Major contributions has been made in cellular ultrastructure studies with the use of high voltage electron microscopy (HVEM) and tomography. Applications of HVEM, accompanied by appropriate image processing, have provided great improvements in the analysis of three-dimensional cellular structures. In the present study, structural patterns of the crystalline bodies that are distinguished in mesophyll plastids of CAM-performing Sedum rotundifolium L., have been investigated using HVEM and tomography. Tilting, and diffraction pattern analysis were performed during the investigation. The titlting was performed at ${\pm}60^{\circ}\;with\;2^{\circ}$ increments while examining serial sections ranging from 0.125 to $1{\mu}m$ in thickness. The young plastids exhibited crystalline inclusion bodies that revealed a peculiar structural pattern. They were irregular in shape and also variable in size. Their structural attributes affected the plastid morphology. The body consisted of a large number of tubular elements, often reaching up to several thousand in number. The tubular elements typically aggregated to form a fluster The elements demonstrated either a parallel or lattice arrangement depending on the sectioning angle. The distance between the elements was approximately 20nm as demonstrated by the diffraction analysis. HVEM examination of the serial sections revealed an occasional fusion or branching of elements within the inclusion bodies. Finally, a three-dimensional reconstruction of the plastid crystalline bodies has been attempted using two different image processing methods.
Hydrogeological modelling was performed to evaluate groundwater flow system in Igsan Area. The study area extends over $790km^2$. The geology consists of Jurassic Daebo granite and gneissose granite and Precambrian metamorphic rocks. The capability of pumping yield is the highest in gneissose granite region among them due to comparatively thick weathered zone with thickness ranging from 10m to 25m. The Colorado State University Finite Difference Model was used for the model simulation. The model was divided into 28 rows and 31 columns with variable grid spacing. The model was calibrated under steady-state and unsteady-state conditions. In the steady-state simulation, the model results were compared with measured water table contours in September 1985 with determining hydraulic conductivities and net recharge rates during rainy season. Unsteady state simulation was done to know the aquifer response due to groundwater abstraction. The non- steady state calibration was conducted to determine the distribution and magnitudes of specific yields and discharge/recharge rates during dry season as matching water level altitudes in May 1986. The calibrated model was used to simulate water level vaiation caused by groundwater withdrawal and natural recharge from 1 October, 1985 until 30 September, 1995. The calibrated model can be used to groundwater development schemes on regional groundwater levels, but it cannot be used to simulate local groundwater level change at a specific site.
The skin on the dorsum of the foot is a source of the reliable thin and sensory cutaneous free tissue transplantation with or without tendon, bone and joint. A composite flap with attached vascularized tendon grafts for the combined loss of skin and tendon on the dorsum of the hand and foot offers an immediate one stage solution to this problem. The flap provides a very durable innervated tissue cover for the heel of the foot and the dorsum of the hand and an osteocutaneous transfer combined with the second metatarsal. The major dorsalis pedis artery is constant in size, but the first dorsal metatarsal artery is variable in size and location. The dorsal surface of the foot receives sensory innervation through the superficial peroneal nerve and the first web through the deep peroneal nerve. Authors had performed 5 dorsalis pedis free flap transplantation in the foot and hand at Department of Orthopedic Surgery, Chonbuk National University Hospital from August 1993 through August 1997 and followed up for the period of between 19 and 67 months until March 1999. The results were as follows 1. 5 cases dorsalis pedis free flap transfer to the foot(4 cases) and the hand(1 case) were performed and the recipient was foot dorsum and heel 2 cases each and hand dorsum 1 case. 2 All of 5 cases(100%) were survived from free flap transfer and recipient artery was dorsalis pedis artery(2 cases), anterior tibial artery(1 case), posterior tibial artery(1 case) and ulnar artery(1 case) and recipient veins were 2 in number except in the hand. 3. Long term follow up of the exterior and maceration was good and sensory recovery was poor 4. Donor site was covered with full thickness skin graft obtained from one or both inguinal areas at postoperative 3rd week and skin graft was taken good and no morbidity was showed.
Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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2001.06a
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pp.1247-1247
/
2001
Constituents of animal biofluids such as milk, blood and urine contain information specifically related to metabolic and health status of the ruminant animals. Some changes in composition of biofluids can be attributed to disease response of the animals. Mastitis is a major problem for the global dairy industry and causes substantial economic losses from decreasing milk production and reducing milk quality. The purpose of this study was to investigate potential of NIRS combined with multivariate analysis for cow's mastitis diagnosis based on NIR spectra of milk, blood and urine. A total of 112 bulk milk, urine and blood samples from 4 Holstein cows were analyzed. The milk samples were collected from morning milking. The urine samples were collected before morning milking and stored at -35$^{\circ}C$ until spectral analysis. The blood samples were collected before morning milking using a catheter inserted into the carotid vein. Heparin was added to blood samples to prevent coagulation. All milk samples were analyzed for somatic cell count (SCC). The SCC content in milk was used as indicator of mastitis and as quantitative parameter for respective urine and blood samples collected at same time. NIR spectra of blood and milk samples were obtained by InfraAlyzer 500 spectrophotometer, using a transflectance mode. NIR spectra of urine samples were obtained by NIR System 6500 spectrophotometer, using 1 mm sample thickness. All samples were divided into calibration set and test set. Class variable was assigned for each sample as follow: healthy (class 1) and mastitic (class 2), based on milk SCC content. SIMCA was implemented to create models of the respective classes based on NIR spectra of milk, blood or urine. For the calibration set of samples, SIMCA models (model for samples from healthy cows and model for samples from mastitic cows), correctly classified from 97.33 to 98.67% of milk samples, from 97.33 to 98.61% of urine samples and from 96.00 to 94.67% of blood samples. From samples in the test set, the percent of correctly classified samples varied from 70.27 to 89.19, depending mainly on spectral data pretreatment. The best results for all data sets were obtained when first derivative spectral data pretreatment was used. The incorrect classified samples were 5 from milk samples,5 and 4 from urine and blood samples, respectively. The analysis of changes in the loading of first PC factor for group of samples from healthy cows and group of samples from mastitic cows showed, that separation between classes was indirect and based on influence of mastitis on the milk, blood and urine components. Results from the present investigation showed that the changes that occur when a cow gets mastitis influence her milk, urine and blood spectra in a specific way. SIMCA allowed extraction of available spectral information from the milk, urine and blood spectra connected with mastitis. The obtained results could be used for development of a new method for mastitis detection.
Daejeonsa basalt in the Mt. Juwang area is composed of 12 lava flows alternate with 9 peperites, and each lava and peperite has variable thickness. Globular peperites yielded in Daejeonsa basalt are mixed basalt clasts with reddish shale. Based on field description, when lava flows over unconsolidated wet shale or injectes into unconsolidated wet shale, peperites were formed at the contacts between lava and shale. Daejeonsa basalt are massive lava flows with rare vesicules: some vesicules are found in upper part of a flow unit. The basalt has mainly pseudomorphs of olivine as phenocryst, and also plagioclase and clinopyroxene phenocrysts in rocks with higher Mg-number. Matrix is mainly subophitic texture, sometimes showing ophitic and intergranular textures due to different cooling rate. Clinopyroxene is augite(Wo$_{41.6}$En$_{45.1}$Fs$_{13.3}$), and plagioclase is mostly labradorite(An$_{55.0}{\sim}_{67.7}$), but some is andesine(An$_{44.3}$) and bytownite(An$_{74.5}$). Oxide minerals are composed of titanomagnetite and ilmenite.
This paper proposes a method that extracts characteristics of cracks such as length, thickness and direction from a concrete slab surface image with image processing techniques. These techniques extract the cracks from the concrete surface image in variable conditions including bad image conditions) using the ART2-based RBF network to recognize the dominant directions -45 degree, 45 degree, horizontal and vertical) of the extracted cracks from the automatically calculated specifications like the lengths, directions and widths of the cracks. Our proposed extraction algorithms and analysis of the concrete cracks used a Robert operation to emphasize the cracks, and a Multiple operation to increase the difference in brightness between the cracks and background. After these treatments, the cracks can be extracted from the image by using an iterated binarization technique. Noise reduction techniques are used three separate times on this binarized image, and the specifications of the cracks are extracted form this noiseless image. The dominant directions can be recognized by using the ART2-based RBF network. In this method, the ART2 is used between the input layer and the middle layer to learn, and the Delta learning method is used between the middle layer and the output layer. The experiments using real concrete images showed that the cracks were effectively extracted, and the Proposed ART2-based RBF network effectively recognized the directions of the extracted cracks.
For the construction of 4.8km long Multi-Purpose Jamuna Bridge in Bangladesh, 2 or 3 large diameter open-ended steel pipe piles were used for the foundation of piers. A total of 123 piles were driven for 50 piers and 2 test piles from the river bed through the normally-consolidated upper sand layer and rested n top of gravel layer. Two types of piles, having 3.15 or 2.50m diameter and variable wall thickness in the range of 40 to 60mm, were driven to the depths of 69 to 74m with the rake of 6:1 by connecting 2 or 3 pieces of short piles. Dynamic pile tests were performed on 24 selected piles during pile driving and soil plug length inside the pile was also measured after driving of each short section.These piles were plugged with soil to, though slightly affected by pile diameters, about 75% of total length of pile driven. Active plug at the tip of pile contributed substantial amount of inner skin friction to the total capacity. Piles soon after driving showed a skin-friction dominant pile behaviour, tat is, 90% of total capacity being developed by skin resistance. Quakes values and Smith damping factors were almost constant regardless of pile diameters. This result reflects the influence of uniform soil condition at the site.
The main purpose of this study is to develop a sprayed FRP repair and strengthening method, which is a new technique for strengthening the existing concrete structures by mixing one of the carbon or glass chopped fibers and one of the epoxy or vinyl ester resins with high-speed compressed air in open air and randomly spraying the mixture onto the concrete surface. At present, the sprayed FRP repair and strengthening method using the epoxy resin has not been fully discussed. In order to investigate the material property of the sprayed FRP, this study carried out tensile tests of the material specimens, which were changed with the combinations of various variables including the length of chopped fiber and the mixture ratio of chopped fiber and resin. These variables were set to have the equal material strength, compared with that of one layer of the FRP sheet. As a result, the optimal length of glass and carbon chopped fibers was fumed out to be 38 mm, and the optimal mixture ratio between chopped fiber and resin was also turned out to be 1 : 2 from each variable. And also, the thickness of the sprayed FRP to have the equal strength to one layer of the FRP sheet was finally calculated. In is study, a series of experiments were carried out to evaluate the strengthening effects of flexural beams, shear beams and damaged beams strengthened with the sprayed FRP method, respectively. The results revealed that the strengthening effects of the flexural and shear specimens were reasonably similar to those of the FRP sheet, and the developed Sprayed FRP technique is able to be used as a strengthening scheme of existing RC building.
Carbon fiber reinforced plastic (CFRP), one of lightweight materials, is the fiber structure using carbon fiber. It is the composite material that has the characteristics of carbon and plastic. As for the fiber structure, it has the great strength due to fiber direction. CFRP for woven type is used mostly as such a CFRP with lightweight. Woven type is more stable when compared with unidirectional type. On the other hand, woven type is highly priced. Therefore, this study aims to analyze the fiber structure of unidirectional CFRP. In this study, as the stacking angle [0/X/-X/0], X is the variable. This is unidirectional CFRP in which the angle phase of X has been reversed and stacked. By using such a unidirectional CFRP, the analysis model which had a crack at the center as the form of panel with the thickness of 2 mm was used. On analysis, the load is applied on the upper and lower parts being connected with a pin. The damage in the area near center crack was investigated. As for the analysis model, 3D surface model was designed by using CATIA. For CFRP stacking, the stacking direction was determined by using ACP in ANSYS program and the analysis model with two stacks was made. Afterwards, the structural analysis was carried out.
Park, Jin Soo;Lee, Hyo Ryong;Lee, Beom Ho;Park, Joon Sik
Korean Journal of Materials Research
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v.25
no.5
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pp.226-232
/
2015
High temperature plasma coating technology has been applied to recover damaged aluminum dies from wear by spraying pure aluminum and alumina powder. However, the coated mixed powder layer composed of aluminum and alumina often undergoes a detachment from the substrate, making the coated substrate die unable to maintain its expected life span. In this study, in order to increase the bonding strength between the substrate and the coating layer, a pure aluminum layer was applied as an intermediate bond layer. In order to prepare the specimen with variable bond coating conditions, the bond coat layers with a various gun speed from 10 cm/sec to 30 cm/sec were prepared with coating cycle variations ranging from three to nine cycles. The specimen with a bond coat layer coated with a gun speed of 20 cm/sec and three coating cycles exhibited ~13MPa of adhesion strength, while the specimen without a bond coat layer showed ~6 MPa of adhesion strength. The adhesion strength with a variation of bond coat layer thickness is discussed in terms of coating parameters.
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