• Journal of Microbiology and Biotechnology
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• v.24 no.1
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• pp.70-79
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• 2014

In an attempt to develop a variety of expression vector systems for Corynebacterium glutamicum, six types of promoters, including $P_{tac}$, $P_{sod}$, $P_{sod}$ with a conserved Shine-Dalgarno (SD) sequence from C. glutamicum, $P_{ilvC}$, $P_{ilvC}$ with a conserved SD-1 ($P_{ilvC-M1}$), and $P_{ilvC}$ with a conserved SD-2 ($P_{ilvC-M2}$), were cloned into a modified shuttle vector, pCXM48. According to analysis of promoter strength by quantitative reverse transcription PCR, $P_{sod}$ and $P_{sod-M}$ were superior to tac and ilvC promoters in terms of transcription activity in C. glutamicum. All of the promoters have promoter activities in Escherichia coli, and $P_{sod-M}$ displayed the highest level of transcriptional activity. The protein expression in constructed vectors was evaluated by measuring the fluorescence of green fluorescent protein (GFP) and SDS-PAGE. C. glutamicum harboring plasmids showed GFP fluorescence with an order of activity of $P_{ilvC}$ > $P_{ilvC-M1}$ > $P_{sod}$ > $P_{ilvC-M2}$ > $P_{sod-M}$, whereas all plasmids except pCSP30 with $P_{sod}$ displayed fluorescence activities in E. coli. Of them, the strongest level of GFP was observed in E. coli with $P_{sod-M}$, and this seems to be due to the introduction of the conserved SD sequence in the translational initiation region. These results demonstrate that the expression vectors work well in both C. glutamicum and E. coli for the expression of target proteins. In addition, the vector systems harboring various promoters with different strengths, conserved SD sequences, and multiple cloning sites will provide a comfortable method for cloning and gene expression, and consequently contribute to the metabolic engineering of C. glutamicum.

• Journal of the Korean Data and Information Science Society
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• v.23 no.4
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• pp.739-748
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• 2012

In this paper we propose a mixed effects least squares support vector machine (LS-SVM) for the censored data which are observed from different groups. We use weights by which the randomly right censoring is taken into account in the nonlinear regression. The weights are formed with Kaplan-Meier estimates of censoring distribution. In the proposed model a random effects term representing inter-group variation is included. Furthermore generalized cross validation function is proposed for the selection of the optimal values of hyper-parameters. Experimental results are then presented which indicate the performance of the proposed LS-SVM by comparing with a standard LS-SVM for the censored data.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.411-411
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• 2019

지형학적으로 다양한 형상을 가지고 있는 하천유역은 지역적 조건에 따라 뚜렷한 특징을 나타낸다. 이러한 조건은 하천유역의 발달 및 수문학적 특징에 영향을 미친다. 금회 연구는 여러 가지 유역의 특징 중 유역을 대표할 만한 특징을 이용하여 간단하고 유용한 하천 유역 분류 기법을 제시하였다. 하천유역의 여러 특징 중 지류교차각(Tributary Junction Angle)은 유역을 분석하기 위해 많이 사용되었으며 다른 특징들과 함께 유역 구분을 위해 분석되어 왔다. 하지만, 지류교차각만 이용하여 유역 분류를 제시하는 기법은 연구되지 않았다. 하천유역 분류 기법 제시를 위해 수지형 유역, 평행형 유역, 부채형 유역, 직사각형 유역, 격자형 유역 등 5가지의 형태를 중심으로 50개의 하천유역을 사용하였고, 지류교차각의 Beta Distribution 모델을 적용하여 매개변수 추정치 산정 후 유역 분류를 위한 분석을 실시하였다. 매개변수 추정치는 각 유역 형태 구분을 위해 적용되었고, 이후 Support Vector Machines를 이용하여 하천유역 형태를 분류하도록 하였다. 분석을 통한 결과는 일반적인 통계기법과 다른 유역형태 구분 기법을 이용하여 검증하였다. 제안된 기법은 수지형, 평행형, 부채형 유역 형태들에 대하여 정확하게 분류할 수 있으며, 얻어지는 결과는 중요한 수문학적 정보 제공에 사용 될 것으로 판단된다. 금회 연구를 통해 Beta 분포형의 매개변수 추정치는 하천유역 분류 적용에 유용하게 사용 될 수 있음을 확인하였고, 하나의 주요 유역 인자로 유역 구분이 가능함을 제시하였다. 향후 연구로는 하천유역 분류를 통해 수문학적인 동질 유역을 구분하여 수문모델의 수행능력을 향상 시킬 수 있는 수문모델 분석과 개발에 적용 될 수 있을 것이다.

• Journal of the Korean Society of Marine Environment & Safety
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• v.25 no.4
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• pp.498-503
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• 2019

Electric propulsion ships are gaining widespread interest in the marine industry owing to extreme air pollution concerns. Consequently, several studies are actively being conducted for improving the power quality. Various methods have been developed that incorporate passive filters, notch filters, and active filters for reducing the harmonic content in the input current of a conventional diode front end rectifier. Among such filters, the active front end (AFE) rectifier is considered as an excellent technology. In this paper, current control for an AFE rectifier employing space vector PWM (Pulse Width Modulation) is proposed. Conventional current control methods for the AFE rectifier, hysteresis, SPWM (Sinusoidal Pulse Width Modulation), and SVPWM (Space Vector Pulse Width Modulation) were simulated by employing the PSIM software tool for analysis and comparisons. The results corroborate that SVPWM has the simplest structure and provides the best performance.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.16 no.1
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• pp.153-172
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• 2022

In this study, the authors intend to analyze factors contributing to research performance using Backpropagation Neural Network and Support Vector Machine. The analyzing factors contributing to lecturer research performance start from defining the features. The next stage is to collect datasets based on defining features. Then transform the raw dataset into data ready to be processed. After the data is transformed, the next stage is the selection of features. Before the selection of features, the target feature is determined, namely research performance. The selection of features consists of Chi-Square selection (U), and Pearson correlation coefficient (CM). The selection of features produces eight factors contributing to lecturer research performance are Scientific Papers (U: 154.38, CM: 0.79), Number of Citation (U: 95.86, CM: 0.70), Conference (U: 68.67, CM: 0.57), Grade (U: 10.13, CM: 0.29), Grant (U: 35.40, CM: 0.36), IPR (U: 19.81, CM: 0.27), Qualification (U: 2.57, CM: 0.26), and Grant Awardee (U: 2.66, CM: 0.26). To analyze the factors, two data mining classifiers were involved, Backpropagation Neural Networks (BPNN) and Support Vector Machine (SVM). Evaluation of the data mining classifier with an accuracy score for BPNN of 95 percent, and SVM of 92 percent. The essence of this analysis is not to find the highest accuracy score, but rather whether the factors can pass the test phase with the expected results. The findings of this study reveal the factors that have a significant impact on research performance and vice versa.

• Information Systems Review
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• v.19 no.1
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• pp.123-145
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• 2017

This study empirically examines the effects of regulation on online gaming. Going beyond ad hoc heuristic approaches on individual behavior, we investigate the effects of regulation on dynamic changes of games or service providers. In particular, we propose three theoretical perspectives: social influence to investigate the regulation effect, the role of prior experience to determine the difference in the regulation effect size through users' prior experience, and network externalities to discover the difference in the regulation effect size according to the number of users on an online gaming platform. We use the vector autoregression methodology to model patterns of the co-movement of online games and to forecast game usage. We find that online gamers are heterogeneous. Therefore, policy makers should make suitable regulations for each heterogeneous group to effectively avoid generating gaming addicts without interrupting the economic growth of the online gaming industry.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.171-191
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• 2024

Identification of Helicotylenchus species is very challenging due to phenotypic plasticity and existence of cryptic species complexes. Recently, the use of rDNA barcodes has proven to be useful for identification of Helicotylenchus. Molecular markers are a quick diagnostic tool and are crucial for discriminating related species and resolving cryptic species complexes within this speciose genus. However, DNA barcoding is not an error-free approach. The public databases appear to be marred by incorrect sequences, arising from sequencing errors, mislabeling, and misidentifications. Herein, we provide a comprehensive analysis of the newly obtained, and published DNA sequences of Helicotylenchus, revealing the potential faults in the available DNA barcodes. A total of 97 sequences (25 nearly full-length 18S-rRNA, 12 partial 28S-rRNA, 16 partial internal transcribed spacer [ITS]-rRNA, and 44 partial cytochrome c oxidase subunit I [COI] gene sequences) were newly obtained in the present study. Phylogenetic relationships between species are given as inferred from the analyses of 103 sequences of 18S-rRNA, 469 sequences of 28S-rRNA, 183 sequences of ITS-rRNA, and 63 sequences of COI. Remarks on suggested corrections of published accessions in GenBank database are given. Additionally, COI gene sequences of H. dihystera, H. asiaticus and the contentious H. microlobus are provided herein for the first time. Similar to rDNA gene analyses, the COI sequences support the genetic distinctness and validity of H. microlobus. DNA barcodes from type material are needed for resolving the taxonomic status of the unresolved taxonomic groups within the genus.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.29 no.5
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• pp.997-1006
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• 2019

To overcome the difficulties and inefficiencies of the existing power analysis attack, we try to extract the secret key embedded in a cryptographic device using attack model based on MLP(Multi-Layer Perceptron) method. The target of our proposed power analysis attack is the AES-128 encryption module implemented on an 8-bit processor XMEGA128. We use the divide-and-conquer method in bytes to recover the whole 16 bytes secret key. As a result, the MLP-based power analysis attack can extract the secret key with the accuracy of 89.51%. Additionally, this MLP model has the 94.51% accuracy when the pre-processing method on power traces is applied. Compared to the machine leaning-based model SVM(Support Vector Machine), we show that the MLP can be a outstanding method in power analysis attacks due to excellent ability for feature extraction.

• Journal of the Korean Society of Marine Environment & Safety
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• v.27 no.1
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• pp.193-200
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• 2021

In this study, an analysis technique using static condensation is proposed for an efficient local nonlinear static analysis. The static condensation method is a model reduction method based on the degrees of freedom, and the analysis model is divided into a target part and a condensed part to be omitted. In this study, the nonlinear and linear parts were designated to the target and the omitted parts, respectively, and both the stiffness matrix and load vector corresponding to the linear part were condensed into the nonlinear part. After model condensation, the reduced model comprising the stiffness matrix and the load vector for the nonlinear part is constructed, and only this reduced model was updated through the Newton-Raphson iteration for an efficient nonlinear analysis. Finally, the efficiency and reliability of the proposed analysis technique were presented by applying it to various numerical examples.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.102-109
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• 2010

Fructan has been found to accumulate in various tissues during periods when light levels increased carbon fixation where low temperatures reduced growth rates while photosynthesis continued. In this study, we have cloned 1-sucrose:sucrose fructosyl transferase(1-sst) and 1-fructan: fructan fructosyl transferase (1-fft, a key enzyme for the synthesis of fuctan) from Jerusalem Artichoke (Helianthus tuberosus L.). The recombinant vector with 1-sst and 1-fft has been constructed under the control of 35S promoter of KJGV-B2 vector and transgenic plants obtained by Agrobacterium tumefaciens LBA4404. PCR analysis carried out on the putative transgenic plants for amplification of the coding region of specific gene (1-sst, 1-fft), and HPT genes. Transgenic lines carrying of 1-sst and 1-fft were confirmed for integration into the rice genome using Southern blot hybridization and RT-PCR. The transgenic plants in $T_2$ generation were selected and expression pattern analysis revealed that 1-sst and 1-fft were stable. This analysis confirmed the presence of low-molecular-weight fructan in the seedling of the transgenic rices. Therefore, cold tolerance and carbohydrate metabolism will be possible to develop resistant plants using the transgenic rice.