• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.11 no.1
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• pp.34-41
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• 2001

Passive samplers have been used for personal, indoor, and outdoor air monitoring of VOCs at ppb concentrations in community and office environments. The path length of modified passive sampler was shortened, so it was intended to increase an uptake rate. The performance of the modified 3M 3500 organic vapor monitor(OVM) as a tool for assessing exposures to toxic air pollutants in nonoccupational community environments was evaluated using combined controlled test atmospheres of six selected target volatile organic compounds(VOCs): benzene, methyl tert-butyl ether(MTBE), chloroform, 1,4-dichlorobenzene, tetrachloroethylene, and toluene. The experiments were conducted by exposing the dosimeters to concentrations of $50{\sim}100{\mu}g/m^3$ on six face velocity(0.00, 0.02, 0.06, 0.12, 0.20, 0.30 m/sec) for 24 hours. If the uptake rate was increased, that means that we could use the passive sampler more effectively. The uptake rates were increased linearly according to reduce the path length. Although the diffusion path length was shortened, the change of uptake rate was within ${\pm}25%$ of theoretical value, indicating that the modified passive sampler(TM) can be effectively used over the range of concentrations and environmental conditions tested with a 24-h sampling period if the face velocities were over 0.12 m/s for 6 components of VOCs. But when the face velocities were less than 0.12 m/s, uptake rates were reduced more than expected values. So, the passive sampler with the shortened path length should be used at indoor or outdoor environment where the face velocity should be over about 0.10 m/s. If the path length was shortened more, the uptake rate was more effected by starvation.

• The Korean Journal of Nuclear Medicine
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• v.8 no.1_2
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• pp.29-37
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• 1974

The $^{99m}Tc$-pertechnetate thyroid uptake rates(20 min) were measured in 24 healthy normal subjects, 140 patients with nontoxic goiter and 98 patients with thyrotoxicosis who were treated at the Thyroid Clinic, Seoul National University Hospital, from August 1972 to August 1973. Diagnostic reliabilities and correlations between $^{99m}TcO_4$-thyroid uptake rate (20 min) and other thyroid function tests were evaluated. The observed results were as follows 1. The $^{99m}TcO_4$-thyroid uptake rates (20 min) in normal subjects, euthyroid group and hyperthyroid group were $4.1{\pm}0.9%,\;5.2{\pm}1.8%\;and\;29.7{\pm}10.6%$. There was a significant difference between the mean of the euthyroid group and the mean of the hyperthyroid group and so differentiation between them can be easy. 2. In the diagnosis of hyperthyroidism, the reliabilities of $^{99m}TcO_4$- thyroid uptake rate(20 min), $^{131}I$ thyroid uptake rate(24hrs), serum $T_3$ resin uptake rate, serum $T_4\;and\;T_7\;were\;87.9{\sim}97.9%,\;81.2{\sim}94.4%,\;87.9{\sim}97.9%,\;90.5{\sim}99.3%\;and\;93.7{\sim}100%$. $^{99m}TcO_4$-thyroid uptake rate(20 min) is more accurate than $^{131}I$ thyroid uptake rate (24 hrs) in the diagnosis of hyperthyroidism. 3. $^{99m}TcO_4$-thyroid uptake rate (20 min) was well correlated with $^{131}I$ thyroid uptake rate (24 hrs), serum $T_3$ resin uptake rate, serum $T_4\;and\;T_7$. Points in favor of $^{99m}Tc$ are that it gives a small radiation dose to the thyroid, that tests can be repeated at the short interval, the study can be completed at a single patient visit and it is particularly well suited for the assessment of thyroid function in patients being treated with an antithyroid drug.

• The Korean Journal of Ecology
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• v.26 no.4
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• pp.165-172
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• 2003

The objectives of this study were to examine the physiological responses to ozone and to measure ozone uptake rates of Betula species exposed to relatively high concentration of pollutants. At the end of the growing season, photosynthesis, pigments contents, antioxidants (SOD and GR) and ozone uptake rates were measured or estimated at the leaves of five Betula species (Betula costata, B. davurica, B. platyphylla var. japonica, B. schmidtii and B. ermanii) exposed to 100ppb ozone concentration. On the termination of the experiment, growth effects were determined by measuring leaf area and dry weights of leaf, stem and root. Ozone treatment showed the significant reduction the leaf area and dry weight of four Betula species, except for B. ermanii. Shoot / root (SR) ratio of five species represented two different types. SR ratio of B. costata and B. davurica were lower than control, in contrast, SR ratio of B. platyphylla var. japonica, B. schimidtii and B. emani, were higher than that of control. The photosynthetic responses of five species were different in responses to ozone exposure. Four species, except for B. emanii, maintained or increased the stomatal conductance, but B. emanii decreased both stomatal conductance and photosynthesis. SOD activities of five species decreased by the ozone exposure, especially B. ermanii showed the largest reduction, GR activities of B. platyphylla var. japonica and B. schmidtii increased, B. costata and B. emanii decreased. Instantaneous ozone uptake rate was the highest at the leaves of B. ermanii and B. costata, ozone uptake per seedling was the highest at the leaf of B. schmidtii and B. emanii. It was concluded that B. costata, B. davurica and B. platyphylla var. japonica, appeared the growth reduction and visible ozone injury, were sensitive species to ozone, and B. schmidtii with the increased antioxidant activity and B. ermanii without the growth reduction were relatively resistant species to high ozone concentration at the early growing stage.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.16 no.4
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• pp.196-205
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• 2011

To investigate the contribution of macroalgae to biogeochemical nutrients and carbon cycles, we measured the uptake rates of nutrients and $CO_2$ and characteristics of fluorescence of Saccharina japonica (Laminaria japonica Areschoug) using an incubation method in an acrylic chamber. From January to May 2011, S.japonica was sampled at Ilkwang, one of well-known macroalgae culture sites around Korea and ranged 46~288 cm long and 4.8~22.0 cm wide of whole thallus. The production rate of dissolved oxygen by S. japonica (n=25) was about $6.9{\pm}5.8{\mu}mol\;g^{-1}$ fresh weight(FW) $h^{-1}$. The uptake rate of total dissolved inorganic carbon ($TCO_2$), calculated by total alkalinity and pH, was $8.9{\pm}7.9{\mu}mol\;g^{-1}\;FW\;h^{-1}$. Mean nutrients uptake were $175.6{\pm}161.1\;nmol\;N\;g^{-1}\;FW\;h^{-1}$ and $12.7{\pm}10.1\;nmol\;P\;g^{-1}\;FW\;h^{-1}$. There were logarithmic relationships between thallus length and uptake rates of nutrients and $CO_2$, which suggested that younger specimens (<100-150 cm) were much more efficient at nutrients and $CO_2$ uptake than old specimens > 150 cm. There was a positive linear correlation ($r^2$=9.4) existed between the dissolved oxygen production rate and the $TCO_2$ uptake rate, suggesting that these two factors may serve as good indicators of S. japonica photosynthesis. There was also positive linear relationship between maximal quantum yield ($F_v/F_m$) and production/uptake rates of dissolved oxygen, $TCO_2$ and phosphate, suggested that $F_v/F_m$ could be used as a good indicator of photosynthetic ability and $TCO_2$ consumption of macroalgae. Maximum relative electron transport rate ($rETR_{max}$) of S. japonica increased as thallus grew and was high in distal part of thallus which may be resulted from the increase of photosynthetic cell density per area. The annual $TCO_2$ uptake by S. japonica in Gijang area was estimated about $1.0\sim1.7{\times}10^3C$ ton, which was about 0.02-0.03% of carbon dioxide emission in Busan City. Thus, more research should be focused on macroalgae-based biogeochemical cycles to evaluate the roles and contributions of macroalgae to the global carbon cycle.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.306-313
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• 2014

BACKGROUND: Forest soils contain microbes capable of consuming atmospheric methane ($CH_4$), an amount matching the annual increase in $CH_4$ concentration in the atmosphere. However, the effect of plant residue production by different forest structure on $CH_4$ oxidation is not studied in Korea. The objective of this study was to evaluate the effect of Korean alpine soils having different forestation structure on $CH_4$ uptake rates. METHODS AND RESULTS: the $CH_4$ flux was measured at three sites dominated with pine, chestnut and oak trees in southern Korea. The $CH_4$ uptake potentials were evaluated by a closed chamber method for a year. The $CH_4$ uptake rate was the highest in the pine tree soil ($1.05mg/m^2/day$) and then followed by oak ($0.930mg/m^2/day$) and chestnut trees ($0.497mg/m^2/day$). The $CH_4$ uptake rates were highly correlated to soil organic matter and moisture contents, and total microbial and methanotrophs activities. Different with the general concent, there was no any correlation between $CH_4$ oxidation rates, and soil temperature and labile carbon concentrations, irrespective with tree species. CONCLUSION: Conclusively, the methane oxidation rate was correlated in positive manner with organic matter, abundance of methanotrophs. Methane oxidation was different among tree species. This results could be used to estimate methane oxidation rate in forest of Korea after complementing information about statistical data and methane oxidation of other site.

• Ocean Science Journal
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• v.40 no.2
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• pp.79-89
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• 2005

Laboratory radiotracer experiments were conducted to determine assimilation efficiencies (AE) from ingested algal food and oxic sediment particles, uptake rates from the dissolved phase, and the efflux rates of Cd, Cr and Zn in the Asiatic clam Corbicula fluminea. Among three elements, AE from both algal and sediment food was greatest for Cd, followed by Zn and Cr. The AEs of tested elements from algal food (Phaeodactylum tricornutum) were consistently higher than those from sediments at a given salinity and temperature. The influence of salinity (0, 4 and 8 psu) and temperature (5, 13 and $21^{\circ}C$) on the metal AEs was not evident for most tested elements, except Cd AEs from sediment. The rate constant of metal uptake from the dissolved phase $(k_u)$ was greatest for Cd, followed by Zn and Cr in freshwater media. However, in saline water, the $(k_u)$ of Zn were greater than those of Cd. The influx rate of all tested metals increased with temperature. The efflux rate constant was greatest for Cr $(0.02\;d^{-1})$, followed by Zn $(0.010{\sim}0.017\;d^{-1})$ and $Cd\;(0.006\;d^{-1})$. The efflux rate constant for Zn in clam tissues depurated in 0 psu $(0.017\;d^{-1})$ was faster than that in 8 psu $(0.010\;d^{-1})$. Overall results showed that the variation of salinity and temperature in estuarine systems can considerably influence the metal bioaccumulation potential in the estuarine clam C. fluminea. The relatively high Cd accumulation capacity of C. fluminea characterized by the high AE, high dissolved influx rate and low efflux rate, suggested that this clam species can be used as an efficient biomonitor for the Cd contamination in freshwater and estuarine environments.

• Korean Journal of Environmental Biology
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• v.32 no.4
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• pp.297-305
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• 2014

In order to understand the contribution of seaweed aquaculture to nutrients and carbon cycles in coastal environments, we measured the nutrients & carbon uptake rates of Porphyra yezoensis Ueda sampled at Nakdong-River Estuary using a chamber incubation method from November 2011 to April 2012. It was observed that the production rate of dissolved oxygen by P. yezoensis (n=30~40) was about $68.8{\pm}46.0{\mu}mol\;{g_{FW}}^{-1}h^{-1}$ and uptake rate of nitrate, phosphate and dissolved inorganic carbon (DIC) was found to be $2.5{\pm}1.8{\mu}mol\;{g_{FW}}^{-1}h^{-1}$, $0.18{\pm}0.11{\mu}mol\;{g_{FW}}^{-1}h^{-1}$ and $87.1{\pm}57.3{\mu}mol\;{g_{FW}}^{-1}h^{-1}$, respectively. There was a positive linear correlation existed between the production rate of dissolved oxygen and the consumption rates of nitrate, phosphate and DIC, respectively, suggesting that these factors may serve as good indicators of P. yezoensis photosynthesis. Further, there was a negative logarithmic relationship between fresh weight of thallus and uptake rates of nutrients and $CO_2$, which suggested that younger specimens (0.1~0.3 g) were much more efficient at nutrients and $CO_2$ uptake than old specimens. It means that the early culturing stage than harvesting season might have more possibilities to be developed chlorosis by high rates of nitrogen uptake. However, N & C demanding rates of Busan and Jeollabuk-do, calculated by monthly mass production and culturing area, were much higher than those of Jeollanam-do, the highest harvesting area in Korea. Chlorosis events at Jeollabuk-do recently might have developed by the reason that heavily culture in narrow area and insufficient nutrients in maximum yield season (Dec.~Jan.) due mostly to shortage of land discharge and weak water circulation. The annual DIC uptake by P. yezoensis in Nakdong-River Estuary was estimated about $5.6{\times}10^3\;CO_2$ ton, which was about 0.03% of annual carbon dioxide emission of Busan City. Taken together, we suggest more research would be helpful to gain deep insight to evaluate the roles of seaweed aquaculture to the coastal nutrients cycles and global carbon cycle.

• Journal of the Korean Chemical Society
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• v.27 no.1
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• pp.31-37
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• 1983

The consumption of atmospheric carbon monoxide by soil was measured under laboratory conditions in different types of soils. Laboratory experiments were performed with humus containing high proportion of organic matter, roadside soils, and humus and roadside soils previously exposed to high concentration of CO by reusing in the experiment. CO concentrations in the 18.2 l-reaction vessel were varied from 2,000 ppm to 24,000ppm to estimate the effectiveness of CO consumption at high level of CO. The uptake of CO by soil was measured by gas chromatography using a TCD detector. The control experiments conducted along with the soil experiments evidently indicated that the potting soil is responsible for CO consumption. Humus showed much higher CO uptake rates compared with the soil taken from roadside. The humus reused in the experiment showed somewhat higher rates(15%) of uptake than the fresh one. The soil's ability to remove CO from the test atmosphere reached a maximum near the CO concentration of 13,000 ppm in the range of $9,000~24,000ppm$. The addition of streptomycin did not influence the removal capacity of soil significantly, whereas 10% saline solution remarkably prevented CO uptake of the humus sample.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.4
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• pp.266-271
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• 2001

Soybean can produce high-N residue due to N-fixation, so soybean rotation may increase yield of subsequent corn and reduce N fertilizer on the corn fairly. To find out the contribution of nitrogen to subsequent corn following soybean cultivation, soil nitrate, corn yield, and nitrogen uptake were measured for three continuous corn cropping years after soybean rotation. Three N rates of 0, 80, and 160 kg/ha were applied to three continuous corn following soybean cropping. At 6-leaf stage, soil nitrate amount at the soil depth of 0-30cm ranged from 60 to 80 kgN/ha higher in the first corn cropping year than that in the second and third corn cropping years. Judging from corn N status such as SPAD value, N concentration of ear-leaf and stover at silking stage, N contribution of previous soybean to corn in the first corn year was N fertilizer of approximately 80 kg N/ha. Stover N uptake at silking stage increased from 47 to 52 kg N/ha at the 0, and 80 kg N/ha of N rates in the first corn cropping year compared with those in the second and third corn cropping years. Corn grain yield at the 0 kg N/ha of N rate was 6-7 ton/ha higher in the first corn cropping year than that in the second and third corn cropping years, respectively. When compared the first corn year following soybean cropping with the second and third corn cropping years, N uptake of grain and stover at harvest with low N rates such as 0 and 80 kg N/ha increased from 45 to 67kg N/ha, from 35 to 60 kg N/ha, respectively. N uptake of whole plant by soybean rotation increased from 93 to 118 kg N/ha in the first year compared with that in the second and third corn cropping years. However, the N contribution by soybean cropping was small in the second and third continuous corn cropping years. Therefore, it was concluded that the nitrogen fertilizer of 80-100 kg N/ha in the first corn cropping year could be saved by soybean rotation and annual alternative corn-soybean rotation could be the best rotation system.

• Biomedical Science Letters
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• v.11 no.3
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• pp.327-332
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• 2005

Sf21 cells become popular as the host permissive cell line to support the baculovirus AcNPV replication and protein synthesis. The cells grow well on TC-100 medium that contains $0.1\%$ D-glucose as the major carbon source, strongly suggesting the presence of endogenous glucose transporters. However, unlike human glucose transporters, very little is known about the characteristics of the endogenoussugar transporter(s) in Sf21 cells. Thus, some kinetic properties of the sugar transport system were investigated, involving the uptake of 2-deoxy-D-glucose (2dG1c). In order to obtain a true measure of the initial rate of uptake, the uptake of $[^3H]2dGlc$ from both low $(100{\mu}M)$ and high (10 mM) extracellular concentrations was measured over periods ranging from 30 sec to30 min. The data obtained indicated that the uptake was linear for at least 2 min at both concentrations, suggesting that measurements made over a 1min time course would reflect initial rates of the jexpse uptake. To determine $K_m\;and\;V_{max}$ of the endogenous glucose transporter(s) in Sf21 cells, the uptake of 2dG1c was measured over a range of substrate concentrations $(50{\mu}M\~10mM)$ 2dG1c uptake by the Sf21 cells appeared to involve both saturable and non-saturable (or very low affinity) components. A saturable transport system for 2dG1c was relatively high, the $K_m$ value for uptake being < 0.45 mM. The $V_{max}$ value obtained for 2dG1c transport in the Sf21 cells was about 9.7-folds higher than that reported for Chinese hamster ovary cells, which contain a GLUT1 homologue. Thus, it appeared that the transport activity of the Sf21 cells was very high. In addition, the Sf21 glucose transporter was found to have very low affinity for cytochalasin B, a potent inhibitor of human erythrocyte glucose transporter