Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Use of Sucrose-Agar Globule with Root Exudates for Mass Production of Vesicular Arbuscular Mycorrhizal Fungi

  • Thangaswamy Selvaraj (Centre for Advanced Studies in Botany, University of Madras, and Department of Agricultural Chemistry, and Research Institute of Agricultural Sciences, Sunchon National University) ;
  • Kim, Hoon (Department of Agricultural Chemistry, and Research Institute of Agricultural Sciences, Sunchon National University)
  • Published : 2004.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

A sucrose-agar globule (SAG) was newly introduced to increase production of the vesicular arbuscular mycorrhizal (VAM) fungal spores, Gigaspora gigantea and Glomus fasciculatum. An SAG inoculum and a sucrose-agar globule with root exudates (SAGE) inoculum were prepared, and their spore productions were compared with a soil inoculum. When the SAGE was used as the inoculum on sucrose-agar medium plates the number of spores was increased (35% more than the soil inoculum). After the soil inoculum and SAGE were inoculated on an experimental plant, Zingiber officinale, the percentage root colonization, number of VAM spores, and dry matter content were analyzed. It was observed that the SAGE showed a higher percentage of root colonization (about 10% more), and increases in the number of spores (about 26%) and dry matter (more than 13%) for the two VAM fungal spores than the soil inoculum. The results of this study suggested that the SAGE inoculum may be useful for the mass production of VAM fungi and also for the large scale production of VAM fungal fertilizer.

Keywords

References

  1. New Phytol v.82 Interaction between VA mycorrhiza and Rhizobium and their effect on soyabean in field Bagyaraj,D.J.;A.Manjunath;I.R.B.Patil https://doi.org/10.1111/j.1469-8137.1979.tb07568.x
  2. Fourth European Symposium on Mycorrhizas Becard,G.
  3. Methods in microbiology v.24 no.89 Becard,G.;Y.Piche;J.R.Norris;D.J.Read;A.K.Varma(eds.)
  4. Hort.Sci v.11 Response of apple seedlings to zinc fertilization and mycorrhizal inoculation Benson,N.R.;R.P.Covery
  5. VA mycorrhiza Physiology of VA mycorrhizal association Cooper,K.M.;Powell,C.L.;D.J.Bagyaraj(eds.)
  6. Mycologia v.68 Effects of nitrogen and soil extracts on germination of Glomus mosseae spores Danials,B.A.;S.O.Graham https://doi.org/10.2307/3758901
  7. Soil Biol.Biochem v.18 Vesicular-arbuscular mycorrhizae decrease zine-toxicity to grasses growing in zinc polluted soil Dueck,Th.A.;P.Visser,W.H.O.Emst;H.Schat https://doi.org/10.1016/0038-0717(86)90070-2
  8. Trans. Brit. Mycol. Soc. v.46 Spores of amycorrhizal Endogone species extracted by wet sieving and decanting Gerdemann,J.W.;T.H.Nicolson https://doi.org/10.1016/S0007-1536(63)80079-0
  9. Mycologia Memoir no.5 The Endogonaceae in the Pacific Northwest Gerdemann,J.W.;J.M.Trappe
  10. Ph.D.Thesis,University of London, The influence of a vesicular-arbuscular fungus on the host plant uptake of heavy metals Gildo,A.
  11. Fourth European Symposium on Mycorrhizas Gulielmino,N.;V.Bianciotto,I.Mortini,G.Lingua;P.Bonafante;J.M.Barea(ed.)
  12. VA mycorrhiza Hepper,C.M.;C.L.Powell;D.J.Bagyarj(eds.)
  13. Mycorrhiza-structure, functions, molecular biology and biotechnology Khan,A.G.;M.Belik.;A.Varma;B.Hock(eds.)
  14. Plant Physiol v.127 Transcriptional Responses in the Hemiparasitic plant Triphysaria versicolor to host plant signals Matvienko.M.;M.J.Torres;J.L.Yoder https://doi.org/10.1104/pp.127.1.272
  15. Indian Phytopathol v.52 Growth responses in Acacia nilotica inoculated with VAM fungi(Glomus mosseae),Rhizobium sp. and Trichoerma harzianum PriyaRani;A.Agarwal;R.S.Mehrotra
  16. Techiques in Mycorrhizal research, Raman,N.;V.Mohankumar
  17. Science v.172 Mycorrhizal enhancement of water transport in soybean Safir,G.R.;J.S.Boyer;J.W.Gerdemann https://doi.org/10.1126/science.172.3983.581
  18. Indian J.Experimnet.Biol v.36 Trends in endomycorrhizal research Sahay,N.S.;Sudha;ArchanaSingh;A.Vama
  19. Manual for the identification of VA mycorrhizal fungi Schenck,N.C.;Y.Perez
  20. Soil. Sci. Soc. Anner. J. v.43 Comparison of methods to extract spores of vesicular arbuscular mycorrhizal fungi Smith,W.;H.C.Skipper https://doi.org/10.2136/sssaj1979.03615995004300040019x
  21. Plant Soil v.76 The role of microorganisms in mediation and facilitation the uptake of plant nutrients from soil Tinker,P.B. https://doi.org/10.1007/BF02205569
  22. Avances in food producing systems for arid and semi arid range lands Mycorrhizae and productivity of arid and semi arid range lands Trappe,J.M.;J.T.Manassah;E.J.Brishey(eds.)