• Title/Summary/Keyword: Ultrasonication

Search Result 188, Processing Time 0.025 seconds

Effects of ultrasonication on the analysis of sulforaphane content in vegetables (초음파 처리가 채소 중의 sulforaphane 분석에 미치는 영향)

  • Choi, A-Reum;Lee, Gun-Soon;Chae, Hee-Jeong
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.9 no.3
    • /
    • pp.794-799
    • /
    • 2008
  • The effect of ultrasonication on the sulforaphane analysis in cruciferous vegetables was investigated by GC/MS. The ultrasonication of the analysis samples was carried out in dichloromethane as a solvent, which was followed by concentration in nitrogen gas, and the analysis of sulforaphane was performed using selective ion monitoring (SIM) at m/z 72, 160, 55 and 114. The content of sulforaphane was the highest in the extract of broccoli (149 ppm), and followed by cabbage (67.9 ppm) and radish (35.4 ppm). When the vegetable samples were shaked after ultrasonication, the extraction efficiency of sulforaphane was 2.7-fold enhanced, compared to the extraction by shaking. The result suggested that pretreatment including sonication can be used fer improving the extraction efficiency of sulforaphane.

Effects of ultrasonication intensity and shaking time on the rheological behavior of alumina slurries with maltodextrin (말토 덱스트린 첨가 알루미나 슬러리의 유동특성에 미치는 초음파 처리 강도와 진동 시간의 영향 검토)

  • 김종철;오근호
    • Journal of the Korean Crystal Growth and Crystal Technology
    • /
    • v.10 no.1
    • /
    • pp.80-85
    • /
    • 2000
  • The rheology of alumina slurries with maltodextrin was studied with different processing routes using experimental design and statistical analysis. Different processing routes include maltodextrin addition, different ultrasonication intensity applied to the slurries before or after adding maltodextrin, and shaking time. Viscosities of the slurries showed shear thinning behavior and were correlated with the Ostwald-de-Weale model. The viscosities of alumina slurries decreased with the addition of maltodextrin and increased with ultrasonication intensity. There were little differences in the viscosities of the slurries depending on whether maltodextrin was added before or after ultrasonication.

  • PDF

Seed-dependent Accelerated Fibrillation of ${\alpha}$-Synuclein Induced by Periodic Ultrasonication Treatment

  • Kim, Hyun-Jin;Chatani, Eri;Goto, Yuji;Paik, Seung-R.
    • Journal of Microbiology and Biotechnology
    • /
    • v.17 no.12
    • /
    • pp.2027-2032
    • /
    • 2007
  • [ ${\alpha}$ ]-Synuclein is the major component of Lewy bodies and responsible for the amyloid deposits observed in Parkinson's disease. Ordered filamentous aggregate formation of the natively unfolded ${\alpha}$-synuclein was investigated in vitro with the periodic ultrasonication. The ultrasonication induced the fibrillation of ${\alpha}$-synuclein, as the random structure gradually converted into a ${\beta}$-sheet structure. The resulting fibrils obtained at the stationary phase appeared heterogeneous in their size distribution, with the average length and height of $0.28\;{\mu}m{\pm}0.21\;{\mu}m$ and $5.6\;nm{\pm}1.9\;nm$, respectively. After additional extensive ultrasonication in the absence of monomeric ${\alpha}$-synuclein, the equilibrium between the fibril formation and its breakdown shifted to the disintegration of the preexisting fibrils. The resulting fragments served as nucleation centers for the subsequent seed-dependent accelerated fibrillation under a quiescent incubation condition. This self-seeding amplification process depended on the seed formation and subsequent alterations in their properties by the ultrasonication to a state that accretes the monomeric soluble protein more effectively than their reassociation of the seeds back to the original fibrils. Since many neurodegenerative disorders have been considered to be propagated via the seed-dependent amyloidosis, this study would provide a novel aspect of the significance of the seed structure and its properties leading to the acce]erated amyloid formation.

Changes in Ginsenosides Composition of Ginseng Leaf and Stem after an Ultrasonication Process (초음파 처리에 의한 인삼 잎과 줄기의 진세노사이드 성분 변화)

  • Nam, Yun Min;Shin, Ho June;Yang, Byung Wook;Park, Jong Dae;Cho, Soon Hyun;Kim, Hyoung Chun;Ko, Sung Kwon
    • Korean Journal of Pharmacognosy
    • /
    • v.47 no.4
    • /
    • pp.352-359
    • /
    • 2016
  • The purpose of this study is to develop a new preparation process of ginseng leaf and stem extracts having high concentrations of ginsenoside Rg2, Rg3, Rg5, Rh1, a special component of red and black ginseng. Chemical transformation from ginseng saponin glycosides to prosapogenin was analyzed by the HPLC. Extracts of ginseng (Panax ginseng) leaf and stem were processed under several treatment conditions including ultrasonication treatments. The content of total saponin reached their heights at 17 hr (UGL-17) of ultrasonication treatment, followed by 16 hr (UGL-16) and 7 hr (UGL-7) of ultrasonication treatment at $100^{\circ}C$. UGL-17 findings show that the ginseng leaf and stem that had been processed with ultrasonication for 17 hours peaked in the level of Rg2, Rg3 and Rh1. In addition, UGL-16 contained ginsenoside Rg5 at high concentrations. It is thought that such results provide basic information in preparing ginseng leaf and stem extracts with functionality enhanced.

The Effects of ultrasonic irradition on germination of mung bean (녹두 발아에 미치는 초음파 조사의 영향)

  • Koh, Moo-Seok;Lee, Y.B.
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.18 no.2
    • /
    • pp.153-159
    • /
    • 1989
  • The experiment was carried out to examine on effect of ultrasonication on the growith of a mungbean while a mungbean is germinated and to measure amino acid content of a mungbean during its germination. Mungbean seeds soaked are divided into three groups; the first group was control group, untreated and naturally germinated. the second was ultrasonic treated group(UTG I), irradiated by ultrasonication for 8, 16 and 24min respectively immediately after soaking. the third was ultrasonic treated group(UTG II), irradiated by ultrasonication after 48hr germinating. The results were as foolows; Fresh weight, moisture, length of mungbean increased as its germination proceeds. while dry weight decreased. Ultrasonication tends to inhibit its germination of mungbean and results in the decrease in weight, length and moisture. Specially ultrasonication had a marked effect on UTG II. The longer the time of ultrasonication was, the more was the growth inhibited. In the control groups, the content of amino acid increased until 72 hours, but after that, it decreased. of the amino acid determined, glutamic acid decreased marekedly in the cotyledons, While the opposite was the case with aspartic acid in the hypocotyls. Comparing with the control groups, UTG I, the content of amino acid decreased. As the ultrasonic treaed time was increased, the concentration of amino acid in hypocotyl cotyledon tended to decreased. UTG II, the content of amino acid was lower than control groups, but higher than UTG I, and the effects of ultrasonication were similar to UTG I.

  • PDF

A comparative study on applicability of nano-sized iron(II, III) oxide in ultrasonicated Fenton process

  • Sahinkaya, Serkan;Yakut, Sennur Merve
    • Environmental Engineering Research
    • /
    • v.25 no.1
    • /
    • pp.36-42
    • /
    • 2020
  • Fenton process is one of the most effective advanced oxidation processes for the removal of pollutants from wastewater. In this study, while ferrous iron was used in conventional Fenton process (CFP); nano-sized iron(II, III) oxide was experienced in modified Fenton process (MFP) as a new catalyst alternative. In order to enhance their oxidation efficiencies, both CFP and MFP were combined with ultrasonication at 53 kHz fixed frequency. Thus, the influences of both catalyst iron species and ultrasonication on color and chemical oxygen demand (COD) removals from synthetic textile wastewater including Maxilon Red GRL 200% dyestuff were investigated experimentally. While the COD and color removal rates were found as 72.5% and 69.7% via CFP; they were 87% and 75.8% by ultrasonicated CFP, respectively. The color and COD removals were 40.6% and 64.8% via MFP, and 49.9 and 73.1% by ultrasonicated MFP, respectively. Therefore, it was found that the simultaneously usage of ultrasonication with CFP and MFP was improved the COD and color removal efficiencies and oxidation rates even at lower H2O2 dosages, compared to individual CFP and MFP. Moreover, the color and COD removal kinetics were also modelled mathematically and compared in the study.

Chemical coagulation and sonolysis for total aromatic amines removal from anaerobically pre-treated textile wastewater: A comparative study

  • Verma, Akshaya K.;Bhunia, Puspendu;Dash, Rajesh R.
    • Advances in environmental research
    • /
    • v.3 no.4
    • /
    • pp.293-306
    • /
    • 2014
  • The present study primarily focuses on the evaluation of the comparative effect of chemical coagulation and ultrasonication for elimination of aromatic amines (AAs) present in anaerobically pretreated textile wastewater containing different types of dyes including azo dyes. Color and COD reduction was also monitored at the optimized conditions. The production of AAs was measured spectrophotometrically in the form of total aromatic amines (TAAs) and also verified with high performance liquid chromatography (HPLC) selectively. A composite coagulant, magnesium chloride (MC) aided with aluminium chlorohydrate (ACH) in an equal ratio (MC + ACH) was utilized during the coagulation process, which yielded 31% of TAAs removal along with 85% of color and 52% of COD reduction. At optimized power (200 W) and sonication time (5 h), an appreciable TAAs degradation efficiency (85%) was observed along with 51% color reduction and 62% COD removal using ultrasonication. The chromatographic data indicate that sulphanilic acid and benzidine types of aromatic amines were produced after the reductive cleavage of utilized textile dyes, which were effectively mineralized after ultrasonication. The degradation followed the first order kinetics with a correlation coefficient ($R^2$) of 0.89 and a first-order kinetic constant (k) of $0.0073min^{-1}$.

Effect of ultrasonication, light and liquid smoke treatment on germination of lettuce seeds

  • Park, Sunyeob;Kim, Young Ae;Kim, Min Geun;Kim, Du Hyun
    • Proceedings of the Korean Society of Crop Science Conference
    • /
    • 2017.06a
    • /
    • pp.217-217
    • /
    • 2017
  • Seed priming leading to improved emergences, germination speed and uniformity under unfavorable conditions without loss of longevity are the best level of techniques. We studied the possibility to obtain primed seed with reduced mean germination time (MGT) and uniformity but with simple, fast and effective methods. The experiments were carried out at $15^{\circ}C$ for 16 hours with priming treatment. To compare the effects of each treatment, we used 200mM $CaCl_2$ priming or hydro priming or no imbibition after ultrasonication or red light treatment. The light treatment was performed by irradiating 2000 lux of red light for 15, 30, 60, and 120 minutes. Addition, in order to investigate the effect of the priming solution, treated at 200mM $CaCl_2$ for 8 hours. Ultrasonication treatment was performed for 5, 10, and 20 minutes at exposures of 13.0, 17.3, and 21.6 kHz during priming. For liquid smoke (LS) treatment, seed soaked in 0%, 0.5%, 1.0%, 5.0% and 10.0% of diluted water and 200mM $CaCl_2$ solution. After each treatment, the seeds were dried to moisture content ranged 5-8% at $25^{\circ}C$ for 24 hours. The effect of seed treatment was evaluated with germination percentage (GP), MGT, germination index(GI), germination rate(GR), Germination Uniformity(GU) and heath seed percentage(HS). For several factors tested, we found that the desired germination improvement could be obtained by treating the seed with ultrasonication at 17.3 kHz for 5 minutes in water or red light exposure at 2000 lux for 120 min in water that resulted very similar to those used to 200mM $CaCl_2$ priming for 16 hrs. However, LS treatment showed no improvement in all diluted solution. Therefore, the methods applied ultrasonication and red light treatment showed high potential for fast and easy treatment avoiding pollution of salt solution waste.

  • PDF

Acid Fermentation Characteristics of Waste Activated Sludge using Acids and Ultrasonication (산용해 및 초음파를 이용한 하수 슬러지의 산발효 특성)

  • Sohn, C.H.;Hong, S.M.;Lee, B.H.
    • Journal of Korean Society on Water Environment
    • /
    • v.23 no.5
    • /
    • pp.781-788
    • /
    • 2007
  • The Carbon source to enhance the denitrification is essential matter in the advanced sewage treatment. For the high level of nutrient removal, external carbons such as ethanol, methanol, volatile fatty acids and so on should be needed. In this study, the methods to increase the sludge solubilization and acidification rate were compared with waste activated sludges and food waste leachate. Ultrasonication and acids were used for the pretreatment of organic particles in sludges. As a results, the optimal temperature and HRT were $60^{\circ}C$ and 5 days, respectively. HAc, HPr, HBr, and other VFAs for acid fermentations reduced up to 22, 16, 14, and 48% with HRT reduction. For the increase of solubilization, 28% of solids destruction rate was shown at 0.3 watts/mL.