• Microbiology and Biotechnology Letters
• /
• v.41 no.1
• /
• pp.52-59
• /
• 2013

The nutritional requirements for the maximum production of lipopeptides by Bacillus subtilis N7 (B. subtilis N7) were investigated and optimized using response surface methodology (RSM) under shake flask fermentation. A one-factor-at-a-time experimental setup was used to screen carbon and nitrogen sources. A Plackett-Burman design (PBD) was employed to screen the most critical variables for lipopeptides production amongst ten nutritional elements. The central composite experimental design (CCD) was finally adopted to elucidate the composition of the fermentation medium. Statistical analyses (analysis of variance, ANOVA) of the results showed that KCl, $MnSO_4$ and $FeSO_4{\cdot}6H_2O$ were important components and that their interactions were strong. Lipopeptide production was predicted to reach 709.87 mg/L after a 60 h incubation using an optimum fermentation medium composed of glucose 7.5 g/L, peanut oil 1.25 g/L, $MgSO_4$ 0.37 g/L, $KH_2PO_4$ 0.75 g/L, monosodium glutamate 6.75 g/L, yeast extract and $NH_4Cl$ (5:3 w/w) 10 g/L, KCl 0.16 g/L, $FeSO_4{\cdot}6H_2O$ 0.24 mg/L, $MnSO_4$ 0.76 mg/L, and an initial pH of 7.0. Lipopeptide production ($706.57{\pm}3.70$ mg/L) in the optimized medium confirmed the validity of the predicted model.

• Journal of Digital Convergence
• /
• v.14 no.12
• /
• pp.55-63
• /
• 2016

Recently, FTA may go beyond the agreement of the two countries, and also FTA is tinged aspects of Mega FTA between the economic sphere, under the situation that FTA and the business model are mutually intertwined in convergence industrial environments. Since South Korea came into force in 52 countries and 15 cases of FTA, South Korea have completed FTA network with the United States, as well as ASEAN and most of OECD member countries, and South Korea is now a critical time to take advantage of the FTA to determine the South Korea's economic performance. This paper examined the previous studies of FTA utilization and analyzed practical usages and problems of SMEs' FTA utilization. Through analysis, this paper suggests that the Training Plans of SMEs' Origin Management Specialist for the Improvement of FTA Utilization is as follows: to build up the effective management system of origin supports, to enrich the origin management competency, to reinforce the operation of supporting system, and to strengthen practical capacity of Origin administrative staff. Based on the results, it is expected to provide the policy implications for the FTA utilization in the future.

• Journal of Korean society of Dental Hygiene
• /
• v.24 no.1
• /
• pp.9-16
• /
• 2024

Objectives: The demand for dental care is expected to increase as the population ages. This study aimed to predict the utilization of dental implant care following the expansion of national health insurance benefits for dental implants. Methods: Multiple linear regression analysis was performed on HIRA big data open portal data and DNN-based artificial intelligence models to forecast the utilization of dental care in relation to the national health insurance coverage for dental implants. Results: National health insurance coverage of dental implants was found to be associated with the number of patients using dental implant services and demonstrated a statistical significance. The dental implant services utilization increased with the increased dental implant health insurance benefits for the elderly population, increased mean by region, increased number of dental institutions by region, and increased health insurance coverage rate for dental implants. However, the dental implant services utilization decreased with the increased number of older people living alone and increased size of dental institutions. Conclusions: With the expansion of the national health insurance coverage for dental implants, it is predicted that the utilization of dental implant medical services will increase in the future.

• Journal of Korea Technology Innovation Society
• /
• v.17 no.4
• /
• pp.703-732
• /
• 2014

Meta-evaluation for national R&D programs should be established within the framework of whole R&D system linked with science and technology needs and objectives. The R&D program evaluation system can be understood as a circulation of input, implementation, output, and utilization. In the result of the Delphi survey, which consisted of twenty-four experts (performed in three rounds), twenty-four indices were developed. The meta-evaluation model included sub-items which were important points of view of it as follow (eight items): propriety of planning, sufficiency of information, and propriety of evaluators in evaluation input; appropriateness of method and appropriateness of procedure in evaluation implementation; credibility of output in evaluation output; and usefulness of report and application of evaluation utilization. As the application of the meta-evaluation model, the total mean was lower than 3.0 of the median value of 2.73 (5 point Likert scale). Finally, it was suggested that the feedback of evaluation results should be more enforced to R&D program improvement particularly (evaluation utilization 2.50).

• Journal of the Korea Society for Simulation
• /
• v.15 no.4
• /
• pp.143-151
• /
• 2006

This paper deals with the simulation model for design and operations of A-Mart logistic center for low temperature products. In developed simulation model, receiving docks, digital classification system (DCS), shipping docks, material handling devices and manual sorting stations are considered. Five types of cargo such as fruits, palletized fruits, delicatessen and fisheries, vegetables and refrigerated cargoes are considered. The simulation model and process animation are developed using the simulation package ARENA. Among various design and operation alternatives consisting of the number of workers of receiving dock, allocation of receiving docks by cargo types, DCS capacity, the number of folk-lift, the number of manual sorting operators and overall layout, the best alternatives of each subsystem are selected by simulation analysis. The major performance measures such as DCS throughput, utilization of operators at each station, receiving docks utilization and folk-lift utilization are considered for the alternative evaluation.

• Journal of Korean society of Dental Hygiene
• /
• v.15 no.2
• /
• pp.235-243
• /
• 2015

Objectives: The purpose of the study is to investigate the oral health and oral health beliefs in industrial workers and to analyze the influencing factors on dental health care utilization. Methods: The subjects were 280 adults from 16 to 64 years old in Seoul and Gyeonggi from June 20 to July 31, 2014, A self-reported questionnaire was completed after receiving informed consent. The independent variables consisted of predisposing, enabling, and need factors. The predisposing factors included gender, age, residence area, number of family. The enabling variables included monthly income, education, occupation, type of employment. The need factors included subjective oral health recognition and oral health belief model. These three variables had a direct and indirect influence on dental clinic use. The types of occupation were classified into desk duties, merchandizing and service duties technology and others by KSCO-6. Results: The relating factors to dental health care utilization were sex, oral health beliefs perceived benefits, perceived barriers, and self-efficacy. Female tended to have the higher oral health beliefs perceived benefits, perceived barriers(p<0.01), self-efficacy(p<0.05). Conclusions: Those who received frequent oral examination and health instruction tended to have a favorable impact on maintenance of oral health status and improvement in quality of life.

• Health Policy and Management
• /
• v.29 no.2
• /
• pp.160-171
• /
• 2019

Background: This study purposed to compare the difference on medical utilization and health expenditure of baby boomer generation by depression between gender. Methods: Korea Welfare Panel Survey 2016, provided by the Korea Institute for Health and Social Affairs, was used for the analysis. For the research, we used the two-part model, yes or no of use (part 1), and frequency of use (part 2) for medical utilization. The dependent variables are the whether or not to use of hospitalization services, outpatient services, length of stay, outpatient service visits, and health expenditure. And the independent variables are used as the predisposing (education, spouse presence), enabling (insurance type, private insurance, economic activity, income), and need (chronic disease, self-rated health, disability) factors in the Andersen behavior model. Depression was used as intervening variables. Structural equation model and multiple group analysis by gender were used. Results: There were differences in the medical care usage and cost between men and women in baby boomer. For men, mediating effects of depression were present at the hospitalization (yes/no), length of stay, and health expenditure. On the other hand, for women, the mediating effect of depression was found only at the outpatient visits. Specially, depression was working at the medical services by the different way between gender. The size of effect (multiple group analysis) was affected by significant differences between men and women. Conclusion: This study found that the mediating effect of depression is increased medical usage and health expenditure and the effect factors are different by gender. Therefore, it is necessary to establish a medical care policy considering the socio-economic characteristics of baby boomers.

• Health Policy and Management
• /
• v.31 no.1
• /
• pp.125-139
• /
• 2021

Background: In Korea, the health gap widens due to the number of medical resources and access to medical services between metropolitan and rural. The purpose of this study is to identify the impact of residential migration on medical utilization and accessibility. Methods: This study extracted 528,516 claimed cases in the National Health Insurance Service-Cohort Sample Database from 2006 to 2015. Subjects were classified into two groups by the magnitude of the region, the metropolitan and the rural. The inversed probability weights were calculated for each group. And coefficients of the two-part model were estimated by generalized estimation equation. Results: Those who moved region from metropolitan to rural tend to increase the length of stay and inpatients with ambulatory care sensitive conditions (ACSC) disease. Contrariwise, those who moved areas from rural to metropolitan tend to decrease the total medical cost, the adjusted patient days, the number of outpatients and the number of outpatients and inpatients with ACSC disease. Conclusion: This study identified that between the residents who continued to reside in the region and the migrants, there were significant differences in the medical accessibility, quality of primary care, and unmet medical need.

• Journal of Korean Society of Archives and Records Management
• /
• v.23 no.2
• /
• pp.95-115
• /
• 2023

This study designed a data model linked to Wikidata and examined its applicability to increase the utilization of the digital archive records of the National Debt Redemption Movement, registered as World Memory Heritage, and implications were derived by analyzing the existing metadata, thesaurus, and semantic network graph. Through analysis of the original text of the National Debt Redemption Movement records, key data model classes for linking with Wikidata, such as record item, agent, time, place, and event, were derived. In addition, by identifying core properties for linking between classes and applying the designed data model to actual records, the possibility of acquiring abundant related information was confirmed through movement between classes centered on properties. Thus, this study's result showed that Wikidata's strengths could be utilized to increase data usage in local archives where the scale and management of data are relatively small. Therefore, it can be considered for application in a small-scale archive similar to the National Debt Redemption Movement digital archive.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.4
• /
• pp.730-738
• /
• 2016

est19 is a gene from Bacillus sp. K91 that encodes a new esterase. A comparison of the amino acid sequence showed that Est19 has typical Ser-Gly-Asn-His (SGNH) family motifs and could be grouped into the SGNH hydrolase family. The Est19 protein was functionally cloned, and expressed and purified from Escherichia coli BL21(DE3). The enzyme activity was optimal at 60℃ and pH 9.0, and displayed esterase activity towards esters with short-chain acyl esters (C₂-C₆). A structural model of Est19 was constructed using phospholipase A1 from Streptomyces albidoflavus NA297 as a template. The structure showed an α/β-hydrolase fold and indicated the presence of the typical catalytic triad Ser49-Asp227-His230, which were further investigated by site-directed mutagenesis. To the best of our knowledge, Est19 is a new member of the SGNH hydrolase family identified from thermophiles, which may be applicable in the industrial production of semisynthetic β-lactam antibiotics after modification.