R. philippinarum larvae was $156{\pm}22{\mu}m$ in length, $126{\pm}12{\mu}m$ in height, $92{\pm}14{\mu}m$ in width with a length: height ratio of 1.23. Meretrix lusoria was $202{\pm}44{\mu}m$ in length, $161{\pm}35{\mu}m$ in height, $96{\pm}38{\mu}m$ in width with a length: height ratio of 1.25. Experimental results indicate that morphological and molecular characteristics provide evidence for the larval identification of these two venerid clam larvae species in nature.

• 한국양식학회지
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• 제20권2호
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• pp.75-80
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• 2007

백합과(科)에 속하는 유용패류인 살조개, 바지락, 백합, 개조개 및 가무락조개 등 5종간의 아미노산 조성과 그에 따른 유연관계를 밝히고자 하였다. 백합과(科) 5종의 주요아미노산 구성은 ureanine, taurine, proline, glycine, alanine, arginine 등으로 나타났다. 백합과(科) 5종간 아미노산 함량으로 유사도를 분석한 결과 살조개와 바지락간에는 0.89, 가무락조개와 백합간에는 0.94, 개조개는 살조개와 바지락간에 0.88을 보였으며, 가무락조개와 백합, 살조개, 바지락 및 개조개간에는 0.52의 유사도를 보였다.

• 한국양식학회지
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• 제17권4호
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• pp.251-257
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• 2004

상업적 가치가 높고 양식 가능성이 있는 백합과 5종을 대상으로 RAPD방법을 이용한 개체간의 유전적 유연관계를 조사하였다. DNA 추출은 protein K-phenol방법을 사용하여 각 종의 후폐각근에서 추출하였다. RAPD-PCR결과 15개의 primer들이 증폭되었고, 그들로부터 각각 1-3개의 band를 볼 수 있었다. 백합과 5종간 유전적 유사도는 살조개와 바지락간에는 0.84, 개조개와 백합간에는 0.87로 각각의 두 종간에는 놓은 유사도를 보였고, 가무락조개는 살조개와 바지락간에 0.78의 유사도를 보였으며, 개조개와 백합, 살조개, 바지락, 가무락조개간에는 0.46의 비교적 낮은 유전적 유사도를 보였다. RAPD 방법으로 종내개체 유전변이를 파악하기는 힘들어도, 양식이나 자원증식을 위한 패류의 우수형질 선택에 있어 기본 정보를 제공 할 수는 있을 것이다. 또한, 살조개 대량종묘생산의 방법은 유전적 유사도가 가장 가까운 바지락을 중심으로 이루어져야 할 것으로 사료된다.