• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1271-1279
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• 2009

To develop low endotoxic and multi-immunogenic outer membrane vesicles (OMVs), a deletion mutant of the msbB gene in Salmonella enterica serovar Typhimurium (S. Typhimurium) was used as a source of low endotoxic OMV, and an expression vector of the canine parvovirus (CPV) VP2 epitope fused to the bacterial OmpA protein was constructed and transformed into the Salmonella ${\Delta}msbB$ mutant. In a lethality test, BALB/c mice injected intraperitoneally with the Salmonella ${\Delta}msbB$ mutant survived for 7 days, whereas mice injected intraperitoneally with the wild type survived for 3 days. Moreover, all mice inoculated orally with the ${\Delta}msbB$ mutant survived for 30 days, but 80% of mice inoculated orally with the wild type survived. The OmpA::CPV VP2 epitope fusion protein was expressed successfully and associated with the outer membrane and OMV fractions from the mutant S. Typhimurium transformed with the fusion protein-expressing vector. In immunogenicity tests, sera obtained from the mice immunized with either the Salmonella msbB mutant or its OMVs containing the OmpA::CPV VP2 epitope showed bactericidal activities against wild-type S. Typhimurium and contained specific antibodies to the CPV VP2 epitope. In the hemagglutination inhibition (HI) assay as a measurement of CPV-neutralizing activity in the immune sera, there was an 8-fold increase of HI titer in the OMV-immunized group compared with the control. These results suggested that the CPV-neutralizing antibody response was raised by immunization with OMV containing the OmpA::CPV VP2 epitope, as well as the protective immune response against S. Typhimurium in BALB/c mice.

• Journal of the Korean Society of Radiology
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• 제8권6호
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• pp.333-338
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• 2014

The fact is that in addition to an increase in social needs that must be managed systematically unnecessary exposure in the field of medical Recent important that the shield has emerged. However, products that are now in practical use, are not subdivided as compared to various medical radiology. Therefore, in the present study, we tried to present with the help of Monte Carlo simulation the structure of the shielding material that has been optimized. Simulated estimation result, the energy of the mammography for (30 kVp) spectrum, check the shielding rate of 90% or more $30{\mu}mPb$, at 2 mmAl case of shielding material of a single, at design time of 1 mmAl and 0.03 mmPb a double shield structure it is determined that more efficient. Also, check the blocking rate of 90% or more $340{\mu}mPb$, at 30 mmAl energy captured general in (80 kVp) spectra, it is considered that a double shield structure, design 1 mmAl and 0.3 mmPb is useful. These results, be used as basic material for the development of commercialization customized products for dose reduction is expected.

• Journal of the Korean Society of Radiology
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• 제13권7호
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• pp.933-939
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• 2019

The purpose of this study is to provide basic clinical data by evaluating images, measuring absorbed dose and effective dose by using high resolution CT and low dose CT by using anthropomorphic chest phantom and glass dosimeter. Tissue dose was measured by inserting a glass dosimeter into the anthropomorphic chest phantom. A 64-slice CT system (SOMATOM Sensation 64, Siemens AG, Forchheim, Germany) and CARE Dose 4D were used, and the parameters of the high resolution CT were 120 kVp, Eff. Scan parameters of mAs 104, scan time 7.93 s, slice 1.0 mm (Acq. 64 × 0.6 mm), convolution kernel (B60f sharp) were used, and low dose CT was 120 kVp, Eff. mAs 15, scan time 7.41 s, slice 3.0 mm (Acq. 64 × 0.6 mm), scan of convolution kernel B50f medium sharp. CTDIvol was measured at 8.01 mGy for high resolution CT and 1.18 mGy for low dose CT. Low dose CT scans showed 85.49% less absorbed dose than high resolution CT scans.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제35권1호
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• pp.26-30
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• 2009

Background and Objectives: To determine the anatomic position of the inferior alveolar nerve (IAN) canal in patients with mandibular prognathism using the cone-beam CT (CBCT). Materials and Methods: Fifty rami from 25 patients were evaluated. The images were taken by i-CAT and reconstructed 3-dimensionally using the Simplant 11 program. The linear distances between the IAN canal to the buccal cortex (a, $a^{\dag}$ and $a^{\ddag}$), from the IAN canal to the alveolar crest (b and $b^{\dag}$) and the anterior margin ($b^{\ddag}$) and finally the buccal cortical thickness (c, $c^{\dag}$ and $c^{\ddag}$) were measured at three reference planes (VP, OP and HP). Results: On the left side, the average distance of a, b and c were 7.12, 15.96 and 3.60 mm on the VP plane, respectively. On the OP, the distance of $a^{\dag}$, $b^{\dag}$ and $c^{\dag}$ was 6.11, 8.83 and 2.63 mm. For the HP, the distance of $a^{\ddag}$, $b^{\ddag}$ and $c^{\ddag}$ was 4.84, 10.11 and 2.30 mm. On the right side, the distance of a, b and c, on the VP, was 7.10, 16.13 and 3.42 mm, respectively. On the OP, the distance of $a^{\dag}$, $b^{\dag}$ and $c^{\dag}$ was 4.77, 8.75 and 2.68 mm. On the HP, the distance of $a^{\dag}$, $b^{\dag}$ and $c^{\ddag}$ was 4.55, 9.84 and 2.38 mm. Regarding the difference between genders, the distance in male's was longer than female's on the VP (p=0.019), and was thicker in males than females on the HP (p=0.002). Conclusion: The CBCT data provided accurate information about the location and course of the IAN.

• Journal of radiological science and technology
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• 제40권3호
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• pp.371-376
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• 2017

Since in case of children, they are sensitive to the radiation compared to the adult and the potential exposure damage lasts longer, the exposure dose should be managed better than for the adult. Therefore, this study was conducted to observe the change in the chest x-ray image by the use of grid, which eliminates the scattering rays but increases the exposure dose during the child chest x-ray examination. As a research method, SNR, CNR and V. Vuichi were measured at 100 cm and 180 cm with the grid varying the kVp to 70, 90 and 110. In addition, SNR, CNR and V. Vuichi were measured fixing 100 cm and 180cm without grid and varying the dose to 6, 8 and 10 mAs. In the results of measuring them by fixing kVp, SNR, VNR and V. Vuichi were represented high when FID is 100cm. And in the results of meaduring them varying mAs, SNR, VNR and V. Vuichi were represented high when FID is 100cm. Currently in our country, the chest x-ray examination is performed at 180 cm. However, as the image is measured high when FID is 100 cm, in case of child, FID is deemed to be 100 cm.

• Journal of Life Science
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• 제23권2호
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• pp.175-181
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• 2013

In Korea, most hepatitis A virus is the IA genotype, but reports of other genotypes have increased recently. Therefore, the purpose of this study is to conduct a genotypic analysis of acute hepatitis A virus. From April 2010 to April 2011, clinical specimens from 20 patients hospitalized with acute hepatitis A and 36 sera positive for anti-HAV IgM were obtained, and the genotype of the VP1/P2A region was analyzed. RNA sequences of the VP1/P2A junction region were amplified using RT-PCR, and the sequences were compared. From 50 sequences amplified, 4 sequences (8%) belonged to genotype IA. The remaining 46 (92%) belonged to genotype IIIA. The results indicate that the genotype of the hepatitis A virus has changed from IA to IIIA in Korea.

• Journal of Radiation Protection and Research
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• 제30권1호
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• pp.9-15
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• 2005

This study was conducted to investigate the patient exposure dose during a CT examination and the present position in utilization of the CT equipments. To this end Questionnaire were sent out to 278 medical facilities registered at Korea Hospital Association and replies of 161 medical facilities were analyzed. The results were as follows ; 1) The distribution of CT examination was. Brain 40.7%, Abdomen 27.8%, Chest 15.7%, Spine 4.8%, Pelvis 4.1%, PNS 3.2%, Facial bone 2.5% and Extremity 1.1% respectively. 2) The statistics for dose index according to each part of examination were Brain 38.0mGy, Abdomen 12.0mGy for adults : Brain 13.6mGy, Abdomen 6.2mGy for infant. Our surveyed dose index appeared lower than the IAEA recommends. 3) Most medical facilities have selected the parameters for radiographic exposure in the range of $100{\sim}120kVp,\;100{\sim}250mA\;and\;1{\sim}2$ seconds.

• Journal of Microbiology and Biotechnology
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• 제27권12호
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• pp.2221-2227
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• 2017

Echovirus serotype 30 (ECHO30) has been responsible for several recent worldwide outbreaks of viral meningitis. In Zhejiang Province, China, ECHO30 has been one of the main causes of viral meningitis for years. This study, using phylogenetic analysis of the VP1 gene, was performed to investigate the general molecular epidemiology and genetic patterns of ECHO30 circulating in Zhejiang Province between the years 2002 and 2015. The nucleotide sequences of ECHO30 VP1 showed that they were 64.8% identical with the prototype strain, Bastianni, while the amino acids were 84.9% identical. Phylogenetic analyses showed that ECHO30 in the Zhejiang area has diverged into two genotypes. Genotype I consists of strains isolated since 2002, whereas genotype II includes strains that were mainly isolated during the 2002 to 2004 outbreak. ECHO30 has been endemically circulating in both humans and the environment for a long period of time. Additionally, we evaluated the significance of recombination presented during the years 2005 to 2007 to demonstrate that recombination plays an important role in the prevalence of ECHO30 in the Zhejiang area.

• Journal of Environmental Health Sciences
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• 제39권2호
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• pp.178-186
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• 2013

Objective: Human enteroviruses (HEVs) are a common causative agent of gastrointestinal or respiratory infections. In this study, to examine the genotypic diversity and characteristics of HEVs associated with patients in Seoul, we collected and analyzed stool and throat swab samples taken from patients with acute gastroenteritis or a common cold from 2011 to 2012. We researched the difference in genetic characteristics of HEVs from gastroenteritis and respiratory patients. Methods: For genetic analysis, we amplified the 5'-noncoding region and partial VP1 region of HEVs by RT-PCR. The genotypes of HEVs were further identified based on nucleotide sequences of the VP1 region. Results: The majority of the HEV infections in Seoul occurred from June to August. The molecular characteristic assay showed that although the majority of HEVs can be propagated by a fecal-oral route, Coxsackievirus A2 (n=13, 19.4%), A4 (n=8, 11.9%), and A5 (n=4, 6.0%) can be preferentially transmitted by a respiratory route. Conclusions: This Enterovirus surveillance system plays an important role in preparing for a severe outbreak. The genotypic characteristics of HEV may provide potentially useful data needed for epidemiological studies.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• 제20권1호
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• pp.113-124
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• 1990

For the study of the influence of kilovoltage and exposure time on radiographic density and contrast, we measured radiographic density of aluminum step wedge which composed of contiguous 8 steps wedges of 2-16㎜ thickness with densitometer. Aluminum step wedge was radiographed on Kodak ultraspeed DF-58 and Ektaspeed EP-21 film with range of 60-90 kVp and 5-60 impulse and subject contrast of aluminum step wedge with constant radiographic density and image contrast percentage without radiographic density was evaluated. Then we evaluated the film quality of teeth and their surrounding structure according to the change of kVp and exposure time by score rating method. The obtained results were as follows: 1. Radiographic density was related to the change of kilovoltage, especially in increased exposure time. 2. With constant radiographic density, subject contrast of thin aluminum step wedges was greater in low kilovolt age than high kilovoltage, but kilovolt age had not great influence on subject contrast of thick aluminum step wedge. On the other hand, radiographic density difference between 2mm and 16mm aluminum step wedge was decreased according to in- creasing kilovoltage. 3. Without constant radiographic density, image contrast percentage was decreased with increasing kilovoltage, but was not related with the change of exposure time. 4. Radiographic contrast of teeth and their surrounding structure which was taken with the range of 60-90 kVp and 6-30 impulse had not great influence on film quality.