• Korean Journal of Plant Taxonomy
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• v.48 no.2
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• pp.143-152
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• 2018

A comparative study of leaf epidermal microstructures in genus Aruncus (two species, five varieties) was carried out using scanning electron microscopy in order to evaluate their significance in terms of taxonomy. All of the leaves of the taxa studied here were amphistomatic with undulate anticlinal walls, and smooth and flat periclinal walls on both surfaces. The size range of the stomata complex is $8.95-21.97{\times}7.50-16.99{\mu}m$: the largest one was found in Aruncus dioicus var. astilboides (average $18.01{\times}13.47{\mu}m$) and the smallest was measured and determined to be A. gombalanus (average $11.11{\times}8.94{\mu}m$). An anomocytic stomata complex was found in all of the studied taxa. The stomatal frequency on average was $27.54/0.05mm^2$; it is highest in A. gombalanus ($60.4/0.05mm^2$) and lowest in A. dioicus var. acuminatus ($11.6/0.05mm^2$). Two types (short stalked capitate glandular trichome and non-glandular trichome) of trichomes are found in the leaves. The non-glandular trichome was divided into three types based on the presence and degree of development of subsidiary cells. Anomocytic stomata of the hypostomatic type and the distribution pattern of capitate glandular trichomes were the major characters in this genus. The stomata size and frequency, the epidermal cell structure, the trichome type and the distribution pattern may have diagnostic importance among the taxa in the genus. Our leaf micromorphological results provide useful information for the taxonomic revision of the genus Aruncus.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.396-418
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• 2018

This study was carried out to investigate the flora and the vegetation of Samusan mountain on Jecheon-si, located in the middle part of the Korean peninsula. The vascular plants which were collected in 9 times from June 2014 to October 2015 were identified as 502 taxa in total, including 102 families, 314 genera, 445 species, 6 subspecies, 49 varieties, 1 form and 1 hybrid. The largest families were as follows; Asteraceae (70 taxa), Poaceae (52 taxa), Rosaceae (30 taxa), Fabaceae (28 taxa), and Cyperaceae (20 taxa). Of them, Korean endemic plants numbered 10 taxa, and vascular plants listed in the red data according to the International Union for Conservation of Nature (IUCN) numbered 14 taxa. The floristic regional indicator plants found in this area were 61 taxa in total. Among them, 5 taxa revealed the floristic grade V, 11 for floristic grade IV, 14 for floristic grade III, 10 for floristic grade II, and 21 for floristic grade I. The alien plants were identified as 43 taxa and the percentage of naturalized index (NI) was 8.6%, and urbanization index (UI) was 13.4%, respectively. Samples of the forest vegetation on the Samusan Mt. were mainly classified as Pinus densiflora, Quercus variabilis, Q. acutissima, Q. mongolica, Zelkova serrata and Robinia pseudoacacia forest. The vertical structures of the forest were stable and the DBH-Class analyses showed that the dominant tree species would be maintained. In the surveyed areas, high plant diversity was shown, and a number of endemic, rare, calcicole plants and phytogeographically important plants were found. Nonetheless, numerous and diverse biological resources native have been consistently disturbed or damaged by human activities without some form of protection. Therefore, it is needed to set up strategies for conservation forest vegetation in this study area.

• Korean Journal of Soil Science and Fertilizer
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• v.28 no.2
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• pp.165-175
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• 1995

To determine the effect of soil moisture stress on growth of barley and grain quality, a pot experiment was carried out for two barley varieties(Olbori and Chogangbori) by using large plastic pot(52cm in diameter and 55cm in depth) filled with sandy loam soil under rain-controlled open green house. By means of measuring soil water potential with micro tensiometer and gypsum block installed at 10cm in soil depth, soil moisture was controlled by sub-irrigation at several irigation points such as -0.05bar, -0.2bar, -0.5bar, -1.0bar, -5.0bar and -10.0bar in soil water potential. The lower soil water potential was controlled, the shorter length of stem and internode became, and the more narrow stem diameter was. Leaf area was significantly decreased when soil water potential was controlled lower than -0.5bar, although chlorophyll content of flag and first leaves was not changed so much. Weight of grain and ear was significantly decreased when soil water potential was lower than -5.0bar and the highest grain yield was obtaind in a plot where soil water potential was controlled at -0.2bar. However, the most efficient water use of Olbori and Chogangbori was obtained at -0.5bar and -1.0bar in water potentials, respectively. Crude protain content, maximum viscosity, consistency and ${\beta}$-glucan content of barley flour increased as soil water potential significantly decreased, especially below -5.0bar, but gelatination temperature decreased as soil water potential decreased.

• Journal of Sericultural and Entomological Science
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• v.22 no.1
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• pp.7-25
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• 1980

Some substances and freezing tolerance in the mulberry (Morus species) branch have been studied on the basis of varietal differences and harvesting times along with harvesting methods in autumn. The results obtained are summarized as follows; 1. The highest freezing tolerance was shown in the varieties of Yongcheon-chou, Jasan, Kang-weon No. 3 and Ichihei, the medium in Roso. Kairyonezumigaeshi, Yanagida and Kokuso No. 28, and the lowest in Ichinose, Mokuso, Kokuso No. 21 and Suweousang No. 3. 2. There was a signifiant negative correlation (r= -0.59＊) between death atop percentage in the field and the temperature required to kill 50% of the mulberry buds (T$_{50}$) with the harvesting times and methods in autumn. Cold hardening occurred in the early through the end of September with the peak at the mid-september. During this period, leaf harvest decreased freezing tolerance with remarkable decrease due to picking all the leaves and leaving several leaves at the base of branch. Greater cold hardening was induced by leaving several leaves after topping. 3. Negative correlations were observed between freezing tolerance and the contents of soluble (r =-0.70＊) and crude (r= -0.70＊) protein. However, positive correlations were shown between freezing tolerance and total carbohydrate contents per crude (r=0.31＊) and per soluble (r=0.71＊) protein . There were also positive correlations between freezing tolerance and total sugar (r=0.67＊) and RNA content (r=0.99＊＊). No relationships of dry matter. fat. total carbohydrate and DNA contents were observed to the freezing tolerance. 4. Such sugars as raffinose. lactose, sucrose, glucose, fructose. arabinose. xylose. ribose (assumed) and rhamnose were detected in winter mulberry branch. Major sugars such as sucrose, glucose, and fructose were supposed to have higher relationship to the freezing tolerance than the other sugars. 5. Late harvesting increased RNA content except in the case of total leaf picking at mid-September. Leaf picking decreased RNA content. Some amount of RNA was, however, maintained by leaving several leaves after topping Leaving upper-middle leaves of a branch showed high RNA content. Leaving young leaves at the top and the overmatured leaves at the base showed low content. A positive correlation (r=0.51＊) was noted between RNA content and freezing tolerance in the different harvesting methods.s.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.6
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• pp.1245-1251
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• 2011

As a result of the growing livestock industry, varieties of organic solid and waste biomass are be generated in swine breeding and slaughtering stages. Anaerobic digestion is a promising alternative for the treatment of livestock waste biomass, as well as for the material recovery and energy production. Objectives of this study were to analyze the biochemical methane potential of swine waste biomasses that were generated from swine pen and slaughterhouse and to investigate the material recovery and methane yield per head. As pig waste biomass, swine slurry, blood, intestine residue, and digestive tract content were collected for investigation from pig farmhouse and slaughterhouse. The $B_{th}$ (Theoretical methane potential) and $B_0$ (Biochemical methane potential) of swine slurry generating in swine breeding stage were 0.525 and $0.360Nm^3\;kg^{-1}-VS_{added}$, the ratio of degradation ($B_0/B_{th}$) was 68.6%. $B_{th}$ of blood, intestine residue, and digestive tract content were 0.539, 0.664, and $0.517Nm^3\;kg^{-1}-VS_{added}$, and $B_0$ were 0.405, 0.213, and $0.240Nm^3\;kg^{-1}-VS_{added}$, respectively. And the ratio of degradation showed 75.1, 32.1, and 46.4% in blood, intestine residue, and digestive tract content. Material yield of swine waste biomass was calculated as TS 73.79, VS 46.75, TN 5.58, $P_2O_5$ 1.94, and $K_2O$ $2.91kg\;head^{-1}$. And methane yield was $16.58Nm^3\;head^{-1}$. In the aspect that slaughterhouse is a large point source of waste biomass, while swine farmhouse is non-point source, the feasibility of an anaerobic digestion using the slaughtering waste biomass need to be assessed in the economical aspect between the waste treatment cost and the profitable effect by methane production.

• Korean Journal of Food Science and Technology
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• v.53 no.5
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• pp.607-612
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• 2021

This study investigated the seed and water absorption characteristics of red bean cultivars in Korea. The length and width of all seeds were 7.74-8.99 mm and 5.33-5.54 mm, respectively. The hilum lengths and widths were 3.21-4.01 mm and 0.58-0.73 mm, respectively. The weight of 100 seeds was 12.14-20.21 g, and the seed coat thickness of red beans ranged from 39.18-50.01 mm. During soaking for 30 h, red beans exhibited a lag phase in the initial soaking time, followed by a rapid absorption phase, and finally contained more than 130% moisture. When water absorption was applied to the sigmoid equation, the curve fitting exhibited a high R², and the kinetics of water absorption were 0.202-0.715 h^-1, which differed among varieties. Pearson's correlation of hilum width (r=0.7858^*) and seed thickness (r= -0.9954^***) exhibited strong correlations with water absorption, suggesting that these are important factors in red bean processing.

• Journal of Mushroom
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• v.19 no.3
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• pp.166-175
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• 2021

In this study, monokaryons of "Heukari" (Pleurotus ostreatus) and "Hosan" (Pleurotus pulmonarius) were separated to remove the cell wall, and a cross-species protoplast fusion was developed through chemical treatment with polyethylene glycol. The protoplast-fused PF160306 and PF160313 strains have a culture period of 10 and 2 days shorter than that of the "Heuktari" and "Hosan" cultivars, respectively. Furthermore, the growth of the strains was faster than that of the existing cultivars. The yield was 135.9 g per bottle, which was approximately 8% higher than that of the commercially available "Hosan" cultivar; however, it was not statistically significant. A growth survey was conducted after treatment at five temperatures (15, 18, 21, 23, and 25℃). The growth of the strains accelerated with the increase in temperature. However, at 21℃, the yellow color of pileus was the brightest. Band pattern, assessed using URP Primer 7, was similar to that of the "Hosan" cultivar. The DPPH radical scavenging capacity and polyphenol content were 62.5% and 43.5 mg/mL, respectively, for "Sunjung" and 65.7% and 49.9 mg/mL, respectively, for PF160313. Furthermore, the antihypertensive activities of the "Sunjung" cultivar and PF160313 were similarly high at 74% and 75%, respectively. In conclusion, cross-species hybridization via the protoplast fusion technique can be used for obtaining primary data for mushroom breeding to develop new varieties. In addition, the protoplast fusion technique might aid in expanding the market for yellow mushrooms.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.320-327
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• 2021

Freezing damage to fruit trees is frequently occurring due to cold in winter and low temperature in spring to abnormal weather caused by global warming. In particular, the freezing injury of deciduous fruit trees is highly dependent on the developmental stages of the flower buds. And the cold resistance is weakened as the growth progresses, so it is most vulnerable period from flowering to petal fall(post-bloom). Therefore, this study was conducted to analyze the cause of the freezing injury caused by severe low temperature to 'Fuji', which has a late flowering period more than 'Hongro' in April 2020. We investigated freezing injury rate in 'Fuji' and 'Hongro' apple trees damaged by natural low temperature at Boeun-gun, Chungbuk province in Korea. In addition, flower buds in the same developmental stage (tight cluster) were treated artificially low temperature to investigate the injury rate for accurate comparative analysis between varieties, and to analyze the soluble sugar and hormone contents in the flower buds. As a result of survey in natural low temperature, 'Fuji' had a higher injury rate than 'Hongro' in both orchards, and in particular, B orchard 'Fuji' had the highest injury rate of 60.5%. Also there were significantly difference in the freezing injury rate between 'Fuji' and 'Hongro' in artificially low temperature treatments. As a result of analyzing the soluble sugar contents in 'Hongro' was higher than 'Fuji'. Also ABA, IAA and SA contents were more increased in the damaged tissue than in the normal flower buds by low temperature treatments. Consequently, it was assumed that the freezing injury was closely related to soluble sugar contents in the flower buds. In particular, the freezing injury rate was negatively correlated with the sorbitol contents.

• Korean Journal of Plant Taxonomy
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• v.51 no.3
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• pp.250-293
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• 2021

Limestone areas are sedimentary rock outcrops consisting of calcium carbonate created several hundreds of millions of years ago by calcium-secreting marine organisms and subsequently lifted above sea level by tectonic movement. Limestone areas support very high levels of endemic species of plants and are recognized as biodiversity areas with much biological information. The purpose of this study is to devise a strategy for the comprehensive conservation of the vegetation of limestone areas through analyses of the floristics and plant species compositions in ten limestone areas on the Korean Peninsula. The results of 153 field surveys from April of 2010 to October of 2016 identified 1,202 taxa in total, representing 1,096 species, 18 subspecies, 84 varieties, 2 forms, and 2 hybrids in 530 genera and 133 families. Among them, 55 taxa were endemic plants to Korea, and 38 taxa were red data plants. The floristic target plants amounted to 102 taxa, specifically 27 taxa of grade V and 75 taxa of grade IV. In all, 121 alien plants were recorded in the investigated area. Calciphilous plants amounted to 102 taxa, specifically 14 taxa of calciphilous indicator plants, 30 taxa of superlative most calciphilous plants, and 58 taxa of comparative more calciphilous plants. A cluster analysis showed a high degree of similarity between sites that are geographically adjacent with similar habitat environments. Limestone areas also supported groups distinct from those in non-limestone areas, demonstrating the specificity of limestone flora. Plant geography approaches therefore appear to be crucial to gain a better understanding of the level of biodiversity in limestone areas, not only at the interspecific but also at the intraspecific level. These results highlight the importance of protecting limestone habitats to preserve not only their interspecific but also the intraspecific diversity, which is highly threatened.

• Korean Journal of Food Science and Technology
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• v.53 no.4
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• pp.375-381
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• 2021

This study investigated the physicochemical properties and chemosensory characteristics of radish samples. Brix results in Matdong-radish showed the highest value (1.5±0.1%), and Mansahyungtong-radish showed the lowest. In terms of salinity, Mansahyungtong-radish had the highest value (1.2±0.1%), and there were no significant differences among samples except Mansahyungtong-radish. In pH analysis, Cheongjunggowon-radish had the highest value at 6.69±0.02. The pH in Mansahyungtong-radish showed the lowest value at 6.10±0.01. In the electronic tongue, sourness was high in the Seoho-radish (8.2), and saltiness was high in the Matdong-radish (8.1). Umami was high in the Seoho-radish (8.3), and sweetness was high in the Matdong-radish (8.1). In the electronic nose, sulfur-containing compounds were high in all the samples. Methanethiol, which represented the odor description of cabbage, garlic, and sulfurous, was abundant in sulfur-containing compounds. Multivariate analysis using physicochemical and chemosensory properties can be used as a database for the food industry.