• Transactions of the Korean Society of Mechanical Engineers B
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• v.35 no.6
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• pp.593-601
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• 2011

An analysis program to simulate the operation of a polymer electrolyte membrane fuel cell (PEMFC) system was set up, and system operation with variations in the working conditions of various components (especially the thermal management system) was simulated. The entire system included a PEMFC stack and balance-of-plant components such as an air-supply unit, a fuel-supply unit, and a heat-management unit (cooling system). Thermodynamic models of all components were made to evaluate the design performance of the entire system, and then off-design models were set up to simulate the operation of the entire system under arbitrary working conditions. A parametric study was carried out to examine the effects of varying the operating conditions (especially the ambient conditions and the operating conditions of the cooling system) on the operation and performance of the entire system.

• Journal of the Korean Association of Geographic Information Studies
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• v.6 no.3
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• pp.1-10
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• 2003

RUSLE(revised universal soil loss equation) has been widely used for estimating soil loss. It is very difficult to validate the model estimation since the calculated soil loss should be compared with the survey data for quantification. The input variables for RUSLE model were estimated to grid cell for raster analysis in Bosung basin. Both reconnaissance(1:250,000) and detailed(1:25,000) soil maps were used to derive the input variables for soil erodibility factor. Soil loss calculated using RUSLE were compared to the unit sediment deposit surveyed by KICT(Korea Institute of Construction Technology, 1992) in Bosung basin for assessment. Unit sediment deposit from the cell size of 120m and 150m were the closest to the survey data in 1:250,000 and 1:25,000 map scale, respectively.

• KSBB Journal
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• v.26 no.5
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• pp.427-432
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• 2011

In this study, a EXGA gene code for exo-β-1,3-glucanase from Aspergillus oryzae was overexpressed and secretory produced in Saccharomyces cerevisiae. To overexpress the β-1,3-glucanase, pGInu-exgA and pAInu-exgA plasmids having GAL10 and ADH1 promoter, respectively, and exoinulinase signal sequence (Inu s.s) were constructed and introduced in S. cerevisiae SEY2102 and 2805. The recombinant β-1,3-glucanase was successfully expressed and secreted into the medium and the β--1,3-glucanase activity in 2102/pGInu-exgA and 2102/pAInu-exgA strain were 5.01 unit/mL and 4.09 unit/mL, respectively. In the 2805/pGInu-exgA and 2805/pAInu-exgA strain, the β-1,3-glucanase activity showed 3.23 unit/mL and 3.22 unit/mL, respectively. Secretory efficiency in each strain reached 95% to 98%. Subsequently, the recombinant β1,3-glucanase was used for ethanol production. Ethanol productivity in 2102/pAInu-exgA strain was 0.83 g/L when pre-treated Laminaria japonica which has initial reducing sugar of 1.4 g/L was used as substrate. It is assumed that the polysaccharides of Laminaria japonica was effectively saccharified by recombinant β-1,3-glucanase, resulting in increase of ethanol productivity. These results suggested that recombinant β-1,3-glucanase was efficiently overexpressed and secreted in S. cerevisiae SEY2102 as host strain by using ADH1 promoter-Inu s.s system.

• Journal of the Korea Convergence Society
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• v.10 no.4
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• pp.139-145
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• 2019

This paper investigated the strength and stiffness of composite truss unit structures. The model used is a core-filled model combining the Kagome model and the cube truss model. The material properties used for the analysis are 304 stainless steel with elastic modulus of 193 GPa and yield stress of 215 MPa. The theoretical equation is derived from the relative elasticity relation of Gibson - Ashby ratio, the analysis was performed using Deform 3D, a commercial tool. In conclusion, the relative elasticity for this unit model correlates with 1.25 times the relative density and constant coefficient, elasticity is inversely proportional to pore size. The relative compressive strength has a correlation with relative density of 1.25 times. Proof of this is a real experiment, the derived theoretical relationship should further consider mechanical behavior such as bending and buckling. In the future, it is hoped that the research on the elasticity and the stress according to the structure of the three-dimensional space will be continued.

• The Journal of Advanced Prosthodontics
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• v.13 no.2
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• pp.71-78
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• 2021

Purpose. This study aims to compare the volumetric change, degree of conversion (DOC), and cytotoxicity of 3D-printed restorations post-cured under three different conditions. Materials and Methods. 3D-printed interim restorations were post-cured under three different conditions and systems: 5 min, 30 min, and 24 h. Three-unit and six-unit fixed dental prostheses (n = 30 for each case) were printed; ten specimens from each group were post-cured and then scanned to compare their volumetric changes. Root-mean-squared (RMS) values of the data were acquired by superimposing the scanned files with original files. Thirty disk-shaped specimens were printed to evaluate the DOC ratio. Fourier transform infrared spectroscopy was used to compare the DOCs of 10 specimens from each group. Human gingival fibroblasts were used to measure the cell viability of every specimen (n = 7). The data from this experiment were employed for one-way analysis of variance and Tukey's post-hoc comparisons. Results. Differences between the three-unit restorations were statistically insignificant, regardless of the post-curing conditions. However, for the six-unit restorations, a high RMS value was acquired when the post-curing duration was 30 min. The average DOC was approximately 56 - 62%; the difference between each group was statistically insignificant. All the groups exhibited cell viability greater than 70%, rendering them clinically acceptable. Conclusion. The post-curing conditions influenced the volume when the length of the restoration was increased. However, this deviation was found to be clinically acceptable. Additionally, post-curing did not significantly influence the DOC and cytotoxicity of the restorations.

• Proceedings of the KIPE Conference
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• 2001.10a
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• pp.639-643
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• 2001

Recently, photovoltaic system has been studied widely as a renewable energy system, because it does not produce environmental pollution and it has infinity energy source from the sun. A study on photovoltaic system has a lot of problems like as reappearance and repetition of some situation in the laboratory experiment for development of MPPT algorithm and islanding detection algorithm, because output characteristics of solar cell are varied by irradiation and surface temperature of solar cell. And this system is consisted a lot of solar cell unit. Therefore, the assistant equipment which emulates the solar cell characteristics which can be controlled arbitrarily by researcher is require to the researchers for reliable experimental data. In this paper, the virtual implement of solar cell (VISC) system is proposed to solve these problems and to achieve reliable experimental result on photovoltaic system. VISC system emulates the solar cell output characteristics, and this system can substitute solar cell in laboratory experiment system. To realize the VISC, mathematical model of solar cell is studied for driving converter and the DC/DC converters are compared in viewpoint of tracking error using computer simulation. And then analysis of parallel and series characteristics was done for combination of VISC model.

• Journal of Microbiology and Biotechnology
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• v.8 no.1
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• pp.42-48
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• 1998

In order to maximize the secretory expression of human serum albumin (HSA) in the yeast Saccharomyces cerevisiae, a series of HSA expression vectors were constructed with a combination of different promoters, 5' untranslated regions (5'UTR), and secretion signal sequences. The expression vector composed of the galactose-inducible promoter GALl0, the natural 5'UTR, and the natural signal sequence of HSA directed the most efficient expression and secretion of HSA among the constructed vectors when introduced into several S. cerevisiae strains. Although the major form of HSA expressed and secreted in the yeast transformants was the mature form of 66 kDa, the truncated form of 45 kDa was also detected both in the cell extract and in the culture supernatant. The level of the intact HSA protein in the culture supernatant reached up to 30 mg/l at 24 h of cultivation in a shake-flask culture but began to decrease afterwards, indicating that the secreted HSA protein was unstable in a prolonged culture of yeast.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.8 no.4
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• pp.97-103
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• 2009

Since a demand for an extensive range of earthmoving equipment like a hydraulic excavator with a capacity of 85tons has been asked in construction fields, a performance of its track drive unit has to be verified experimentally. Among the verification of the performances, a torque measurement is at issue, in which a torque meter is utilized widely. However, the very large scaled torque meter is necessary when a discharged torque from the track drive unit is increased significantly. In addition, the price for experimental set-up of a torque meter is too high due to its limitation of the geometry such as long length, and a break down in operation occurs frequently. In this study, to measure a high torque up to 200,000Nm, a new technique was proposed as an alternative of conventional measurement by using a torque meter. The new technique enables to measure the high torque stably in a compact space via a torque arm and two load cells. The experimental results showed a propriety of the proposed torque measuring technique for a track drive unit with very large capacity.

• Korean Journal of Pharmacognosy
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• v.29 no.2
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• pp.79-85
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• 1998

Six antioxidative compounds in the aerial parts of Potentilla fragarioides were isolated by a bioassay guided purification using a DPPH free radical. They were identified as (+)-catechin, isoquercitrin, quercitrin, $quercetin-3-O-{\beta}-D-glucopyranosyl-{\beta}-D-xylopyranoside$, caffeic acid, and 4-O-caffeoyl-L-threonic acid on the basis of $^{1}H$ and $^{13}C-NMR$ and MS data. The DPPH radical scavenging activity of five compounds $(RC_{50}:\;7.5{\sim}10.5\;{\mu}g)$ except for quercitrin $(16\;{\mu}g)$ was more effective than those of ${\alpha}-tocopherol$ $(12\;{\mu}g)$ and BHA $(14\;{\mu}g)$.

• Proceedings of the Korea Society for Energy Engineering kosee Conference
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• 1998.05a
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• pp.259-263
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• 1998

SOFC가 실제 발전 목적으로 사용되려면 SOFC의 성능 및 수명의 향상과 아울러 발전 목적에 따른 대용량화가 요구된다. 분산형 전원으로 SOFC를 사용하게 될 경우 수백 kW 이상의 발전 용량이 요구되며, 이러한 대형 SOFC 발전 시스템은 수십 ㎾급 SOFC를 기본 단위로 하여 이들을 서로 연결함으로써 구성 가능하다. 평판형 SOFC의 대형화는 기본적으로 개별 전지의 전극 면적을 확대하여 출력 전류를 증가시키고, 단위 전지를 여러 단 적층하여 출력 전압을 증가시킴으로써 가능해 진다. (중략)