Journal of Pharmaceutical Investigation
- Volume 30 Issue 1
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- Pages.51-54
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- 2000
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- 2093-5552(pISSN)
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- 2093-6214(eISSN)
Column-switching High Performance Liquid Chromatographic Determination of Fluconazole in Human Plasma
컬럼 스위칭 고속액체크로마토그라프법을 이용한 혈장 중 플루코나졸의 분석
- Jee, Jun-Pil (College of Pharmacy, Seoul National University) ;
- Jin, Sook (College of Pharmacy, Seoul National University) ;
- Lee, Mi-Kyung (College of Pharmacy, Seoul National University) ;
- Kim, Yang-Bae (College of Pharmacy, Seoul National University) ;
- Kim, Chong-Kook (College of Pharmacy, Seoul National University)
- Published : 2000.03.20
Abstract
A column-switching high performance liquid chromatographic method has been developed for the determination of a fluconazole in human plasma. Each plasma sample was centrifuged for 10 min at 5000 g. After an aliqout of the supernatant was taken to nylon microcentrifuge filter, these samples were centrifuged for 10 min at 5000 g. An aliqout of the supernatant was injected directly onto the HPLC column. Deionized water was run for 2 min at a flow rate of 1.0 ml/min to retain fluconazole in an extration column, while proteins and endogenous interferences were eluted to the waste. The analyte was then back-flushed onto an analytical column,