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http://dx.doi.org/10.5657/kfas.2002.35.4.451

Detection of the Recombinant MotX Protein Vibrio fluvialis in Escherichia coli with Immuno-Gold Labeling Method  

LEE Jong Hee (Department of Biotechnology and Bioengineering, Pukyong National University)
Park Jae Hyun (Department of Biotechnology and Bioengineering, Pukyong National University)
Kim Sun Hoi (Department of Biotechnology and Bioengineering, Pukyong National University)
An Sun Hee (Department of Biotechnology and Bioengineering, Pukyong National University)
Kong In Soo (Department of Biotechnology and Bioengineering, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.35, no.4, 2002 , pp. 451-453 More about this Journal
Abstract
The rotation of the flagellar motor is powered by the electrochemical gradient of specific ions across the cytoplasmic membrane. Recently, the gents of the Na'-driven motor have been cloned from marine bacterium of Vibrio sp. and some of the motor proteins have been purified and characterized. Also, motx gene encoding a channel component of the sodium type flagellar motor was identified from Vibrio Huuiaiis (KTCC 2473). The amino acid sequence of MotX protein from V, Huvialis shared 90, 85, $85\%$ identity with V, cholerae, V. alginolyticus, V parahaemolyticus, respectively. We have studied the localization of the expressed MotX protein in Escherichia coli by immune-gold labeling of ultra-thin frozen section. Our observation of the expressed protein indicated that MotX protein could be existed as attachment to inner membrane in E. coli.
Keywords
Viblio fluvialis; Mod Sodium channel; Immuno-gold labeling;
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