• 제목/요약/키워드: VP4

검색결과 486건 처리시간 0.027초

Molecular characterization of avian rotavirus isolated in Korea

  • Wang, Jun-Hui;Koo, Bon-Sang;Mo, In-Pil;Kang, Shien-Young
    • 한국동물위생학회지
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    • 제36권1호
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    • pp.23-30
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    • 2013
  • An avian rotavirus (AvRV-2) was isolated from feces of broilers suffering from acute gastroenteritis in 2011. It was the first avian rotavirus isolated in Korea. To investigate the molecular characteristics of AvRV-2, the VP4, VP6, VP7 and NSP4 gene nucleotide sequences were determined and compared with those of rotavirus strains available in the GenBank database. The phylogenetic tree of VP7 gene showed that AvRV-2 had a high degree of nucleotide sequence homology (93.4% to 94.7%) with those of rotaviruses belonging to genotype G19 cluster. The phylogenetic tree of the VP4 gene revealed a high degree of nucleotide sequence homology (95.8% to 95.9%) with genotype P[30] rotaviruses isolated from chickens. The VP6 and NSP4 gene nucleotide sequences showed the highest identities with those of avian strains with 95.3% to 96.4% and 90.3% to 92.2%, respectively. Genetic characterization of the VP4, VP6, VP7 and NSP4 showed that AvRV-2 strain was most closely related to chicken rotavirus strains from Germany and Japan. Comparative nucleotide sequences and phylogenetic analysis indicated that avian rotavirus isolated from broilers belonged to genotype G19P[30] and it was the first report on avian rotavirus infection in Korea.

HEVC/VP9 4×4 Transform 통합 블록 설계 (Design of Unified HEVC/VP9 4×4 Transform Block)

  • 정슬기;이성수
    • 전기전자학회논문지
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    • 제19권3호
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    • pp.392-399
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    • 2015
  • 본 논문에서는 HEVC와 VP9 코덱의 계수 변환 (Transform)을 수행하는 통합형 아키텍쳐를 제안하여 하드웨어 크기를 줄이고자 한다. 제안하는 아키텍쳐는 HEVC $4{\times}4$ IDCT, HEVC $4{\times}4$ IDST, VP9 $4{\times}4$ IDCT, VP9 $4{\times}4$ IADST를 통합 하드웨어에서 처리가 가능하다. HEVC $4{\times}4$ IDCT와 VP9 $4{\times}4$ IDCT는 계수의 스케일만 다를 뿐 동일한 IDCT 연산을 사용하며, 마찬가지로 HEVC $4{\times}4$ IDST와 VP9 $4{\times}4$ IADST도 계수의 스케일만 다를 뿐 동일한 IDST 연산을 사용한다. 더욱이 IDCT 연산과 IDST 연산에는 상당히 많은 유사점이 있어 일부 하드웨어를 공동으로 사용할 수 있다. 따라서 제안하는 하드웨어는 4가지 연산에 대해 곱셈기의 계수는 각각 다르지만 버터플라이 덧셈기등은 공통으로 사용하여 통합적으로 수행한다. 0.18um 공정에서 합성했을 때 게이트 수가 약 6,679 게이트로 기존 아키텍처 대비 25.3% 감소함을 확인하였다.

HEVC 및 VP9 겸용 통합 역변환기의 설계 (Design of Unified Inverse Transformer for HEVC and VP9)

  • 정슬기;이성수
    • 전기전자학회논문지
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    • 제19권4호
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    • pp.596-602
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    • 2015
  • 본 논문에서는 HEVC와 VP9 겸용의 통합 역변환기를 설계하였다. 제안하는 아키텍처는 $4{\times}4$부터 $32{\times}32$ 크기의 HEVC IDCT, $4{\times}4$ 크기의 HEVC IDST, $4{\times}4$부터 $32{\times}32$ 크기의 VP9 IDCT, $4{\times}4$부터 $16{\times}16$ 크기의 VP9 IADST, $4{\times}4$ 크기의 IWHT까지 모든 모드의 계수 변환을 통합 역변환기에서 처리가 가능하다. HEVC와 VP9의 IDCT는 계수의 스케일만 다를 뿐 동일한 연산을 사용하며, HEVC의 $4{\times}4$ IDST와 VP9 $4{\times}4$ IADST 또한 계수의 스케일만 다를 뿐 동일한 연산을 사용한다. 더욱이 HEVC IDCT, VP9 IDCT, VP9 IADST 또한 상위 수준 IDCT의 서브셋이다. 제안하는 아키텍처는 연산이 같은 경우 곱셈기를 재사용하고 계수가 다를 경우에도 덧셈기 및 버터플라이 구조등을 최대한 공유함으로써 하드웨어의 크기를 크게 줄였다. 0.18 um 공정에서 합성했을 때 게이트 수가 456,442 게이트로 기존 아키텍처 대비 22.6% 감소하였다.

가교 폴리(4-비닐피리딘)-구리(II) 착물에 의한 Ascorbic Acid의 산화반응 2. 가교제의 영향 (Oxidation of Ascorbic Acid by Crosslinked Poly(4-vinyl pyridine)-Cu(II) Complexes 2. Effect of Crosslinker)

  • 이석기;서재곤;구광모;전일련;김우식
    • 폴리머
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    • 제24권2호
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    • pp.252-258
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    • 2000
  • 4-비닐피리딘과 N,N'-1,6-헥사메틸렌비스아크릴아미드를 라디칼중합하여 여러 가지 가교도를 가지는 가교 폴리(4-비닐피리딘) (CHP4VP)을 합성하였고, 이들 가교고분자와 구리(II)와의 착물을 평형흡착법으로 제조하였다. 제조한 착물들을 ascorbic acid (AA)의 산화반응 촉매로 사용하여 촉매활성을 조사하였다. CHP4VP-Cu(II) 착물에 의한 AA 산화반응은 Michaelis-Menten형 동력학적 거동을 나타내었다. CHP4VP-Cu(II) 촉매계의 촉매활성은 CHP4VP의 가교도가 증가할수록 증가하였고, 또 CHP4VP-Cu(II) 착물은 재사용 후에도 촉매활성이 거의 감소하지 않았다. 그러나 CHP4VP-Cu(II) 착물은 전보의 N,N'-메틸렌비스아크릴아미드가 가교제로 포함된 가교 폴리(4-비닐피리딘)-구리(II) 착물보다 AA 산화반응에 대한 촉매활성이 감소하는 경향을 나타내었다. 이들 결과로 부터 촉매계에 포함된 CHP4VP의 가교도와 가교제의 소수성이 AA 산화반응에 중요한 역할을 함을 알 수 있었다.

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P4VP과 PDP로 이루어진 Molecular Bottle-brush의 Order-Disorder Transition에 미치는 P4VP 분자량의 영향에 관한 연구 (Effects of Molecular Weight of Poly(4-vinylpyridine) on the Order-Disorder Transition of Molecular Bottle-brush Composed of Poly(4-vinylpyridine) and 3-Pentadecylphenol)

  • 최종렬;조항규;전현애;노시태
    • 폴리머
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    • 제24권4호
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    • pp.488-498
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    • 2000
  • 주쇄로 poly(4-vinylpyridine) (P4VP)과 양친매성 측쇄로 3-pentadecylphenol (PDP)의 수소결합을 통해서 molecular bottle-brush를 제조하였다. 제조된 bottle-brush에 대하여 P4VP의 pyridine기 대 PDP의 몰비 (x)와 P4VP 분자량에 따른 액정성의 구조, 상전이온도( $T_{ODT}$)와 bottle-brush 층간거리 ( $L_{p}$)변화에 대하여 고찰하였다. P4VP-PD $P_{x}$ bottle-brush는 상온영역에서 미세상분리를 이루고 있는 라멜라구조로 조사되었다. Bottle-brush의 온도에 따른 상거동에 대한 조사에서 상한임계온도(UCST)거동을 나타내었다. 또한 P4VP의 분자량별로 x가 0.8-0.9일때 최대온도를 나타내었으며, P4VP의 분자량이 증가함에 따라서 상전이가 높은 온도에서 일어났다. 이러한 결과는 주쇄긴 P4VP의 유동성과 라멜라구조의 크기와 규칙성에 영향을 받음을 알 수 있었다. Bottle-brush의 라멜라구조 분석시 bottle-brush의 $L_{p}$는 35 $\AA$에서 40 $\AA$로 x보다는 P4VP의 분자량에 더 큰 영향을 받았다. P4VP의 분자량이 증가함에 따라서 $L_{p}$도 증가하였으나, 일정 크기이상으로 분자량이 커졌을 경우, 오히려 거리가 감소하거나 증가하지 않는 결과를 얻었다.결과를 얻었다.다.

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균일한 자성 고분자 입자의 합성 (Synthesis of Monodispersed Magnetic Polymer Particle)

  • 안병현
    • 공업화학
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    • 제19권3호
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    • pp.316-321
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    • 2008
  • Styrene과 4-vinylpyridine의 공중합체(poly(st-co-4vp)) 입자를 무유화제 유화중합에 의해 합성하고 이 입자 내부에 iron pentacarbonyl을 침투시킨 후 열분해 시켜 입자의 표면과 내부에 산화철 나노입자를 갖는 자성 poly(st-co-4vp) 입자를 제조하였다. 얻어진 자성 poly(st-co-4vp) 입자의 크기와 분포, 열적 특성 및 자성 특성에 대해서 조사하였다. 자성 poly(st-co-4vp) 입자는 크기가 약 250 nm 정도로 매우 균일하였으며 약 14%의 산화철을 가졌다. 산화철은 XRD 시험에 의해 대부분 $Fe_3O_4$임이 확인되었으며 superconducting susceptometer (SQUID) 시험에 의해 자성 poly(st-co-4vp) 입자가 초상자성을 가짐을 확인하였다.

면섬유(綿纖維)에의 MMA/4-Vinylpyridine의 공(共)그라프트 중합(重合) (Graft Copolymerization of MMN4-Vinylpyridine onto Cotton Fiber)

  • 배현숙;김성련
    • 한국의류학회지
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    • 제17권3호
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    • pp.347-358
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    • 1993
  • Graft copolymerization of MMN4-VP onto cotton fiber using Ce(IV) salt as an initiator and triton X-100 as an emulsifier was performed under various polymerization conditions. In cograft polymerization, the polymeization behavior according to variation of 4-VP feed composition and the characteristics of MMA/4-VP graft polymer such as affinity for acid dye owing to cationization of cotton, antibacterial activity and thermal behavior were investigated. The results of this study were as follows : 1. While in copolymerization of MMA and 4-VP, 4-VP content in copolymer was more than that of monomer feed composition. 2. Increasing 4-VP content, graft yield was decreased, but graft efficiency was increased. In case of MMA/4-VP graft polymerization, the highest graft yield was obtained at higher CAN concentration than in MMA graft polymerization, the reason is that the behavior of 4-VP was disturbed by Ce(IV) sail 3. Elevation of temperature resulted in increase of graft yield and the apparent activation energy of MMA/4-VP graft polymerization was higher than that of MMA graft polymerization. 4. MMA/4-VP grafted cotton fiber showed affinity for acid dye, antibacterial activity and higher moisture regain than MMA grafted cotton fiber. MMA/4-VP grafted cotton fabric showed improvement of wrinkle recovery up to 40~50% graft yield and decreased thereafter. MMA/4-VP and MMA grafted cotton fabric did not showed significant difference in wrinkle recovery and stiffness.

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IBDV에 대한 단크론항체 생산 및 진단적 응용 (Production and diagnostic application of monoclonal antibodies against infectious bursal disease virus)

  • 류민상;송윤기;이승철;모인필;강신영
    • 한국동물위생학회지
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    • 제34권1호
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    • pp.5-12
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    • 2011
  • Infectious bursal disease (IBD) caused by infectious bursal disease virus (IBDV) is a highly contagious viral disease in chicken. It causes heavy economic loss by immune suppression and high mortality. The IBDV, designated Avibirnavirus in the Family Birnaviridae, has a double-stranded RNA genome formed by two segments, segment A and segment B. Segment A encodes a 108 KDa polypeptide that is self-cleaved to produce pVP2, VP3 and VP4, and later pVP2 is cleaved to VP2. The VP2 contains the antigenic regions responsible for elicitation of neutralizing antibodies and VP3 is a major immunogenic protein of IBDV. In this study, monoclonal antibodies (MAbs) specific for IBDV were produced and characterized. All 15 MAbs were specific for IBDV and did not react with other viruses used in this study. The protein specificity of MAbs was determined by comparing the reactivity patterns of each MAb with IBDV VP2 and VP234 recombinant baculoviruses and Western blot analysis. As a result, 7 MAbs (1F5, 2C8, 2F4, 3C7, 4C3, 6F11, 6G5) and 5 MAbs (2A4, 2G2, 3F5, 3G2, 4F10) were specific for VP2 and VP3, respectively. The protein specificity of 3 MAbs (2B8, 3F7, 3F8) were not determined. Five (2C8, 2F4, 4C3, 6F11, 6G5) of the VP2-specific MAbs had a neutralizing activity against IBDV. Some MAbs reacted with IBDV-infected bursa of Fabricius by indirect fluorescence antibody (IFA) and immunohistochemistry (IHC) assay. The MAbs produced in this study would be used for diagnostic reagents for the detection of IBDV infection.

Genetic characterization of bovine rotavirus isolates in Korea

  • Yang, Dong-Kun;Kim, Byoung-han;Lee, Kyung-Woo;Kim, Yeon-Hee;Song, Jae-Young;Park, Joong-Won;Son, Seong-Wan
    • 대한수의학회지
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    • 제48권4호
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    • pp.423-429
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    • 2008
  • Throughout the world, rotavirus infections cause extensive morbidity in human infants and diarrhea in animals such as white scour caused by bovine rotavirus in calves. We isolated three rotavirus strains designated KV0407, KV0418, and KV0426 from 103 fecal samples of diarrheic calves. The genes coding for proteins VP4, VP6, VP7, and NSP4 from strain KV0407 were sequenced and compared with the nucleotide sequences of other known strains of rotavirus. The KV0407 VP4 gene was highly homologous to the OSU (99.4%) and JL94 (99.4%), but not the B223 (62.4%) and K33 (62.4%) VP4 genes. The KV0407 and KV0418 VP7 genes were most similar to the OSU and super-short type VMRI VP7 genes. Based on nucleotide sequence analysis, the KV0407 strain was tentatively assigned to A serogroup (SG I), G5P[7], NSP4 genotype B and the KV0418 and KV0426 strains were assigned to A serogroup (SG I), G6P[5], NSP4 genotype A. The genetic characterization of these bovine rotavirus isolates could be useful for the diagnosis and prevention of diarrhea in calves.

디지털흉부X선촬영에서 검출기 방식에 따른 최적의 노출조건 (Optimal Exposure Conditions according to Detector Type in Chest Digital Radiography)

  • 이원정
    • 대한안전경영과학회지
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    • 제17권4호
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    • pp.213-221
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    • 2015
  • The aim of this study was to set up the optimal exposure condition according to detector type considering image quality (IQ) with radiation dose in chest digital radiography. We used three detector type such as flat-panel detector (FP) and computed radiography (CR), and charge-coupled device (CCD). Entrance surface dose (ESD) was measured at each exposure condition combined tube voltage with tube current using dosimeter, after attaching on human phantom, it was repeated 3 times. Phantom images were evaluated independently by three chest radiologists after blinding image informations. Standard exposure condition using each institution was 117 kVp-AEC at FP and 117 kVp-8 mAs at CR, and 117 kVp-8 mAs at CCD. Statistical analysis was performed by One way ANOVA (Dunnett T3 test) using SPSS ver. 19.0. In FP, IQ scores were not significant difference between 102 kVp-4 mAs and 117 kVp-AEC (28.4 vs. 31.1, p=1.000), even though ESD was decreased up to 50% ($62.3{\mu}Gy$ vs. $125.1{\mu}Gy$). In CR, ESD was greatly decreased from 117 kVp-8 mAs to 90 kVp-8 mAs without significant difference of IQ score (p=1.000, 24.6 vs. 19.5). In CCD, IQ score of 117 kVp-8 mAs was similar with 109 kVp-8 mAs (29.6 vs. 29.0), with decreasing from $320.8{\mu}Gy$ to $284.7{\mu}Gy$ (about 11%). We conclude that optimal x-ray exposure condition for chest digital radiography is 102 kVp-4 mAs in FP and 90 kVp-8 mAs in CR, and 109 kVp-8 mAs in CCD.