• Molecular & Cellular Toxicology
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• v.1 no.3
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• pp.172-178
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• 2005

[ $21{\alpha}$ ]- and ${\beta}$-Methylmelianodiol were isolated as the inhibitor of IL-5 bioactivity from Poncirus tripoliata. To develope as an anti-septic drug, the genotoxicity of $21{\alpha}\;-and\;{\beta}-methylmelianodiol$ was subjected to high throughput toxicity screening (HTTS) because they revealed strong IL-5 inhibitory activity and limitation of quantity. Mouse lymphoma thymidine kinase ($tk^{+/-}$) gene assay (MOLY), single cell gel electrophoresis (Comet) assay in mammalian cells and Ames reverse mutation assay in bacterial system were used as simplified, inexpensive, short-term in vitro screening tests in our laboratory. These compounds are not mutagenic in S. typhimurium TA98 and TA100 strains both in the presence and absence of metabolic activation. Before performing the comet assay, $IC_{20}$ of $21{\alpha}-methylmelianodiol$ was determined the concentration of $25.51\;{\mu}g/mL\;and\;21.99\;{\mu}g/mL$ with and without S-9, respectively. Also $21{\beta}-methylmelianodiol$ was determined the concentration of $24.15\;{\mu}g/mL\;and\;\;22.46\;{\mu}g/mL$ with and without S-9, respectively. In the comet assay, DNA damage was not observed both $21{\alpha}-methylmelianodiol\;and\;21{\beta}-methylmelianodiol$ in mouse lymphoma cell line. Also, the mutant frequencies in the treated cultures were similar to the vehicle controls, and none of $21{\alpha}\;-and\;{\beta}-methylmelianodiol$ with and without S-9 doses induced a mutant frequency over. twice the background. It is suggests that $21{\alpha}\;-and\;{\beta}-methylmelianodiol$ are non-mutagenic in MOLY assay. The results of this battery of assays indicate that $21{\alpha}\;-and\;{\beta}-methylmelianodiol$ have no genotoxic potential in bacterial or mammalian cell systems. Therefore, we suggest that $21{\alpha}\;-and\;{\beta}-methylmelianodiol$, as the optimal candidates with both no genotoxic potential and IL-5 inhibitory effects must be chosen.

• Journal of the Korean Geographical Society
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• v.30 no.4
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• pp.352-363
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• 1995

This study explores horizontal equity problems in the Seoul Subway System by using costs per passenger kilometer as a comparison index. In general, subway users in the central area, except Line 1, are subsidizing users in fringe areas. Spatial differentiation of user cross subsidies in the area is due mainly to interactions between factors such as vehicle kilometers traveled, number of passengers, and length of segments. In order to decrease levels of user cross subsidies among the users and so relieve honzontal equity problems associated with subway use, it is necessary to develop a user fare system in which portions of marginal costs of subway use can be distributed properly among users. However, two-stage fare structure of the subway system in the study area is based only on trip distance, and so marginal costs associated with subway use are not properly reflected in user fees. Therefore, horizontal equity of the system in the study area is affected because of inappropriate user fees.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.45 no.6
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• pp.26-34
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• 2008

The information of traversability and path planning is essential for UGV(Unmanned Ground Vehicle) navigation. Such information can be obtained by analyzing 3D terrain. In this paper, we present the method of 3D terrain modeling with color information from a camera, precise distance information from a 2D Laser Range Finder(LRF) and wheel encoder information from mobile robot with less data. And also we present the method of 3B terrain modeling with the information from GPS/IMU and 2D LRF with less data. To fuse the color information from camera and distance information from 2D LRF, we obtain extrinsic parameters between a camera and LRF using planar pattern. We set up such a fused system on a mobile robot and make an experiment on indoor environment. And we make an experiment on outdoor environment to reconstruction 3D terrain with 2D LRF and GPS/IMU(Inertial Measurement Unit). The obtained 3D terrain model is based on points and requires large amount of data. To reduce the amount of data, we use cubic grid-based model instead of point-based model.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.4
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• pp.897-905
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• 2015

This research suggested the traffic signal calculation model of active transit signal priority using a shockwave model. Using this signal priority timing optimization model, the shockwave area is computed under the condition of Early Green and Green Extension among active transit signal priority techniques. This study suggested the speed estimation method of backward shockwave using average travel time and intersection passing time. A shockwave area change is calculated according to signal timing change of transit signal priority. Moreover, this signal timing calculation model could determine the optimal signal priority timings to minimize intersection delay of general vehicles. A micro simulation analysis using VISSIM and its user application model ComInterface was applied. This study checked that this model could calculate the signal timings to minimize intersection delay considering saturation condition of traffic flow. In case studies using an isolated intersection, this study checked that this model could improve general vehicle delay of more over ten percentage as compared with equality reduction strategy of non-priority phases. Recently, transit priority facilities are spreading such as tram, BRT and median bus lane in Korea. This research has an important significance in that the proposed priority model is a new methodology that improve operation efficiency of signal intersection.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.1D
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• pp.13-22
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• 2012

The 119 emergency rescue-aid service operated by Korean government is a very valuable in a society and its importance is growing in Korea as an aging society. Especially, the emergency vehicle's arrival time to accidents place is an important variable which affects initial emergency measure for patients and it depends on the road network attributes, such as emergency service station's location, accessibility to accidents place and so on. This study aims to analysis the emergency vehicles' arrival delay and the dispatch station in the viewpoint of efficiency utilizing the real rescue-aid activity data. We analyzed the dispatch distribution of the emergency rescue-aid service at first. And we analyzed high accident rate locations not involved in the fixed radius of rescue-aid service stations and display GIS map showing regions have been delayed. The input data of the road network speed is based on the KTDB (Korea Transportation Database) and historical rescue-aid data is from Gyeongsangbuk-do's fire service headquarters.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.38 no.8
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• pp.665-670
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• 2014

Because reducing atmospheric pollution is becoming a serious issue, studies are actively focusing on exhaust gas reduction. This study was conducted to determine the emission characteristics when applying an EGR system, the main approach used for NOx reduction, to an off-road mechanical diesel engine. For the application of the EGR system, the emission characteristics in consideration of the engine conditions were analyzed. The optimum EGR ratio for NOx emission reduction was determined by applying variable EGR conditions for each engine speed condition. Considering the above process, the emission characteristics of the modified EGR condition are compared with those of other conditions (non-EGR and existing EGR condition) in the NRTC mode. Consequently, NOx emission was reduced by around 42 compared with the non-EGR condition when using the modified EGR map.

• Development and Reproduction
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• v.4 no.1
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• pp.53-59
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• 2000

Endocrine disruptors (EDs) are exogenous chemicals which interfere several aspects of natural hormone properties. EDs with estrogenic activity have been recently reported to cause animal reproductive problems. This study was performed to investigate the effects of bisphenol A (BPA) on the mouse spermatogenesis in vivo. Male ICR mice were orally injected on a daily basis with low dose of BPA 20 mg/kg, high dose of BPA 200 mg/kg, or corn oil (vehicle control) for 7 days, and litter size and weights of body, testis, and cauda epididymis were measured. The level of serum testosterone and the expression of TGF- $\beta$$_1$ mRNA were also analyzed using RIA and RT-PCR, respectively. Also, morphological differences of testes after treatments were examined. Sperm concentration and level of serum testosterone showed a decreasing tendency detected as untreated >corn oil >low >high dose BPA treated mice, although there were no significant statistical differences. Interestingly, in mice treated with a high dose of BPA, partial disappearance of spermatozoa in seminiferous tubular lumen and the expression of TGF-$\beta$$_1$ mRNA were observed. Spermatogenesis was disrupted through TGF-$\beta$ system in the seminiferous tubules, resulting in no development of germ cells. Similarly, the litter size treated with a high dose of BPA was significantly different from that of untreated control group. In conclusion, these results that a high dose of BPA (200 mg/kg) acts as an endocrine disruptor during apermatogenesis in male mice md that there are BPA-specific lesions in the adult male reproductive tract might represent a permanently altered responsiveness to testosterone by BPA in the affected target tissue.

• Molecules and Cells
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• v.19 no.2
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• pp.191-197
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• 2005

The human immunodeficiency virus type 1 (HIV-1) Tat protein transduction domain (PTD) is responsible for highly efficient protein transduction across plasma membranes. In a previous study, we showed that Tat-Cu,Zn-superoxide dismutase (Tat-SOD) can be directly transduced into mammalian cells across the lipid membrane barrier. In this study, we fused the human SOD gene with a Tat PTD transduction vector at its N- and/or C-terminus. The fusion proteins (Tat-SOD, SOD-Tat, Tat-SOD-Tat) were purified from Escherichia coli and their ability to enter cells in vitro and in vivo compared by Western blotting and immunohistochemistry. The transduction efficiencies and biological activities of the SOD fusion protein with the Tat PTD at either terminus were equivalent and lower than the fusion protein with the Tat PTD at both termini. The availability of a more efficient SOD fusion protein provides a powerful vehicle for therapy in human diseases related to this anti-oxidant enzyme and to reactive oxygen species.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.46 no.1
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• pp.1-9
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• 2018

Lambda wing type Unmanned Combat Aerial Vehicle(UCAV) which adopts Blended Wing Body(BWB) has relatively less drag and more stealth performance than conventional aircraft. However, Pitching moment is rapidly increased at a specific angle of attack affected by leading edge vortex due to leading edge sweep angle. Wind tunnel testing and numerical analysis were carried out with UCAV 1303 configuration on condition of 50 m/s of flow velocity, $-4^{\circ}{\sim}28^{\circ}$ of the range of angle-of-attack. The effect of wing twist for longitudinal stability at the various angles of attack was verified in this study. When negative twist is applied on the wing, Pitch-break was onset at higher angle of attack due to delayed flow separation on outboard of the wing. On the other hand, pitch-break was onset at lower angle of attack and lift-to-drag ratio was increased when positive twist is applied on the wing.

• Journal of the Korean Institute of Gas
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• v.18 no.1
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• pp.46-51
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• 2014

The purpose of this paper is to study of the vehicle lamp manufacturing system composing ultrasonic waves connection process. Making lamp assembly plant, it was produced in the separate process as the injection molding, ultrasonic waves bonding, annealing in the constant temperature, lamp assembling and packing. But the improvement method producing the lamp was added with one-step process by one automation technique. As a result, welding with ultrasonic waves process, the method decreased the energy consumption and noise during ultrasonic waves welding. Therefore, this method used the mathematics modeling for checking validity, it selected the stability and suitable controller using transfer function of plant and bode chart. In this study, the $180^{\circ}$ revolution control system to turn injection part upside down was $M_{eq}\;lcos{\theta}(t)$ because of gravity influence. It effected to unstable condition a system. For solving this problem, it aimed the linearization and stabilization of system by elimination $M_{eq}\;lcos{\theta}(t)$ as applying Free-forward control technique.