• 대한약침학회지
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• 제9권2호
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• pp.105-111
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• 2006

Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. Results : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. The fractions obtained from gel filtration chromatography was analyzed by using SDS-PAGE and propionic acid/urea polyacrylamide gel electrophoresis. The melittin obtained from the gel filtration contained residual amount of phospholipase $A_2$ and a protein with molecular weight of 6,000. The contaminating proteins were removed by the second gel filtration chromatography. Conclusion : Gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis are useful to separate peptide components including melittin from bee venom.

• 한국자원식물학회지
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• 제34권4호
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• pp.287-296
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• 2021

최근 전 세계적으로 지구온난화가 가속되면서 작물의 생산에 극심한 피해가 야기되는바 극한의 고온 스트레스에 따른 참당귀의 생육특성 및 지표성분을 확인하고 효율적인 참당귀의 재배를 위한 기초자료를 제공하고자 하였다. 2018년, 2019년의 기상관측데이터를 활용하여 실험온도(Control, 28℃, 34℃, 40℃)를 설정하였다. 그리고 식물생장상을 통해 실험온도를 처리하여 실험포지에서 이식 및 생장을 거쳐 생육특성 및 유용성분함량을 분석하였다. 실험이 수행되어진 실험포지는 평균 대기온도 19.38℃, 평균 토양온도 21.34℃, 평균대기습도 81.31 %, 평균 토양습도 0.18 m³/m³, 평균 일사량 162.05 W/m²로 관측되었으며, 토양의 이화학적 특성은 사질식양토에 유기물함량 2.66 %, 유효인산 868.52 mg/kg, 전질소 0.14%, pH 6.65, 칼륨, 칼슘, 마그네슘, 나트륨은 각각 0.95, 7.38, 1.46, 0.15 cmol⁺/kg로 분석되었다. 그리고 활착율(85 %)과 전체 높이(38.66 cm), 생중량(41.3 g), 건중량(14.24 g) 등과 같은 대부분의 생육특성은 대조구 대비 28℃ 실험구에서 가장 높은 생육을 보였다. 하지만 유용성분함량의 유의성은 확인되지 않았으나 대조구 대비 34℃ 실험구에서 가장 높은 값을 나타냈다. 이러한 결과는 고온의 스트레스로 식물체 조직의 노화 및 광합성량 감소, 생장지연 등과 같은 생육장애 요인에 의해 실험구별 생육의 차이가 발생했으며, 유용성분의 함량 또한 실험구별 생육장애에 의해 동화산물의 축적이 저조해져 발생된 현상이라 판단된다.

• Asia pacific journal of information systems
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• 제5권2호
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• pp.143-176
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• 1995

We present an overview of the Extensional Object Model (ExOM) and describe in detail the learning and classification components which integrate concepts from machine learning and object-oriented databases. The ExOM emphasizes flexibility in information acquisition, learning, and classification which are useful to support tasks such as diagnosis, planning, design, and database mining. As a vehicle to integrate machine learning and databases, the ExOM supports a broad range of learning and classification methods and integrates the learning and classification components with traditional database functions. To ensure the integrity of ExOM databases, a subsumption testing rule is developed that encompasses categories defined by type expressions as well as concept definitions generated by machine learning algorithms. A prototype of the learning and classification components of the ExOM is implemented in Smalltalk/V Windows.

• Coupled systems mechanics
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• 제9권5호
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• pp.397-410
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• 2020

The present investigation is concerned with two-dimensional deformation in a homogeneous isotropic non local thermoelastic solid with two temperatures due to thermomechanical sources. The theory of memory dependent derivatives has been used for the study. The bounding surface is subjected to concentrated and distributed sources (mechanical and thermal sources). The Laplace and Fourier transforms have been used for obtaining the solution to the problem in the transformed domain. The analytical expressions for displacement components, stress components and conductive temperature are obtained in the transformed domain. For obtaining the results in the physical domain, numerical inversion technique has been applied. Numerical simulated results have been depicted graphically for explaining the effects of nonlocal parameter on the components of displacements, stresses and conductive temperature. Some special cases have also been deduced from the present study. The results obtained in the investigation should be useful for new material designers, researchers and physicists working in the field of nonlocal material sciences.

• 반도체디스플레이기술학회지
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• 제14권2호
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• pp.67-72
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• 2015

For the development of UFS device test system, M-PHY specifications should be matched with MIPI-standard which is analog signal protocol. In this paper, the implementation methodology and hardware structure for the M-PHY AFE (Analog Front End) Block was suggested that it can be implemented using universal components without ASIC process. The testing procedure has a jitter problem so to solve the problems we using ASIC process, normally but the ASIC process needs a lot of developing cost making the UFS device test system. In is paper, the suggestion was verified by the output signal which was compared to the MIPI-standard on the Prototype-board using universal components. The board was reduced the jitter on the condition of HS-TX and 5.824 Gbps Mode in SerDes (Serialize-deserializer). Finally, the suggestion and developed AFE block have a useful better than ASIC process on developing costs of the industrial UFS device test system.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2002년도 추계학술대회 논문집
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• pp.411-415
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• 2002

In this paper, a mechanical probe for CMM (Coordinate Measuring Machine) with a three-axis force-sensing unit is proposed, which is capable of measuring an actual contact position without the lobbing effect and the pre-travel error. The force-sensing unit detects the external force, which is act on the stylus of CMM during the measuring process. Thus, the contact point of the stylus of CMM can be estimated ken the direction of measured force components. Based on the structural analysis of the proposed CMM probe, the transformation matrix is derived and calibrated so that it shows linear relationships between the estimated force components from the output voltages and the real input forces. And, the relationships are verified through the computer simulation. The results show that the proposed mechanical probe is very useful fur detecting the contacting force components on measuring process of CMM.

• 한국축산식품학회지
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• 제21권3호
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• pp.246-255
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• 2001

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of muscles extracted with distilled water, saline solution, SDS or Trition X-100 showed simular protein patterns between Hanwoo and Holstein meat, indicating that SDS-PAGE technique may not be useful for the identification between Hanwoo and Holstein meat. Lectine blot analysis of muscle extracted with distilled water demonstrated that Hanwoo and Holstein meat had similar affinities for concanavalin A (Con A), ricinus communis agglutinin (RCA-120), ulex europaeus agglutinin (UEA-1) or peanut agglutinin (PNA) lectins. However, approximately 32.1 kDa component of Hanwoo meat showed high affinity for dolichos biflorus agglutinin (DBA) lectin. On the contrary, high molecular weight components of Holstein meat had the specific affinity for wheat germ agglutinin (WGA) lectin. Hanwoo meat-specific components were observed by lectin staining of heat-denatured meat at 100$^{\circ}C$ for 30 sec. Also, the component of heat-denatured meat at 100$^{\circ}C$ for 30 sec, which was slightly smaller than Hanwoo meat-specific component, was concentrated specifically in Holstein meat.

• PNF and Movement
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• 제7권1호
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• pp.17-23
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• 2009

Purpose : To provide a convenient framework for PNF practice, we reviewed the relationship between ICF framework and PNF framework. Methods : We reviewed literatures related with ICF and PNF. Results : ICF model is useful tool for physical therapist who is working in PNF to identify the interactions the components of individual's health, especially the relationship between functioning and disability. A framework for PNF is philosophy which included the concept, functional approach. It is essential to identify primary activity limitation and causal impairment in PNF field and evaluate the their relationship. The ICF model can be used to classify the examination information. Next step is to prioritize the activity limitation and then evaluate the interrelationships among each components of the ICF framework. Conclusions : ICF model guides physical therapist in PNF practice to identify patient problems and evaluate the interrelationship of components of their health. This model is logical framework to directs functional approach as PNF philosophy to be approached the goal.

• Journal of Microbiology
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• 제35권3호
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• pp.193-199
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• 1997

From several industrial wastewaters, 14 bacterial strains which degrade benzene, toluene, o-xylene, m-xylene, or p-xylene (BTX) were obtained. These strains were characterized as to their species composition and the substrate range, kinetic parameters and the substrate interactions were investigated. Although BTX components have a similar chemical structure, isolated strains showed different substrate ranges and kinetic parameters. None of the strains could degrade all of BTX components and most of them showed an inhibition (Haldane) kinetics on BTX, BTX mixtures were removed under inhibitory substrate interactions with variation in the intensity of inhibition. For a complete degradation of BTX, a defined mixed culture containing three different types of patyways was constructed and all of the BTX components were simultaneously degraded with the totla removal rate of 225.69 mg/g biomass/h Judging from the results, the obtained mixed culture seems to be useful for the treatment of BTX-contaminated wastewater or groundwater as well as for the removal of BTX from the contaminated air stream.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2005년도 추계학술대회논문집
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• pp.127-130
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• 2005

There are many methods in feature extraction have been developed. Recently, principal components analysis (PCA) and independent components analysis (ICA) is introduced for doing feature extraction. PCA and ICA linearly transform the original input into new uncorrelated and independent features space respectively In this paper, the feasibility of using nonlinear feature extraction will be studied. This method will employ the PCA and ICA procedure and adopt the kernel trick to nonlinearly map the data into a feature space. The goal of this study is to seek effectively useful feature for faults classification.