• Journal of Microbiology
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• v.44 no.3
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• pp.269-275
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• 2006

Atmospheric-pressure cold plasma (APCP) using helium/oxygen was developed and tested as a suitable sterilization method in a clinical environment. The sterilizing effect of this method is not due to UV light, which is known to be the major sterilization factor of APCP, but instead results from the action of reactive oxygen radicals. Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae deposited on a nitrocellulose filter membrane or Bacillus subtilis spores deposited on polypropylene plates were exposed to helium/oxygen plasma generated with AC input power at 10 kHz, 6 kV. After Plasma treatment, nitrocellulose filter membranes were overlaid on fresh solid media and CFUs were counted after incubation overnight. D-values were 18 sec for E. coli, 19 sec for S. aureus, 1 min 55 sec for S. cerevisiae, and 14 min for B. subtilis spores. D-values of bacteria and yeast were dependent on the initial inoculation concentration, while the D-value of B. subtilis spores showed no correlation. When treated cells were observed with a scanning electron microscope, E. coli was more heavily damaged than S. aureus, S. cevevisiae exhibited peeling, and B. subtilis spores exhibited shrunken morphology. Results showed that APCP using helium/oxygen has many advantages as a sterilization method, especially in a clinical environment with conditions such as stable temperature, unlimited sample size, and no harmful gas production.

• Journal of the Korean Society of Radiology
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• v.14 no.5
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• pp.695-703
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• 2020

The use of medical devices is increasing due to the development of medical technology. Among medical devices, it is often used in the human body for graft and treatment. Therefore, in medical institutions, various sterilization methods according to the type and material of medical devices are applied to prevent infection. Hydrophosphite (HA) materials are the most popular in bone grafts. We would like to present a sterilization method using Q-switch Nd:YAG laser with high output energy among non-ionizing radiation suitable for small medical devices. In this study, sterilization power was most ideally shown at UV wavelengths of 1.5 W, 266 nm, and 10 pulses. Different wavelength bands; infrared and visible light; showed passive sterilization, and ultraviolet A and C showed differences in sterilization according to the pulse. In laser sterilization were differences that found according to the wavelength bands and pulses.

• Journal of Environmental Science International
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• v.13 no.4
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• pp.403-412
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• 2004

Waste water-soluble cutting oil was treated with WI type #1 and WI type #2. The properties of the original water-soluble cutting oil were pH=l0.4, viscosity=1.4cP, CODcr=44,750 ppm, and TOC=10,569 ppm. However, the properties of the oil used for more than 3 months were changed to pH=7.82, viscosity=2.1cP, CODcr=151,000 ppm, and TOC=74,556 ppm. It might be attributed to the fact that molecular chains were cut due to thermal oxidation and impurities such as metal chips were incorporated in to the oil during the operation processes. To prevent the putrefaction of oil, the sterilization effect of ozone and UV on the microorganism in the oil was investigated. Ozone treatment showed that 99.99% of the microorganism was annihilated with 30 minutes contact time and 60 minutes were necessary for the same effect when UV was used. Ozone treatment could cut molecular chains of the oil due to strong sterilization power, which was evidenced by the increase of TOC from 25,132 ppm at instantaneous contact to 28,888 ppm at 30 minutes contact time. However, UV treatment didn't show severe changes in TOC values and thus, seemed to cause of severe cut of molecular chains. When the activated carbon was used to treat the waste water-soluble cutting oil, TOC decreased to 25,417 ppm with 0.lg carbon and to 15,946 ppm with 5.0g carbon. This results indicated that the waste oil of small molecular chains could be eliminated by adsorption. From the results, it could be concluded that these treatment techniques could be proposed to remove the waste oil of small molecular chains resulting in the degradation of the oil properties. In addition, these experimental results could be used for the correlation with future works such as investigation of the molecular distribution according to the sizes, lengths, and molecular weight of the chains.

• Proceedings of the Korean Society of Marine Engineers Conference
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• 2011.06a
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• pp.196-196
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• 2011

최근 연구가 활발히 진행되고 있는 UV-LED를 활용한 살균 기술에 있어서 제조 공정상 고가를 유지하는 UV-LED는 그 살균 시스템의 발전에 큰 지장이 되고 있다. 이러한 문제점을 해결하기 위하여 본 연구에서는 LED의 갯수는 줄어들지만 살균 효율은 유지될 수 있는 시스템을 살펴보았다. 이 시스템에서는 UV-LED 갯수를 1개로 유지하며 이를 LED의 방사 Angle에 맞추어 꺾은 상태로 회전하게 되고 그 조광 범위가 향상된다. 또한 그 상태에서 조광구역과의 거리와 회전 시키는 스피드, Angle의 변화에 따른 최적의 위치 및 속도를 찾아보았다. 현재는 일반 가시광 LED를 이용하여 LED를 회전했을 때의 조광 범위와 조광의 정도에 대해서만 서술하였고 추후 UV-LED를 통하여 더 활발한 연구를 진행하려 한다.

• Journal of Mushroom
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• v.19 no.3
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• pp.210-215
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• 2021

For sterilization of microorganisms of the Listeria genus contaminating enoki mushroom, pilot mushroom grower equipped with air sterilization devices were developed. Sterilization experiments were performed using physical and chemical treatments. Internal temperature and humidity were controlled, maintaining 6.62℃±0.30 in the upper shelves, 6.46℃±0.24 in the middle shelves, and 6.48℃±0.25 in the lower shelves. Humidities were 79.97%±4.42, 79.43%±4.06, and 79.94±4.30%, respectively, with a temperature setting of 6.5℃, and a relative humidity of 75%. A suitable enoki mushroom cultivation stage for air sterilizer application was during the growth stage, with temperature in the 6.5~8.5℃ range, and humidity of 70~80%. At these same internal conditions, the ozone concentration in the mushroom cultivator was found to be 160 ppb during ion-cluster generator operation. After physical sterilization, the Listeria innocua survival rate was 0.1 to 0.9% using ion cluster sterilization, and 9.3 to 10.6% using UV air sterilization. The Listeria innocua survival rates on different materials were 9.3~10.6% on the metal specimen, and 9.9~16.2% on the plastic wrapper. The survival rate was particularly high on the rough side of the plastic wrapper. Ion cluster air sterilization is a labor-saving and effective method for suppressing the occurrence of Listeria bacteria on mushroom growers walls and shelves. For the plastic wrapper, chemical sterilization is more effective than physical sterilization.

• Applied Chemistry for Engineering
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• v.25 no.4
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• pp.386-391
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• 2014

Atmospheric-pressure nonthermal corona discharge plasma was applied to the sterilization of biologically contaminated scoria powder. Escherichia coli (E. coli) culture solution was uniformly sprayed throughout the scoria powder for artificial inoculation, which was well mixed to ensure uniformity of the batch. The effect of the key parameters such as discharge power, treatment time, type of gas and electrode distance on the sterilization efficiency was examined and discussed. The experimental results revealed that the plasma treatment was very effective for the sterilization of scoria powder; 5-min treatment at 15 W could sterilize more than 99.9% of E. coli inoculated into the scoria powder. Increasing the discharge power, treatment time or applied voltage led to an improvement in the sterilization efficiency. The effect of type of gas on the sterilization efficiency was in order of oxygen, synthetic air (20% oxygen) and nitrogen from high to low. The inactivation of E. coli under the influence of corona discharge plasma can be explained by cell membrane erosion or etching resulting from UV and reactive oxidizing species (oxygen radical, OH radical, ozone, etc.), and the destruction of E. coli cell membrane by the physical action of numerous corona streamers.

• Proceedings of the Korea Information Processing Society Conference
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• 2022.11a
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• pp.794-796
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• 2022

본 논문은 에어컨 토출구 살균조명용 UV-LED 에 관한 연구이다. 서론에서는 에어컨 토출구에 증식하는 곰팡이의 문제점과 원인에 대해 조사 분석함으로써 연구의 목적을 설명하였다. 본론에서는 내부를 살균하며 동시에 무드조명의 역할을 수행함으로써 얻을 수 있는 장점과 작동방식에 대하여 설명하고, 가능성을 확인하기 위한 실험을 계획하였다. 실험계획과 실험결과에서는 이전 다른 실험과의 차이점과 실험계획을 설명하고, 실험결과에 대해 설명하며 이번 실험에서의 한계점 및 보안할 점도 함께 설명하였다. UV-LED 의 설치 위치를 예시를 들며 설명하였다. 결론에서는 본 논문에서 설명한 연구의 내용을 종합하고, 향후 연구의 진행방향과 추가적으로 필요한 실험 및 적절한 UV-LED 의 파장선택에 대해서 기술하였다.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.19 no.8
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• pp.19-23
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• 2005

The target of this research is a design of constant and high efficiency driving system for a low pressure UV lamp. An UV lamp system is one of wide range electrical equipments for semiconductor manufacturing and sterilization, etc... It is essential the technique of constant output for high added value device. A design target of driving system for low pressure UV lamp of conversion efficiency is 90[%], UV lamp of output stability within ${\pm}7.5[%]$, and lamp power is over 200[W]. The results meet the target of this study well, and have a benefit of domestic market occupation and enable to export. And if protection circuits were developed, it increases the stability of a electronic ballast for UV lamps.

• Journal of Korean Society of Water and Wastewater
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• v.28 no.5
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• pp.541-548
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• 2014

The pickling brine generated from the salting process of kimchi production is difficult to treat biologically due to very high content of salt. When pickling brine is treated and discharged, it cannot satisfy the criteria for effluent water quality in clean areas, while resources such as the salt to be recycled and the industrial water are wasted. However, sterilization by ozone, UV and photocatalyst is expensive installation costs and operating costs when considering the small kimchi manufacturers. Therefore there is a need to develop economical process. The study was conducted on the sterilization efficiency of the pickling brine using electrochemical processing. The electrochemical treatment of organic matters has advantages over conventional methods such as active carbon absorption process, chemical oxidation, and biological treatment because the response speed is faster and it does not require expensive, harmful oxidizing agents. This study were performed to examine the possibility of electrochemical treatment for the efficient processing of pickling brine and evaluated the performance of residual chlorine for the microbial sterilization.

• Journal of the Korean Society of Radiology
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• v.14 no.6
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• pp.833-839
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• 2020

Globally, infection prevention and social awareness have been greatly changed by the severe acute respiratory syndrome coronavirus, and as a result, the infection control guidelines and procedures for patients with high exposure to hospital-acquired infection are further strengthened and management and monitoring are more thorough. In order to prevent infection, sterilization should be carried out with the highest priority, and we will find a sterilization method that is low in cost, easy to install, and easy to operate, to present appropriate sterilization effects. In this study, the UV sterilizer was used to contaminate the caries bacteria with an output of 4 W and irradiation time of 60, 150, and 300 sec, and the laser was irradiated with outputs of 0.8 and 1.5 W at wavelengths of 266 and 355 nm, respectively. Ultraviolet sterilizer showed safety in infection prevention at over 150 sec, and laser showed safety in prevention at 1.5 W, 0.8 W, and 266 nm. As a result, the higher the output and the wavelength closer to 253.7 nm, the better the sterilization effect.