• Title/Summary/Keyword: VIMS

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Application of multi dimensional NMR experiments to VBS RNAs of Yeast Saccaromyces cerevisiae virus

  • Chaejoon Cheong;Cheong, Hae-Kap;Yoo, Jun-Seok
    • Journal of the Korean Magnetic Resonance Society
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    • v.5 no.1
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    • pp.29-36
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    • 2001
  • The structures of two VBS (viral binding site) RNAs, SL1 and SL2, of Yeast Saccharomyces cerevisiae vims have been studied by 2D and 3D NMR experiments. VBSs play a crucial role in viral particle binding to the plus strand and packaging of the RNA. The secondary structures of the two VBS RNAs share a common feature of the stem-internal loop-stem-hairpin loop structure although the size of the internal loops of SL1 and SL2 differs. 2D experiments were sufficient for fill assignments of SL1. However, isotope labeling of the sample and multidimensional experiments were required for 28-nucleotide-long SL2 due to the spectral overlap. Several 3D HCCH experiments have accomplished full assignment of SL2 RNA.

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Simulating the 3.4-Micron Feature of Titan's Haze

  • Kim, Y.S.;Ennis, C.;Kim, Sang Joon
    • Bulletin of the Korean Chemical Society
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    • v.34 no.3
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    • pp.759-762
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    • 2013
  • Four prominent features of Titan's haze are found within the '3.4-${\mu}m$' absorption to be uniform with recent vertically resolved Cassini/VIMS spectra. These are absorptions at 2998 $cm^{-1}$ (3.34 ${\mu}m$), 2968 $cm^{-1}$ (3.37 ${\mu}m$), 2927 $cm^{-1}$ (3.42 ${\mu}m$), and 2882 $cm^{-1}$ (3.47 ${\mu}m$). A detailed fitting suggests that the 2998 $cm^{-1}$ feature could originate from amorphous acetonitrile ($CH_3CN$) carrying about 25% of integrated optical depth; the remaining features, which account for 75% of the integrated optical depth, could arise from a distinct triplet (C-H stretching) structure of radiolyzed hydrocarbons. An additional feature was possibly evidenced at altitudes higher than 300 km and attributable to 'polymer-capped' methane ($CH_4$), significantly constraining the chemical composition of organic haze layers under Titan's active radiation field.

타이탄의 2-3 micron 영역 스펙트럼에 나타나는 대기 중 입자에 의한 흡수밴드와 고체 탄화수소화합물 흡수밴드 모델의 비교

  • Sim, Chae-Gyeong;Kim, Sang-Jun
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.1
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    • pp.102-102
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    • 2012
  • 타이탄의 근적외선 영역 스펙트럼에서 나타나는 특유의 넓은 흡수밴드를 고체 탄화수소화합물과 질소화합물의 조합으로 설명하고자 한다. 이 흡수 밴드는 대기권의 하층부에 존재하는 연무(haze)에 의한 것으로, 3.3 - 3.4 micron 부근과 2.30 - 2.35 micron 부근에서 유사한 형태가 발견됐다. 두 파장대 모두 C-H 스트레칭 모드가 존재하는 영역이라는 공통점이 있으며, 그 형태가 넓은 흡수밴드로 나타나므로 대기 중에 고체상태의 탄화수소화합물이 존재하는 것으로 유추할 수 있으며, 관련 분자들의 실험값과 복사전달모델을 이용하여 이를 설명하고자 한다. 또한, 이 흡수밴드는 고도에 따라 그 형태와 세기가 달라지므로, 연무 입자들의 고도에 따른 수직분포 및 크기 등을 파악할 수 있다. 이 연구에서는 기존에 타이탄에서 발견된 $CH_4$, $C_2H_6$, $CH_3CN$의 고체 분광선과, 해당 영역에서 흡수선을 보이는 $C_5H_{12}$, $C_6H_{12}$, $C_6H_{14}$ 등의 고체 분광선을 이용한 모델을 Cassini 탐사선의 VIMS 관측자료로부터 유도한 파장 및 고도에 따른 광학적 깊이 변화량과 비교하여 보여주고자 한다.

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타이탄 3 마이크론 영역에서 보이는 유기 화합물의 미확인 흡수 밴드 연구

  • Jeong, Ae-Ran;Kim, Sang-Jun
    • The Bulletin of The Korean Astronomical Society
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    • v.35 no.1
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    • pp.89.1-89.1
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    • 2010
  • Cassini/VIMS 관측에 의하면 타이탄의 3 마이크론 파장영역에서 다른 파장 대와 달리 메탄 분자만으로는 관측 값에 맞는 모델을 만들기 힘든 특이한 형태의 흡수 밴드 영역이 발견되었다 (Bellucci et al. 2009, Icarus, v. 201, p. 198). 이 파장영역은 행성뿐만 아니라 ISM (Interstellar Medium)과 혜성 등에서 연구 되고 있는 C-H stretching band와 흡사한 구조를 가지고 있다. 이러한 구조는 타이탄 연무 속에 포함된 유기물질에 의한 것으로 추정된다. 본 연구는 최근까진 개발된 복사방정식 모델과 2006년 Cassini/VIMS가 관측한 고도에 따른 solar occultation 데이터를 비교하여 밴드구조의 실체를 알아보는 것이 주 목적이다. 우리는 고도 별로 다르게 나타나는 흡수밴드의 파장 영역을 세밀히 나눈 후 메탄과 함께 유기물질의 연무가 더해진 모델을 만들었다. 도출된 유기물질의 구조가 관측된 밴드 구조에 미치는 영향을 알아보고 앞으로의 연구 방향을 제시 할 것이다.

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Studies on the immunization against Newcastle disease virus in Ogol chicken (오골계의 뉴캣슬바이러스 면역원성 조사)

  • 이종훈;김영진;정영균;정영재
    • Korean Journal of Veterinary Service
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    • v.22 no.1
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    • pp.37-42
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    • 1999
  • This study was conducted to determine whether the vaccination programs for the control of Newcastle disease(ND) would affect the immune status of Ogol chicken in Yeonsan, Chungnam province. The results obstained are summerized as followes ; 1. Maternal antibody titers level of Ogol chickes was 2 $log_2$. 2. The efficacy of the Newcastle spray and drinking water vaccine was evaluated by the antibody responses and the protection rates(over 90% ) after challenge with a virulent Newcastle disease vims at 14 days. 3. Spray vaccine caused on visible side reaction like respiratory symptoms, but it is declined of the 4~5 days.

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Low Resolution Near-Infrared Stellar Spectra Observed by CIBER

  • Kim, MinGyu;Lee, Hyung Mok
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.1
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    • pp.76.2-76.2
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    • 2016
  • We present near-infrared (0.8 - 1.8 microns) spectra of 63 bright (J_mag < 10) stars observed with Low Resolution Spectrometer (LRS) onboard the rocket-borne Cosmic Infrared Background Experiment (CIBER). Two Micron All Sky Survey (2MASS) photometry information is used to find cross-matched stars after reduction and extraction of the spectra. We identify the spectral types of observed stars by comparing with spectral templates from the Infrared Telescope Facility (IRTF) library. All the observed spectra are consistent with late F to M stellar spectral types, and we identify various infrared absorption lines. As our observations are performed above the Earth's atmosphere, our spectra are free from telluric contamination. Including HST/NICMOS and Cassini/VIMS, the spectral coverage has rarely been achieved in space, and the methods developed here can inform statistical studies with future low-resolution spectral measurements such as GAIA photometric and radial velocity spectrometer.

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A Study on KOREA SCMS Analysis and Improvement Method (한국 V2X 보안인증체계 분석 및 개선방향 연구)

  • Eom, Sungwook;Kim, Donghwan;Kim, Sungsub;Cho, Sungwoo
    • Journal of Auto-vehicle Safety Association
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    • v.13 no.1
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    • pp.31-37
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    • 2021
  • SCMS is a security credential management system for V2X communication, which performs generation/ provision/validation of device's security certificates. In this paper, we will explain about the main functions of SCMS and the role of each institution, and propose the following improvement measures in the process of establishing the Korean V2X security certification system. First, connection scheme of ERA (Enrollment certificate RA) between SCMS and Vehicle Manager Information System (VIMS) will be proposed. Second part is the problem of certificate revocation and proposal of improvements.

Production of Hantaan Virus from Human Immortalized Retina Cell and Its Immunogenicity

  • Bae, Cheon-Soon;Choi, Jun-Youl;An, Chang-Nam;Kim, Jong-Su;Hur, Byung-Ki
    • Journal of Microbiology and Biotechnology
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    • v.12 no.6
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    • pp.882-889
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    • 2002
  • Hantaan vims production, using human immortalized retina cell (PER. C6), was investigated to develop an inactivated virus vaccine. To infect Hantaan virus into PER. C6, two infection methods (medium-to-cell and cell-to-cell) were tried, and IFA results showed that the cell-to-cell infection method was very useful for producing Hantaan virus-infected PER, C6. Hantaan virus production was significantly affected by the growth rate of PER. C6 and the content of FBS in medium. Higher specific growth rate of infected PER. C6 and lower FBS content induced higher production of Hantaan virus. The inactivated human cell-culture vaccines with various EIA titers were prepared, their antibody responses were compared with those of inactivated suckling mouse brain vaccines ($Hantavax^처리불가$). and the result showed their immunogenicities were slightly higher than those of inactivated suckling mouse vaccines. Therefore, this study shows the possibility of the development of Hantaan virus vaccine from a human cell culture.

Enterotoxemia caused by Clostridium perfringens type A in Formosan deer (꽃사슴의 Clostridium perfringens A형에 의한 장독혈증 발생 보고)

  • 이청산;한성태;곽학구;박경재;현공율;조우영;이종인;배유찬;진영화
    • Korean Journal of Veterinary Service
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    • v.25 no.2
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    • pp.127-133
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    • 2002
  • The case reports for clostridium type A enterotoxemia in Formosan deer have rarely been reported. This paper describes a natural case of type A enterotoxemia in farmed Formosan deer in Cheongwon-gun. A dead, male 10-month-old Formosan deer was submitted to Chungbuk Livestock and Veterinary Research Institute, March 24, 2001 and examined. That deer was fed with assorted grain feed, oak leaves, acorn and bean curd. Grossly there was no visible external change. Despite of the carcass being examined within 12 hours of death, there was a quite degree of posonortem decomposition. There was severe hemorrhage in the serosa of abomasum and small intestine. Much blood tinged and watery contents were contained in those organs. Also there were severe swelling of spleen, some red foci in hepatic parenchyma. Microscopically there were severe congestion and hemorrhage in mucosa submucosa, muscular layer, and serosa of abomasum and small intestine. Also spleen and pancreas showed severe Congestion and hemorrhage. There were multifocal hemorrhage with hepatic necrosis in periportal area and focal mononuclear cell deposition in sinusoid. In bacterial culture for small intestine, Cl perfringens was isolated. By toxin typing for the strain, that had $\alpha$ -toxin belonged to type A. In electronmicroscopy for feces, no vims particle was detected. Considering clinical signs, gross lesions, microscopic lesions, bacterial culture, and toxin typing of the isolate, this case was diagnosed as enterotoxemia by Cl perfringens type A.

Detection of Cytomegalovirus in Atherosclerotic Aorta and Coronary Artery by In Situ Hybridization and PCR

  • Eom, Yong-Bin;Kwon, Tae-Jung;Lee, Sang-Yong;Lee, Won-Tae;Kim, Jong-Bae
    • Biomedical Science Letters
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    • v.8 no.4
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    • pp.257-268
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    • 2002
  • Chronic infection and inflammation have recently been implicated as important etiologic agents of atherosclerosis. Several agents have been suggested as possible candidates including cytomegalovirus (CMV), herpes simplex vims type 1 (HSV-1), Epstein-Barr virus (EBV), Chlamydia pneumoniae, and Helicobacter pylori. We evaluated the relationship between cytornegalovirus infection and atherosclerosis by in situ hybridization and polymerase chain reaction (PCR). We examined 23 subjects with atherosclerosis and 10 matched control subjects without atherosclerosis. CMV was detected by in situ hybridization in 60.9% (14/23) of aorta and 42.9% (9/21) of coronary arteries in subjects with atherosclerosis. It was also detected by PCR in 65.2% (15/23) of aorta and 52.4% (11/21) of coronary arteries. CMV was detected on areas showing early or advanced atheromatous changes. Cells morphologically identical to smooth muscle cells, endothelial cells, lymphocytes, fibroblasts, and Schwann cells were positively reacted with the CMV probe. However. none of the cells to which the probe hybridized contained inclusion bodies, thus strongly suggesting that the arterial wall may be a site of CMV latency. This result Indicates that CMV may potentially play a direct or indirect role in the pathogenesis of human atherosclerosis.

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