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Study on the Proteolytic Activities of Pathogenic Vibrio sp. (비브리오 속의 단백질 분해능에 관한 연구)

  • 양지영;한종흔;이재우;김수광;차재호
    • Journal of Food Hygiene and Safety
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    • v.15 no.2
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    • pp.173-175
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    • 2000
  • Nine strains of pathogenic Vibrio sp. of clinical and environmental origin were examined for the degradation of gelatin, casein and hemolysin which is important to the virulence of this bacterium. Culture filtrates of all nine strains of Vibrio exhibited proteolytic activities. Especially, four strains of V. parahaemolyticus and one V. alginolyticus showed strong gelatin-degrading activity. In terms of hemolytic activity, three V. parahaemolyticus and V. mimicus showed strong $\beta$-hemolysis whereas those of strains of V. alginolyticus, V. fluvialis, V. vulnificus examined lacked this activity.

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Comparison of Performance of Medical Image Semantic Segmentation Model in ATLASV2.0 Data (ATLAS V2.0 데이터에서 의료영상 분할 모델 성능 비교)

  • So Yeon Woo;Yeong Hyeon Gu;Seong Joon Yoo
    • Journal of Broadcast Engineering
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    • v.28 no.3
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    • pp.267-274
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    • 2023
  • There is a problem that the size of the dataset is insufficient due to the limitation of the collection of the medical image public data, so there is a possibility that the existing studies are overfitted to the public dataset. In this paper, we compare the performance of eight (Unet, X-Net, HarDNet, SegNet, PSPNet, SwinUnet, 3D-ResU-Net, UNETR) medical image semantic segmentation models to revalidate the superiority of existing models. Anatomical Tracings of Lesions After Stroke (ATLAS) V1.2, a public dataset for stroke diagnosis, is used to compare the performance of the models and the performance of the models in ATLAS V2.0. Experimental results show that most models have similar performance in V1.2 and V2.0, but X-net and 3D-ResU-Net have higher performance in V1.2 datasets. These results can be interpreted that the models may be overfitted to V1.2.

CLASSIFICATION OF THE EQUIVARIANT LINE BUNDLES OVER $S^1$ (원 위에서의 EQUIVARIANT LINE BUNDLE 의 분류)

  • Kim, Seong-Suk
    • The Journal of Natural Sciences
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    • v.5 no.1
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    • pp.1-3
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    • 1992
  • G가 compact Lie 군이고 $\pi$ : $E to S^1$$S^1$ 상의 G-line bundle 일때, 군 작용이 없다면, 부드러운 trivial G-line bundle $E to S^1$ 은 S(V) $\times$ $\delta to S(V)$ 와 동치이고 부드러운 nontrivial G-line bundle $E to S^1$ 은 S(V) $\times$$z_2$ $\delta to S(V)$/$Z_2$=P(V)와 동치 이다.

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TaqMan probe real-time PCR for quantitative detection of bovine adenovirus type 1 during the manufacture of biologics and medical devices using bovine-derived raw materials (소유래 성분 원재료 사용 생물의약품과 의료기기 제조 공정에서 bovine adenovirus type 1 정량 검출을 위한 TaqMan probe real-time PCR)

  • Ko, Woon Young;Noh, Na Gyeong;Kim, In Seop
    • Korean Journal of Microbiology
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    • v.51 no.3
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    • pp.199-208
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    • 2015
  • Biologics and medical devices manufactured with bovine-derived raw materials have the risk of viral contamination. Therefore, viral validation study is essential to ensure the safety of the products. Bovine adenovirus type-1 (BAdV-1) is one of the common bovine viral pathogens. For quantitative detection of BAdV-1 during the manufacture of biologics and medical devices, a TaqMan probe real-time PCR method was developed. Specific primers and TaqMan probe for amplifying and detecting BAdV-1 DNA were designed. Specificity, limit of detection (LOD), and robustness of the method was validated according to international guideline on the validation of nucleic acid amplification tests for the pathogen detection. The sensitivity of the assay was found to be $7.44{\times}10^1\;TCID_{50}/ml$. The real-time PCR method was reproducible, very specific to BAdV-1, and robust. Moreover, the method was successfully applied to the validation of Chinese Hamster Ovary (CHO)-K1 cells artificially infected with BAdV-1, a commercial CHO master bank, and bovine type 1 collagen. The overall results indicate that this rapid, specific, sensitive, and robust assay can be reliably used for quantitative detection of BAdV-1 contamination during the manufacture of biologics and medical devices using bovine-derived raw materials.

Hydrogen Isotope Effects in Hydrogen Storage Alloy for Separation and Concentration of Hydrogen Isotopes (수소 동위체의 분리농축을 위한 수소저장합금의 수소 동위체 효과)

  • Cho, Sung-Wook
    • Transactions of the Korean hydrogen and new energy society
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    • v.14 no.4
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    • pp.327-334
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    • 2003
  • 경수소와 중수소를 사용하여 Ti1.0Mn0.9V1.1합금의 경우 313K와 353 K에서, $Ti_{1.0}Cr1.5V_{1.1}$합금의 경우 313 K와 338K에서 각각 수소 동위체 효과를 조사하였다. 합금의 결정구조, 각 상의 존재량, 격자상수 등은 Rietveld method에 의해 결정되었다. 두 합금 모두 용도에 관계 없이 중수소의 흡장량이 경수소에 비하여 많았고, 이들 합금의 수소 동위체 효과는 LaNis 합금에 비하여 대단히 크게 나타났다. 실험 온도 범위에서 $Ti_{1.0}Mn_{0.9}V_{1.1}$합금의 경수소화물은 중수소화물에 비하여 안정하였고, Ti1.0Cr1.5V1.7합금에 있어서는 중수소화물이 더욱 안정하였다. 또한 $Ti_{1.0}Cr_{1.5}V_{1.7}$합금이 $Ti_{1.0}Mn_{0.9}V_{1.1}$합금보다 많은 량의 경수소와 중수소를 흡장하였다.

The Study on the Separation of the Subsidiary Elements in Iron and Steel by Using Ion Exchangers (II). The Separation of Anions (이온 교환수지에 의한 철 및 강의 분석에 관한 연구 (제2보). 음이온 성분의 분리)

  • Byoung-Cho Lee;Myon-Yong Park;Kee-Chae Park
    • Journal of the Korean Chemical Society
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    • v.17 no.6
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    • pp.428-433
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    • 1973
  • The quantitative separations of a mixture containing equal amount of each anion such as Si(IV), As(V), P(V), S(VI), W(VI) and Cr(VI) are carried out by the elution through 20${\times}3.14cm^2$ column of anion exchange resin, Dowex 1${\times}$8. The eluents are a mixture of 0.07 M hydrochloric acid and 0.03 M sodium chloride (pH = 1.30) for Si(IV), As(V) and P(V) species, a mixture of 0.6 M sodium chloride and 0.3 M sodium hydroxide for S(VI), W(VI) and Cr(VI) species, and 0.1 N sodium sulfite (pH = 3.48) for P(V) and As(V) species. The subsidiary anions in a standard mixture such as Si(IV), As(V), S(VI), P(V) and W(VI) are separated together from large amount of Fe(III) by the elution through 30cm${\times}3.14cm^2$ column of the resin, Dowex${\times}$50w${\times}$12, using a mixture of 0.1 M sodium nitrate and 2 percent dimethylsulfoxide aqueous solution as an eluent. Si(IV), As(V), S(VI), P(V) and W(VI) eluted together are separated quantitatively under the same conditions as in the separations of the anion mixture. By the conditions obtained in the separations of the standard mixture, Fe(III) and all of the subsidiary anions in steel are quantitatively separated.

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Effects of Vinegar and Lactic Acid on the Survival of Pathogens Causing Food Poisoning of Sliced Raw Fish Meat (식초와 젖산이 생선회 식중독 유래 병원성 세균의 생존에 미치는 영향)

  • 김영만;김경희
    • Journal of Life Science
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    • v.12 no.6
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    • pp.669-675
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    • 2002
  • Introduction of sliced raw fish meat(SRFM) to fast food business has been considered seriously. However bacteria causing food poisoning should be controlled. Organic acids such as vinegar and lactic acid used in the sauce for SRFM were evaluated for their antibacterial activities. At low concentration levels of vinegar and lactic acid exerted strong antibacterial activities toward Vibriu sp.. In contrast, in case of Salmonella typhimurium and Escherichia coli O157:H7 low anitbacterial activities were observed even at relatively high concentrations. Minimum inhibitory concentrations(MIC) of vinegar for V. vulnificus, V. cholerae non-O1, V. parahaemolyticus, V. mimicus, S. typhimurium and E. coli O157:H7 were 16, 18, 16, 12, 26, and $20{\mu}\ell /m\ell, respertively. MIC of lactic acid for V. vilnificus, V. cholerae non-O1, V. parahaemolyticus, V. mimicus, S. typhimurium and E. coli O157:H7 were 20, 25, 25, 25, 40, and $35{\mu}\ell /m\ell, respectively. In case of vinegar bactericidal concentration upon 10 second contact for V. vulnificus, V. cholerae non-O1, V. parahaenolyticus, V. mimicus and E. coli O157:H7 were 8, 14, 10, 4, and 48%, respectively; however, even at 50% colony of S. typhimurium was observed. In case of lactic acid any colony was observed for V. vulnificus, V. cholerae non-O1, V. parahaemolyticus, V. mimicus, S. typhimurium and E. coli O157:H7 at the concentration of 2, 3, 4, 3, 14, and 17%, respectively. Vinegar and lactic acid of low concentration inhibited the growth of Vibrio sp., food poisoning pathogen in SRFM; in contrast, at high concentration these organic acids inhibited Salmonella sp. and Escherichia sp., food poisoning pathogen in other than SRFM.

Effect of Mixture Ratio of Biochar and Peatmoss on the Growth of Aster spathulifolius (바이오차와 피트모스의 혼합비율이 해국 묘 생육에 미치는 영향)

  • Kim, S.J.;Kim, S.J.;Han, S.K.;Kwon, Y.K.;Kwon, Y.H.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.20 no.2
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    • pp.31-38
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    • 2018
  • This study was conducted to investigate the possibility of biochar as an alternative medium to peatmoss using for Aster spathulifolius. We cultivated A. spathulifolius in four potting media with different mixing rates (v/v) of peatmoss (P) and biochar (B) as follows: B0+P3, B1+P2, B2+P1, and B3+P0 with vermiculite 3 + perlite 3. Also, we analyzed the chemical properties of media and the plant growth characteristics. The results were as follows: In case of media's chemical condition, B0+P3 and B1+P2 treatments showed higher tendency (p < 0.05). Plant height on B0+P3 and B1+P2 treatments was much higher than that on other treatments (p < 0.05). Root length on B1+P2 treatment was higher than on B0+P3 treatment (p < 0.05). B0+P3 and B1+P2 treatments showed higher number of leaves and dry biomass than other treatments. Therefore, our results support that Biochar : Peatmoss : Vermiculite : Perlite (1/3 : 2/3 : 1 : 1, v/v) could be a more economical potting medium for A. spathulifolius than peatmoss : vermiculite : perlite (1 : 1 : 1, v/v).