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http://dx.doi.org/10.7853/kjvs.2013.36.1.23

Molecular characterization of avian rotavirus isolated in Korea  

Wang, Jun-Hui (College of Veterinary Medicine, Chungbuk National University)
Koo, Bon-Sang (College of Veterinary Medicine, Chungbuk National University)
Mo, In-Pil (College of Veterinary Medicine, Chungbuk National University)
Kang, Shien-Young (College of Veterinary Medicine, Chungbuk National University)
Publication Information
Korean Journal of Veterinary Service / v.36, no.1, 2013 , pp. 23-30 More about this Journal
Abstract
An avian rotavirus (AvRV-2) was isolated from feces of broilers suffering from acute gastroenteritis in 2011. It was the first avian rotavirus isolated in Korea. To investigate the molecular characteristics of AvRV-2, the VP4, VP6, VP7 and NSP4 gene nucleotide sequences were determined and compared with those of rotavirus strains available in the GenBank database. The phylogenetic tree of VP7 gene showed that AvRV-2 had a high degree of nucleotide sequence homology (93.4% to 94.7%) with those of rotaviruses belonging to genotype G19 cluster. The phylogenetic tree of the VP4 gene revealed a high degree of nucleotide sequence homology (95.8% to 95.9%) with genotype P[30] rotaviruses isolated from chickens. The VP6 and NSP4 gene nucleotide sequences showed the highest identities with those of avian strains with 95.3% to 96.4% and 90.3% to 92.2%, respectively. Genetic characterization of the VP4, VP6, VP7 and NSP4 showed that AvRV-2 strain was most closely related to chicken rotavirus strains from Germany and Japan. Comparative nucleotide sequences and phylogenetic analysis indicated that avian rotavirus isolated from broilers belonged to genotype G19P[30] and it was the first report on avian rotavirus infection in Korea.
Keywords
Avian rotaviruses; VP4; VP6; VP7; NSP4 gene;
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1 Ball JM, Tian P, Zeng CQ, Morris AP, Estes MK. 1996. Age-dependent diarrhea induced by a rotaviral nonstructural glycoprotein. Sci 272: 101-104.   DOI   ScienceOn
2 Bellinzoni R, Mattion N, Vallejos L, La Torre JL, Scodeller EA. 1987. Atypical rotavirus in chickens in Argentina. Res Vet Sci 43: 130-131.
3 Bergeland ME, McAdaragh JP, Stotz I. 1977. Rotaviral enteritis in turkey poults. Proc 26th West Poult Dis Conf: 129-130.
4 Elschner M, Hotzel H, Reetz J, Diller R, Otto P. 2005. Isolation, identification and characterization of group A rotavirus from a chicken: the inner capsid protein sequence shows only a distant phylogenetic relationship to most other avian group A rotaviruses. J Vet Med Infect Dis Vet Publish Health 52: 211-213.   DOI   ScienceOn
5 Estes MK, Kapikian AZ. 2007. Rotaviruses. pp. 1917-1957. In: Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA , Roizman B, Straus SE(ed.). Fields Virology. 5th ed. Lippincott Williams and Wilkins, Pennsylvania.
6 Gorziglia M, Larralde G, Kapikian AZ, Chanock RM. 1990. Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci 87: 7155-7159.   DOI   ScienceOn
7 Gusmal RH, Mascarenhas JD, Gabbay YB, Linhares AC. 1994. Nosocomial transmission of an avian-like rotavirus strain among children in Belem, Brazil. J Diarrhoeal Dis Res 12: 129-132.
8 Hines ME, Styer EL, Baldwin CA, Cole JR Jr. 1995. Combined adeovirus and rotavirus enteritis with Escherichia coli septicemia in an emu chick (Dromaius novaehollandiae). Avian Dis 39: 646-651.   DOI   ScienceOn
9 Ito H, Sugiyama M, Masubuchi K, Mori Y, Minamoto N. 2001. Complete nucleotide sequence of a group A avian rotavirus genome and a comparison with its counterparts of mammalian rotaviruses. Virus Res 75: 123-138.   DOI   ScienceOn
10 Jeong YJ, Park SI, Hosmillo M, Shin DJ, Chun YH, Kim HJ, Kwon HJ, Kang SY, Woo SK, Park SJ, Kim GY, Kang MI, Cho KO. 2009. Detection and molecular characterization of porcine group C rotaviruses in South Korea. Vet Microbiol 138: 217-224.   DOI   ScienceOn
11 Kang SY, Nagaraja KV, Newman JA. 1986a. Primary isolation and identification of avian rotaviruses from turkeys exhibiting signs of clinical enteritis in a continuous MA104 cell line. Avian Dis 30: 494-499.   DOI   ScienceOn
12 Kang SY, Nagaraja KV, Newman JA. 1986b. Electropherotypic analysis of rotaviruses isolated from turkeys. Avian Dis 30: 794-801.   DOI   ScienceOn
13 Kang SY, Saif LJ, Miller KL. 1989. Reactivity of VP4-specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotypes of human(symptomatic and asymptomatic) and animal rotaviruses. J Clin Microbiol 27: 2744-2750.
14 Matthijnssens J, Ciarlet M, McDonald SM, Attoui H, Banyai K, Brister JR, Buesa J, Esona MD, Estes MK, Gentsch JR, Iturriza-Gomara M, Johne R, Kirkwood CD, Martella V, Mertens PP, Nakagomi O, Parreno V, Rahman M, Ruggeri FM, Saif LJ, Santos N, Steyer A, Taniguchi K, Patton JT, Desselberger U, Van Tanst M. 2011. Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Arch Virol 156: 1397-1413.   DOI   ScienceOn
15 Meulemans G, Peeters JE, Halen P. 1985. Experimental infection of broiler chickens with rotavirus. Br Vet J 141: 69-73.   DOI
16 McNulty MS, Allan GM, Stuart JC. 1978. Rotavirus infection in avian species. Vet Rec 103: 319-320.
17 McNulty MS, Allan GM, Todd D, McFerran JB, McCracken RM. 1981. Isolation from chickens of a rotavirus lacking the rotavirus group antigen. J Gen Virol 55: 405-413.   DOI   ScienceOn
18 McNulty MS, Reynolds DL. 2008. Rotavirus Infections. pp. 338-350. In: Saif YM, Fadly AM, Glisson JR, Mc-Dougald LR, Nolan LK, Swayne DE(ed.). Diseases of Poultry. 12th ed. Blackwell publishing, Oxford.
19 Minamoto N, Oki K, Kinjo T, Suzuki Y. 1988. Isolation and characterization of rotavirus from feral pigeon in mammalian cell cultures. Epidemiol Infect 100: 481-492.   DOI
20 Oh BH, Moon SJ, Lee KS. 1983. Clinical, laboratory and epidemiological features of human rotavirus (HRV) gastroenteritis. Kor J Pediatr Soc 26: 959-966.
21 Otto P, Schulze P, Herbst W. 1999. Demonstration of group C rotaviruses in fecal samples of diarrheic dogs in Germany. Arch Virol 144: 2467-2473.   DOI   ScienceOn
22 Park SH, Saif LJ, Jeong C, Lim GK, Park SI, Kim HH, Park SJ, Kim YJ, Jeong JH, Kang MI, Cho KO. 2006. Molecular characterization of novel G5 bovine rotavirus strains. J Clin Microbiol 44: 4101-4112.   DOI   ScienceOn
23 Park SI, Matthijnssens J, Saif LJ, Kim HJ, Park JG, Alfajaro MM, Kim DS, Son KY, Yang DK, Hyun BH, Kang MI, Cho KO. 2011. Reassortment among bovine, porcine and human rotavirus strains results in G8P[7] and G6P[7] strains isolated from cattle in Korea. Vet Microbiol 152: 55-66.   DOI   ScienceOn
24 Raming RF, Ciarlet M, Mertens PPC, Dermody TS. 2005. Rotavirus. pp. 484-496. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA(ed.). Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. Elsevier, California.
25 Wani SA, Bhat MA, Ishag SM, Ashrafi MA, Buchh AS, Haq M. 2003. Detection of a mammalian-like group A rotavirus in diarrhoeic chicken. Vet Microbiol 94: 13-18.   DOI   ScienceOn
26 Schumann T, Hotzel H, Otto P, Johne R. 2009. Evidence of inter-species transmission and reassortment among avian group A rotaviruses. Virology 386: 334-343.   DOI   ScienceOn
27 Takase K, Nonaka F, Sakaguchi M, Yamada S. 1986. Cytopathic avian rotavirus isolated from duck faeces in chicken kidney cell cultures. Avian Pathol 15: 719-730.   DOI
28 Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighing, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680.   DOI   ScienceOn
29 Yason CV, Schat KA. 1985. Isolation and characterization of avian rotaviruses. Avian Dis 29: 499-508.   DOI   ScienceOn
30 Yason CV, Schat KA. 1986. Pathogenesis of rotavirus infection in turkey poults. Avian Pathol 15: 421-435.   DOI