Browse > Article
http://dx.doi.org/10.5010/JPB.2010.37.1.102

Increment of fructan biosynthesis in rice by transformation of 1-sst and 1-fft genes isolated from jerusalem artichoke (Helianthus tuberosus L.)  

Kang, Kwon-Kyoo (Hankyong National University)
Song, Beom-Heon (Chungbuk National University)
Lee, Gyong-A (Chungbuk National University)
Lee, Hye-Jung (Chungbuk National University)
Park, Jin-Ha (Chungbuk National University)
Jung, Yu-Jin (Hankyong National University)
Cho, Yong-Gu (Chungbuk National University)
Publication Information
Journal of Plant Biotechnology / v.37, no.1, 2010 , pp. 102-109 More about this Journal
Abstract
Fructan has been found to accumulate in various tissues during periods when light levels increased carbon fixation where low temperatures reduced growth rates while photosynthesis continued. In this study, we have cloned 1-sucrose:sucrose fructosyl transferase(1-sst) and 1-fructan: fructan fructosyl transferase (1-fft, a key enzyme for the synthesis of fuctan) from Jerusalem Artichoke (Helianthus tuberosus L.). The recombinant vector with 1-sst and 1-fft has been constructed under the control of 35S promoter of KJGV-B2 vector and transgenic plants obtained by Agrobacterium tumefaciens LBA4404. PCR analysis carried out on the putative transgenic plants for amplification of the coding region of specific gene (1-sst, 1-fft), and HPT genes. Transgenic lines carrying of 1-sst and 1-fft were confirmed for integration into the rice genome using Southern blot hybridization and RT-PCR. The transgenic plants in $T_2$ generation were selected and expression pattern analysis revealed that 1-sst and 1-fft were stable. This analysis confirmed the presence of low-molecular-weight fructan in the seedling of the transgenic rices. Therefore, cold tolerance and carbohydrate metabolism will be possible to develop resistant plants using the transgenic rice.
Keywords
1-sst; 1-fft; fructan; carbohydrate metabolism; cold tolerance;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Vijn I, Smeekens S (1999) Fructan: more than a reserve carbohydrate, Plant Physiol. 120:351-360   DOI
2 Wagner W, Keller F, Wiemken A (1983) Fructan metabolism in cereals: induction in leaves and compartmentation in protoplasts and vacuoles, J. Plant Physiol. 112:359-372
3 Wiemken A, Sprenger N, Boller T (1995) Fructanan Extension of Sucrose by Sucrose. In: Pontis HG, Salerno GL, Escheverria EL (eds) Sucrose Metabolism, Biochemistry, Physiology and Molecular Biology, American Plant Physiology Society, Gainesville
4 Thomson T. (1818) In A System of Chemistry, 5th London edition; Abraham Small: Philadelphia
5 Vergauwen R, Van Laere A, Van den Ende W (2003) Properties of fructan:fructan 1-fructosyltransferases from Cichorium intybus L. and Echinops ritro L., two asteracean plants storing greatly different types of inulin, Plant Physiology 133:391-401   DOI
6 Hendry GAF (1987) The ecological significance of fructan in contemporary flora, New phytol. 106:201-216   DOI
7 Hochstrasser U, Luscher M, Virgilio CD, Boller T, Wiemken A (1998) Expression of a functional barley sucrose-fuctan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris, FEBS Letters. 440:356-360   DOI
8 Ingrid MM, Andries JK, Johanna CH, Arjen JT (1998) Cloning of the fructan biosynthesis pathway of Jerusalem artichoke. The plant Journal 15(4):489-500   DOI
9 Livingston III DP, Hincha DK, Heyer AG (2009) Fructan and its relationship to abiotic stress tolerance in plants, Cell. Mol. Life Sci. 66:2007-2023   DOI
10 Lewis DH (1993) Nomenclature and diagrammatic representation of oligomeric fructans - paper for discussion, New Phytologist, 124:583-594   DOI
11 Sambrook J, Russel DW (2001) Molecular Cloning: A Laboratory Manual (third ed.), Cold Spring Harbor Laboratary Press, New York
12 Pollock CJ, Cairns AJ (1991) Fructan metabolism in grasses and cereals. Ann. Rev. Plant Physiol. Plant Mol. Biol. 42:77-101   DOI
13 Roover JD, Vandenbranden K, Laere AV, Eude WVD (1999) Drought induces fructan synthesis and 1-sst (Sucrose:Sucrose 1-Fructosyltransferase) in root and leaves of chicory seedlings (Cichorium intybus L.), Planta 210:808-814   DOI
14 Rose V. (1804) Uber eine eigenthumliche vegetabilische Substanz, Neues Allg. Jahrb. Chem. 3:217-219
15 Stevens CV, Meriggi A, Booten K (2001) Chemical Modification of Inulin, a Valuable Renewable Resource, and Its Industrial Applications. Biomacromolecules 2:1-16   DOI
16 Chirgwin JJ, Przbyla AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease, Biochemistry 18:5294   DOI
17 Edelman J, Jefford TG (1968) The mechanism of fructan metabolism in higher plants as exemplified in Helianthus tuberosus, New Phytol. 67:517-531   DOI
18 Hellwege EM, Raap M, Gritscher D, Willmitzer L, Heyer AG (1998) Differences in chain length distribution of inulin from Cynara scolymus and Helianthus tuberosus are reflected in a transient plant expression system using the respective 1-fft cDNAs, FEBS Letter. 427:25-28   DOI
19 Eude WVD, Michiels A, Wonterghem DV, Vergauwen R, and Laere AV (2000) Cloning, Developmental, and Tissue-Specific Expression of Sucrose:Sucrose 1-Fructosyl Transferase from Taraxacum officinale. Fructan Localizatil in Root, Plant Physiol. 123:71-79   DOI
20 Hellwege Em, Gritscher D, Willmitzer L, Heyer G (1997) Transgenic potato tubers accumulate high levels of 1-kestose and nystose: functional identification of a sucrose sucrose 1-fructosyltransferase of artichoke (Cynara scolymus) blossom discs, The Plant Journal. 12:1057-1065   DOI