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Draft Genome Sequence of the White-Rot Fungus Schizophyllum Commune IUM1114-SS01

  • Kim, Da-Woon;Nam, Junhyeok;Nguyen, Ha Thi Kim;Lee, Jiwon;Choi, Yongjun;Choi, Jaehyuk
    • Mycobiology
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    • v.49 no.1
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    • pp.86-88
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    • 2021

  • The monokaryotic strain, Schizophyllum commune strain IUM1114-SS01, was generated from a basidiospore of dikaryotic parental strain IUM1114. It even showed the decolorizing activities for several textile dyes much better than its parental strain. Based on the results of a single-molecule real-time sequencing technology, we present the draft genome of S. commune IUM1114-SS01, comprising 41.1 Mb with GC contents of the genome were 57.44%. Among 13,380 protein-coding genes, 534 genes are carbon hydrate-active enzyme coding genes.

  • https://doi.org/10.1080/12298093.2020.1843222 인용 PDF KSCI

Expression profiles of microRNAs in skeletal muscle of sheep by deep sequencing

  • Liu, Zhijin;Li, Cunyuan;Li, Xiaoyue;Yao, Yang;Ni, Wei;Zhang, Xiangyu;Cao, Yang;Hazi, Wureli;Wang, Dawei;Quan, Renzhe;Yu, Shuting;Wu, Yuyu;Niu, Songmin;Cui, Yulong;Khan, Yaseen;Hu, Shengwei
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.6
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    • pp.757-766
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    • 2019

  • Objective: MicroRNAs are a class of endogenous small regulatory RNAs that regulate cell proliferation, differentiation and apoptosis. Recent studies on miRNAs are mainly focused on mice, human and pig. However, the studies on miRNAs in skeletal muscle of sheep are not comprehensive. Methods: RNA-seq technology was used to perform genomic analysis of miRNAs in prenatal and postnatal skeletal muscle of sheep. Targeted genes were predicted using miRanda software and miRNA-mRNA interactions were verified by quantitative real-time polymerase chain reaction. To further investigate the function of miRNAs, candidate targeted genes were enriched for analysis using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment. Results: The results showed total of 1,086 known miRNAs and 40 new candidate miRNAs were detected in prenatal and postnatal skeletal muscle of sheep. In addition, 345 miRNAs (151 up-regulated, 94 down-regulated) were differentially expressed. Moreover, miRanda software was performed to predict targeted genes of miRNAs, resulting in a total of 2,833 predicted targets, especially miR-381 which targeted multiple muscle-related mRNAs. Furthermore, GO and KEGG pathway analysis confirmed that targeted genes of miRNAs were involved in development of skeletal muscles. Conclusion: This study supplements the miRNA database of sheep, which provides valuable information for further study of the biological function of miRNAs in sheep skeletal muscle.

  • https://doi.org/10.5713/ajas.18.0473 인용 PDF KSCI

Actinidia arguta Sprout as a Natural Antioxidant: Ameliorating Effect on Lipopolysaccharide-Induced Cognitive Impairment

  • Kang, Jeong Eun;Park, Seon Kyeong;Kang, Jin Yong;Kim, Jong Min;Kwon, Bong Seok;Park, Sang Hyun;Lee, Chang Jun;Yoo, Seul Ki;Heo, Ho Jin
    • Journal of Microbiology and Biotechnology
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    • v.31 no.1
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    • pp.51-62
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    • 2021

  • Here, we investigated the prebiotic and antioxidant effects of Actinidia arguta sprout water extract (AASWE) on lipopolysaccharide (LPS)-induced cognitive deficit mice. AASWE increased viable cell count, titratable acidity, and acetic acid production in Lactobacillus reuteri strain and showed a cytoprotective effect on LPS-induced inflammation in HT-29 cells. We assessed the behavior of LPS-induced cognitive deficit mice using Y-maze, passive avoidance and Morris water maze tests and found that administration of AASWE significantly improved learning and memory function. The AASWE group showed antioxidant activity through downregulation of malondialdehyde levels and upregulation of superoxide dismutase levels in brain tissue. In addition, the AASWE group exhibited activation of the cholinergic system with decreased acetylcholinesterase activity in brain tissue. Furthermore, AASWE effectively downregulated inflammatory mediators such as phosphorylated-JNK, phosphorylated-NF-κB, TNF-α and interleukin-6. The major bioactive compounds of AASWE were identified as quercetin-3-O-arabinopyranosyl(1→2)-rhamnopyranosyl(1→6)-glucopyranose, quercetin-3-O-apiosyl(1 → 2)-galactoside, rutin, and 3-caffeoylquinic acid. Based on these results, we suggest that AASWE not only increases the growth of beneficial bacteria in the intestines, but also shows an ameliorating effect on LPS-induced cognitive impairment.

  • https://doi.org/10.4014/jmb.2009.09012 인용 PDF KSCI