• Journal of Pharmaceutical Investigation
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• 제30권1호
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• pp.51-54
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• 2000

A column-switching high performance liquid chromatographic method has been developed for the determination of a fluconazole in human plasma. Each plasma sample was centrifuged for 10 min at 5000 g. After an aliqout of the supernatant was taken to nylon microcentrifuge filter, these samples were centrifuged for 10 min at 5000 g. An aliqout of the supernatant was injected directly onto the HPLC column. Deionized water was run for 2 min at a flow rate of 1.0 ml/min to retain fluconazole in an extration column, while proteins and endogenous interferences were eluted to the waste. The analyte was then back-flushed onto an analytical column, $C_{18}$ reversed-phase column. The mobile phase for analytical column, 0.01 M sodium acetate (pH 5.0)-methanol (65:35, v/v), was run at a flow rate of 1.0 ml/min. The column effluent was monitored by ultraviolet detection at 261 nm. The retention time for fluconazole was 11.76 min in human plasma. The detection limit for fluconazole in human plasma was $0.2\;{\mu}g/ml$. No interference from endogenous substances was observed.

• 생약학회지
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• 제48권1호
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• pp.93-96
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• 2017

Ephedra alkaloids, (-)-ephedrine, (+)-pseudoephedrine, (-)-N-methylephedrine, (+)-N-methylpseudoephedrine, (-)-norephedrine and (+)-norpseudoephedrine, from ephedra herb are sympathomimetic agonists causing an increase of metabolism, blood pressure and perspiration. In this study, we developed the validation method of (+)-pseudoephedrine and (-)-ephedrine, two major ephedra alkaloids in Ephedra spp., by high-performance liquid chromatography-ultraviolet spectrometer (HPLC-UV). HPLC analysis was performed using a HECTOR-M C18 column operating at $35^{\circ}C$, and UV detection at 215nm. The mobile phase used a gradient flow with 25 mM SDS in water (A) and acetonitrile (B).

• 조명전기설비학회논문지
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• 제28권3호
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• pp.78-83
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• 2014

To minimize the financial loss due to power facility malfunction, on-line diagnostic techniques are required to grasp any abnormal state of facilities in the live line as well as devices to diagnose abnormal states of power facility in an easy and prompt manner. This study aims to develop a portable UV detecting system by means of UV sensors for easier and efficient inspection of the degradation state of power facility in a long distance. Accordingly, it includes a simulation of corona discharges that may occur due to degradation of power facility and detection of ultraviolet pulse generation depending on the corona discharge intensity and measuring distance in application of UV sensors. Additionally, the optimal algorithm is determined for its application to the system's degradation diagnosis program based on the measured experiment data.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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• pp.295-295
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• 2000

In this paper, the measurement algorithm of PCB alignment error is developed using image processing. The X-Y-$\theta$ table correcting PCB alignment error is driven by drive module based on microprocessor according to calculation results obtained through image processing procedure. In order to recognize the X-Y-$\theta$ position errors, two fiducial points are marked on PCB and two cameras of ultraviolet rays types are used for detection of the points to capture exactly fiducial points under disturbance of illumination change. Through application for a practical screen printer, the precision control using the developed position control system can be realized about 2.5${\mu}{\textrm}{m}$ in table moving range and 8${\mu}{\textrm}{m}$ in camera processing precision.

• 세라미스트
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• 제22권1호
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• pp.27-35
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• 2019

Research on ultraviolet (UV) light detection has attracted considerable attention from scientific researchers in related fields. It can be said that it is a very important time to accurately monitor the UV irradiation amount according to the wavelength region in real time. The oxide is very diverse in its kind and has the advantage of being able to efficiently control the band gap through band gap engineering. In addition, it is very stable in response to heat and atmospheric oxygen when UV is absorbed. Also, there is a known method that can effectively manufacture oxide nanoparticles and nanorods through various synthesis methods, and researches for improving the sensitivity of UV sensors have been carried out using this method. In this paper, we introduce the materials that can be used as UV sensors among various wide band oxide materials, and review the results of researches of various UV sensors using nano materials.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2009년도 춘계학술대회 논문집
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• pp.290-293
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• 2009

This paper presents the development of a disposable label-free biosensor for bio molecular interaction analysis. Label-free biosensors have advantages of high performance in sensitivity and short detection time. Among various label-free systems, we introduced biosensor with nano grating structures based on white light source and spectrometer. And to develop high efficiency label-free biosensor, we suggest replicating processes satisfying required specification. We also report a system set-up to evaluate the characteristics of phenomenon shown in this biosensor system.

• 한국환경농학회지
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• 제23권4호
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• pp.245-250
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• 2004

An analytical method was developed to determine monocrotophos residues in apple, citrus, and soil using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. Monocrotophos was extracted with acetone from apple, citrus and moist soil samples. The extract was concentrated, added with saline water, and subjected to n-hexane washing to remove nonpolar co-extractives. Dichloromethane partition was then followed to recover monocrotophos from the aqueous phase. Silica gel column chromatography was employed to further purify the extract prior to HPLC determination. Reverse-phase HPLC using an oct-adecylsilyl column was successfully applied to separate and quantitate the monocrotophos residue in sample extracts at the wavelength of 230 nm. Overall recoveries of monocrotophos from fortified samples averaged $95.3{\pm}2.1%$ (n=6), $970{\pm}0.7%$ (n=6), and $92.8{\pm}4.3%$ (n=12) for apple, citrus, and soil, respectively. The proposed method was quite reproducible and sensitive enough to replace the troublesome gas-liquid chromatographic analysis for monocrotophos residues.

• 전기학회논문지P
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• 제57권4호
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• pp.417-423
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• 2008

For the evaluation in the safety of outdoor insulator, there are various methods such as: visual observation; voltage measurement; use of ultrasonic wave due to partial discharge; electric field measurement; use of infrared/ultraviolet sensor-type camera. However, many problems have been issued regrading the reliability of the evaluation test and safety of testing operation. Accordingly, the present study has investigated and analyzed the characteristics and mechanisms of the insulator deterioration, in order to obtain an optimal method for detecting poor insulator so that the reliability of power facilities can be enhanced. Besides, based on theoretical analysis of domestic and international detection methods for poor insulator, the reliability has been compared and evaluated quantitatively. As a result, it was shown that the corona camera would be the best choice for domestic workplace application in detecting poor insulator for enhancing the reliability of power transmission line.

• Nuclear Engineering and Technology
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• 제50권5호
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• pp.751-757
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• 2018

In this article, a UV sensor that is an appropriate tool for fire detection has been designed and constructed. The structure of this UV sensor is an air-filled single-wire detector that is able to operate under normal air condition. A reflective CsI photocathode is installed at the end of the sensor chamber to generate photoelectrons in the ion chamber. An electric current is produced by accelerating photoelectrons to the anode in the electric field. The detector is able to measure the intensity of the incident UV rays whenever the current is sufficiently high. Therefore, the sensitivity coefficient of this sensor is found to be $7.67{\times}10^{-6}V/photons/sec$.

• Journal of Photoscience
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• 제9권3호
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• pp.33-36
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• 2002

This study describes RT-PCR and in situ hybridisation protocols, and the immunohistochemical detection method that we have developed to detect and localise cells that express HO-1 in the skin. We found that HO-1 mRNA was absent in normal mouse skin, but after UVA irradiation HO-1 mRNA was expressed in the dermal fibroblasts, and strongly in basal epidermal cells. HO-1 protein was also induced strongly in dermal fibroblasts, and also in epidermal cells. In addition, the HO substrate heme was reduced in skin microsome at 72 hrs post UVA (when HO activity is high). At the same time, the HO products bilirubin and iron levels were elevated in the cutaneous tissue. Thus in addition to a dermal response, there appears to be an epidermal HO response to UVA in vivo that may be relevant for immune modulation by UVA radiation.