• Title/Summary/Keyword: UV-B조사

Search Result 221, Processing Time 0.029 seconds

Photosynthesis and Formation of UV-absorbing Substances in Antarctic Macroalgae Under Different Levels of UV-B Radiation (중파자외선에 대한 남극산 해조류의 광합성 및 자외선 흡수물질 형성 반응)

  • 한태준;박병직;한영석;강성호;이상훈
    • Korean Journal of Environmental Biology
    • /
    • v.20 no.3
    • /
    • pp.205-215
    • /
    • 2002
  • Effects of artificial and solar W-B radiation on five rhodophytes (Curdiea racovitzae, Gigaytina skottsbergii, Mazzaella obovata, Myriogramme manginii, Palmaria decipiens) from Antarctica have been investigated using PAM fluorescence in laboratory and in the field. Laboratory studies showed that there were significant differences in the UV sensitivity between different species, and that the differences appeared to be correlated with the depth of collection of the specimens. It was apparent from the observations that the samples such as M. manginii and P. decipiens collected from 20-30 m depths were move sensitive to W-B radiation compared with those collected from shallower depths, The present study confirmed that an acclimation to the surrounding light regime could be an important factor to determine the UV-sensitivity of a species or individuals and that PAM measurements are rapid and non-destructive methods to evaluate UV influences. From field studies on M. manginii and P. decipiens it was observed that both plants exhibited changes in the effective quantum yield, with the minimum values nt noon followed by n recovery in the evening. Photoinhibition occurred in these species could therefore be accounted for by so- called dynamic photoinhibition. It seems likely that this protective mechanism may contribute to survival of the species in shallow water where they may encounter intense solar radiation. The presence or absence of the W- B component under solar radiation differently affected the photosynthetic recovery process, and the rate of recovery was much stoney in UV- present than in W- absent conditions. Functional role of W- B appears to delay the recovery of photosynthesis in the studied macroalgae. Differential sensitivity to UV-B recognised between M. manginii and P. decipiens seemed to correspond well with the amount of UV-absorbing substances (UVAS) contained in the respective species. Higher tolerance to solar radiation by the latter species may be due to the higher amount of UVAS. There were variations of UVAS concentrations in algal thalli depending on the season and depth of collection.

Photoalteration in Biodegradability and Chemical Compositions of Algae- derived Dissolved Organic Matter (자외선에 의한 조류기원 용존유기물의 생분해도 및 화학조성변환.)

  • Imai, Akio;Matsushige, Kazuo;Nagai, Takashi;Kim, Yong-Hwan;Kim, Bom-Chul;Choi, Kwang-Soon
    • Korean Journal of Ecology and Environment
    • /
    • v.36 no.3 s.104
    • /
    • pp.235-241
    • /
    • 2003
  • The effect of ultraviolet (UV) radiation on the characteristics of algae-derived dissolved organic matter (DOM) was examined by comparing the biodegradability and DOM fraction distribution of algal DOM before and after UV exposure. Algal DOM from two axenic cultures of Microcystis aeruginosa and Oscillatoria agardhii were irradiated for 24 h at a UV intensity of 42 W/$m^2$. A complete degradation of algal DOM during the UV exposure did not occur, remaining at constant concentrations of dissolved organic carbon(DOC). After UV exposure, however, microbial degradations were reduced by 17% in M. aeruginosa and 53% in O. agardhii, respectively, and decomposition rates also were two times lower in UV exposed algal DOM. In addition, the chemical compositions of algal DOM altered substantially after UV radiation exposure. The proportions of hydrophilic bases (HiB; protein-like DOM) decreased considerably in both algal DOM sources after UV exposure (16.8% and 20.0% of DOM, respectively), whereas those of hydrophilic acids (HiA; carboxylic acids-like DOM) increased as much as the decrease of the HiB fraction. Capillary ion electrophoresis (CE) analysis showed that several carboxylic acids increased significantly after UV exposure, further confirming an increase in HiA fractions. The results of this study clearly indicate that algal DOM can be changed in its chemical composition as well as biodegradability without complete degradation by UV radiation.

pH Dependence on the Degradation of Rhodamine B by Fe-ACF/$TiO_2$ Composites and Effect of Different Fe Precursors (Fe-ACF/$TiO_2$ 복합체에 의한 로다민 B 용액의 분해에 있어서 pH 의존성 및 여러 가지 Fe 전구체의 효과)

  • Zhang, Kan;Oh, Won-Chun
    • Elastomers and Composites
    • /
    • v.44 no.4
    • /
    • pp.408-415
    • /
    • 2009
  • Iron-loaded activated carbon fibers (Fe-ACF) supported titanium dioxide ($TiO_2$) photocatalyst (Fe-ACF/$TiO_2$) was synthesized using a sol-gel method. Three different types of Fe-ACF/$TiO_2$ were obtained by treatment with different precursor of Fe, and characterized using BET, SEM, XRD and EDX analysis. The photocatalytic activity of Fe-ACF/$TiO_2$ was investigated by the degradation of Rhodamine B (Rh.B) solution under UV irradiation. From the experimental results, it was revealed that Fe-ACF/$TiO_2$ composites show considerable photocatalytic ability for the removal of Rh.B by comparing non-treated ACF/$TiO_2$ composites. And photo-Fenton reaction with Fe element was incoordinately influenced due to different precursor of Fe. It clearly indicates that Fe-ACF/$TiO_2$ composites prepared using $FeCl_3$ provided the highest photo-Fenton activity, then, which was affected by pH changes on the degradation of Rh.B.

Isolation of Chlorella vulgaris Mutants Producing High Lipid and their Characterization (지질 고 생산성 Chlorella vulgaris 변이주 분리 및 특성 분석)

  • Choi, Soo-Jeong;Park, Hyun-Jin;Lee, Jae-Hwa
    • Applied Chemistry for Engineering
    • /
    • v.26 no.5
    • /
    • pp.533-537
    • /
    • 2015
  • Micro-algae Chlorella vulgaris (C. vulgaris) is an important source for bio-diesel because of the high content of neutral lipids. In this study, we intended to induce mutants of C. vulgaris by UV-B irradiation. C. vulgaris was first exposed to UV-B for 1, 2, 3, 4, 5 min. As the UV-B exposure time increased, the cell viability and pigment content were decreased. Mutants of C. vulgaris were also induced through ultraviolet irradiation and two strains were selected with respect to lipid contents, where were named as 'UM10', 'UM15'. They were then cultivated in the same way as to the wild type. After 21 days of cultivation, the cell growth, dry cell weight, pigment contents, and lipid contents were measured for investigating characteristics of mutants. As a result, the cell growth and dry cell weight of both mutants increased about 1.4 and 1.5 times, respectively compared with those of wild type. In addition, chlorophyll and carotenoid contents were measured in order to investigate pigment contents in micro-algae through photosynthesis. It was shown that chlorophyll and carotenoid contents of both mutants decreased about 10% compared to those of wild type. Lipid contents in UM10 and UM15 increased about 1.2 and 1.5 times, respectively compared to that of wild type.

Effects of Combined Treatment of Aqueous Chlorine Dioxide and UV-C or Electron Beam Irradiation on Microbial Growth and Quality in Chicon during Storage (이산화염소수와 UV-C 또는 전자빔 병합처리가 치콘의 저장 중 미생물 성장과 품질에 미치는 영향)

  • Kang, Ji Hoon;Park, Jiyong;Oh, Deog Hwan;Song, Kyung Bin
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.41 no.11
    • /
    • pp.1632-1638
    • /
    • 2012
  • The effects of combined treatment of aqueous $ClO_2$ and UV-C or electron beam irradiation on microbial growth and quality in chicon during storage at $4^{\circ}C$ were investigated. Samples were treated separately with 50 ppm of $ClO_2$, 5 kJ/$m^2$ of UV-C, 2, 5, 7, and 10 kGy of electron beam irradiation, as well as a combination of $ClO_2$ and UV-C or 2 kGy of electron beam irradiation. The populations of total aerobic bacteria as well as yeast and molds in the chicon samples were determined following each treatment. The populations of total aerobic bacteria in the chicon samples decreased by 1.49~2.92 log CFU/g following combined treatment of $ClO_2$ and UV-C irradiation compared to the control, whereas the populations of yeast and molds decreased by 1.63~1.78 log CFU/g. On the contrary, following combined treatment of $ClO_2$ and electron beam irradiation, the populations of total aerobic bacteria as well as yeast and molds in the chicon samples were undetectable during storage. Color measurements indicated that Hunter $L^*$, $a^*$, and $b^*$ values were not significantly different among the treatments during storage. These results suggest that combined treatment of $ClO_2$ and electron beam irradiation can be useful for improving microbiological safety in chicon during storage.

Effect of Combined Treatment of Aqueous Chlorine Dioxide with Ultraviolet-C on the Quality of Red Chicory and Pak Choi during Storage (이산화염소수와 Ultraviolet-C 병합 처리에 따른 레드 치커리와 청경채의 저장 중 품질에 미치는 영향)

  • Kim, Hyun-Jin;Song, Hyeon-Jeong;Song, Kyung-Bin
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.40 no.2
    • /
    • pp.245-252
    • /
    • 2011
  • The combined effects of 10 kJ/$m^2$ ultraviolet-C (UV-C) with 50 ppm aqueous chlorine dioxide ($ClO_2$) on the qualities of red chicory and pak choi were examined. After the treatment, the samples were stored at $4{\pm}1^{\circ}C$ for 7 days. The combined treatment of $ClO_2$/UV-C reduced the initial populations of total aerobic bacteria in the red chicory and pak choi by 2.64 and 2.55 log CFU/g, respectively, compared to those of the control. Also, the populations of yeast and molds in the red chicory and pak choi by combined treatment were reduced by 2.41 and 2.00 log CFU/g, respectively. In addition, after 7 days of storage the total aerobic bacteria populations in the red chicory and pak choi were reduced to 3.71 and 3.93 log CFU/g, compared to 6.31 and 6.62 log CFU/g for the control, resulting in a significant decrease. Hunter L, a, and b values of red chicory and pak choi were not significantly different among the treatments. Non-thermal treatment caused a negligible change in sensory evaluation. These results suggest that the combined treatment of 50 ppm $ClO_2$ with 10 kJ/$m^2$ UV-C can be useful for maintaining the qualities of red chicory and pak choi.

Effects of Plant Extract containing Creams on UVB Radiation-induced Inflammatory Responses in Mice (쥐에서 자외선 B가 유도한 염증 반응에 대한 식물 추출물 함유 크림의 영향)

  • An, Sang-Mi; Lee, Seung-Jin;Park, Kwon-Moo;Koh, Jae-Sook;Boo, Yong-Chool
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.36 no.4
    • /
    • pp.271-280
    • /
    • 2010
  • Ultraviolet radiation (UV) is a major cause of photodamages to human skin and the immediate responses of the skin to UV include the erythema and edema. In an attempt to find effective UV-protecting agents to be used in cosmetics, a number of plant extracts were screened in the cell-based assays. Among the total of 38 plant extracts tested, 3 plant extracts derived from Sasa quelpaertensis, Althaea rosea, and Dryopteris crassirhizoma attenuated the UVB-induced cytotoxicity as well as melanin synthesis in cultured human epidermal melanocytes. The anti-inflammatory effects of these plant extracts were further examined in animal models. A control or test cream containing 1% of a plant extract was topically applied to ears of a C57BL/6 mouse or the dorsal skin of a SKH-1 hafirless mouse before and after the exposure to UVB. The change in ear thickness or dorsal skin redness due to UVB exposure was determined to monitor edema and erythema, respectively. All three test creams exhibited anti-inflammatory effects in both experiments. The creams containing Sasa quelpaertensis, Althaea rosea or Dryopteris crassirhizoma extract alleviated the UVB-induced edema response on day 4 by 53.8 %, 56.4 % and 31.1 %, respectively. They also inhibited the erythema formation on day 2 by 45.7 %, 34.1 % and 20.5 %, respectively. This study suggests that the selected plant extracts formulated in cosmetics may attenuate skin inflammation caused by overexposure to UV.

Protective effect of Thymus quinquecostatus extracts UVB-induced matrix metalloproteinase-1 via suppressing MAPKs phosphorylation in human keratinocyte (사람의 각질세포에서 UVB 유도에 따른 MMP-1의 발현 조절과 MAPKs 인산화에 타임 추출물이 미치는 효과)

  • Jung, Hana;Jeong, Hyun Ju;Shin, Kyounghee;Kim, Yung Sun;Moon, Jae Heon;Lee, Tae Hoon
    • Journal of Applied Biological Chemistry
    • /
    • v.61 no.4
    • /
    • pp.417-421
    • /
    • 2018
  • Ultraviolet rays are electromagnetic waves with a shorter wavelength than visible light, and ultraviolet rays that pass through the ozone layer are the main cause of skin aging. Chronic exposure of skin tissue to ultraviolet light activates the Mitogen-activated Protein Kinases (MAPKs) signaling pathways in human keratinocytes, resulting in increased production of matrix metalloproteinases (MMPs). In this study, we investigated the herbal extracts from Jeju Island on the anti-aging effect in human keratinocytes (HaCaTs) by ultraviolet stimulation. We examined that herb extract from Jeju Island were decreased in anti-aging activity on measuring the level of MMP-1 gene and protein expression in ultraviolet-induced keratinocytes. As a result, it was confirmed that Thymus quinquecostatus extract (TQE) significantly reduced the expression of MMP-1 in a dose-dependent manner by UV irradiated HaCaTs. According to our data, TQE significantly attenuated UV-induced phosphorylation of the MAPKs signaling elements ERK1/2, JNK1/2 and p38 proteins. These results suggest that the MAPKs pathway may contribute to the inhibitory effect of TQE on UV-induced MMP-1 production in human keratinocytes. Our results suggest that TQE may be a protective agent against skin aging by preventing UV-induced MMP-1 production.

Microbiological and Physicochemical Changes of Vegetable Juices (Angelica keiskei and Brassica loeracea var. acephala) Treated by UV Irradiation (UV 조사 신립초 및 케일 녹즙의 저장기간에 따른 미생물 및 이화학적 변화)

  • Kwon, Sang-Chul;Choi, Goo-Hee;Yu, Kwang-Won;Lee, Kyung-Haeng
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.39 no.7
    • /
    • pp.1030-1037
    • /
    • 2010
  • A fresh juice has become a new functional food available for dieting and health. However, the shelf-life of vegetable juice is very short because of the absence of heat pasteurization process. To elongate the shelf-life of vegetable juices, such as Angelica keiskei and Brassica loeracea var. acephala, the changes of microbiological, chemical and sensory property by UV irradiation were investigated. The total aerobic bacterial numbers of A. keiskei and B. loeracea var. acephala vegetable juices were $3.2{\times}10^5$ and $7.0{\times}10^4\;CFU/mL$, respectively, after wring process. However, the numbers were $3.6{\times}10^3{\sim}9.7{\times}10^3$ and $3.7{\times}10^3{\sim}2.7{\times}10^4\;CFU/mL$ after UV treatment on wring juice, and this lower microbial number was maintained during storage. The number of coliform bacteria also reduced significantly by UV treatment, and the bactericidal effect was higher when the flow rate is slower. The increase of lightness and yellowness, and decrease of redness were observed after treatment of UV on both vegetable juices, but the differences were not significant between flow rates. The ascorbic acid contents of vegetable juices were reduced by UV irradiation regardless of flow rate, and storage. Overall acceptance in sensory analysis revealed that there was no significant difference between the control and vegetable juice irradiated UV at 0 days, but sample with UV treatment showed higher score at 3 days. Therefore, UV treatment on vegetable juice can elongate the shelf-life without any problems in flavor and color.

The Anti-melanogenic Effect of Whitening Agent Containing Arbutin and ${\beta}-Glucosidase$ (알부틴과 베타-글루코시다제를 함유하는 미백 화장품의 항멜라닌 효과)

  • Lew Bark-Lin;Ryou Ji-Ho;Lee Mu-Hyoung
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.30 no.3 s.47
    • /
    • pp.415-418
    • /
    • 2004
  • Arbutin is a glycosylated hydroquinone found at high concentration in certain plants capable of surviving extreme and sustained dehydration. It has been reported to have an inhibitory effect of melanogenesis and to be hydrolyzed easily to yield glucose and hydroquinone by ${\beta}-glucosidase.$ While hydroquinone also has an anti-melanogenic effect, however, is carefully used as a topical whitening agent because of side effects. The present study was undertaken to examine the inhibitory effect of an whitening agent containing arbutin and ${\beta}-glucosidase$ on UV radiation induced pigmentation in human skin. Experimental subjects were UVB-irradiated on the back. UVB-irradiated areas were assigned to three groups: arbutin and ${\beta}-glucosidase$ treated group, vehicle control, and no-application control. Arbutin and ${\beta}-glucosidase$ treatment inhibited pigmentation by 50.17 percent, compared with the controls (N : 10: P<0.05). These results suggest that the whitening agent containing arbutin and ${\beta}-glucosidase$may be used as an agent to inhibit melanin formation induced by UV radiation.