• Applied Biological Chemistry
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• v.41 no.5
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• pp.399-404
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• 1998

Genipin was obtained from hydrolysis of geniposide isolated from gardenia fruits with ${\beta}-glucosidase$. Reaction of genipin with glycine, alanine, histidine, lysine, phenylalanine and glutamate in aqueous buffer solution converted colorless starting materials to blue pigments. Effect of pH for the formation of blue pigments was tested using UV/Vis spectrophotometer. The optimum pH for the formation of blue pigments was 7.0. No pigment and trace amounts were formed at acidic (pH 3.0) and alkaline (pH 12.0) conditions, respectively. The amount and tincture of blue color were distinct with different amino acids. In contrast with lysine $({\lambda}_{max}=573\;nm)$, glycine $({\lambda}_{max}=595\;nm)$, phenylalanine $({\lambda}_{max}=602\;nm)$ and alanine $({\lambda}_{max}=595\;nm)$, the reaction of genipin with histidine $({\lambda}_{max}=601\;nm)$ and glutamate $({\lambda}_{max}=601\;nm)$ produced relatively small amounts of blue pigments. Rate constants for the formation of blue pigments from genipin with amino acids at various temperatures $(60,\;70,\;80,\;90^{\circ}C,\;pH\;7.0\;phosphate\;buffer)$ were obtained. Rate constants of genipin with basic amino acids were larger than neutral or acidic amino acids. Arrhenius activation energies of the formation of blue pigments indicated that activation energy of glycine $(E_A=9.8\;kcal/mol)$ was especially lower than those of other amino acids $(E_A=13.3{\sim}15.4\;kcal/mol)$.

• Sim-seong Yeon-gu
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• v.25 no.1
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• pp.27-64
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• 2010

The main focus of this article investigates Jung's analytic implications of the Spiritual Exercises by St. Ignatius of Loyola. The Exercises is referred to not only as the tool for transformation that transformed Ignatius from a soldier of the world into a soldier of God and led him to a completely changed life but also as a tool which galvanizes self-realization, i.e., individuation process, in which a faithful experiences the presence of God in his life and is in search for himself in a new way. The interest in the Exercises regarded as a Western version of Yoga of the East which is a tool for transformation led Jung to give a series of 20 lectures on the Exercises in a seminar held in Zurich from 1939 to 1940. Curiosity about Jung's understanding on the Exercises provokes my desire to step into this research. The Exercises is a book for spiritual exercises that prepare and dispose one's soul to rid itself of all disordered attachments and to order one's life. The Exercises is made up of four Weeks. The First begins with 'Principle and Foundation' which illustrates what human beings are created for. It leads retreatants to rid themselves of disordered attachments and to have a new perspective on life by the consideration and contemplation of sins as the subversion of the Principle and Foundation. The Second is the period in which retreatants accept Christ as the Master of their lives through the meditation and contemplation of the life of Christ. In the Third, retreatants take part in the salvation history of Christ not only by actively participating in the Passion of Christ but also by incorporating the Passion into their lives. The Fourth aids retreatants to undergo their transformation and experience it deeply in order to participate in the new life of Christ who by His resurrection overcame death. In conclusion, Jung viewed the Exercises as a Western tool which plays the similar role of Yoga of the East which engenders inner transformation. The four-week-long retreat helps retreatants to meditate on God who unifies everything and is Himself/Herself the perfect union or the unity so that imperfect retreatants are given opportunities to undergo complete metamorphosis into the immortal, indivisible, and impeccable God. Jung understood that this metamorphosis leads human beings to the totality, that is, the genuine self as the image of God. The author interprets that it is the transformation that the Exercises tries to attain, which resonates with individuation, the key element of analytic psychology.

• Journal of Korean Society of Environmental Engineers
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• v.34 no.7
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• pp.445-453
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• 2012

An investigation for removal of 1-indanone (1-ID), which were commonly produced from the biological and/or chemical treatment and natural weathering of the PAHs-contaminated soils, via oxidative transformation mediated by birnessite in the presence of various phenolic mediators is described. This study also examines the potential effect of the natural occurring substance humic acid (HA) on the oxidative transformation. The experiment was carried out in aqueous phase as a batch test (10 mg/L 1-ID, 0.3 mM phenolic mediators, $1.0g/L\;{\delta}-MnO_2$, at pH 5). All of the 11 tested phenoilic mediators belong to the group of natural occurring phenols and are widely used as model constituents of humic substances. From the results of HPLC analysis, it is demonstrated that 1-ID was not reactive to birnessite itself, but it can be effectively removed in birnessite-mediated cross coupling reactions in the presence of the phenolic mediators. The percent removals of 1-ID after 2 day incubation were ranged from 9.2 to 71.2% depending on the phenolic mediators applied. The initial rate constant ($K_{int}$, $hr^{-1}$) values for the 1-ID removals obtained from the pseudo-first-order kinetic plots also widely ranged from 0.18 to 15.0. Results of the correlative analysis between the removal efficiencies and structural characteristics of phenolic mediators indicate that the transformation of the 1-ID was considerably enhanced by the addition of electron-donating substituents (e.g., -OH, $-OCH_3$) at the benzne ring, and much less enhanced by the addition of electron-withdrawing substituents (e.g., -COOH, -CHO). The presence of HA showed that removal efficiencies of 1-ID in the birnessite-phenolic mediator systems decreased with increasing HA concentrations. However at low concentration of HA (< 2 mg/L), it caused some enhancement in the removals of 1-ID as compared to the control.

• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.174-185
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• 2010

Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. To elucidate the functions of a large population of Chinese cabbage (Brassica rapa) genes and to search efficiently for agriculturally useful genes, we have been taking advantage of the full-length cDNA Over-eXpresser (FOX) gene hunting system. With oligo dT column it purify the each mRNA from the flower organs, leaf and stem tissue. And about 120,000 cDNAs from the library were transformed into $\lambda$-pFLCIII-F vector. Of which 115,000 cDNAs from the library were transformed into T-DNA binary vector, pBigs for transformation study. We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. Full-length Chinese cabbage cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Selfed seeds were harvested from transgenic Arabidopsis. We had selected 2,500 transgenic plants by hygromycin antibiotic tolerant test, and obtained a number of transgenic mutants. Each transgenic Arabidopsis was investigated in morphological changes, fertility and leaf colour. As a result, 285 possible morphological mutants were identified. Introduced cDNA was isolated by PCR amplification of the genomic DNA from the transgenic mutants. Sequencing result and BLAST analysis showed that most of the introduced cDNA were complete cDNAs and functional genes. Also, we examined the effect of Bromelain on enhancing resistance to soft rot in transgenic Chinese cabbage 'Osome'. The bromelain gene identified from FOX hunting system was transformed into Chinese cabbage using Agrobacterium methods. Transformants were screened by PCR, then RT-PCR and real time PCR were performed to analyze gene expression of cysteine protease in the T1 and T2 generations. The anti-bacterial activity of bromelain was tested in Chinese cabbages infected with soft rot bacteria. The results showed that the over-expressed bromelain gene from pineapple conferred enhanced resistance to soft rot in Chinese cabbage.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.1042-1047
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• 2004

Voltage control is an essential part of the electric energy transmission and distribution system to maintain proper voltage limit at the consumer's terminal. Besides the generating units that provide the basic voltage control, there are many additional voltage-controlling agents e.g., shunt capacitors, shunt reactors, static VAr compensators, regulating transformers mentioned in [1], [2]. The most popular one, among all those agents for controlling voltage levels at the distribution and transmission system, is the on-load tap changer transformer. It serves two functions-energy transformation in different voltage levels and the voltage control. Artificial Neural Network (ANN) has been realized as a convenient tool that can be used in controlling the on load tap changer in the distribution transformers. Usage of the ANN in this area needs suitable training and testing data for performance analysis before the practical application. This paper briefly describes a procedure of processing the data to train an Artificial Neural Network (ANN) to control the tap changer operating decision of parallel transformers for a closed primary bus. The data set are used to train a two layer ANN using three different neural net learning algorithms, namely, Standard Backpropagation [3], Bayesian Regularization [4] and Scaled Conjugate Gradient [5]. The experimental results are presented including performance analysis.

• BMB Reports
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• v.33 no.2
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• pp.162-165
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• 2000

Polymerase chain reaction (PCR) is well established as an indispensable tool of molecular biology; and yet a limitation for cloning applications continues to be that products often require subsequent restriction to be that products often require subsequent restriction digests, blunt-end ligation, or the use of special linear vectors. Here a rapid, PCR-based system is described for the simple, restriction enzyme-free generation of synthetic, 'restriction-like' DNA fragments with staggered ends. Any 3'- or 5'-protruding terminus, but also non-palindromic overhangs with an unrestricted single strand length are specifically created. With longer overhangs, "Restriction-PCR" does not even require a ligation step prior to transformation. Thereby the technique presents a powerful tool e.g. for a successive, authentic reconstitution of sub-fragments of long genes with no need to manipulate the sequence or to introduce restriction sites. Since restriction enzyme-free and thereby devoid the limitations of partial DNA digests, "Restriction-PCR" allows a straight one-step generation and cloning of difficult DNA fragments that internally carry additional sites for specific sequence insertions or deletions can be precisely engineered into genes of interest. With these properties "Restriction-PCR" has the potential to add significant speed and versatility to a wide variety of DNA cloning applications.

• 한국해양학회지
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• v.8 no.2
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• pp.68-74
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• 1973

The present paper concerns the age determination and growth of butter fish, Pampus argenteus from the southwestern waters of Korea by otolith reading. 743 specimens taken by stow-net in the southern part of the Yellow Sea and northeastern part of the East China Sea during the period from October 1972 to September 1973 were examined. Results of the study are summarized as follows: 1. Sex ratio of females to males was found to be 2:1. 2. Ring marks on the otolith were found to be formed twice a year, once during the period of January-May and the other time in September. 3. The Lee's phenomenon was observed on the otolith sample. 4. The relationship between the radius of otolith(R) and fork length(L) was found to be as follows: R=0.3069+0.0133L 5. Calculated fork length at the time of otolith ring formation are found to be as follows:I-ring,71.67mm; II-ring, 125.05mm; III-ring, 168.65mm; IV-ring, 201.74mm; V-ring, 225.80mm; VI-ring, 240.84mm. 6. Maximum fork length calculated according to the diagram of Walford's growth transformation was found to be 281.5mm. 7. Growth curve, when related to the von Bertalanffy's equation, was laid out as $L_{t}=281.5[1-e$^{-0.674(t-0.128)}]$

• The Journal of Internal Korean Medicine
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• v.12 no.2
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• pp.138-147
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• 1991

Review of literature on stomachache. According to investigated the pathology of a stomachache about all sorts of falling ill, I have been obtained following conclusions. 1. The cause of Ui Wan Tong are clod-wet-ui wan, sik juk, dam eum, a huy1 be made of gi bul soon (氣不順). 2. Pain of Je sang bu are called jung wan tong, dae bok tong, jung cho tong, cause are sik juk, han sa, chung juk. pain of je ha bu are called so bok tong, hache tong, soh bok tong, so bok are devided two part, one is so eum sin the other is gul eum gan, cause are jo gyul dae jang, yul gyu1 bang kwang, hyul gyul bang kwang, porak hu ect. 3. Pain of je bu are called je bok tong, hwan je e tong, so bok je ju tong, and cause are juk yu1, jo si, dam hwa. Pain of yang hyup bu are called yang hyup ha so bok tong, yang bang ge hyup tong, yang hup jisang tong, cause are ban gi,yang myung jo geom. According to above results, the patholosy of a stomach ache about the part of falling ill in a course of transformation on the epidemic fever comes into the viscera.

• Proceedings of the CALSEC Conference
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• 2005.03a
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• pp.130-134
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• 2005

This research describes the comparison broker's role and its effectiveness measurement using a developed logical framework of comparison shopping service. And verifies that seller-led comparison challenge method provide comparison information of products to buyers more efficiently. In electronic commerce, buyer's satisfaction of purchase (S) can be defined as an interactive function between seller's competitiveness vector (P) of products that supplied to the market, and buyer's informed level vector (B) of products that is known from a lot of sources. Then the buyer's informed level can be changed through the information analysis among products by transformation process using comparison matrix (C). So the role of comparison shopping is to construct a comparison matrix and to serve it to the buyers, and to change the buyer's informed level. The changed informed level influences a buyer's satisfaction, that improved satisfaction of purchase is defined as the effectiveness of comparison shopping. As a perfect provision and usage of comparison matrix is impossible cause of cognitive limit, the most efficient method for improving the comparison effectiveness is the comparison challenge that detects the comparison elements of the largest buyer's information efficiency, and then to be compared between elementary products selectively. This research verifies the substantial superiority of comparison challenge through television market data experiments.

• Journal of Institute of Control, Robotics and Systems
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• v.8 no.1
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• pp.37-45
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• 2002

A method of measuring the length of defects on the wall and restructuring the defect image is proposed based on the estimation algorithm of a camera orientation, which uses the declination angle of a laser slit beam. The estimation algorithm of the horizontally inclined angle of CCD camera adopts a 3-dimensional coordinate transformation of the image plane where both the laser beam and the original image of the defects exist. The estimation equation is obtained by using the information of the beam projected on the wall and the parameters of this equation are experimentally obtained. With this algorithm, the original image of the defect can be reconstructed as an image normal to the wall. From the result of a series of experiments, the measuring accuracy of the defect is measured within 0.5% error bound of real defect size under 30 degree of the horizontally inclined angle. The proposed algorithm provides the method of reconstructing the image taken at any arbitrary horizontally inclined angle as the image normal as the wall and thus, it enables the accurate measurement of the defect lengths by using a single camera and a laser slit beam.