• 한국수산과학회지
• /
• 제36권1호
• /
• pp.24-29
• /
• 2003

규조류 C. calcitrans와 S. costatum, 그리고 Chaetoceros spp. 생육저해 활성을 가지는 해양세균, Shewanella sp. SR-14와 그 저해활성이 없는 V. alginolyticus의 지방산 조성을 분석하고 이들 해양세균과 규조류를 각각 혼합배양하였을 때 규조류의 지방산 조성변화를 측정하였다. Peptone broth에서 $20^{\circ}C$, 3일간 배양한 Shewanella sp. SR-14의 주요 지방산 조성은 16:1n-7 $(29.4\%)$과 16:0 $(19.2\%)$이었으나, V. alginolyticus의 경우는 16:0 $(23.7\%)$ 16:1n-7 $(27.7\%)$ 및 18:1n-7 $(21.0\%)$이었으며, 이들 세균을 Conwy 배지에서 재배양 하였을 때 그 조성은 peptone broth에서 배양한 균체와 약간의 변화가 있었다. 순수배양한 미세조류의 주요 지방산은 C. calcitrans의 경우 16:1n-7 $(33.3\%),$ 16:0 $(27.1\%)$ 및 14:0 $(12.1\%)$ 이었고, S. costatum은 16:1n-7 $(28.9\%),$ 16:0 $(21.6\%)$ 및 20:5 $(19.8\%)$이었다. 세균과 혼합배양한 규조류의 지방산 조성은 Shewanella sp. SR-l4와 혼합배양한 경우 순수배양한 조체에 비하여 포화지방산의 비율은 감소하였으나 불포화지방산의 비율은 증가하였으며, 그 변동율은 C. calcitrans의 경우가 S. costatum보다 높았다. 그러나 V. alginolyticus와 혼합배양한 경우 C. calcitrans는 포화지방산이 증가한 반면 불포화지방산은 감소하는 경향을 나타내었고, S. costatum은 순수 배양한 조체와 큰 차이를 보이지 않았다. 그래서 C. calcitrans는 Shewanella sp. SR-14에 의하여 불포화지방산의 조성이 증가되고, 이러한 지방산은 이 균이 생성하는 조류증식 저해물질과 상승적으로 작용하여 더욱 조류의 증식이 억제되는 것으로 시사되었다.

• 한국패류학회지
• /
• 제15권1호
• /
• pp.49-55
• /
• 1999

양식장에서 폐사하였거나 실활 상태의 소라를 채집하여 호염성 세균을 분리하고 이들 세균중 건강한 소라에 병원성을 나타내는 두 종의 세균에 대하여 형태, 생리적 시험을 행한 결과 Vibrio alginolyticus와 Vibrio anguillarum biovar I으로 동정되었다. 이들 두 종의 세균은 공히 25-3$0^{\circ}C$ 부근에서 가장 잘 생육하였고, 염분농도는 해수보다 약간 낮은 3%내외가 최적 발육농도였다. 이들 두 종의 세균에 심하게 감염된 소라는 아가미의 괴사가 일어나는 것으로 밝혀졌다. 두 종 세균의 급작스런 증식과 소라자체의 환경변화에 따른 활력의 상실 등 복합적인 제반 요인이 동시에 작용될 때 발병이 유발되는 것으로 추정된다.

• 미생물학회지
• /
• 제29권5호
• /
• pp.319-328
• /
• 1991

this study was performed for the purpose of surveying the distribution and characteristics of the Vibrio species in the diseased farm flounders (Paralichthys olivaceus) during the winter season in Korea. Samples were collected along the southern coast in the Sinam, Keoje Island, and Teosu areas from November '90 to Feburary '91. Nine species of Vibrio were identified as V. tubiashii (85 strains), V. damsela (78 strains), V. anguillarum (21 strains), V. compbelli (9 strains), V. fluvialis (9 strain), V. costicola (1 strain), V. alginolyticus (1 strain), V. gazogenes (1 strain), V. marinus (1 strain), and unidentified 3 groups of bacteria (154 strains) which may have related with V. tubiashii were isolated. Eleven kinds of discs (BBL Co.) were used for antibiotic susceptibility test and 116 strains were selected for the experiment. Most bacteria were powerfully inhibited their growth by 4 antibiotics such as cephalothin, chloramphenicol, colistin, and tetracycline, but they were poorly inhibited by 4 antibiotics such as carbenicillin, kanamycin, penicillin G, and streptomycin.

• Journal of Microbiology and Biotechnology
• /
• 제27권3호
• /
• pp.460-470
• /
• 2017

Mussels are major fouling organisms causing serious technical and economic problems. In this study, antifouling activity towards mussel was found in three compounds isolated from a marine bacterium associated with the sea anemone Haliplanella sp. This bacterial strain, called PE2, was identified as Vibrio alginolyticus using morphology, biochemical tests, and phylogenetic analysis based on sequences of 16S rRNA and four housekeeping genes (rpoD, gyrB, rctB, and toxR). Three small-molecule compounds (indole, 3-formylindole, and cyclo (Pro-Leu)) were purified from the ethyl acetate extract of V. alginolyticus PE2 using column chromatography techniques. They all significantly inhibited byssal thread production of the green mussel Perna viridis, with $EC_{50}$ values of $24.45{\mu}g/ml$ for indole, $50.07{\mu}g/ml$ for 3-formylindole, and $49.24{\mu}g/ml$ for cyclo (Pro-Leu). Previous research on the antifouling activity of metabolites from marine bacteria towards mussels is scarce. Indole, 3-formylindole and cyclo (Pro-Leu) also exhibited antifouling activity against settlement of the barnacle Balanus albicostatus ($EC_{50}$ values of 8.84, 0.43, and $11.35{\mu}g/ml$, respectively) and the marine bacterium Pseudomonas sp. ($EC_{50}$ values of 42.68, 69.68, and $39.05{\mu}g/ml$, respectively). These results suggested that the three compounds are potentially useful for environmentally friendly mussel control and/or the development of new antifouling additives that are effective against several biofoulers.

• 한국식품영양과학회지
• /
• 제30권2호
• /
• pp.222-227
• /
• 2001

The members of the genus Vibrio include harmless aquatic strain as well as strains capable of causing infections in human and fish. Pathogenic mechanisms are only understood for Vibrio cholerae O1 and O139 and not for the majority of Vibrio species. Twelve clinical and nonclinical strains were examined by in vitro and in vivo experiments for the importance of extracellular enzymes as a virulence determinant of Vibrio species. In vivo cytotoxicity assay was performed by injecting approximately $10^{8}$ cells/mL into mice (BALB/c). V. harvyi and V. vulnificus showed 100% lethality within 3hr after bacterial injection. V. fluvialis and four strains of V. parahaemolyticus showed 50% lethality within 4hr. V. mimicus, V. alginolyticus and V. furnissii revealed 30% lethality within 9hr. Nonclinical strains, V. campbellii and V. ordalii, did not show any lethality. In vitro protease and hemolytic activities were also good indicators for clinical and nonclinical strains of Vibrio species. The clinical strains showed much higher activities than nonclinical strains. The activity of some clinical strains of re-isolates was evidently increased. Most clinical strains had $\beta$ hemolytic activity. The results demonstrate that the prevalent distribution of extracellular proteases in pathogenic Vibrio sp. implies their importance as a virulence determinant.

• 대한임상검사과학회지
• /
• 제36권1호
• /
• pp.1-6
• /
• 2004

The enterobacterial repetitive intergenic consensus (ERIC)-PCR is a recently described DNA fingerprinting technique based on amplification of repetitive element distributed in bacteria. We applied of ERIC-PCR to clinical isolates of Vibrio parahaemolyticus and other bacteria associated diarrhea. Twenty isolates of V. parahaemolyticus were used for intragenic genotyping, which were isolated from 2001 to 2002 in Chungbuk National University hospital. For interspecies genotyping, V. vulnificus, V. alginolyticus, V. parahaemolyticus, Escherichia coli, Salmonella and Shigella spp. were used. The genotyping were analyzed by ERIC-PCR. The genotyping of V. parahaemolyticus were grouped two major pattern (A, B) and were subdivided into 10 subtypes (A1, A2, B1-B8) by ERIC-PCR. These method distinctly differentiated bacterial species associated diarrhea. Those results show that ERIC-PCR can be reliable and efficient method for genotyping of V. parahaemolyticus and bacteria associated diarrhea.

• Journal of Microbiology
• /
• 제41권4호
• /
• pp.312-319
• /
• 2003

In an attempt to develop a method for rapid and accurate identification of six Vibrio species that are clinically important and most frequently detected in Korea, 16S rDNA restriction fragment length polymorphism (RFLP) of Vibrio type strains, as well as environmental isolates obtained from the Korean coastal area, was analyzed using ten restriction endonucleases. Digestion of the 16S rDNA fragments amplified by polymerase chain reaction (PCR) with the enzymes gave rise to 2~6 restriction patterns for each digestion for 47 Vibrio strains and isolates. An additional 2~3 restriction patterns were observed for five reference species, including Escherichia coli, Aeromonas hydrophila, A. salmonicida, Photobacterium phosphoreum, and Plesiomonas shigelloides. A genetic distance tree based on RFLP of the bacterial species correlated well with that based on 16S rDNA sequences. The very small 16S rDNA sequence difference (0.1%) between V. alginolyticus and V. parahaemolyticus was resolved clearly by RFLP with a genetic distance of more than 2%. RFLP variation within a species was also detected in the cases of V. parahaemolyticus, V. proteolyticus, and V. vulnificus. According to the RFLP analysis, six Vibrio and five reference species were assigned to 12 genotypes. Using three restriction endonucleases to analyze RFLP proved sufficient to identify the six pathogenic Vibrio species.

• 한국수산과학회지
• /
• 제38권6호
• /
• pp.359-364
• /
• 2005

The distributions of sanitary indicative bacteria and pathogenic bacteria in seawater and four species of farmed fishes, including oliver flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagrus major) and sea bass (Lateolabrax japonicus), collected at fish farms located in the southern coastal area of Korea were investigated from May to October in 2004. The detection rates of fecal coliform and Entirococcus spp. of sanitary indicative bacteria in all samples were $38.9\%$ and $23.8\%$, respectively. The occurrence of fecal coliform was highest of $58.3\%$ in Busan, Geoje and Wando area, followed Yeosu $33.3\%$, Jeju $12.5\%$, Tongyeong $11.1\%$. The occurrence of Enterococcus spp. was highest In Wando area ($45.8\%$), followed by Yeosu ($33.3\%$), Tongyeong ($22.2\%$), Busan ($16.7\%$), Geoje and Jeju ($12.5\%$). The detection rate of fecal coliform was higher than that of Enterococcus spp., except in the Tongyeong area. There was no difference in the detection rate of fecal coliform from May to October, but the detection rate of Enterococcus spp. increased with seasonal warming seawater temperature. Among the pathogenic bacteria, the detection rate of Vibrio alginolyticus ($49.2\%$) in all samples was highest, followed by V. parahaemolyticus ($36.5\%$), Staphylococcus aureus ($6.3\%$), Salmonella sp. ($2.4\%$). However, V cholerae, V. vulnificus and Shigella sp. were not detected in all tested samples. The detection rates of V. parahaemolyticus and V. alginolyticus increased with seasonal warming seawater temperature from May to August.

• 한국수산과학회지
• /
• 제35권4호
• /
• pp.451-453
• /
• 2002

The rotation of the flagellar motor is powered by the electrochemical gradient of specific ions across the cytoplasmic membrane. Recently, the gents of the Na'-driven motor have been cloned from marine bacterium of Vibrio sp. and some of the motor proteins have been purified and characterized. Also, motx gene encoding a channel component of the sodium type flagellar motor was identified from Vibrio Huuiaiis (KTCC 2473). The amino acid sequence of MotX protein from V, Huvialis shared 90, 85, $85\%$ identity with V, cholerae, V. alginolyticus, V parahaemolyticus, respectively. We have studied the localization of the expressed MotX protein in Escherichia coli by immune-gold labeling of ultra-thin frozen section. Our observation of the expressed protein indicated that MotX protein could be existed as attachment to inner membrane in E. coli.

• Fisheries and Aquatic Sciences
• /
• 제7권1호
• /
• pp.10-15
• /
• 2004

A total of 52 pathogenic Vibrio strains was isolated from the Gwangan Beach during summer in 2003. The isolated vibrios were composed of 6 different species: V. parahaemolyticus, V. cholerae non O1, V. fluvialis, V. vulnificus, V. alginolyticus, and V. mimicus. V. parahaemolyticus was most predominant as $46\%$ (24/52), V. cholerae non O1 was the second with $23\%$ (12/52), and V. fluvialis was the third with $17\%$ (9/52). Among the isolated strains, 22 strains showed hemolytic, proteolytic or ureolytic activity. Eight strains showed both hemolysin and protease activities, and either 6 strains showed only hemolysin activities and 7 strains only protease activities. Only one strain of V. parahaemolyticus isolates showed urease activity. The urease-positive V. parahaemolyticus strain (V. parahaemolyticus S25) showed the same biochemical characteristics as the reference strain, V. parahaemolyticus KCTC 2471 (urease­negative) except for urease production. To compare the degree of virulence of Vibrio strains having different pathogenic factors, hemolysin, protease, or urease-positive strains were injected into groups of 10 each of ICR mice (7- to l0-week-old male). The lethal rate of urease-positive V. parahaemolyticus S25 was significantly high, being $70\%$. Protease-positive strains showed $40-60\%$ of lethal rate. Hemolysin-positive strains showed no mortality, similar to non-pathogenic V. parahaemolyticus KCTC 2471 and V. parahaemolyticus FM12.