• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제36권6호
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• pp.453-459
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• 2010

Introduction: Ameloblastic carcinoma is a rare malignant lesion, and may arise from either carcinoma ex-ameloblastoma or de novo carcinoma. Aberrant promoter hypermethylation of the tumor-associated genes leading to their inactivation is a common event in many cancer types. The p16/CDKN2/INK4A gene and p16 5 protein are involved directly in regulating the cell cycles. Cadherins are cell adhesion molecules that modulate the epithelial phenotype and regulate tumor invasion. The aim of this study was to evaluate the roles of p16 and E-cadherin methylation and loss of p16 and E-cadherin expression in the malignant transformation of an ameloblastoma. Materials and Methods: Eight cases of ameloblastoma, including 4 benign ameloblastomas without recurrence, 2 benign ameloblastomas with recurrence and 2 carcinoma ex-ameloblastomas, were examined. The promoter hypermethylation profile of the p16 and E-cadherin genes was studied using methylation-specific polymerase chain reaction (MSP) and immunohistochemical staining for p16 and E-cadherin expression. Results: 1) Aberrant CpG island methylation of the p16 gene was detected in 3 of the 4 benign ameloblastomas without recurrence and 1 of the 2 benign ameloblastomas with recurrence. 2) Aberrant CpG island methylation of the E-cadherin gene was found in 1 of the 4 benign ameloblastomas without recurrence. 3) A loss of p16 expression was noted in 1 of 4 benign ameloblastomas without recurrence and 1 of 2 carcinoma ex-ameloblastomas. 4) A loss of E-cadherin expression was noted in 2 of the 4 benign ameloblastomas without recurrence, 1 of the 2 benign ameloblastomas with recurrence and 2 of the 2 carcinoma ex-ameloblastomas. 5) A loss of p16 expression was observed in 1 of the 4 cases showing aberrant methylation of the p16 gene. 6) A loss of E-cadherin expression was observed in 3 benign ameloblastoma case showing aberrant methylation of the E-cadherin gene. Conclusion: These results suggest that loss of E-cadherin expression related to the other genetic pathway (not methylation) might be an adjuvant indicator predicting the malignant transformation of an ameloblastoma. However, the number of samples in this study was too small and the relationship between the treatment methods and clinical course were not defined. Therefore, further study will be needed.

• Management Science and Financial Engineering
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• 제11권3호
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• pp.61-78
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• 2005

The aim of this paper is to identify the barriers to the B2B e-commerce development in China's pharmaceutical industry and to devise an effective strategy for its future development. Built on a detailed investigation of the market structure and recent development of electronic commerce in China's pharmaceutical industry, this paper proposes that the key issue in the development of effective B2B e-commerce business models is the successful transformation of the inter- organizational information systems into electronic marketplaces. In order to ensure the success of such electronic marketplaces, a government driven approach will be needed. In the process, designing an incentive compatible mechanism of coordinating the interest of all the market players will be the prerequisite.

• 산업공학
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• 제20권1호
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• pp.79-86
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• 2007

In B2B e-commerce environments, many initiatives for process modeling have made efforts to design business processes correctly. Especially, Petri nets have been widely used as a good theory to design and verify process models. Therefore, we propose the process modeling method for the B2B e-commerce based on Petri nets. First of all, a B2B process model based on BPSS is represented by the UML activity diagram. Second, the activity diagram is transformed to a Petri net model. For the transformation, well-behaved building blocks/control structures and the modeling rules for inter-organizational workflow processes are proposed. Third, the process is partitioned into sharable processes for individual business partners. Finally, according to needs of individual business partners, the sharable processes are modified by well-structured refinement rules. The whole procedure is explained with the purchase process of an e-bookstore.

• Structural Engineering and Mechanics
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• 제51권2호
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• pp.199-214
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• 2014

The present investigation is concerned with the effect of two temperatures on functionally graded (FG) nanobeams subjected to sinusoidal pulse heating sources. Material properties of the nanobeam are assumed to be graded in the thickness direction according to a novel exponential distribution law in terms of the volume fractions of the metal and ceramic constituents. The upper surface of the FG nanobeam is fully ceramic whereas the lower surface is fully metal. The generalized two-temperature nonlocal theory of thermoelasticity in the context of Lord and Shulman's (LS) model is used to solve this problem. The governing equations are solved in the Laplace transformation domain. The inversion of the Laplace transformation is computed numerically using a method based on Fourier series expansion technique. Some comparisons have been shown to estimate the effects of the nonlocal parameter, the temperature discrepancy and the pulse width of the sinusoidal pulse. Additional results across the thickness of the nanobeam are presented graphically.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.327-335
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• 2009

Creeping bentgrass (Agrostis stolonifera L.) is economically important as the principal turfgrass species for golf course greens and fairways in temperate climates around the world. As the utilization area of the turfgrass species increases recently, the demand for new and improved cultivars increases. Thus, substantial progress has been made in applying modern biotechnology to develop genetically engineered (i.e., biotech) creeping bentgrass with new traits that eluded the breeders. This review article addresses the advances made in developing biotech creeping bentgrass, which are categorized in the following topics: (i) genetic transformation of creeping bentgrass, (ii) development of various biotech creeping bentgrasses by genetic engineering, and (iii) progresses in the deregulation of herbicideresistant creeping bentgrass.

• 대한의생명과학회지
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• 제18권1호
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• pp.29-34
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• 2012

Streptomycetes are gram-positive filamentous bacteria that are well-known for producing a vast array of bioactive compounds, including more than 70 % of commercially important antibiotics. For the research about Streptomyces sp., the protoplast and electroporation transformation method have been the general techniques for the construction of transformants. However, these techniques have low efficiency and are time-consuming. Another option is intergenic conjugation, which is used for DNA transfer using methylation-deficient E. coli as a DNA donor to avoid the methylated-DNA-dependent restriction systems of actinomycetes. This conjugation method has been widely improved and applied to many other actinomycetes. In this research, an effective transformation procedure for the construction of expression vector by using gateway system was established to avoid limit of restriction enzyme site for cloning of target gene based on transconjugation by Escherichia coli ET12567/pUZ8002 with a pSET152 integration vector.

• Molecules and Cells
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• 제21권2호
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• pp.167-173
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• 2006

The literature provides strong precedent for both pro-tumorigenic and tumor suppressor roles for the c-Jun N-terminal kinases (JNKs) in the setting of oncogenesis. Clearly, JNKs are activated by numerous oncogenes and growth factors and the literature documents a role for these MAP kinases in cell proliferation and transformation. By contrast, JNKs mediate signals from diverse stimuli that result in cell death or differentiation and a role for JNKs as tumor suppressors has emerged. This enigmatic nature of the JNKs in the setting of oncogenesis is considered herein. Further illumination of the complex and context-dependent functions of the JNKs in cancer cells is of obvious importance for the rational use of small molecule JNK inhibitors as therapeutics.

• 대한수학회논문집
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• 제37권1호
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• pp.213-228
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• 2022

In the present paper, we aim to study Yamabe soliton and Riemann soliton on Lorentzian para-Sasakian manifold. First, we proved, if the scalar curvature of an 𝜂-Einstein Lorentzian para-Sasakian manifold M is constant, then either 𝜏 = n(n-1) or, 𝜏 = n-1. Also we constructed an example to justify this. Next, it is proved that, if a three dimensional Lorentzian para-Sasakian manifold admits a Yamabe soliton for V is an infinitesimal contact transformation and tr 𝜑 is constant, then the soliton is expanding. Also we proved that, suppose a 3-dimensional Lorentzian para-Sasakian manifold admits a Yamabe soliton, if tr 𝜑 is constant and scalar curvature 𝜏 is harmonic (i.e., ∆𝜏 = 0), then the soliton constant λ is always greater than zero with either 𝜏 = 2, or 𝜏 = 6, or λ = 6. Finally, we proved that, if an 𝜂-Einstein Lorentzian para-Sasakian manifold M represents a Riemann soliton for the potential vector field V has constant divergence then either, M is of constant curvature 1 or, V is a strict infinitesimal contact transformation.

• Molecules and Cells
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• 제21권1호
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• pp.21-28
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• 2006

The interferon-induced, double-stranded RNA (dsRNA)-dependent protein kinase PKR plays a key role in interferon-mediated host defense against viral infection, and is implicated in cellular transformation and apoptosis. We have isolated a cDNA of simian PKR encoding a product with 83% amino acid identity to the human homolog and showed that PKR expression is significantly attenuated in the Vero E6 African green monkey kidney cells devoid of type I interferon genes. A variant form of PKR lacking the exon 12 in the kinase domain is produced in these cells, presumably from an alternatively spliced transcript. Unlike wild type PKR, the variant protein named PKR-${\Delta}E12$ is incapable of auto-phosphorylation and phosphorylation of eIF2-${\alpha}$, indicating that the kinase sub-domains III and IV embedded in exon 12 are indispensable for catalytic function. PKR-${\Delta}E12$ had no dominant negative effect but was weakly phosphorylated in trans by wild type PKR.

• 열처리공학회지
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• 제11권2호
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• pp.111-120
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• 1998

The effects of chemical composition and thermal cycling on the martensitic transformation characteristics in Cu-rich, equiatomic and Zr-rich CuZr binary alloys have been studied by calorimetry. Only martensite could be indentified in equiatomic $Cu_{49.9}Zr_{50.1}$ alloy, while $Cu_{10}Zr_7$ and $CuZr_2$ intermetallic compounds as well as martensite were formed by rapid cooling from the melts in Cu-rich $Cu_{52.2}Zr_{47.5}$ alloy and Zr-rich $Cu_{48.4}Zr_{51.6}$ alloy, respectively. The $M_s$ temperature of $Cu_{49.9}Zr_{50.1}$ was $156^{\circ}C$ but those of $Cu_{52.5}Zr_{47.5}$ and $Cu_{48.4}Zr_{51.6}$ alloys, being $109^{\circ}C$ and $138^{\circ}C$, were lower than that of equiatomic $Cu_{49.9}Zr_{50.1}$ alloy. In all the alloys, the $M_s$ temperature has fallen but the $A_s$ temperature has risen, resulting in widening of the transformation hysteresis with thermal cycling. The anomalous characteristics in the transformation temperature are due to the presence of the intermetallic compounds i.e. $Cu_{10}Zr_7$ and $CuZr_2$ formed by an eutectoid reaction during thermal cycling in the temperature range between $-100^{\circ}C$ < $T_c$ < $400^{\circ}C$.