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http://dx.doi.org/10.5352/JLS.2013.23.2.175

Characterization of Acute Hepatitis Virus A Genotype in Korea  

Kim, Mi Hyun (Department of Clinical Pathology, Hankook Hospital)
Choi, Hayana (Department of Biology, College of Bio-Industry Science, Sunchon National University)
Pak, Kun Sik (Department of Biology, College of Bio-Industry Science, Sunchon National University)
Seong, Chi Nam (Department of Biology, College of Bio-Industry Science, Sunchon National University)
Cho, Hyun Wook (Department of Biology, College of Bio-Industry Science, Sunchon National University)
Publication Information
Journal of Life Science / v.23, no.2, 2013 , pp. 175-181 More about this Journal
Abstract
In Korea, most hepatitis A virus is the IA genotype, but reports of other genotypes have increased recently. Therefore, the purpose of this study is to conduct a genotypic analysis of acute hepatitis A virus. From April 2010 to April 2011, clinical specimens from 20 patients hospitalized with acute hepatitis A and 36 sera positive for anti-HAV IgM were obtained, and the genotype of the VP1/P2A region was analyzed. RNA sequences of the VP1/P2A junction region were amplified using RT-PCR, and the sequences were compared. From 50 sequences amplified, 4 sequences (8%) belonged to genotype IA. The remaining 46 (92%) belonged to genotype IIIA. The results indicate that the genotype of the hepatitis A virus has changed from IA to IIIA in Korea.
Keywords
Genotype; hepatitis A virus; VP1/P2A region;
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1 Brundage, S. C. and Fitzpatrick, A. N. 2006. Hepatitis A. Am Fam Physician 73, 2162-2168.
2 Chun, J. 1995. Computer-assisted classification and identification of actinomycetes. Ph. D. Thesis, University of Newcastle, Newcastle, United Kingdom.
3 Clemens, R., Safary, A., Hepburn, A., Roche, C., Stanbury, W. J. and André, F. E. 1995. Clinical experience with an inactivated hepatitis A vaccine. J Infect Dis 171(Suppl 1), S44-S49.   DOI   ScienceOn
4 Endo, K., Inoue, J., Takahashi, M., Mitsui, T., Masuko, K. and Akahane, Y. 2007. Analysis of the full-length genome of a subgenotype IIIB hepatitis A virus isolate: primers for broadly reactive PCR and genotypic analysis. J Med Virol 79, 8-17.   DOI   ScienceOn
5 Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783-791.   DOI   ScienceOn
6 Fujiwara, K., Yokosuka, O., Fukai, K., Imazeki, F., Saisho, H. and Omata, M. 2001. Analysis of full-length hepatitis A virus genome in sera from patients with fulminant and self-limited acute type A hepatitis. J Hepatol 35, 112-119.   DOI   ScienceOn
7 Jansen, R. W., Siegl, G. and Lemon, S. M. 1990. Molecular epidemiology of human hepatitis A virus defined by an antigen- capture/polymerase chain reaction method. Proc Natl Acad Sci USA 87, 2867-2871.   DOI   ScienceOn
8 Jeong, S. H. 2008. Current status and vaccine indication for hepatitis A virus infection in Korea. Korean J Gastroenterol 51, 331-337.   과학기술학회마을
9 Jukes, T. H. and Cantor, C. R. 1969. Evolution of protein molecules. In mammalian protein metabolism. pp. 21-132, Academic Press, New York.
10 Jung, Y. K. and Kim, J. H. 2009. Epidemiology and clinical features of acute hepatitis A: from the domestic perspective. Korean J Hepatol 15, 438-445.   과학기술학회마을   DOI   ScienceOn
11 Kim, J. S. and Kim, S. H. 2001. Molecular epidemiology of an outbreak of hepatitis A in Korea. Korean J Clin Pathol 21, 114-118.
12 Kim, S. H., Yoon, H. E., Kim, Y. K., Kim, J. Y., Choi, B. S. Choi, Y. J., Kim, Y. O., Kim, Y. S., Bang, B. K. and Yang, C. W. 2008. Acute hepatitis A associated acute renal failure in adults. Nephron Clin Pract 109, 127-132.   DOI   ScienceOn
13 Lee, D., Cho, Y. A., Park, Y., Hwang, J. H., Kim, J. W. Kim, N. Y., Lee, D. H., Lee, W. and Jeong, S. H. 2008. Hepatitis A in Korea epidemiological shift and call for vaccine strategy. Intervirology 51, 70-74.
14 Lee, K. O., Jeong, S. J., Seong, H. S., Kim, K. T., Hwang, Y. S. Kim, G. Y. and Lee, S. W. 2009. Genetic analysis of hepatitis A virus isolated from Korea. J Bacteriol Virol 39, 165-171.   과학기술학회마을   DOI   ScienceOn
15 Lee, K. Y., Song, K. H. and Kang, J. H. 1998. Seroepidemiology of hepatitis A in Taejon, Korea 1996. J Korean Pediatr Soc 41, 53-61.
16 Lee, Y. W., Yang, H. W., Lee, J. A., Yun, K. H., Yang, S. E., Lee, M. J., Park, S. Y., Kim, S. H., Lee, H. I., Lee, Y. J., Jung, S. H., Kim, A. N., Cha, S. W. and Kim, S. Y. 2011. Clinical differences according to genotype in acute viral hepatitis A in Daejeon, Korea. Korean J Med 80, 553-561.
17 Lemon, S. M. 1997. Type A viral hepatitis: epidemiology, diagnosis, and prevention. Clin Chem 43, 1494-1499.
18 Nainan, O. V., Xia, G., Vaughan, G. and Margolis, H. S. 2006. Diagnosis of hepatitis a virus infection: a molecular approach. Clin Microbiol Rev 19, 63-79.   DOI   ScienceOn
19 Robertson, B. H., Jansen, R. W., Khanna, B., Totsuka, A., Nainan, O. V., Siegl, G., Widell, A., Margolis, H. S., Isomura, S., Ito, K., Ishizu, T., Moritsugu, Y. and Lemon, S. M. 1992. Genetic relatedness of hepatitis A virus strains recovered from different geographical regions. J Gen Virol 73, 1365-1377.   DOI   ScienceOn
20 Park, J. Y., Lee, J. B., Jeong, S. Y., Lee, S. H., Lee, M. A. and Choi, H. J. 2007. Molecular characterization of an acute hepatitis A outbreak among healthcare workers at a Korean hospital. J Hosp Infect 67, 175-181.   DOI   ScienceOn
21 Roh, H. O., Sohn, Y. M., Park, M. S., Choi, B. and Bang, K. N. 1997. A seroepidemiologic study of hepatitis A virus in the healthy children and adolescent in Kyonggi-do province. Korean J Pediatr Infect Dis 4, 232-239.
22 Rueckert, R. R. and Wimmer, E. 1984. Systemic nomenclature of picornavirus proteins. J Virol 50, 957-959.
23 Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406-425.
24 Song, M. H., Lim, Y. S., Song, T. J., Choi, J. M., Kim, J. I., Jun, J. B., Kim, M. Y., Pyun, D. K., Lee, H. C., Jung, Y. H., Lee, Y. S. and Suh, D. J. 2005. The etiology of acute viral hepatitis for the last 3 years. Korean J Med 68, 256-260.   과학기술학회마을
25 Stene-Johansen, K., Jonassen, T. O. and Skaug, K. 2005. Characterization and genetic variability of Hepatitis A virus genotype IIIA. J Gen Virol 86, 2739-2745.   DOI   ScienceOn
26 Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876-4882.   DOI   ScienceOn
27 Yoon, Y. K., Sim, H. S., Kim, J. Y., Park, D. W., Sohn, J. W., Chun, B. C. and Kim, M. J. 2010. Clinical characterization of hepatitis A infection complicated with acute kidney injury and sequence analysis of the VP1 region. Korean J Clin Microbiol 13, 7-13.   DOI   ScienceOn
28 Victor, J. C., Monto, A. S., Surdina, T. Y., Suleimenova, S. Z., Vaughan, G., Nainan, O. V., Favorov, M. O., Margolis, H. S. and Bell, B. P. 2007. Hepatitis A vaccine versus immune globulin for postexposure prophylaxis. N Engl J Med 357, 1685-1694.   DOI   ScienceOn
29 Weitz, M. and Siegl, G. 1985. Variation among hepatitis A virus strains. I. Genomic variation detected by T1 oligonucleotide mapping. Virus Res 4, 53-67.   DOI   ScienceOn
30 Yoon, Y. K., Chun, B. C., Lee, H. K., Seo, Y. S., Shin, J. H. and Hong, Y. S. 2009. Epidemiological and genetic analysis of a sustained community-wide outbreak of hepatitis A in the Republic of Korea, 2008: A hospital-based case-control study. J Clin Virol 46, 184-188.   DOI   ScienceOn