• Biomolecules & Therapeutics
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• v.17 no.2
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• pp.138-143
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• 2009

We have reported previously on a replication incompetent recombinant adenoviral vector, AdLPCD, in which the expression of cytosine deaminase gene (CD) is driven by the tumor-specific L-plastin promoter. AdLPCD vector had been evaluated for its efficacy of chemosensitization of ovarian cancer cells to 5-FC. In spite of the fact that ovarian cancer cells, i.e., OVCAR-3 and SK-OV-3, are capable for adenoviral transduction judged by LacZ reporter gene analysis, two cell lines demonstrated quite different sensitivities toward AdLPCD/5-FC system. In OVCAR-3 cells, infection of AdLPCD followed by exposure to 5-FC resulted in the suppression of cell growth with statistical significance. On the other hand, SK-OV-3 cells were more resistant to the CD/5-FC strategy compared with OVCAR-3 cells under the same condition. The object of study was to investigate factors that would determine the sensitivity to AdLPCD/5-FC. We evaluated conversion rate of 5-FC to 5-FU after infection of AdLPCD by HPLC analysis, $IC_{50}$ of 5-FU, the expression level of integrin receptors i.e., ${\alpha}v{\beta}3$ and ${\alpha}v{\beta}5$, and status of p53 in OVCAR-3 and SK-OV-3 cells. The results indicated that OVCAR-3 cells have few favorable features compared with SK-OV-3 cells to be more effective to the AdLPCD/5-FC strategy; higher level of ${\alpha}v{\beta}5$ integrin, higher rate of conversion of 5-FC into 5-FC, and lower $IC_{50}$ of 5-FU. The results suggest that the replacement of 5-FU with CD/5-FC in combination chemotherapy would be less toxic and much greater cytotoxicity than the conventional combination chemotherapy in some patients.

• Journal of KIISE:Computing Practices and Letters
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• v.10 no.1
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• pp.57-65
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• 2004

In the block matching algorithm, search patterns of different shapes or sizes and the distribution of motion vectors have a large impact on both the searching speed and the image quality. In this paper, we propose a new fast block matching algorithm using the unit-diamond search pattern and the flat-hexagon search pattern. Our algorithm first finds the motion vectors that are close to the center of search window using the unit-diamond search pattern, and then fastly finds the other motion vectors that are not close to the center of search window using the flat-hexagon search pattern. Through experiments, compared with the hexagon-based search algorithm(HEXBS), the proposed unit-diamond and flat-hexagonal pattern search algorithm(UDFHS) improves as high as 11∼51% in terms of average number of search point per motion vector estimation and improves about 0.05∼0.74㏈ in terms of PSNR(Peak Signal to Noise Ratio).

• Journal of IKEEE
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• v.18 no.2
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• pp.282-290
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• 2014

This paper presents the design of embedded electrical power control unit for Personal Electrical Vehicle(PEV). The embedded unit is designed using PIC18F8720 processor, 16Mb flash ROM, 32Mb SDRAM and signal condition circuits. The proposed PEV consists of 4KW in-wheel Brushless DC Motor(BLDCM), 3 phase voltage source inverter with the $180^{\circ}$ conduction space vector PWM method, PID speed controller and the embedded control unit. The PEV has mechanical manufacture of inverse 3 wheel system, which is applied by the in-wheel BLDCM and steering mechanism with tilting function. Also, the performances of the proposed embedded electrical power control unit are verified through the lab experiment and road driving test of PEV.

• Microbiology and Biotechnology Letters
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• v.33 no.4
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• pp.281-287
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• 2005

The endoinulinase gene (inu, 2.733 kb, EC 3.2.1.7) from Paenibacillus polymyxa was subcloned into an Escherichia coli-yeast shuttle vector with GALl promoter for the expression in Saccharomyces cerevisiae. The constructed plasmid, pYGENIU27 (8.6 kb) was introduced into S. cerevisiae SEY2102 cell and then the yeast transformant was selected on the synthetic defined media lacking uracil and on the inulin-containing media. The recombinant endoinulinase was predominantly localized in the periplasmic space of the yeast cell. The total activity of the endoinulinase reached 1.81 unit/ml by cultivation of yeast transformant on YPDG medium. The optimized conditions determined for the inulooligosaccharides (IOSs) production from inulin were as follows; pH, 8.0; reaction temperature, $45^{\circ}C$; inulin source, Jerusalem artichoke. Enzyme activity was stably maintained up to the pH of 10.0. Under the optimized condition and with endoinulinase of 36 unit/g-inulin, IOSs started to be produced after 10 min of enzymatic reaction. By the reaction with inulin, IOSs consisting of inulobiose (F2), inulotriose (F3), and inulotetraose (F4) were produced and F3 was the major product. Consequently, these data would be used as a fundamental parameters for the production of functional sweetener IOSs from inulin by recombinant yeast endoinulinase.

• Journal of Life Science
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• v.15 no.1 s.68
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• pp.118-123
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• 2005

For the expression in Saccharomyces cerevisiae, Bacillus stearothermophilus cyclodextrin glucano­transferase gene (cgtS) in pCGTS (4.8 kb) was subcloned into the surface expression vector, pYD1 (GALl promoter). The constructed plasmid, pYDCGT (7.2 kb) was introduced into S. cerevisiae EBY100 cells, and then yeast transformants were selected on the synthetic defined media lacking tryptophan. The formation of cyclodextrin (CD) was confirmed with active staining of culture broth of transformant grown on starch medium. Enzymatic reaction products with respect to the culture time and the reaction time were examined by TLC analysis. The results indicated that the enzyme activity was exhibited after 12 h cultivation and CD was produced after 10min of enzymatic reaction. When the surface-engineered yeast cells were cultured on galactose medium, maximum activities of CGTase were about 21.3 unit/l and 16.5 unit/l at $25^{\circ}C\;and\;30^{\circ}C$, respectively. The plasmids stability showed about $80\%\;even\;at\;25^{\circ}C\;and\;30^{\circ}C$.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.29 no.5
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• pp.479-485
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• 2011

A number of case studies that find distribution of longevity population and influencing factors through the spatial data fusion using GIS techniques are growing. The majority cases of these studies are adopt census administrative boundary data for the spatial analysis. However, these methods cannot fully explain the phenomenon of longevity because there are a variety of spatial characteristics within the census administrative boundaries. Therefore, studies of spatial unit are required that realistically reflect the phenomenon of human longevity. The dasymetric mapping method enables to product of spatial unit more realistic than census administrative boundary map and statistic estimates of small area utilizing diversity spatial information. In this study, elderly population of small area has been estimated within statistically significant level that applied the vector based dasymetric mapping method. Also, the cluster analysis confirmed that the variation of local spatial relationship within census administrative boundary. The result of this study implied that the need for local-level studies of the human longevity and the validity of the dashmetric mapping techniques.

• Journal of Korea Multimedia Society
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• v.5 no.2
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• pp.158-166
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• 2002

In this paper, we proposed a contour shape description method which use the CFR(contour fluctuation ratio) feature. The CFR is the ratio of the line length to the curve length of a contour segment. The line length means the distance of two end points on a contour segment, and the curve length means the sum of distance of all adjacent two points on a contour segment. We should acquire rotation and scale invariant contour segments because each CFR is computed from contour segments. By using the interleaved contour segment of which length is proportion to the entire contour length and which is generated from all the points on contour, we could acquire rotation and scale invariant contour segments. The CFR can describes the local or global feature of contour shape according to the unit length of contour segment. Therefore we describe the shape of objects with the feature vector which represents the distribution of CFRs, and calculate the similarity by comparing the feature vector of corresponding unit length segments. We implemented the proposed method and experimented with rotated and scaled 165 fish images of fifteen types. The experimental result shows that the proposed method is not only invariant to rotation and scale but also superior to NCCH and TRP method in the clustering power.

• Bulletin of the Korean Space Science Society
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• 2004.10b
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• pp.296-299
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• 2004

KARI precision attitude determination system has been developed for high accurate geo-coding of KOMPSAT-2 image. Sensor data from two star trackers and a IRU are used as measurement and dynamic data. Sensor data from star tracker are composed of QUEST and unit vector filter. Filter algorithms consists of extended Kalman filter, unscented Kalman filter, and least square batch filter. The type of sensor data and filter algorithm can be chosen by user options. Estimated parameters are Euler angle from 12000 frame to optical bench frame, gyro drift rate bias, gyro scale factor, misalignment angle of star tracker coordinate frame with respect to optical bench frame, and misalignment angle of gyro coordinate frame with respect to optical bench frame. In particular, ground control point data can be applied for estimating misalignment angle of star tracker coordinate frame. Through the simulation, KPADS is able to satisfy the KOMPSAT-2 mission requirement in which geo-location accuracy of image is 80 m (CE90) without ground control point.

• KSBB Journal
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• v.24 no.4
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• pp.341-346
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• 2009

Candida Antarctica lipase A (CalA) has been used because of its suitability in industrial applications. CalA has unique features capable to accept tertiary and sterically hindered alcohols among many hydrolases. CalA gene was cloned and constructed in expression vector such as pColdIII/CalA and $pPICZ{\alpha}A$/CalA. The gene encoding pColdIII/CalA was functionally expressed in the cytoplasm of Escherichia coli $Origami^{TM}$ B (DE3) cells. The plasmid $pPICZ{\alpha}A$/CalA linearized by BstX I was integrated into 5'AOX1 region of the chromosomal DNA and was functionally expressed in the methyl atrophic yeast Pichia pastoris. Expressed CalA in P. pastoris (0.7 Unit/mL) showed 35 times higher activity than that in E. coli expression system (0.02 Unit/mL).

• Journal of Mechanical Science and Technology
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• v.20 no.4
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• pp.447-454
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• 2006

Hydraulic actuators are important in modern industry due to high power, fast response, and high stiffness. In recent years, hybrid actuation system, which combines electric and hydraulic technology in a compact unit, can be adapted to a wide variety of force, speed and torque requirements. Moreover, the hybrid actuation system has dealt with the energy consumption and noise problem existed in the conventional hydraulic system. Therefore, hybrid actuator has a wide range of application fields such as plastic injection-molding and metal forming technology, where force or pressure control is the most important technology. In this paper, the solution for force control of hybrid system is presented. However, some limitations still exist such as deterioration of the performance of transient response due to the variable environment stiffness. Therefore, intelligent switching control using Learning Vector Quantization Neural Network (LVQNN) is newly proposed in this paper in order to overcome these limitations. Experiments are carried out to evaluate the effectiveness of the proposed algorithm with large variation of stiffness of external environment. In addition, it is understood that the new system has energy saving effect even though it has almost the same response as that of valve controlled system.