Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Genetic Relationship of the Five Venerid Clams유 (Bivalvia, Veneridae) in Korea

한국산 백합과 5종의 유전적 유연관계

  • 정형택 (여수대학교 수산생명과학부) ;
  • 김정 (수산증양식 연구센터) ;
  • 신종암 (여수대학교 수산생명과학부) ;
  • 서호영 (여수대학교 수산생명과학부) ;
  • 최상덕 (여수대학교 수산생명과학부)
  • Published : 2004.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

The random amplified polymorphic DNAs (RAPD) technique was used to characterize the genetic relationship of five species from in the family of Veneridae which is one of the commercially important clam family in Korea. The veneride clams' DNA were extracted from adductor muscular by the proteinase K-phenol method. Among 20 primers, 15 unit primers were amplified and produced at least, 2 or 3 from the top band. Genetic similarity between the purplish washington, Saxidomus purpuratus and the hard clam, Meretrix lusoria was the highest (0.87); the lowest genetic similarity (0.46) was formed between the little clam, Ruditapes philppinarum and the purplish washington, S. purpuratus. The genetic relationship between the venus clam, Protothaca jedoensis and the little clam, R. philppinarum was a closer than those between others. These results may indicate that the method of artificial seeding production of P. jedoensis for the propagation of resources can be focused on R. philppinarum.

상업적 가치가 높고 양식 가능성이 있는 백합과 5종을 대상으로 RAPD방법을 이용한 개체간의 유전적 유연관계를 조사하였다. DNA 추출은 protein K-phenol방법을 사용하여 각 종의 후폐각근에서 추출하였다. RAPD-PCR결과 15개의 primer들이 증폭되었고, 그들로부터 각각 1-3개의 band를 볼 수 있었다. 백합과 5종간 유전적 유사도는 살조개와 바지락간에는 0.84, 개조개와 백합간에는 0.87로 각각의 두 종간에는 놓은 유사도를 보였고, 가무락조개는 살조개와 바지락간에 0.78의 유사도를 보였으며, 개조개와 백합, 살조개, 바지락, 가무락조개간에는 0.46의 비교적 낮은 유전적 유사도를 보였다. RAPD 방법으로 종내개체 유전변이를 파악하기는 힘들어도, 양식이나 자원증식을 위한 패류의 우수형질 선택에 있어 기본 정보를 제공 할 수는 있을 것이다. 또한, 살조개 대량종묘생산의 방법은 유전적 유사도가 가장 가까운 바지락을 중심으로 이루어져야 할 것으로 사료된다.

Keywords

References

  1. Avise, J. C, 1994. Molecular markers, natural history and evolution. Chapman and Hall, New York, 511 pp
  2. Baradakci, F. and D. O. F. Skibinski, 1994. Application of the RAPD technique in tilapia fish: Species and subspecies identification. Heredity, 73: 117-123 https://doi.org/10.1038/hdy.1994.110
  3. Caetano-Anolles, G., B. J. Bassam and P. M. Gresshoff, 1991. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Bio. Tech., 9: 553-557 https://doi.org/10.1038/nbt0691-553
  4. Chee, H. Y., Y. K. Kim and Y. J. Park, 2001. Genetic relationship among three scallop species, Chlamys farreri farreri, Patinopecten yessoensis and Agropecten irradians, using RAPD markers. J. Malacol, 17(1): 1-6
  5. Cho, Y. C., J. W. Park., H. J. Jin., B. H. Nam., C. H. Sohn and Y. K. Hong, 1997. RAPD identification of genetic variation in ulvales seaweed. J. Korean Fish. Soc., 30(3): 388-392
  6. Dutcher, J. A. and D. F. Kapraum, 1994. Random amplified polymorphic DNA (RAPD) identification of genetic variation in three species of Porphyra (Bangiales: Rhodohyta). J. Appl. Phycol., 6: 267-273 https://doi.org/10.1007/BF02181937
  7. Fekete, A., J. A. Bantle., S. M. Halling and R. W. Stich, 1992. Amplification fragment length polymorphism in Brucella strains by use of polymerase chain reaction with arbitrary primers. J. Bacteriol., 174: 7778-7783 https://doi.org/10.1128/jb.174.23.7778-7783.1992
  8. Goodwin, P. H. and S. L. Annis, 1991. Rapid identification of genetic variation and phenotype of Leptosphaeria maculans by ramdom amplified polymorphic DNA assay. Appl. Environ. Microbiol., 57: 2482-2486
  9. Hadrys, H., M. Balick and B. Schierwater, 1992. Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Mol. Ecol., 1: 55-63 https://doi.org/10.1111/j.1365-294X.1992.tb00155.x
  10. Ho, C. L., S. M. Phang and T. Pang, 1995. Molecular characterization of Sargassum polycystum and S. siliquosum (Phaeophyta) by polymerase chain reaction DNA (RAPD) primers. J. Appl. Phycol., 7: 33-42 https://doi.org/10.1007/BF00003547
  11. Hong, Y. K., S. D. Kim., M. Polne-Fuller and A. Gibor, 1995. DNA extraction conditions from Porphyra peiforata using LiCI. J. Appl. Phycol., 7: 101-107 https://doi.org/10.1007/BF00693055
  12. Jackson, D. P., J. D. Hayden and P. Quirke, 1991. Extraction of nucleic acid from fresh and archival material. In PCR: A practical approach, M. J. Mcpherson, P. Quirke and G. R. Taylor, eds. IRL Press, New York, pp. 1-49
  13. Kim, J., 2002. Studies on the phylogenetic relationship and reproductive cycle of the venus clam, Protothaca jedoensis in Korea, Ph.D thesis. Yosu Nat'I Univ. 169 pp
  14. Kim, J., H. S. Yoon., S. J. Rha., S. Y. Moon., H. Y. Soh., K. J. Choi and S. D. Choi, 2002. Reproductive cycle of venus of clam, Protothaca jedoensis (Bivalvia: Veneridae) in Korea J. Environ. Biology, 20(3): 245-255
  15. Klinbunga, S., Ampayup, P., Tassanakajon, A., Jarayabhand, P. and W. Yoosukh, 2000. Development of species-specific markers of the tropical oyster (Crassostrea belcheri) in Thailand. Mar. Biotechnol, 2: 476-484
  16. Kwon, O. K., K. M. Park and J. S. Lee, 1993. Coloured Shells of Korea. Academy Publishing Co., Seoul, 371 pp
  17. Lee, J. M., J. W. Park., M. S. Yoo and Y. G. Hong, 1997. Morphological characters and genetic diversity using the RAPD technique in the arkshell, Scapharaca broughtonii (Schrenck) from Korea and China. J. Korean Fish. Soc., 30: 297-304
  18. Lee, J. M., 2000. Morphology, genetic characters and reproductive cycle of local populations in the arkshell, Scapharca broughtonii (Schrenck) (Pelecypoda: Arcidae) from Korea, Ph.D thesis. Pukyong Nat'l Univ. 120 pp
  19. Lim, H. S., J. W. Choi., J. G. Je and J. H. Lee, 1992. Distribution pattern of macrozoobenthos at the farming ground in the western part of Chinhae Bay. Korea. Bull. Korean Fish. Soc., 25: 115-132
  20. Magurram, A. E., 1988. Ecological diversity and its measurement. Croom Helm, London, 179 pp
  21. MOMAF., 2000. Genetic analysis and data management program development of major fishery species in Korea. MOMAF, 283 pp
  22. Nei, M., 1987. Molecular evolutionary genetics. Columbia University Press, New York, 512 pp
  23. O'Reilly, P. and J. M. Wright, 1995. The evolving technology of DNA fingerprinting and its application to fisheries and aquaculture. J. Fish BioI., 47(suppl. A): 29-55 https://doi.org/10.1111/j.1095-8649.1995.tb06042.x
  24. Parker, M. S., 2000. Population genetics of Protothaca staminea and Nacoma balthica in Puget Sound. WA. J. Shellfish Res., 19(1): 686-705
  25. Patwary, M. U., R. M. Mackray and J. P. Vander Meer, 1993. Revealing genetic markers in Gelidium vagum (Rhodophyta) through the random amplified polymorphic DNA (RAPD) technique. J. Phycol., 29: 216-222 https://doi.org/10.1111/j.0022-3646.1993.00216.x
  26. Patwary, M. U., E. L. Kenchington., C. J. Bird and E. Zouros, 1994. The use of random amplified polymorphic DNA markers in genetic studies of the sea scallop, Placopeeten magellanicus. J. Shellfish Res., 13: 547-553
  27. Sneath, P. H. and P. R. Sokal, 1973. Numerical taxonomy. Freeman, San Francisco, 635 pp
  28. Sogin, M. L., 1990. Amplification of ribosomal RNA genes for molecular revolution studies. In PCR protocolos; A guide to methods and applications. M.A. Gelfane. D.H. Sninsky and T.J. White. eds. Academic Press. New York. pp. 307-314
  29. Sundberg, P. and S. Andersson, 1995. Random amplified polymorphic DNA (RAPD) and intraspecific variation in Oerstedia dorsalis (Hoplonemertea:Nemertea). J. Mar. BioI. Ass, U.K., 75: 483-490 https://doi.org/10.1017/S0025315400018324
  30. Tingey, S. V. and J. P. del Tufo, 1993. Genetic analysis with random amplified polymorphic DNA markers. Plant Physiol., 101: 349-352 https://doi.org/10.1104/pp.101.2.349
  31. Williams, J. G. K., A. R. Kugelik., K. J. Livak., J. A. Rafolski and S. V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 6351-6535
  32. Williams, J. G. K., M. K. Hanafey., J. A. Rafolski and S. V. Tingey, 1993. Genetic analysis using random amplified polymorphic DNA markers. Methods Enzymol, 218: 704-740 https://doi.org/10.1016/0076-6879(93)18053-F
  33. Yokogawa, K., 1997. Morphological and genetic differences between Japanese and Chinese red arkshell, Anadara broughtonii. Fish. Sci., 63: 332-337 https://doi.org/10.2331/fishsci.63.332
  34. Yoo, J. S., 1976. Korean Shells in Colour. II Ji Sa Publish. Co., Seoul
  35. Yoo, S. K., 1970. Biological studies on the propagation of important bivalves. 2. Growth and morphological variations of Anadara broughtonii(Schrenck). Bull. Pusan Fish. Coli, 10: 81-89
  36. Yoon, J. M., K. H. Park and S. N. Choe, 2003. Genetic polymorphism of Marsh Clam (Corbicula Leana) identified by RAPDPCR. J. Fish. Sci. Tech, 6(1): 13-19 https://doi.org/10.5657/fas.2003.6.1.013