Browse > Article
http://dx.doi.org/10.3839/jabc.2018.059

Protective effect of Thymus quinquecostatus extracts UVB-induced matrix metalloproteinase-1 via suppressing MAPKs phosphorylation in human keratinocyte  

Jung, Hana (Department of Applied Chemistry, Kyung Hee University)
Jeong, Hyun Ju (Agricultural Corporation JEJUWOODA Co., Ltd)
Shin, Kyounghee (Nature in Lab.Inc)
Kim, Yung Sun (Agricultural Corporation JEJUWOODA Co., Ltd)
Moon, Jae Heon (Nature in Lab.Inc)
Lee, Tae Hoon (Department of Applied Chemistry, Kyung Hee University)
Publication Information
Journal of Applied Biological Chemistry / v.61, no.4, 2018 , pp. 417-421 More about this Journal
Abstract
Ultraviolet rays are electromagnetic waves with a shorter wavelength than visible light, and ultraviolet rays that pass through the ozone layer are the main cause of skin aging. Chronic exposure of skin tissue to ultraviolet light activates the Mitogen-activated Protein Kinases (MAPKs) signaling pathways in human keratinocytes, resulting in increased production of matrix metalloproteinases (MMPs). In this study, we investigated the herbal extracts from Jeju Island on the anti-aging effect in human keratinocytes (HaCaTs) by ultraviolet stimulation. We examined that herb extract from Jeju Island were decreased in anti-aging activity on measuring the level of MMP-1 gene and protein expression in ultraviolet-induced keratinocytes. As a result, it was confirmed that Thymus quinquecostatus extract (TQE) significantly reduced the expression of MMP-1 in a dose-dependent manner by UV irradiated HaCaTs. According to our data, TQE significantly attenuated UV-induced phosphorylation of the MAPKs signaling elements ERK1/2, JNK1/2 and p38 proteins. These results suggest that the MAPKs pathway may contribute to the inhibitory effect of TQE on UV-induced MMP-1 production in human keratinocytes. Our results suggest that TQE may be a protective agent against skin aging by preventing UV-induced MMP-1 production.
Keywords
Human Keratinocytes; Matrix metalloproteinases; Mitogen-activated protein kinase; Thymus quinquecostatus extract; Ultraviolet-B;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Jenkins G (2002) Molecular mechanisms of skin ageing. Mech Ageing Dev 123: 801-810   DOI
2 Pittayapruek P, Meephansan J, Prapapan O, Komine M, Ohtsuki M (2016) Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int J Mol Sci 17: E868   DOI
3 Ho JH, Lee YH, Park JS, Jun WJ, Kim HK, Hong BS, Shin DH, Cho HY (2005) Protective effects of aucubin isolated from eucommia ulmoides against UVBinduced oxidative stress in human skin fibroblasts. Biol Pharm Bull 28: 1244-1248   DOI
4 Parkinson LG, Toro A, Zhao H, Brown K, Tebbutt SJ, Granville DJ (2015) Grnazyme B mediates both direct and indirect cleavage of extracellular matrix in skin after chronic low-dose ultraviolet light irradiation. Aging Cell 14: 67-77   DOI
5 McCawley LJ, Matrisian LM (2001) Matrix metalloproteinases: they are not just for matrix anymore. Curr Opin. Cell Biol 13 534-540   DOI
6 Lauer-Fields JL, Juska D, Fields GB (2002) Matrix metalloproteinases and collagen catabolism. Biopolymers 66: 19-32   DOI
7 Visse R, Nagase H (2003) Matrix metalloproteinases and tissue inhibitors of metalloproteinases. Structure, function, and biochemistry. Circ Res 92: 827-839   DOI
8 Brennan M, Bhatti H, Nerusu KC, Bhagavathula N, Kang S, Fisher GJ (2003) Matrix metalloproteinase-1 is the major collagenolytic enzyme responsible for collagen damage in UV-irradiated human skin. Photochem Photobiol 78: 43-48   DOI
9 Reunanen N, Westermarck J, Hakkinen L, Holmstrom TH, Elo I, Eriksson JE (1998) Enhancement of fibroblast collagenase (matrix metalloproteinase-1) gene expression by ceramide is mediated by extracellular signal-regulated and stress-activated protein kinase pathways. J Biol Chem 273: 5137-5145   DOI
10 Chang L, Karin M (2001) Mammalian MAP kinase signalling cascades. Nature 410: 37-40   DOI
11 Khan N, Syed DN, Pal HC, Mukhtar H, Afaq F (2012) Pomegranate fruit extract inhibits UVB-induced inflammation and proliferation by modulating NF-kB and MAPK signaling pathways in mouse skin. Photochem Photobiol 88: 1126-1134   DOI
12 Shin S, Kim JH (2004) Antifungal activities of essential oil from Thymus quinquecoststus and T. magnus. Planta Med 70: 1090-1092   DOI
13 Kim YS, Hwang JW, Kang SH, Kim EH, Jeon YJ, Jeong JH, Kim HR, Moon SH, Jeon BT, Park PJ (2014) Thymol from Thymus quinquecostatus Celak. protects against tert-butyl hydroperoxide-induced oxidative stress in Chang cells. J Nat Med 68: 154-162   DOI
14 Pasparakis M, Courtois G, Hafner M, Schmidt-Supprian M, Nenci A, Toksoy A, Krampert M, Goebeler M, Gillitzer R, Israel A, Krieg T, Rajewsky K, Haase I (2002) TNF-mediated inflammatory skin disease in mice with epidermisspecific deletion of IKK2, Nature 417: 861-866   DOI
15 Bell S, Degitz K, Quirling M, Jilg N, Page S, Brand K (2003) Involvement of NFkappaB signalling in skin physiology and disease, Cell Signal 15: 1-7   DOI