• 제어로봇시스템학회논문지
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• 제7권4호
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• pp.344-350
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• 2001

In this paper, we are focused on the analysis of acyclic schedule for the determination of the opti-mal cycle time and minimization of work in process. Especially, this paper deals with the product ratio-driven cyclic scheduling problem in FMS with different products and ratio using the TPN(Time Petri Nets)un-folding.

• 산업경영시스템학회지
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• 제14권24호
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• pp.179-185
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• 1991

This study aims to consider the necessity and meaning of the Company-Wide Creativity Development(CWCD) for the unfolding of the strategic management in the creation age. The brief summary of the meaning of the CWCD for the unfolding of the strategic management is as follows. (1) The activation of the information interchange through the consonance among the all department. (2) The Information interchange and cooperation between the worker and the manage. (3) The creativity development of worker.

• BMB Reports
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• 제39권1호
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• pp.55-60
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• 2006

We describe a phage display strategy, based on the differential resistance of proteins to denaturant-induced unfolding, that can be used to select protein variants with improved conformational stability. To test the efficiency of this strategy, wild-type and two stable variants of ${\alpha}_1$-antitrypsin (${\alpha}_1AT$) were fused to the gene III protein of M13 phage. These phages were incubated in unfolding solution containing denaturant (urea or guanidinium chloride), and then subjected to an unfavorable refolding procedure (dialysis at $37^{\circ}C$). Once the ${\alpha}_1AT$ moiety of the fusion protein had unfolded in the unfolding solution, in which the denaturant concentration was higher than the unfolding transition midpoint ($C_m$) of the ${\alpha}_1AT$ variant, around 20% of the phage retained binding affinity to anti-${\alpha}_1AT$ antibody due to a low refolding efficiency. Moreover, this affinity reduced to less than 5% when 10 mg/mL skimmed milk (a misfolding-promoting additive) was included during the unfolding/refolding procedure. In contrast, most binding affinity (>95%) remained if the ${\alpha}_1AT$ variant was stable enough to resist unfolding. Because this selection procedure does not affect the infectivity of M13, the method is expected to be generally applicable to the high-throughput screening of stable protein variants, when activity-based screening is not possible.

• 한국멀티미디어학회논문지
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• 제9권9호
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• pp.1160-1172
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• 2006

펼친 영상 생성 기법은 대장 전체를 하나의 영상으로 보여주므로 병변을 식별하는데 효과적이다. 그러나 광선 투사법에 기반한 이전의 펼친 영상 생성 방법은 대장의 굴곡이 심한 부분이나 접힌 부분에서 숨겨진 병리구조는 제대로 표현할 수 없었다. 이 문제를 해결하기 위해, 본 논문에서는 표면 복잡도에 따라 투사되는 광선 수를 가변적으로 조정하는 방법을 제안한다. 첫 번째로 중심 경로상의 샘플점에서 대장 벽까지 이르는 거리 값이 큰 샘플점과 작은 샘플점들을 선택하여 그 점을 기준이 되는 제어점으로 결정한다. 다음으로 샘플점에서 발사된 광선이 주름진 영역 안쪽까지 도달하도록 하기 위해 제어점 사이에 있는 샘플점들을 거리 값이 큰 제어점 부근으로 이동시킨다. 마지막으로 영상을 펼쳤을 때 일어나는 왜곡 현상을 줄이기 위해 이차원 크기변환을 한다. 이 방법을 이용하면 대장 조직을 정확하게 표현할 수 있다.

• BMB Reports
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• 제35권2호
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• pp.143-154
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• 2002

The structural and functional aspects of ervatamin B were studied in solution. Ervatamin B belongs to the $\alpha+\beta$ class of proteins. The intrinsic fluorescence emission maximum of the enzyme was at 350 nm under neutral conditions, and at 355 nm under denaturing conditions. Between pH 1.0-2.5 the enzyme exists in a partially unfolded state with minimum or no tertiary structure, and no proteolytic activity. At still lower pH, the enzyme regains substantial secondary structure, which is predominantly $\beta$-sheet conformation and shows a strong binding to 8-anilino-1-napthalene-sulfonic acid (ANS). In the presence of salt, the enzyme attains a similar state directly from the native state. Under neutral conditions, the enzyme was stable in urea, while the guanidine hydrochloride (GuHCl) induced equilibrium unfolding was cooperative. The GuHCl induced unfolding transition curves at pH 3.0 and 4.0 were non-coincidental, indicating the presence of intermediates in the unfolding pathway. This was substantiated by strong ANS binding that was observed at low concentrations of GuHCl at both pH 3.0 and 4.0. The urea induced transition curves at pH 3.0 were, however, coincidental, but non-cooperative. This indicates that the different structural units of the enzyme unfold in steps through intermediates. This observation is further supported by two emission maxima in ANS binding assay during urea denaturation. Hence, denaturant induced equilibrium unfolding pathway of ervatamin B, which differs from the acid induced unfolding pathway, is not a simple two-state transition but involves intermediates which probably accumulate at different stages of protein folding and hence adds a new dimension to the unfolding pathway of plant proteases of the papain superfamily.

• 한국항공우주학회지
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• 제37권3호
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• pp.283-292
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• 2009

공력면의 전개 거동을 지배하는 미분방정식을 적분 형태를 바꾸어 전개 가능한 조건을 유도하여 전개에 필요한 힌지 토크를 추산할 수 있는 방정식을 얻었다. 방정식의 해는 주어진 전개 조건과 공력 조건에 대해 전개가 가능하기 위하여 전개 장치에 저장되어야 할 최소의 전개 에너지이며 방정식을 풀기위하여 공력과 중력, 마찰력 등 공력면에 작용하는 모멘트의 적분을 계산하여야 한다. 공력 롤 감쇠와 마찰력과 같은 비가역적 현상에 대한 적분을 수행하기 위하여 몇가지 가정과 모델링이 필요하였으며 특히 공력 롤 감쇠의 적분을 위하여 전개 각속도를 전개각만의 함수로 표현되도록 모델링하였고 이는 전개 과정에서 나타날 수 있는 최대 각속도와 형태를 가정함으로써 가능하였다. 방정식 풀이와 전개 각속도의 모델을 위하여 전개 에너지를 대표하는 이상 전개 각속도를 정의하였으며 최소의 전개 에너지를 구하는 방정식은 이상 전개 각속도에 대한 이차 방정식으로 표현된다. 제안된 추산법을 검증하기 위하여 여러 가지 전개 조건과 공력 조건에 대하여 최소 전개에너지를 추산하고 기 개발된 전개 거동 모사 프로그램을 이용하여 여러 형태의 전개 토크 형태에 대하여 추산 결과의 타당성을 비교 검토하였다.

• Nuclear Engineering and Technology
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• 제55권3호
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• pp.1021-1030
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• 2023

We propose an overall procedure for measuring and unfolding fast neutron spectra using a trans-stilbene scintillation detector. Detector characterization was described, including the information on energy calibration, detector resolution, and nonproportionality response. The digital charge comparison method was used for the investigation of neutron-gamma Pulse Shape Discrimination (PSD). A pair of values of 600 ns pulse width and 24 ns delay time was found as the optimized conditions for PSD. A fitting technique was introduced to increase the trans-stilbene Proton Response Function (PRF) by 28% based on comparison of the simulated and experimental electron-equivalent distributions by the Cf-252 source. The detector response matrix was constructed by Monte-Carlo simulation and the spectrum unfolding was implemented using the iterative Bayesian method. The unfolding of simulated and measured spectra of Cf-252 and AmBe neutron sources indicates reliable, stable and no-bias results. The unfolding technique was also validated by the measured cosmic-ray induced neutron flux. Our approach is promising for fast neutron detection and spectroscopy.

• 한국실내디자인학회논문집
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• 제21권4호
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• pp.68-76
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• 2012

Contemporary architecture has a tendency to concretely approach based on the concept of Deuleuze's 'fold' diversity problems creating continuously potential diversities within Post-structural paradigm. Contemporary architecture relates to human life through its relation with human being. The research investigates through case study the possible relation of the concept of Deulezue's 'fold', one of his concepts of 'unfolding' based on Monadology, with logic of creation that constructs a space in contemporary architectural space. Eight contemporary architectural spaces since 1990 with architectural embodiment of Deulezue's 'fold' concept have been selected as cases. Based on the fact that the potential possibility of a space can be embodied by the thinking of 'fold', the research draws four physical activities such as 'properties connection', 'beyond border', 'diversity of circulation' and 'potential opportunity', through which it studies on the principle of actualization of space creation. Consideration of metaphysical theory as a pre-modern and outdated one may lose a huge potentiality. Leibniz's metaphysics can be rediscovered as a model that provides diverse potentiality. The concept of 'fold' in Monadology needs be reconsidered because it possibly suggests a methodology that embodies through the actualization of space the meanings underlain in contemporary architectural space.

• BMB Reports
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• 제40권2호
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• pp.205-211
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• 2007

The stability curve - a plot of the Gibbs free energy of unfolding versus temperature - is calculated for bovine erythrocyte carbonic anhydrase in 150 mM sodium phosphate (pH = 7.0) from a combination of reversible differential scanning calorimetry measurements and isothermal guanidine hydrochloride titrations. The enzyme possesses two stable folded conformers with the conformational transition occurring at ~30$^{\circ}C$. The methodology yields a stability curve for the complete unfolding of the enzyme below this temperature but only the partial unfolding, to the molten globule state, above it. The transition state thermodynamics for the low- to physiological-temperature conformational change are calculated from slow-scan-rate differential scanning calorimetry measurements where it is found that the free energy barrier for the conversion is 90 kJ/mole and the transition state possesses a substantial unfolding quality. The data therefore suggest that the x-ray structure may differ considerably from the physiological structure and that the two conformers are not readily interconverted.

• 한국항공우주학회지
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• 제36권5호
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• pp.420-427
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• 2008

접힌 공력면이 전개하는 운동을 모사하기 위하여 전개 운동 방정식을 수립하고 공력면에 작용하는 모멘트 등을 모델링하였다. 공력에 의한 롤 모멘트는 정적 롤 모멘트와 감쇠 모멘트로 이루어져 있으며 정적 롤 모멘트는 전개 하중 측정을 위한 풍동 시험을 통하여 획득한 정적 롤 모멘트 계수로부터 계산된다. 롤 감쇠 계수는 패널법을 이용한 수치 해석을 통하여 산출되었는데 전개 각속도로부터 유발된 받음각에 상응하는 변위각을 공력면에 적용하여 수치해석을 하였다. 공력면 형상에 적용한 변위각은 회전 중심으로부터 공력면 끝단까지 선형적으로 증가하며 회전 중심에서는 변위가 없다. 롤 감쇠 계수는 전개 각속도에 따른 롤 모멘트 계수의 변화율로 계산되며 공력조건과 전개각의 함수이고 전개 각속도에 대해서는 일정하다고 가정하였다. 롤 감쇠를 포함하여 모사된 공력면의 전개 모사는 시험과 비교하여 유사한 결과를 보여주었다.