• Title/Summary/Keyword: VAM

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The Presence and Importance of VAM Spores in the Soil of Ginseng Gardens

  • Weber, Hans-Christian
    • Proceedings of the Ginseng society Conference
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    • 1998.06a
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    • pp.90-95
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    • 1998
  • Symbiotic associations between land plants and fungi have been known for more than one hundred years. Vesiculararbuscular mycorrhizas (VAM) are the most common symbiosis in flowering plants and can be recognized in almost all plant families. These fungal associations play a very important role in the growth and survival of plant species. However, with respect to the importance and intensity of the VAM, there is great variation among host species. Our knowledge of the VAM fungus-plant association in Araliaceae is very limited. After the first reports of the occurence of VAM in lateral roots of Panax species, mycorrhizal structures are now described as special structures representing the so-called Paris type. In this type, the development of new spores and vesicles is extremely low. This and the type of colonization of the fungus in Panax roots indicates on, one hand, the high intensity of the VAM and, on the other hand, a remarkable dependency for VAM in members of the Panax species. Therefore, it can be easily understood that cultivated Panax plants exhibit a significant uptake of nutrients and this leads to an extremely depleted soil at harvest. Further, the soil is nearly free of the spores of VAM fungi as they germinate each year on the newly developing Panax roots.

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Occurrence and Quantification of Vesicular-Arbuscular Mycorrhizal (VAM) Fungi in Industrial Polluted Soils

  • SELVARAJ;THANGASWAMY;PADMANABHAN CHELLAPPAN;JEONG, YU-JIN;KIM, HOON
    • Journal of Microbiology and Biotechnology
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    • v.15 no.1
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    • pp.147-154
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    • 2005
  • A survey for vesicular-arbuscular mycorrhizae (VAM) status was undertaken in three different industrially polluted sites at Uyyakondan channel of Senthanneerpuram area in Trichy, India. The soils and the effluents were acidic, and contained higher Zn (621 to 711 ppm) than the other heavy metals, such as Cu, Pb, and Ni. Eighteen plant species were collected from the rhizosphere soils, and 13 species were positive for VAM colonization. Fifteen VAM fungal species were isolated from the plant species. The number of VAM fungal spores from the soils ranged from 45 to 640 per 100 g of soil. There was a significant correlation observed between the number of spores and percentage root colonization, as exemplified by Acalypha indica (45 and 20%, respectively) and Paspalum vaginatum (640 and 98%, respectively). Hostspecific and site-specific associations were observed in site 2; particular VAM species, Gigaspora gigantea and Glomus fasciculatum, were specific to particular host plants, Phyllanthus maderaspatensis and A. indica, respectively, even though Eclipta prostrata and Physalis minima were maximally associated with 8 VAM species. G. fasciculatum was found in 11 plant species and predominant VAM species. These results led us to conclude that VAM fungi are associated with a majority of the plants in the industrial polluted sites and support the plants to survive in the acidic soils, polluted with heavy metals of the industrial effluents.

Enhanced Internalization of Macromolecular Drugs into Mycobacterium smegmatis with the Assistance of Silver Nanoparticles

  • Sun, Fangfang;Oh, Sangjin;Kim, Jeonghyo;Kato, Tatsuya;Kim, Hwa-Jung;Lee, Jaebeom;Park, Enoch Y.
    • Journal of Microbiology and Biotechnology
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    • v.27 no.8
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    • pp.1483-1490
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    • 2017
  • In this study, silver nanoparticles (AgNPs) were synthesized by the citrate reduction process and, with the assistance of n-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, were successfully loaded with the macromolecular drug vancomycin (VAM) to form AgNP-VAM bioconjugates. The synthesized AgNPs, VAM, and AgNP-VAM conjugate were characterized by UV-visible spectroscopy, zeta potential analysis, confocal microscopy, and transmission electron microscopy. The effect of loading VAM onto AgNPs was investigated by testing the internalization of the bioconjugate into Mycobacterium smegmatis. After treatment with the AgNP-VAM conjugate, the bacterial cells showed a significant decrease in UV absorption, indicating that loading of the VAM on AgNPs had vastly improved the drug's internalization compared with that of AgNPs. All the experimental assessments showed that, compared with free AgNPs and VAM, enhanced internalization had been successfully achieved with the AgNP-VAM conjugate, thus leading to significantly better delivery of the macromolecular drug into the M. smegmatis cell. The current research provides a new potential drug delivery system for the treatment of mycobacterial infections.

Occurrence of Vesicular-Arbuscular Mycorrhizal (VAM) Fungi and Their Effect on Plant Growth in Endangered Vegetations

  • Selvaraj, Thangaswamy;Padmanabhan, Chellappan;Jeong, Yu-Jin;Kim, Hoon
    • Journal of Microbiology and Biotechnology
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    • v.14 no.4
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    • pp.885-890
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    • 2004
  • A survey for vesicular-arbuscular mycorrhizae (VAM) occurrence was undertaken in three endangered vegetation sites in the area of Kudankulam atomic power station. Fifteen VAM fungal species were isolated from the root-zone soils of fourteen different plant species. There was a significant correlation observed between the number of spores and of percentage root colonization as exemplified by Phyllanthus niruri and Paspalum vaginatum (450, 95%; 60, 25%). Although VAM species are not known to be strictly site specific, the fact that Acaulospora elegans was observed only in site 1, Glomus pulvinatum in site 2 only, and Gl. intraradices in site 3 only, showed site-specificity in this study. To confirm the infection efficiency, two host plant species in the sites, P. niruri and Eclipta alba, were selected and inoculated in field with three selected VAM fungal spores. Gl. fasciculatum was found to be the most efficient VAM species in percentage root colonization, number of VAM spores, and dry matter content. When the nutrients in roots of P. niruri and E. alba were analyzed, there was higher uptake of K (4.2 and 3.4 times, respectively) and Ca (5.3 and 4.9 times, respectively), the analogues for $^{137}Cs$ and $^{90}Sr$, respectively. From the results, it might be concluded that VAM association helps the plants survive in a disturbed ecosystem and enhances uptake and cycling of radionuclides from the ecosystem.

Improvement in Diagnostic Accuracy of Hemangioma and Vascular Malformation through Multidisciplinary Clinic (협력진료를 통한 혈관종 및 혈관기형 진단 정확도 향상)

  • Kang, Kyung-Dong;Bae, Yong-Chan;Nam, Su-Bong;Choi, Soo-Jong;Jung, Do-Sang;Kim, Chang-Won
    • Archives of Plastic Surgery
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    • v.37 no.6
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    • pp.758-762
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    • 2010
  • Purpose: It is difficult to provide accurate diagnosis and proper treatment for vascular anomalies, because vascular anomalies have a wide spectrum of lesion with various symptoms. Furthermore, vascular anomalies often require the services of a wide range of specialists. So, many patients have been discouraged by the lack of expertise and treatment options available to them. In this study, we aimed to describe the scope of the Vascular Malformation clinic (VAM clinic), a multidisciplinary clinic for hemangioma and vascular malformation patients and investigate of diagnostic accuracy of VAM clinic. Methods: 68 patients have included in a retrospective, medical record review study in the VAM clinic from may 2002 to august 2009. Data included the types of diagnoses of patients seen in the clinic, locations of lesions, comparison of accuracy of diagnoses at other clinic and initial diagnoses at VAM clinic and types of recommended treatments. Results: Initial diagnosis at VAM clinic showed 94.2% accuracy, while diagnosis at other clinic only showed 12.5% accuracy. Head and neck is most frequently occurred lesions in patients of VAM clinic. And VAM clinic is able to provide various and proper treatments to patients. Conclusion: Hemangiomas and vascular malformations can present with various medical problems. VAM clinic, the multidisciplinary clinic provided accurate diagnoses and proper treatments to patients.

Video-Assisted Mediastinoscopic Surgery (VAM) (비디오 종격동경 수술)

  • Kim, Yong-Hee
    • Korean Journal of Bronchoesophagology
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    • v.16 no.1
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    • pp.5-10
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    • 2010
  • Objectives The purpose of this article was to review the video-assisted mediastinoscopic surgery (VAM) as diagnostic or therapeutic tools. Summary The VAM was indicated in diagnosis of pleural diseases, dissection or sampling of mediastinal or paraesophageal lymph nodes, nodal staging in lung cancer, or various therapeutic approaches. The advantages of VAM were included that was less painful, cosmetic merit, no need additional drain, and no effect of cardiopulmonary function. It was provided to surgeons wider and more detail operational field rather than conventional mediastinoscopic surgery. It was useful tool as training beginner surgeon, Although the VAM had limited indications, it would be feasible and important multi-purposal operational tools in mediastinal, pleural and lung diseases.

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Effect of Two Glomus Species Inoculations on Survival, Photosynthetic Capacity, Growth, Morphology ana Root Ginsenoside Content of Panax quinquefolius L.

  • Fournier, Anick R.;Khanizadeh, Shahrokh;Gauthier, Louis;Gosselin, Andre;Dorais, Martine
    • Journal of Ginseng Research
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    • v.27 no.4
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    • pp.178-182
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    • 2003
  • Vesicular-arbuscular mycorrhizae (VAM) fungi naturally colonise American ginseng roots and this relationship is highly beneficial to enhance plant productivity. Our goal was to determine the effect of adding two Glomus species (Glomus etuticatum, G. intraradices) on survival, photosynthetic capacity, growth, morphology and root ginsenoside content of one-year-old American ginseng plants grown in a broadleaf forest. While our study revealed that VAM inoculations significantly affected root morphology and Re ginsenoside content, the survival, photosynthetic capacity and root growth of American ginseng plants were not significantly influenced by VAM inoculations. Surface area and volume of rootlets were 16-25% higher for ginseng grown in VAM-inoculated soil compared to those grown in the control plots. Also, Re ginsenoside content was 18 % higher in YAM-inoculated roots compared to controls.

Use of Sucrose-Agar Globule with Root Exudates for Mass Production of Vesicular Arbuscular Mycorrhizal Fungi

  • Thangaswamy Selvaraj;Kim, Hoon
    • Journal of Microbiology
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    • v.42 no.1
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    • pp.60-63
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    • 2004
  • A sucrose-agar globule (SAG) was newly introduced to increase production of the vesicular arbuscular mycorrhizal (VAM) fungal spores, Gigaspora gigantea and Glomus fasciculatum. An SAG inoculum and a sucrose-agar globule with root exudates (SAGE) inoculum were prepared, and their spore productions were compared with a soil inoculum. When the SAGE was used as the inoculum on sucrose-agar medium plates the number of spores was increased (35% more than the soil inoculum). After the soil inoculum and SAGE were inoculated on an experimental plant, Zingiber officinale, the percentage root colonization, number of VAM spores, and dry matter content were analyzed. It was observed that the SAGE showed a higher percentage of root colonization (about 10% more), and increases in the number of spores (about 26%) and dry matter (more than 13%) for the two VAM fungal spores than the soil inoculum. The results of this study suggested that the SAGE inoculum may be useful for the mass production of VAM fungi and also for the large scale production of VAM fungal fertilizer.

Factor Analysis of the Cloud Service Adoption Intension of Korean Firms: Applying the TAM and VAM (TAM과 VAM을 적용한 기업의 클라우드 서비스 채택의도의 영향요인 분석)

  • Seo, Kwang-Kyu
    • Journal of Digital Convergence
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    • v.11 no.12
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    • pp.155-160
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    • 2013
  • The global recession circumstances, cloud computing has emerged as a new paradigm in the business IT sector. This paper explores the analysis of cloud service adoption Intension of Korean firms. Especially, we focus on Infrastructure as a Service (IaaS) among cloud services and apply TAM (Technology Acceptance Model) and VAM (Value-based Adoption Model) to analyze cloud service adoption intension The proposed exploratory model tests a number of hypotheses to understand the importance factors of IaaS adoption intension with TAM and VAM included additional cloud service characteristics such as scalability, agility, security, efficiency and reliability. Eventually, the findings of this study can not only help company users gain insights into IaaS adoption, but also help cloud service providers to develop their service effectively and improve marketing strategy in B2B cloud service market.

Aggregate Planning Using Least Cost First Assignment Algorithm of Transportation Problem (운송 문제의 최소비용 우선 배정 알고리즘을 적용한 총괄계획)

  • Lee, Sang-Un
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.21 no.5
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    • pp.181-188
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    • 2021
  • In preparing a aggregate production plan(APP), the transportation method generally uses a linear planning(LP) software package for TSM(transportation simplex method), which seeks initial solutions with either NCM, LCM, or VAM specialized in transportation issues and optimizes them with either SSM or MODI. On the other hand, this paper proposes a transportation method that easily, quickly, and accurately prepares a APP without software package assistance. This algorithm proposed simply assigned to least cost-first, and minimized the inventory periods. Applying the proposed algorithm to 6-benchmarking data, this algorithm can be obtained better optimal solution than VAM or LP for 4 data, and we obtain the same results for the remained 2 data.