• Mycobiology
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• v.49 no.4
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• pp.346-354
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• 2021

The genus Aspergillus is commonly isolated from various marine and terrestrial environments; however, only a few species have been studied in rhizosphere soil. As part of the Korean indigenous fungal excavation project, we investigated fungal diversity from rhizosphere soil, focusing on Aspergillus species. A total of 13 strains were isolated from the rhizosphere soil of three different plants. Based on phylogenetic analysis of β-tubulin and calmodulin and morphological characteristics, we identified five Aspergillus species. A. calidoustus and A. pseudodeflectus were commonly isolated from the rhizosphere soil. Four species were confirmed as unrecorded species in Korea: A. calidoustus, A. dimorphicus, A. germanicus, and A. pseudodeflecuts. The detailed morphological descriptions of these unrecorded species are provided.

• The Korean Journal of Mycology
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• v.49 no.3
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• pp.393-397
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• 2021

The unrecorded fungus Galactomyces pseudocandidus de Hoog & M.T. Sm. was isolated and cultured from an introduced species, Procambarus clarkii, in Korea. Two related species, Galactomyces citri-aurantii and Geotrichum candidum, have been reported, but no other Galactomyces spp. have been found in Korea. In this study, we isolated G. pseudocandidus from the digestive tract of American crayfish using RGY (river water-glucose-yeast) medium and then compared the morphological and molecular characteristics of this species with those of related species. As a result, we confirmed that G. pseudocandidus is an unrecorded fungus in Korea, so we described and reported the cultural characteristics and microstructures, such as conidia.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.260-270
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• 2018

A study describing unrecorded and taxonomically undescribed indigenous species is in progress since 2006. Samples were collected from many sites in coastal waters and offshore of Korea as well as from Jeju Island. Since 2008, we have found ten unrecorded species of four genera belonging to family Dinophysaceae, Gonyaulacaceae, Gymnodiniaceae, and Heterocapsaceae. The species are as follows, Dinophysis elongata (2016 winter), D. nasuta (2016 winter), Gonyaulax alaskensis (2016 winter), G. diegensis (2017), G. monospina (2008), Amphidinium flagellans(2017), Heterocapsa circularisquama (2017), H. horiguchii (2017), H. lanceolata (2017), and H. pygmaea (2017) (note; The numbers in the parenthesis refer to the year in which the species was reported as unrecorded indigenous species by National Institute of Biological Resources, NIBR hereafter). Among them, seven species were described as newly recorded species in Korean waters, and three have been re-described in this study.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.299-307
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• 2018

Samples were collected from planktonic habitats of the fresh and brackish waters in Korea from August 2016 to May 2018. As a result, three genera and nine species were newly recorded in Korea. The unrecorded indigenous genera were Anathece, Chondrocystis and Geminocystis, and nine species were Anabaenopsis arnoldii, Anathece smithii, Chondrocystis dermochroa, Coelosphaerium aerugineum, Eucapsis microscopica, Geminocystis herdmanii, Microcystis panniformis, Synechococcus nidulans and Woronichinia karelica. Anathece smithii, Coelosphaerium aerugineum, Eucapsis microscopica, Microcystis panniformis and Synechococcus nidulans had been reported to inhabit freshwater, but these were found in brackish water in this study. Microcystis panniformis, which is a potential genus for causing green-tide, is taxonomically valuable in Korea.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.252-259
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• 2018

A research probing for the unrecorded and taxonomically undescribed indigenous species has been initiated since 2006. Samples were collected from an intertidal zone along the coasts of Korea as well as around the Jeju Island. We have found five unrecorded species of four genera belonging to the order Dinophysiales, Gymnodiniales, Peridiniales, and Prorocentrales. The species are as follows, Sinophysis canaliculata (2017) Bispidodinium angelaceum (2015), Cabra armorica (2016), Prorocentrum bimaculatum (2017), and P. tsawwassenense (2017) (note; The numbers in parenthesis refer to the year in which the species was reported as unrecorded indigenous species by National Institute of Biological Resources, NIBR hereafter).

• The Korean Journal of Mycology
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• v.49 no.1
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• pp.127-131
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• 2021

Lambertella corni-maris Höhn., an endophytic fungus that is an unrecorded species in Korea, was isolated and cultured from alpine coniferous Korean fir. The Lambertella sp. was found in Korean pine in Yeongwol-gun but no Lambertella spp. were recorded elsewhere in Korea. In this study, the morphological characteristics and molecular barcode sequence of L. cornimaris were compared to its related species. We tried to explain the cultural characteristics and microstructures, such as the conidia of L. corni-maris, compared to closely related taxa and present it.

• Journal of Species Research
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• v.10 no.4
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• pp.344-349
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• 2021

The purpose of this study was to isolate and identify wild yeasts from soil of Gyeongju city, and Haemadipsa rjukjuana of Gageodo Island, characterizing unrecorded yeast strains from Korea. The molecular analysis of the D1/D2 domain of 26S rDNA of yeast was performed using the Basic Local Alignment Search Tool (BLAST). No official report exists describing these three species: one species in the genus Candida, one species in the genus Debaryomyces, and one species in the genus Solicoccozyma. Candida saitoana YL9, Debaryomyces fabryi YL1, and Solicoccozyma terrea 20g9-1 are recorded for the first time from Korea. All three strains were oval shaped and polar binding, while positive for glucose, ᴅ-xylose, and ᴅ-cellobiose. Morphological, physiological, and biochemical properties are described in the species descriptions.

• Journal of Species Research
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• v.12 no.4
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• pp.355-361
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• 2023

In this paper, we present unrecorded protozoans of Korea that were discovered, through the 'Discovery of Korean Indigenous Species' project hosted by the 'National Institute of Biological Resources (NIBR)'. A taxonomic account is provided for each identified species, offering comprehensive information such as species name, Korean name, collection site, synonyms, specimen vouchers, diagnoses, and figures. This study introduces 13 previously unrecorded Korean protozoan species that are classified into three phyla: Amoebozoa, Cercozoa, and Ciliophora. Notably, the cercozoan family Chlamydophryidae was recorded in Korea for the first time, together with the discovery of three previously unreported genera: Diaphoropodon within Cercozoa, and Metauroleptus and Hemicycliostyla within Ciliophora.

• The Korean Journal of Mycology
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• v.51 no.4
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• pp.471-490
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• 2023

Soil is a rich source of saprobic and pathogenic fungi in crop cultivation areas. Compared with that of the fruit trees, scarce information is available regarding the fungi present in orchard soils in Korea. We sampled rhizosphere soils of apple and pear trees from several orchards in Cheongju, Anseong, and Cheonan, Korea. During the processing of soil fungi, seven unrecorded species of the phylum Ascomycota were isolated. These included Acrocalymma walkeri, Clonostachys krabiensis, Coniella vitis, Cosmospora diminuta, Lasiobolidium spirale, Penicillium vallebormidaense, and Pseudothielavia arxii. All the species were identified and described based on morphological characteristics and molecular analysis of the ITS1-5.8S-ITS2 region and the large subunit of nuclear ribosomal RNA gene and partial β-tubulin gene (BenA) sequences. Descriptions and illustrations of the morphological characteristics are provided.

• Journal of Species Research
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• v.13 no.2
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• pp.142-146
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• 2024

The purpose of this study was to isolate and identify wild yeasts from soil collected in Daegu City and Cheongyang County, Republic of Korea. Among 11 strains isolated in this study, nine strains were previously reported and two strains were unreported in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation test are done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. Of the two unrecorded yeast strains, CY-9-10C belongs to the genus Mrakia (family Mrakiaceae, order Cystofilobasidiales, class Tremellomycetes) and PG3-4-10C belongs to the genus Slooffia (family Chrysozymaceae, order Microbotryomycetes incertae sedis, class Microbotryomycetes). Both strains had oval-shaped and polar budding cells. This research described the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.