Browse > Article

Simultaneous Determination of (+)-Pseudoephedrine and (-)-Ephedrine in Ephedra intermedia by HPLC-UV  

Jeong, Birang (College of Pharmacy, Kangwon National University)
Yoon, Yoosik (College of Medicine, Chung-Ang University)
Shin, Soon Shik (College of Korean Medicine, Dong-eui University)
Kwon, Yong Soo (College of Pharmacy, Kangwon National University)
Yang, Heejung (College of Pharmacy, Kangwon National University)
Publication Information
Korean Journal of Pharmacognosy / v.48, no.1, 2017 , pp. 93-96 More about this Journal
Abstract
Ephedra alkaloids, (-)-ephedrine, (+)-pseudoephedrine, (-)-N-methylephedrine, (+)-N-methylpseudoephedrine, (-)-norephedrine and (+)-norpseudoephedrine, from ephedra herb are sympathomimetic agonists causing an increase of metabolism, blood pressure and perspiration. In this study, we developed the validation method of (+)-pseudoephedrine and (-)-ephedrine, two major ephedra alkaloids in Ephedra spp., by high-performance liquid chromatography-ultraviolet spectrometer (HPLC-UV). HPLC analysis was performed using a HECTOR-M C18 column operating at $35^{\circ}C$, and UV detection at 215nm. The mobile phase used a gradient flow with 25 mM SDS in water (A) and acetonitrile (B).
Keywords
HPLC analysis; Ephedra intermedia; Ephedrine; Pseudoephedrine; Method validation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 식품의약품안전처 (2014) 대한약전 제11개정, 식품의약품안전처, 서울
2 White, L. M., Gardner, S. F., Gurley, B. J., Marx, M. A., Wang, P. L. and Estes, M. (1997) Pharmacokinetics and cardiovascular effects of ma-huang(Ephedra sinica) in normotensive adults. J. Clin. Pharmacol 22: 116-122.
3 Yamada, I., Goto, T., Takeuchi, S., Ohshima, S., Yoneyama, K., Shibuya, T., Kataoka, E., Segawa, D., Sato, W., Dohmen, T, Anezaki, Y., Ishii, H. and Ohnishi, H. (2007) Mao(Ephedra sinica Stapf) protects against D-galactosamine and lipopolysaccharide-induced hepatic failure. Cykokine 41: 293-301.
4 Pellati, F. and Benvenuti, S. (2000) Determination of ephedrine alkaloids in Ephedra natural products using HPLC on a pentafluorophenylpropyl stationary phase. J. Pharm. Biomed. Anal. 48: 254-263.